Development and evaluation of a multitarget real-time Taqman reverse transcription-PCR assay for detection of the severe acute respiratory syndrome-associated coronavirus and surveillance for an apparently related coronavirus found in masked palm civets
Identifieur interne : 000671 ( PascalFrancis/Checkpoint ); précédent : 000670; suivant : 000672Development and evaluation of a multitarget real-time Taqman reverse transcription-PCR assay for detection of the severe acute respiratory syndrome-associated coronavirus and surveillance for an apparently related coronavirus found in masked palm civets
Auteurs : WENQIAN HU [République populaire de Chine] ; BINGKE BAI [République populaire de Chine] ; ZHIHONG HU [République populaire de Chine] ; ZE CHEN [République populaire de Chine] ; XUEFANG AN [République populaire de Chine] ; LIJUN TANG [République populaire de Chine] ; JIHONG YANG [République populaire de Chine] ; HUALIN WANG [République populaire de Chine] ; HANZHONG WANG [République populaire de Chine]Source :
- Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2005.
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Abstract
Severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) is the etiological agent of SARS. It is believed that SARS-CoV originates from wild animals. We have developed a multitarget real-time Taqman reverse transcription-PCR (RT-PCR) assay for the quantitative detection of SARS-CoV. The sequences of the Taqman probes with a minor groove binder and the corresponding primers were based on the sequences of the N gene, open reading frame (ORF) 3, and ORF 8. The overall linear range of this assay was from at least 101 to 106 copies per reaction, and the detection limit could reach less than 10 copies per reaction. The quantification results for SARS-CoV from cell culture correlated well with those of the RT-PCR by using any two of the three sets of primer and probe used in this assay. However, the results of quantification of SARS-CoV obtained by using a few available throat swab specimens from SARS patients and the N gene as the target were almost 10 times higher than those obtained by using ORF 3 and ORF 8. Using this assay, we also detected an apparently SARS-CoV-related coronavirus in the throat swab specimens from masked palm civets in the west part of Hubei Province, People's Republic of China.
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Development and evaluation of a multitarget real-time Taqman reverse transcription-PCR assay for detection of the severe acute respiratory syndrome-associated coronavirus and surveillance for an apparently related coronavirus found in masked palm civets</title><author><name sortKey="Wenqian Hu" sort="Wenqian Hu" uniqKey="Wenqian Hu" last="Wenqian Hu">WENQIAN HU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan, Hubei</wicri:noRegion></affiliation></author><author><name sortKey="Bingke Bai" sort="Bingke Bai" uniqKey="Bingke Bai" last="Bingke Bai">BINGKE BAI</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan, Hubei</wicri:noRegion></affiliation></author><author><name sortKey="Zhihong Hu" sort="Zhihong Hu" uniqKey="Zhihong Hu" last="Zhihong Hu">ZHIHONG HU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan, Hubei</wicri:noRegion></affiliation></author><author><name sortKey="Ze Chen" sort="Ze Chen" uniqKey="Ze Chen" last="Ze Chen">ZE CHEN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan, Hubei</wicri:noRegion></affiliation></author><author><name sortKey="Xuefang An" sort="Xuefang An" uniqKey="Xuefang An" last="Xuefang An">XUEFANG AN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan, Hubei</wicri:noRegion></affiliation></author><author><name sortKey="Lijun Tang" sort="Lijun Tang" uniqKey="Lijun Tang" last="Lijun Tang">LIJUN TANG</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Hubei Center for Diseases Control and Prevention</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>6 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hubei Center for Diseases Control and Prevention</wicri:noRegion></affiliation></author><author><name sortKey="Jihong Yang" sort="Jihong Yang" uniqKey="Jihong Yang" last="Jihong Yang">JIHONG YANG</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Wuhan Center for Diseases Control and Prevention</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan Center for Diseases Control and Prevention</wicri:noRegion></affiliation></author><author><name sortKey="Hualin Wang" sort="Hualin Wang" uniqKey="Hualin Wang" last="Hualin Wang">HUALIN WANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan, Hubei</wicri:noRegion></affiliation></author><author><name sortKey="Hanzhong Wang" sort="Hanzhong Wang" uniqKey="Hanzhong Wang" last="Hanzhong Wang">HANZHONG WANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan, Hubei</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">05-0241571</idno><date when="2005">2005</date><idno type="stanalyst">PASCAL 05-0241571 INIST</idno><idno type="RBID">Pascal:05-0241571</idno><idno type="wicri:Area/PascalFrancis/Corpus">000679</idno><idno type="wicri:Area/PascalFrancis/Curation">000311</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000671</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000671</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Development and evaluation of a multitarget real-time Taqman reverse transcription-PCR assay for detection of the severe acute respiratory syndrome-associated coronavirus and surveillance for an apparently related coronavirus found in masked palm civets</title><author><name sortKey="Wenqian Hu" sort="Wenqian Hu" uniqKey="Wenqian Hu" last="Wenqian Hu">WENQIAN HU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan, Hubei</wicri:noRegion></affiliation></author><author><name sortKey="Bingke Bai" sort="Bingke Bai" uniqKey="Bingke Bai" last="Bingke Bai">BINGKE BAI</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan, Hubei</wicri:noRegion></affiliation></author><author><name sortKey="Zhihong Hu" sort="Zhihong Hu" uniqKey="Zhihong Hu" last="Zhihong Hu">ZHIHONG HU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan, Hubei</wicri:noRegion></affiliation></author><author><name sortKey="Ze Chen" sort="Ze Chen" uniqKey="Ze Chen" last="Ze Chen">ZE CHEN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan, Hubei</wicri:noRegion></affiliation></author><author><name sortKey="Xuefang An" sort="Xuefang An" uniqKey="Xuefang An" last="Xuefang An">XUEFANG AN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan, Hubei</wicri:noRegion></affiliation></author><author><name sortKey="Lijun Tang" sort="Lijun Tang" uniqKey="Lijun Tang" last="Lijun Tang">LIJUN TANG</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Hubei Center for Diseases Control and Prevention</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>6 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hubei Center for Diseases Control and Prevention</wicri:noRegion></affiliation></author><author><name sortKey="Jihong Yang" sort="Jihong Yang" uniqKey="Jihong Yang" last="Jihong Yang">JIHONG YANG</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Wuhan Center for Diseases Control and Prevention</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan Center for Diseases Control and Prevention</wicri:noRegion></affiliation></author><author><name sortKey="Hualin Wang" sort="Hualin Wang" uniqKey="Hualin Wang" last="Hualin Wang">HUALIN WANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan, Hubei</wicri:noRegion></affiliation></author><author><name sortKey="Hanzhong Wang" sort="Hanzhong Wang" uniqKey="Hanzhong Wang" last="Hanzhong Wang">HANZHONG WANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan, Hubei</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of clinical microbiology : (Print)</title><title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title><idno type="ISSN">0095-1137</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of clinical microbiology : (Print)</title><title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title><idno type="ISSN">0095-1137</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term><term>Detection</term><term>Microbiology</term><term>Real time</term><term>Reverse transcription polymerase chain reaction</term><term>Severe acute respiratory syndrome</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term><term>Temps réel</term><term>Réaction chaîne polymérase RT</term><term>Détection</term><term>Microbiologie</term><term>Syndrome respiratoire aigu sévère</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) is the etiological agent of SARS. It is believed that SARS-CoV originates from wild animals. We have developed a multitarget real-time Taqman reverse transcription-PCR (RT-PCR) assay for the quantitative detection of SARS-CoV. The sequences of the Taqman probes with a minor groove binder and the corresponding primers were based on the sequences of the N gene, open reading frame (ORF) 3, and ORF 8. The overall linear range of this assay was from at least 10<sup>1</sup> to 10<sup>6</sup> copies per reaction, and the detection limit could reach less than 10 copies per reaction. The quantification results for SARS-CoV from cell culture correlated well with those of the RT-PCR by using any two of the three sets of primer and probe used in this assay. However, the results of quantification of SARS-CoV obtained by using a few available throat swab specimens from SARS patients and the N gene as the target were almost 10 times higher than those obtained by using ORF 3 and ORF 8. Using this assay, we also detected an apparently SARS-CoV-related coronavirus in the throat swab specimens from masked palm civets in the west part of Hubei Province, People's Republic of China.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0095-1137</s0></fA01><fA02 i1="01"><s0>JCMIDW</s0></fA02><fA03 i2="1"><s0>J. clin. microbiol. : (Print)</s0></fA03><fA05><s2>43</s2></fA05><fA06><s2>5</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Development and evaluation of a multitarget real-time Taqman reverse transcription-PCR assay for detection of the severe acute respiratory syndrome-associated coronavirus and surveillance for an apparently related coronavirus found in masked palm civets</s1></fA08><fA11 i1="01" i2="1"><s1>WENQIAN HU</s1></fA11><fA11 i1="02" i2="1"><s1>BINGKE BAI</s1></fA11><fA11 i1="03" i2="1"><s1>ZHIHONG HU</s1></fA11><fA11 i1="04" i2="1"><s1>ZE CHEN</s1></fA11><fA11 i1="05" i2="1"><s1>XUEFANG AN</s1></fA11><fA11 i1="06" i2="1"><s1>LIJUN TANG</s1></fA11><fA11 i1="07" i2="1"><s1>JIHONG YANG</s1></fA11><fA11 i1="08" i2="1"><s1>HUALIN WANG</s1></fA11><fA11 i1="09" i2="1"><s1>HANZHONG WANG</s1></fA11><fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></fA14><fA14 i1="02"><s1>Hubei Center for Diseases Control and Prevention</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>6 aut.</sZ></fA14><fA14 i1="03"><s1>Wuhan Center for Diseases Control and Prevention</s1><s2>Wuhan, Hubei</s2><s3>CHN</s3><sZ>7 aut.</sZ></fA14><fA20><s1>2041-2046</s1></fA20><fA21><s1>2005</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>17088</s2><s5>354000129821540010</s5></fA43><fA44><s0>0000</s0><s1>© 2005 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>23 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>05-0241571</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Journal of clinical microbiology : (Print)</s0></fA64><fA66 i1="01"><s0>USA</s0></fA66><fC01 i1="01" l="ENG"><s0>Severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) is the etiological agent of SARS. It is believed that SARS-CoV originates from wild animals. We have developed a multitarget real-time Taqman reverse transcription-PCR (RT-PCR) assay for the quantitative detection of SARS-CoV. The sequences of the Taqman probes with a minor groove binder and the corresponding primers were based on the sequences of the N gene, open reading frame (ORF) 3, and ORF 8. The overall linear range of this assay was from at least 10<sup>1</sup> to 10<sup>6</sup> copies per reaction, and the detection limit could reach less than 10 copies per reaction. The quantification results for SARS-CoV from cell culture correlated well with those of the RT-PCR by using any two of the three sets of primer and probe used in this assay. However, the results of quantification of SARS-CoV obtained by using a few available throat swab specimens from SARS patients and the N gene as the target were almost 10 times higher than those obtained by using ORF 3 and ORF 8. Using this assay, we also detected an apparently SARS-CoV-related coronavirus in the throat swab specimens from masked palm civets in the west part of Hubei Province, People's Republic of China.</s0></fC01><fC02 i1="01" i2="X"><s0>002A05C10</s0></fC02><fC02 i1="02" i2="X"><s0>002B05</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Coronavirus</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Coronavirus</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Coronavirus</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Temps réel</s0><s5>05</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Real time</s0><s5>05</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Tiempo real</s0><s5>05</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Réaction chaîne polymérase RT</s0><s5>06</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Reverse transcription polymerase chain reaction</s0><s5>06</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Reacción cadena polimerasa transcripción inversa</s0><s5>06</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Détection</s0><s5>07</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Detection</s0><s5>07</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>Detección</s0><s5>07</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>Microbiologie</s0><s5>08</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>Microbiology</s0><s5>08</s5></fC03><fC03 i1="05" i2="X" l="SPA"><s0>Microbiología</s0><s5>08</s5></fC03><fC03 i1="06" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0><s2>NM</s2><s5>14</s5></fC03><fC03 i1="06" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0><s2>NM</s2><s5>14</s5></fC03><fC03 i1="06" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0><s2>NM</s2><s5>14</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="FRE"><s0>Appareil respiratoire pathologie</s0><s5>13</s5></fC07><fC07 i1="04" i2="X" l="ENG"><s0>Respiratory disease</s0><s5>13</s5></fC07><fC07 i1="04" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0><s5>13</s5></fC07><fC07 i1="05" i2="X" l="FRE"><s0>Virose</s0></fC07><fC07 i1="05" i2="X" l="ENG"><s0>Viral disease</s0></fC07><fC07 i1="05" i2="X" l="SPA"><s0>Virosis</s0></fC07><fC07 i1="06" i2="X" l="FRE"><s0>Infection</s0></fC07><fC07 i1="06" i2="X" l="ENG"><s0>Infection</s0></fC07><fC07 i1="06" i2="X" l="SPA"><s0>Infección</s0></fC07><fC07 i1="07" i2="X" l="FRE"><s0>Poumon pathologie</s0><s5>16</s5></fC07><fC07 i1="07" i2="X" l="ENG"><s0>Lung disease</s0><s5>16</s5></fC07><fC07 i1="07" i2="X" l="SPA"><s0>Pulmón patología</s0><s5>16</s5></fC07><fN21><s1>164</s1></fN21><fN44 i1="01"><s1>OTO</s1></fN44><fN82><s1>OTO</s1></fN82></pA></standard></inist><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wenqian Hu" sort="Wenqian Hu" uniqKey="Wenqian Hu" last="Wenqian Hu">WENQIAN HU</name></noRegion><name sortKey="Bingke Bai" sort="Bingke Bai" uniqKey="Bingke Bai" last="Bingke Bai">BINGKE BAI</name><name sortKey="Hanzhong Wang" sort="Hanzhong Wang" uniqKey="Hanzhong Wang" last="Hanzhong Wang">HANZHONG WANG</name><name sortKey="Hualin Wang" sort="Hualin Wang" uniqKey="Hualin Wang" last="Hualin Wang">HUALIN WANG</name><name sortKey="Jihong Yang" sort="Jihong Yang" uniqKey="Jihong Yang" last="Jihong Yang">JIHONG YANG</name><name sortKey="Lijun Tang" sort="Lijun Tang" uniqKey="Lijun Tang" last="Lijun Tang">LIJUN TANG</name><name sortKey="Xuefang An" sort="Xuefang An" uniqKey="Xuefang An" last="Xuefang An">XUEFANG AN</name><name sortKey="Ze Chen" sort="Ze Chen" uniqKey="Ze Chen" last="Ze Chen">ZE CHEN</name><name sortKey="Zhihong Hu" sort="Zhihong Hu" uniqKey="Zhihong Hu" last="Zhihong Hu">ZHIHONG HU</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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