SARS coronavirus induces apoptosis in Vero E6 cells.
Identifieur interne : 000838 ( Ncbi/Merge ); précédent : 000837; suivant : 000839SARS coronavirus induces apoptosis in Vero E6 cells.
Auteurs : Huimin Yan [République populaire de Chine] ; Gengfu Xiao ; Jiamin Zhang ; Yuanyang Hu ; Fang Yuan ; David K. Cole ; Congyi Zheng ; George F. GaoSource :
- Journal of medical virology [ 0146-6615 ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux, Apoptose, Cellules Vero, Chromatine (ultrastructure), Coloration et marquage, Effet cytopathogène viral, Fluorescence, Fragmentation de l'ADN, Immunochimie, Microscopie électronique, Mitochondries (anatomopathologie), Mitochondries (ultrastructure), Noyau de la cellule (anatomopathologie), Noyau de la cellule (ultrastructure), Virus du SRAS (croissance et développement), Virus du SRAS (pathogénicité).
- MESH :
- anatomopathologie : Mitochondries, Noyau de la cellule.
- croissance et développement : Virus du SRAS.
- pathogénicité : Virus du SRAS.
- ultrastructure : Animaux, Apoptose, Cellules Vero, Chromatine, Coloration et marquage, Effet cytopathogène viral, Fluorescence, Fragmentation de l'ADN, Immunochimie, Microscopie électronique, Mitochondries, Noyau de la cellule.
English descriptors
- KwdEn :
- Animals, Apoptosis, Cell Nucleus (pathology), Cell Nucleus (ultrastructure), Chlorocebus aethiops, Chromatin (ultrastructure), Cytopathogenic Effect, Viral, DNA Fragmentation, Fluorescence, Immunochemistry, Microscopy, Electron, Mitochondria (pathology), Mitochondria (ultrastructure), SARS Virus (growth & development), SARS Virus (pathogenicity), Staining and Labeling, Vero Cells.
- MESH :
- chemical , ultrastructure : Chromatin.
- growth & development : SARS Virus.
- pathogenicity : SARS Virus.
- pathology : Cell Nucleus, Mitochondria.
- ultrastructure : Cell Nucleus, Mitochondria.
- Animals, Apoptosis, Chlorocebus aethiops, Cytopathogenic Effect, Viral, DNA Fragmentation, Fluorescence, Immunochemistry, Microscopy, Electron, Staining and Labeling, Vero Cells.
Abstract
Severe acute respiratory syndrome (SARS) is an emerging infectious disease. Its etiological agent has been convincingly identified as a new member of family Coronaviridae (SARS-CoV). It causes serious damage to the respiratory system yet the mechanism is not clear. Infection-induced apoptosis or necrosis is suspected but no direct evidence for this yet exists. To date, Vero E6 cells are the only cell line that could be used to replicate the virus with obvious CPE (cytopathic effect) in vitro. It is known for some viruses (including members of family Coronaviridae) that CPE can be caused either by virus-induced apoptosis (active death) or cell necrosis (passive death). In this study, we examined the apoptosis in the SARS-CoV infected Vero E6 cells. Indeed, the results do show that the CPE was induced by apoptosis rather than necrosis, shown by typical DNA fragmentation, through the existence of apoptotic bodies and swollen mitochondria. This observation has some implications for the SARS-CoV pathogenicity: SARS-CoV does induce apoptosis in cell cultures and might have the same effect in vivo, responsible for the severe damage of the respiratory system.
DOI: 10.1002/jmv.20094
PubMed: 15170624
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Gao</name></author></analytic><series><title level="j">Journal of medical virology</title><idno type="ISSN">0146-6615</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Apoptosis</term><term>Cell Nucleus (pathology)</term><term>Cell Nucleus (ultrastructure)</term><term>Chlorocebus aethiops</term><term>Chromatin (ultrastructure)</term><term>Cytopathogenic Effect, Viral</term><term>DNA Fragmentation</term><term>Fluorescence</term><term>Immunochemistry</term><term>Microscopy, Electron</term><term>Mitochondria (pathology)</term><term>Mitochondria (ultrastructure)</term><term>SARS Virus (growth & development)</term><term>SARS Virus (pathogenicity)</term><term>Staining and Labeling</term><term>Vero Cells</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Apoptose</term><term>Cellules Vero</term><term>Chromatine (ultrastructure)</term><term>Coloration et marquage</term><term>Effet cytopathogène viral</term><term>Fluorescence</term><term>Fragmentation de l'ADN</term><term>Immunochimie</term><term>Microscopie électronique</term><term>Mitochondries (anatomopathologie)</term><term>Mitochondries (ultrastructure)</term><term>Noyau de la cellule (anatomopathologie)</term><term>Noyau de la cellule (ultrastructure)</term><term>Virus du SRAS (croissance et développement)</term><term>Virus du SRAS (pathogénicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="ultrastructure" xml:lang="en"><term>Chromatin</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Mitochondries</term><term>Noyau de la cellule</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Cell Nucleus</term><term>Mitochondria</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Cell Nucleus</term><term>Mitochondria</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Apoptosis</term><term>Chlorocebus aethiops</term><term>Cytopathogenic Effect, Viral</term><term>DNA Fragmentation</term><term>Fluorescence</term><term>Immunochemistry</term><term>Microscopy, Electron</term><term>Staining and Labeling</term><term>Vero Cells</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Animaux</term><term>Apoptose</term><term>Cellules Vero</term><term>Chromatine</term><term>Coloration et marquage</term><term>Effet cytopathogène viral</term><term>Fluorescence</term><term>Fragmentation de l'ADN</term><term>Immunochimie</term><term>Microscopie électronique</term><term>Mitochondries</term><term>Noyau de la cellule</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is an emerging infectious disease. Its etiological agent has been convincingly identified as a new member of family Coronaviridae (SARS-CoV). It causes serious damage to the respiratory system yet the mechanism is not clear. Infection-induced apoptosis or necrosis is suspected but no direct evidence for this yet exists. To date, Vero E6 cells are the only cell line that could be used to replicate the virus with obvious CPE (cytopathic effect) in vitro. It is known for some viruses (including members of family Coronaviridae) that CPE can be caused either by virus-induced apoptosis (active death) or cell necrosis (passive death). In this study, we examined the apoptosis in the SARS-CoV infected Vero E6 cells. Indeed, the results do show that the CPE was induced by apoptosis rather than necrosis, shown by typical DNA fragmentation, through the existence of apoptotic bodies and swollen mitochondria. This observation has some implications for the SARS-CoV pathogenicity: SARS-CoV does induce apoptosis in cell cultures and might have the same effect in vivo, responsible for the severe damage of the respiratory system.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15170624</PMID><DateCompleted><Year>2004</Year><Month>12</Month><Day>21</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0146-6615</ISSN><JournalIssue CitedMedium="Print"><Volume>73</Volume><Issue>3</Issue><PubDate><Year>2004</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Journal of medical virology</Title><ISOAbbreviation>J. Med. Virol.</ISOAbbreviation></Journal><ArticleTitle>SARS coronavirus induces apoptosis in Vero E6 cells.</ArticleTitle><Pagination><MedlinePgn>323-31</MedlinePgn></Pagination><Abstract><AbstractText>Severe acute respiratory syndrome (SARS) is an emerging infectious disease. Its etiological agent has been convincingly identified as a new member of family Coronaviridae (SARS-CoV). It causes serious damage to the respiratory system yet the mechanism is not clear. Infection-induced apoptosis or necrosis is suspected but no direct evidence for this yet exists. To date, Vero E6 cells are the only cell line that could be used to replicate the virus with obvious CPE (cytopathic effect) in vitro. It is known for some viruses (including members of family Coronaviridae) that CPE can be caused either by virus-induced apoptosis (active death) or cell necrosis (passive death). In this study, we examined the apoptosis in the SARS-CoV infected Vero E6 cells. Indeed, the results do show that the CPE was induced by apoptosis rather than necrosis, shown by typical DNA fragmentation, through the existence of apoptotic bodies and swollen mitochondria. This observation has some implications for the SARS-CoV pathogenicity: SARS-CoV does induce apoptosis in cell cultures and might have the same effect in vivo, responsible for the severe damage of the respiratory system.</AbstractText><CopyrightInformation>Copyright 2004 Wiley-Liss, Inc.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yan</LastName><ForeName>Huimin</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>School of Life Sciences, Wuhan University, Wuhan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xiao</LastName><ForeName>Gengfu</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Jiamin</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Yuanyang</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Yuan</LastName><ForeName>Fang</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Cole</LastName><ForeName>David K</ForeName><Initials>DK</Initials></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Congyi</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Gao</LastName><ForeName>George F</ForeName><Initials>GF</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Med Virol</MedlineTA><NlmUniqueID>7705876</NlmUniqueID><ISSNLinking>0146-6615</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002843">Chromatin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017209" MajorTopicYN="Y">Apoptosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002467" MajorTopicYN="N">Cell Nucleus</DescriptorName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002843" MajorTopicYN="N">Chromatin</DescriptorName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003588" MajorTopicYN="Y">Cytopathogenic Effect, Viral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053938" MajorTopicYN="Y">DNA Fragmentation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005453" MajorTopicYN="N">Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007120" MajorTopicYN="N">Immunochemistry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008854" MajorTopicYN="N">Microscopy, Electron</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008928" MajorTopicYN="N">Mitochondria</DescriptorName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013194" MajorTopicYN="N">Staining and Labeling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>6</Month><Day>2</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>12</Month><Day>22</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>6</Month><Day>2</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15170624</ArticleId><ArticleId IdType="doi">10.1002/jmv.20094</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Hubei</li></region><settlement><li>Wuhan</li></settlement><orgName><li>Université de Wuhan</li></orgName></list><tree><noCountry><name sortKey="Cole, David K" sort="Cole, David K" uniqKey="Cole D" first="David K" last="Cole">David K. Cole</name><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F" last="Gao">George F. Gao</name><name sortKey="Hu, Yuanyang" sort="Hu, Yuanyang" uniqKey="Hu Y" first="Yuanyang" last="Hu">Yuanyang Hu</name><name sortKey="Xiao, Gengfu" sort="Xiao, Gengfu" uniqKey="Xiao G" first="Gengfu" last="Xiao">Gengfu Xiao</name><name sortKey="Yuan, Fang" sort="Yuan, Fang" uniqKey="Yuan F" first="Fang" last="Yuan">Fang Yuan</name><name sortKey="Zhang, Jiamin" sort="Zhang, Jiamin" uniqKey="Zhang J" first="Jiamin" last="Zhang">Jiamin Zhang</name><name sortKey="Zheng, Congyi" sort="Zheng, Congyi" uniqKey="Zheng C" first="Congyi" last="Zheng">Congyi Zheng</name></noCountry><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Yan, Huimin" sort="Yan, Huimin" uniqKey="Yan H" first="Huimin" last="Yan">Huimin Yan</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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