SARS coronavirus induces apoptosis in Vero E6 cells.
Identifieur interne : 005131 ( Main/Exploration ); précédent : 005130; suivant : 005132SARS coronavirus induces apoptosis in Vero E6 cells.
Auteurs : Huimin Yan [République populaire de Chine] ; Gengfu Xiao ; Jiamin Zhang ; Yuanyang Hu ; Fang Yuan ; David K. Cole ; Congyi Zheng ; George F. GaoSource :
- Journal of medical virology [ 0146-6615 ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux, Apoptose, Cellules Vero, Chromatine (ultrastructure), Coloration et marquage, Effet cytopathogène viral, Fluorescence, Fragmentation de l'ADN, Immunochimie, Microscopie électronique, Mitochondries (anatomopathologie), Mitochondries (ultrastructure), Noyau de la cellule (anatomopathologie), Noyau de la cellule (ultrastructure), Virus du SRAS (croissance et développement), Virus du SRAS (pathogénicité).
- MESH :
- anatomopathologie : Mitochondries, Noyau de la cellule.
- croissance et développement : Virus du SRAS.
- pathogénicité : Virus du SRAS.
- ultrastructure : Animaux, Apoptose, Cellules Vero, Chromatine, Coloration et marquage, Effet cytopathogène viral, Fluorescence, Fragmentation de l'ADN, Immunochimie, Microscopie électronique, Mitochondries, Noyau de la cellule.
English descriptors
- KwdEn :
- Animals, Apoptosis, Cell Nucleus (pathology), Cell Nucleus (ultrastructure), Chlorocebus aethiops, Chromatin (ultrastructure), Cytopathogenic Effect, Viral, DNA Fragmentation, Fluorescence, Immunochemistry, Microscopy, Electron, Mitochondria (pathology), Mitochondria (ultrastructure), SARS Virus (growth & development), SARS Virus (pathogenicity), Staining and Labeling, Vero Cells.
- MESH :
- chemical , ultrastructure : Chromatin.
- growth & development : SARS Virus.
- pathogenicity : SARS Virus.
- pathology : Cell Nucleus, Mitochondria.
- ultrastructure : Cell Nucleus, Mitochondria.
- Animals, Apoptosis, Chlorocebus aethiops, Cytopathogenic Effect, Viral, DNA Fragmentation, Fluorescence, Immunochemistry, Microscopy, Electron, Staining and Labeling, Vero Cells.
Abstract
Severe acute respiratory syndrome (SARS) is an emerging infectious disease. Its etiological agent has been convincingly identified as a new member of family Coronaviridae (SARS-CoV). It causes serious damage to the respiratory system yet the mechanism is not clear. Infection-induced apoptosis or necrosis is suspected but no direct evidence for this yet exists. To date, Vero E6 cells are the only cell line that could be used to replicate the virus with obvious CPE (cytopathic effect) in vitro. It is known for some viruses (including members of family Coronaviridae) that CPE can be caused either by virus-induced apoptosis (active death) or cell necrosis (passive death). In this study, we examined the apoptosis in the SARS-CoV infected Vero E6 cells. Indeed, the results do show that the CPE was induced by apoptosis rather than necrosis, shown by typical DNA fragmentation, through the existence of apoptotic bodies and swollen mitochondria. This observation has some implications for the SARS-CoV pathogenicity: SARS-CoV does induce apoptosis in cell cultures and might have the same effect in vivo, responsible for the severe damage of the respiratory system.
DOI: 10.1002/jmv.20094
PubMed: 15170624
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002D68
- to stream PubMed, to step Curation: 002D68
- to stream PubMed, to step Checkpoint: 002A89
- to stream Ncbi, to step Merge: 000838
- to stream Ncbi, to step Curation: 000838
- to stream Ncbi, to step Checkpoint: 000838
- to stream Main, to step Merge: 005504
- to stream Main, to step Curation: 005131
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">SARS coronavirus induces apoptosis in Vero E6 cells.</title>
<author><name sortKey="Yan, Huimin" sort="Yan, Huimin" uniqKey="Yan H" first="Huimin" last="Yan">Huimin Yan</name>
<affiliation wicri:level="4"><nlm:affiliation>School of Life Sciences, Wuhan University, Wuhan, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>School of Life Sciences, Wuhan University, Wuhan</wicri:regionArea>
<placeName><settlement type="city">Wuhan</settlement>
<region type="région">Hubei</region>
</placeName>
<orgName type="university">Université de Wuhan</orgName>
</affiliation>
</author>
<author><name sortKey="Xiao, Gengfu" sort="Xiao, Gengfu" uniqKey="Xiao G" first="Gengfu" last="Xiao">Gengfu Xiao</name>
</author>
<author><name sortKey="Zhang, Jiamin" sort="Zhang, Jiamin" uniqKey="Zhang J" first="Jiamin" last="Zhang">Jiamin Zhang</name>
</author>
<author><name sortKey="Hu, Yuanyang" sort="Hu, Yuanyang" uniqKey="Hu Y" first="Yuanyang" last="Hu">Yuanyang Hu</name>
</author>
<author><name sortKey="Yuan, Fang" sort="Yuan, Fang" uniqKey="Yuan F" first="Fang" last="Yuan">Fang Yuan</name>
</author>
<author><name sortKey="Cole, David K" sort="Cole, David K" uniqKey="Cole D" first="David K" last="Cole">David K. Cole</name>
</author>
<author><name sortKey="Zheng, Congyi" sort="Zheng, Congyi" uniqKey="Zheng C" first="Congyi" last="Zheng">Congyi Zheng</name>
</author>
<author><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F" last="Gao">George F. Gao</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2004">2004</date>
<idno type="RBID">pubmed:15170624</idno>
<idno type="pmid">15170624</idno>
<idno type="doi">10.1002/jmv.20094</idno>
<idno type="wicri:Area/PubMed/Corpus">002D68</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002D68</idno>
<idno type="wicri:Area/PubMed/Curation">002D68</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002D68</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002A89</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002A89</idno>
<idno type="wicri:Area/Ncbi/Merge">000838</idno>
<idno type="wicri:Area/Ncbi/Curation">000838</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000838</idno>
<idno type="wicri:doubleKey">0146-6615:2004:Yan H:sars:coronavirus:induces</idno>
<idno type="wicri:Area/Main/Merge">005504</idno>
<idno type="wicri:Area/Main/Curation">005131</idno>
<idno type="wicri:Area/Main/Exploration">005131</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">SARS coronavirus induces apoptosis in Vero E6 cells.</title>
<author><name sortKey="Yan, Huimin" sort="Yan, Huimin" uniqKey="Yan H" first="Huimin" last="Yan">Huimin Yan</name>
<affiliation wicri:level="4"><nlm:affiliation>School of Life Sciences, Wuhan University, Wuhan, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>School of Life Sciences, Wuhan University, Wuhan</wicri:regionArea>
<placeName><settlement type="city">Wuhan</settlement>
<region type="région">Hubei</region>
</placeName>
<orgName type="university">Université de Wuhan</orgName>
</affiliation>
</author>
<author><name sortKey="Xiao, Gengfu" sort="Xiao, Gengfu" uniqKey="Xiao G" first="Gengfu" last="Xiao">Gengfu Xiao</name>
</author>
<author><name sortKey="Zhang, Jiamin" sort="Zhang, Jiamin" uniqKey="Zhang J" first="Jiamin" last="Zhang">Jiamin Zhang</name>
</author>
<author><name sortKey="Hu, Yuanyang" sort="Hu, Yuanyang" uniqKey="Hu Y" first="Yuanyang" last="Hu">Yuanyang Hu</name>
</author>
<author><name sortKey="Yuan, Fang" sort="Yuan, Fang" uniqKey="Yuan F" first="Fang" last="Yuan">Fang Yuan</name>
</author>
<author><name sortKey="Cole, David K" sort="Cole, David K" uniqKey="Cole D" first="David K" last="Cole">David K. Cole</name>
</author>
<author><name sortKey="Zheng, Congyi" sort="Zheng, Congyi" uniqKey="Zheng C" first="Congyi" last="Zheng">Congyi Zheng</name>
</author>
<author><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F" last="Gao">George F. Gao</name>
</author>
</analytic>
<series><title level="j">Journal of medical virology</title>
<idno type="ISSN">0146-6615</idno>
<imprint><date when="2004" type="published">2004</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Apoptosis</term>
<term>Cell Nucleus (pathology)</term>
<term>Cell Nucleus (ultrastructure)</term>
<term>Chlorocebus aethiops</term>
<term>Chromatin (ultrastructure)</term>
<term>Cytopathogenic Effect, Viral</term>
<term>DNA Fragmentation</term>
<term>Fluorescence</term>
<term>Immunochemistry</term>
<term>Microscopy, Electron</term>
<term>Mitochondria (pathology)</term>
<term>Mitochondria (ultrastructure)</term>
<term>SARS Virus (growth & development)</term>
<term>SARS Virus (pathogenicity)</term>
<term>Staining and Labeling</term>
<term>Vero Cells</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Apoptose</term>
<term>Cellules Vero</term>
<term>Chromatine (ultrastructure)</term>
<term>Coloration et marquage</term>
<term>Effet cytopathogène viral</term>
<term>Fluorescence</term>
<term>Fragmentation de l'ADN</term>
<term>Immunochimie</term>
<term>Microscopie électronique</term>
<term>Mitochondries (anatomopathologie)</term>
<term>Mitochondries (ultrastructure)</term>
<term>Noyau de la cellule (anatomopathologie)</term>
<term>Noyau de la cellule (ultrastructure)</term>
<term>Virus du SRAS (croissance et développement)</term>
<term>Virus du SRAS (pathogénicité)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="ultrastructure" xml:lang="en"><term>Chromatin</term>
</keywords>
<keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Mitochondries</term>
<term>Noyau de la cellule</term>
</keywords>
<keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Cell Nucleus</term>
<term>Mitochondria</term>
</keywords>
<keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Cell Nucleus</term>
<term>Mitochondria</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Apoptosis</term>
<term>Chlorocebus aethiops</term>
<term>Cytopathogenic Effect, Viral</term>
<term>DNA Fragmentation</term>
<term>Fluorescence</term>
<term>Immunochemistry</term>
<term>Microscopy, Electron</term>
<term>Staining and Labeling</term>
<term>Vero Cells</term>
</keywords>
<keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Animaux</term>
<term>Apoptose</term>
<term>Cellules Vero</term>
<term>Chromatine</term>
<term>Coloration et marquage</term>
<term>Effet cytopathogène viral</term>
<term>Fluorescence</term>
<term>Fragmentation de l'ADN</term>
<term>Immunochimie</term>
<term>Microscopie électronique</term>
<term>Mitochondries</term>
<term>Noyau de la cellule</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is an emerging infectious disease. Its etiological agent has been convincingly identified as a new member of family Coronaviridae (SARS-CoV). It causes serious damage to the respiratory system yet the mechanism is not clear. Infection-induced apoptosis or necrosis is suspected but no direct evidence for this yet exists. To date, Vero E6 cells are the only cell line that could be used to replicate the virus with obvious CPE (cytopathic effect) in vitro. It is known for some viruses (including members of family Coronaviridae) that CPE can be caused either by virus-induced apoptosis (active death) or cell necrosis (passive death). In this study, we examined the apoptosis in the SARS-CoV infected Vero E6 cells. Indeed, the results do show that the CPE was induced by apoptosis rather than necrosis, shown by typical DNA fragmentation, through the existence of apoptotic bodies and swollen mitochondria. This observation has some implications for the SARS-CoV pathogenicity: SARS-CoV does induce apoptosis in cell cultures and might have the same effect in vivo, responsible for the severe damage of the respiratory system.</div>
</front>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
<region><li>Hubei</li>
</region>
<settlement><li>Wuhan</li>
</settlement>
<orgName><li>Université de Wuhan</li>
</orgName>
</list>
<tree><noCountry><name sortKey="Cole, David K" sort="Cole, David K" uniqKey="Cole D" first="David K" last="Cole">David K. Cole</name>
<name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F" last="Gao">George F. Gao</name>
<name sortKey="Hu, Yuanyang" sort="Hu, Yuanyang" uniqKey="Hu Y" first="Yuanyang" last="Hu">Yuanyang Hu</name>
<name sortKey="Xiao, Gengfu" sort="Xiao, Gengfu" uniqKey="Xiao G" first="Gengfu" last="Xiao">Gengfu Xiao</name>
<name sortKey="Yuan, Fang" sort="Yuan, Fang" uniqKey="Yuan F" first="Fang" last="Yuan">Fang Yuan</name>
<name sortKey="Zhang, Jiamin" sort="Zhang, Jiamin" uniqKey="Zhang J" first="Jiamin" last="Zhang">Jiamin Zhang</name>
<name sortKey="Zheng, Congyi" sort="Zheng, Congyi" uniqKey="Zheng C" first="Congyi" last="Zheng">Congyi Zheng</name>
</noCountry>
<country name="République populaire de Chine"><region name="Hubei"><name sortKey="Yan, Huimin" sort="Yan, Huimin" uniqKey="Yan H" first="Huimin" last="Yan">Huimin Yan</name>
</region>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005131 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 005131 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Main |étape= Exploration |type= RBID |clé= pubmed:15170624 |texte= SARS coronavirus induces apoptosis in Vero E6 cells. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:15170624" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
This area was generated with Dilib version V0.6.33. |