SrasV1, Main, Exploration, bibRecord, 004E43

Development and evaluation of a multitarget real-time Taqman reverse transcription-PCR assay for detection of the severe acute respiratory syndrome-associated coronavirus and surveillance for an apparently related coronavirus found in masked palm civets

Identifieur interne : 004E43 ( Main/Exploration ); précédent : 004E42; suivant : 004E44

Development and evaluation of a multitarget real-time Taqman reverse transcription-PCR assay for detection of the severe acute respiratory syndrome-associated coronavirus and surveillance for an apparently related coronavirus found in masked palm civets

Auteurs : WENQIAN HU [République populaire de Chine] ; BINGKE BAI [République populaire de Chine] ; ZHIHONG HU [République populaire de Chine] ; ZE CHEN [République populaire de Chine] ; XUEFANG AN [République populaire de Chine] ; LIJUN TANG [République populaire de Chine] ; JIHONG YANG [République populaire de Chine] ; HUALIN WANG [République populaire de Chine] ; HANZHONG WANG [République populaire de Chine]

Source :

Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2005.

RBID : Pascal:05-0241571

Descripteurs français

Pascal (Inist)
- Coronavirus, Temps réel, Réaction chaîne polymérase RT, Détection, Microbiologie, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Coronavirus, Detection, Microbiology, Real time, Reverse transcription polymerase chain reaction, Severe acute respiratory syndrome.

Abstract

Severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) is the etiological agent of SARS. It is believed that SARS-CoV originates from wild animals. We have developed a multitarget real-time Taqman reverse transcription-PCR (RT-PCR) assay for the quantitative detection of SARS-CoV. The sequences of the Taqman probes with a minor groove binder and the corresponding primers were based on the sequences of the N gene, open reading frame (ORF) 3, and ORF 8. The overall linear range of this assay was from at least 10¹ to 10⁶ copies per reaction, and the detection limit could reach less than 10 copies per reaction. The quantification results for SARS-CoV from cell culture correlated well with those of the RT-PCR by using any two of the three sets of primer and probe used in this assay. However, the results of quantification of SARS-CoV obtained by using a few available throat swab specimens from SARS patients and the N gene as the target were almost 10 times higher than those obtained by using ORF 3 and ORF 8. Using this assay, we also detected an apparently SARS-CoV-related coronavirus in the throat swab specimens from masked palm civets in the west part of Hubei Province, People's Republic of China.

Affiliations:

République populaire de Chine

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Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term>
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<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) is the etiological agent of SARS. It is believed that SARS-CoV originates from wild animals. We have developed a multitarget real-time Taqman reverse transcription-PCR (RT-PCR) assay for the quantitative detection of SARS-CoV. The sequences of the Taqman probes with a minor groove binder and the corresponding primers were based on the sequences of the N gene, open reading frame (ORF) 3, and ORF 8. The overall linear range of this assay was from at least 10<sup>1</sup>
 to 10<sup>6</sup>
 copies per reaction, and the detection limit could reach less than 10 copies per reaction. The quantification results for SARS-CoV from cell culture correlated well with those of the RT-PCR by using any two of the three sets of primer and probe used in this assay. However, the results of quantification of SARS-CoV obtained by using a few available throat swab specimens from SARS patients and the N gene as the target were almost 10 times higher than those obtained by using ORF 3 and ORF 8. Using this assay, we also detected an apparently SARS-CoV-related coronavirus in the throat swab specimens from masked palm civets in the west part of Hubei Province, People's Republic of China.</div>
</front>
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<name sortKey="Xuefang An" sort="Xuefang An" uniqKey="Xuefang An" last="Xuefang An">XUEFANG AN</name>
<name sortKey="Ze Chen" sort="Ze Chen" uniqKey="Ze Chen" last="Ze Chen">ZE CHEN</name>
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Development and evaluation of a multitarget real-time Taqman reverse transcription-PCR assay for detection of the severe acute respiratory syndrome-associated coronavirus and surveillance for an apparently related coronavirus found in masked palm civets

Development and evaluation of a multitarget real-time Taqman reverse transcription-PCR assay for detection of the severe acute respiratory syndrome-associated coronavirus and surveillance for an apparently related coronavirus found in masked palm civets

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri