Characterization of protein-protein interactions between the nucleocapsid protein and membrane protein of the SARS coronavirus.
Identifieur interne : 005429 ( Main/Exploration ); précédent : 005428; suivant : 005430Characterization of protein-protein interactions between the nucleocapsid protein and membrane protein of the SARS coronavirus.
Auteurs : Runtao He [Canada] ; Andrew Leeson ; Melissa Ballantine ; Anton Andonov ; Lindsay Baker ; Frederick Dobie ; Yan Li ; Nathalie Bastien ; Heinz Feldmann ; Ute Strocher ; Steven Theriault ; Todd Cutts ; Jingxin Cao ; Timothy F. Booth ; Frank A. Plummer ; Shaun Tyler ; Xuguang LiSource :
- Virus research [ 0168-1702 ] ; 2004.
Descripteurs français
- KwdFr :
- Assemblage viral, Cartographie d'interactions entre protéines, Conformation des protéines, Délétion de séquence, Liaison aux protéines, Protéines de la matrice virale (métabolisme), Protéines nucléocapside (), Protéines nucléocapside (métabolisme), Protéines virales (métabolisme), Sites de fixation, Techniques de double hybride, Virus du SRAS (métabolisme), Virus du SRAS (physiologie).
- MESH :
- métabolisme : Protéines de la matrice virale, Protéines nucléocapside, Protéines virales, Virus du SRAS.
- physiologie : Virus du SRAS.
- Assemblage viral, Cartographie d'interactions entre protéines, Conformation des protéines, Délétion de séquence, Liaison aux protéines, Protéines nucléocapside, Sites de fixation, Techniques de double hybride.
English descriptors
- KwdEn :
- Binding Sites, Nucleocapsid Proteins (chemistry), Nucleocapsid Proteins (metabolism), Protein Binding, Protein Conformation, Protein Interaction Mapping, SARS Virus (metabolism), SARS Virus (physiology), Sequence Deletion, Two-Hybrid System Techniques, Viral Matrix Proteins (metabolism), Viral Proteins (metabolism), Virus Assembly.
- MESH :
- chemical , chemistry : Nucleocapsid Proteins.
- chemical , metabolism : Nucleocapsid Proteins, Viral Matrix Proteins, Viral Proteins.
- metabolism : SARS Virus.
- physiology : SARS Virus.
- Binding Sites, Protein Binding, Protein Conformation, Protein Interaction Mapping, Sequence Deletion, Two-Hybrid System Techniques, Virus Assembly.
Abstract
The human coronavirus, associated with severe acute respiratory syndrome (SARS-CoV), was identified and molecularly characterized in 2003. Sequence analysis of the virus indicates that there is only 20% amino acid (aa) identity with known coronaviruses. Previous studies indicate that protein-protein interactions amongst various coronavirus proteins are critical for viral assembly. Yet, little sequence homology between the newly identified SARS-CoV and those previously studied coronaviruses suggests that determination of protein-protein interaction and identification of amino acid sequences, responsible for such interaction in SARS-CoV, are necessary for the elucidation of the molecular mechanism of SARS-CoV replication and rationalization of anti-SARS therapeutic intervention. In this study, we employed mammalian two-hybrid system to investigate possible interactions between SARS-CoV nucleocapsid (N) and the membrane (M) proteins. We found that interaction of the N and M proteins takes place in vivo and identified that a stretch of amino acids (168-208) in the N protein may be critical for such protein-protein interactions. Importantly, the same region has been found to be required for multimerization of the N protein (He et al., 2004) suggesting this region may be crucial in maintaining correct conformation of the N protein for self-interaction and interaction with the M protein.
DOI: 10.1016/j.virusres.2004.05.002
PubMed: 15351485
Affiliations:
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Le document en format XML
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<series><title level="j">Virus research</title>
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<term>Nucleocapsid Proteins (metabolism)</term>
<term>Protein Binding</term>
<term>Protein Conformation</term>
<term>Protein Interaction Mapping</term>
<term>SARS Virus (metabolism)</term>
<term>SARS Virus (physiology)</term>
<term>Sequence Deletion</term>
<term>Two-Hybrid System Techniques</term>
<term>Viral Matrix Proteins (metabolism)</term>
<term>Viral Proteins (metabolism)</term>
<term>Virus Assembly</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Assemblage viral</term>
<term>Cartographie d'interactions entre protéines</term>
<term>Conformation des protéines</term>
<term>Délétion de séquence</term>
<term>Liaison aux protéines</term>
<term>Protéines de la matrice virale (métabolisme)</term>
<term>Protéines nucléocapside ()</term>
<term>Protéines nucléocapside (métabolisme)</term>
<term>Protéines virales (métabolisme)</term>
<term>Sites de fixation</term>
<term>Techniques de double hybride</term>
<term>Virus du SRAS (métabolisme)</term>
<term>Virus du SRAS (physiologie)</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nucleocapsid Proteins</term>
<term>Viral Matrix Proteins</term>
<term>Viral Proteins</term>
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<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term>
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<term>Protéines nucléocapside</term>
<term>Protéines virales</term>
<term>Virus du SRAS</term>
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<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term>
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<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term>
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<term>Protein Binding</term>
<term>Protein Conformation</term>
<term>Protein Interaction Mapping</term>
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<term>Two-Hybrid System Techniques</term>
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<term>Cartographie d'interactions entre protéines</term>
<term>Conformation des protéines</term>
<term>Délétion de séquence</term>
<term>Liaison aux protéines</term>
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<front><div type="abstract" xml:lang="en">The human coronavirus, associated with severe acute respiratory syndrome (SARS-CoV), was identified and molecularly characterized in 2003. Sequence analysis of the virus indicates that there is only 20% amino acid (aa) identity with known coronaviruses. Previous studies indicate that protein-protein interactions amongst various coronavirus proteins are critical for viral assembly. Yet, little sequence homology between the newly identified SARS-CoV and those previously studied coronaviruses suggests that determination of protein-protein interaction and identification of amino acid sequences, responsible for such interaction in SARS-CoV, are necessary for the elucidation of the molecular mechanism of SARS-CoV replication and rationalization of anti-SARS therapeutic intervention. In this study, we employed mammalian two-hybrid system to investigate possible interactions between SARS-CoV nucleocapsid (N) and the membrane (M) proteins. We found that interaction of the N and M proteins takes place in vivo and identified that a stretch of amino acids (168-208) in the N protein may be critical for such protein-protein interactions. Importantly, the same region has been found to be required for multimerization of the N protein (He et al., 2004) suggesting this region may be crucial in maintaining correct conformation of the N protein for self-interaction and interaction with the M protein.</div>
</front>
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<affiliations><list><country><li>Canada</li>
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<name sortKey="Booth, Timothy F" sort="Booth, Timothy F" uniqKey="Booth T" first="Timothy F" last="Booth">Timothy F. Booth</name>
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<name sortKey="Leeson, Andrew" sort="Leeson, Andrew" uniqKey="Leeson A" first="Andrew" last="Leeson">Andrew Leeson</name>
<name sortKey="Li, Xuguang" sort="Li, Xuguang" uniqKey="Li X" first="Xuguang" last="Li">Xuguang Li</name>
<name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name>
<name sortKey="Plummer, Frank A" sort="Plummer, Frank A" uniqKey="Plummer F" first="Frank A" last="Plummer">Frank A. Plummer</name>
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<name sortKey="Theriault, Steven" sort="Theriault, Steven" uniqKey="Theriault S" first="Steven" last="Theriault">Steven Theriault</name>
<name sortKey="Tyler, Shaun" sort="Tyler, Shaun" uniqKey="Tyler S" first="Shaun" last="Tyler">Shaun Tyler</name>
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<country name="Canada"><noRegion><name sortKey="He, Runtao" sort="He, Runtao" uniqKey="He R" first="Runtao" last="He">Runtao He</name>
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