Establishment of Vero E6 cell clones persistently infected with severe acute respiratory syndrome coronavirus
Identifieur interne : 004E31 ( Main/Exploration ); précédent : 004E30; suivant : 004E32Establishment of Vero E6 cell clones persistently infected with severe acute respiratory syndrome coronavirus
Auteurs : Masanobu Yamate [Japon] ; Makiko Yamashita [Japon] ; Toshiyuki Goto [Japon] ; Shoutaro Tsuji [Japon] ; Yong-Gang Li [Japon] ; Jiranan Warachit [Japon] ; Mikihiro Yunoki [Japon] ; Kazuyoshi Ikuta [Japon]Source :
- Microbes and infection [ 1286-4579 ] ; 2005.
Descripteurs français
- KwdFr :
- ARN viral (analyse), Animaux, Antigènes de surface (analyse), Carboxypeptidases (analyse), Cellules Vero (virologie), Cytométrie en flux, Effet cytopathogène viral, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (analyse), Microscopie confocale, Microscopie de fluorescence, Microscopie électronique, Peptidyl-Dipeptidase A, Protéines de l'enveloppe virale (analyse), Protéines nucléocapside (analyse), Régulation négative, Technique de Western, Virus du SRAS (croissance et développement).
- MESH :
- analyse : ARN viral, Antigènes de surface, Carboxypeptidases, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines nucléocapside.
- croissance et développement : Virus du SRAS.
- virologie : Cellules Vero.
- Pascal (Inist)
- Animaux, Coronavirus, Cellule infectée, Cytométrie en flux, Effet cytopathogène viral, Glycoprotéine de spicule des coronavirus, Microscopie confocale, Microscopie de fluorescence, Microscopie électronique, Nucléocapside, Peptidyl-Dipeptidase A, Protéine, Infection persistante, Régulation négative, Syndrome respiratoire aigu sévère, Technique de Western.
English descriptors
- KwdEn :
- Animals, Antigens, Surface (analysis), Blotting, Western, Carboxypeptidases (analysis), Chlorocebus aethiops, Coronavirus, Cytopathogenic Effect, Viral, Down-Regulation, Flow Cytometry, Infected cell, Membrane Glycoproteins (analysis), Microscopy, Confocal, Microscopy, Electron, Microscopy, Fluorescence, Nucleocapsid, Nucleocapsid Proteins (analysis), Peptidyl-Dipeptidase A, Persistent infection, Protein, RNA, Viral (analysis), SARS Virus (growth & development), Severe acute respiratory syndrome, Spike Glycoprotein, Coronavirus, Vero Cells (virology), Viral Envelope Proteins (analysis).
- MESH :
- chemical , analysis : Antigens, Surface, Carboxypeptidases, Membrane Glycoproteins, Nucleocapsid Proteins, RNA, Viral, Viral Envelope Proteins.
- growth & development : SARS Virus.
- virology : Vero Cells.
- Animals, Blotting, Western, Chlorocebus aethiops, Cytopathogenic Effect, Viral, Down-Regulation, Flow Cytometry, Microscopy, Confocal, Microscopy, Electron, Microscopy, Fluorescence, Peptidyl-Dipeptidase A, Spike Glycoprotein, Coronavirus.
Abstract
Little information is available on persistent infection of severe acute respiratory syndrome (SARS) coronavirus (CoV). In this study, we established persistent infection of SARS-CoV in the Vero E6 cell line. Acute infection of Vero E6 with SARS-CoV produced a lytic infection with characteristic rounding cytopathic effects (CPE) and the production of a large number of infectious particles in the culture fluid within 3 days post-infection. Upon subsequent culturing of the remaining adherent cells, the cells gradually proliferated and recovered normal morphology similar to that of the parental cells, and continued to produce large numbers of infectious viral particles during the observation period of 5 months. Among a total of 87 cell clones obtained from the persistently infected Vero E6, only four cell clones (named #13, #18, #21, and #34) were positive for viral RNA. Clones #13, #18, and #34 shifted to viral RNA-negative during subsequent cultures, while #21 continuously produced infectious particles at a high rate. The SARS-CoV receptor, angiotensin-converting enzyme 2, was almost completely down regulated from the cell surface of persistently infected cells. Western blot analysis as well as electron microscopy indicated that the ratios of spike to nucleocapsid protein in clone #21 as well as its parental persistently infected cells were lower than that in the cells in the acute phase of infection. These Vero E6 cells persistently infected with SARS-CoV may be useful for clarifying the mechanism of the persistent infection and also for elucidating the possible pathophysiologic significance of such long-term maintenance of this virus.
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000502
- to stream PascalFrancis, to step Curation: 000488
- to stream PascalFrancis, to step Checkpoint: 000657
- to stream Main, to step Merge: 005177
- to stream PubMed, to step Corpus: 002460
- to stream PubMed, to step Curation: 002460
- to stream PubMed, to step Checkpoint: 002727
- to stream Ncbi, to step Merge: 001249
- to stream Ncbi, to step Curation: 001249
- to stream Ncbi, to step Checkpoint: 001249
- to stream Main, to step Merge: 004B42
- to stream Main, to step Curation: 004E31
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Establishment of Vero E6 cell clones persistently infected with severe acute respiratory syndrome coronavirus</title><author><name sortKey="Yamate, Masanobu" sort="Yamate, Masanobu" uniqKey="Yamate M" first="Masanobu" last="Yamate">Masanobu Yamate</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Research Institute for Microbial Diseases, Osaka University</s1><s2>Suita, Osaka 565-0871</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Suita, Osaka 565-0871</wicri:noRegion></affiliation></author><author><name sortKey="Yamashita, Makiko" sort="Yamashita, Makiko" uniqKey="Yamashita M" first="Makiko" last="Yamashita">Makiko Yamashita</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Research Institute for Microbial Diseases, Osaka University</s1><s2>Suita, Osaka 565-0871</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Suita, Osaka 565-0871</wicri:noRegion></affiliation></author><author><name sortKey="Goto, Toshiyuki" sort="Goto, Toshiyuki" uniqKey="Goto T" first="Toshiyuki" last="Goto">Toshiyuki Goto</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>School of Health Science, Faculty of Medicine, Kyoto University</s1><s2>Sakyo-ku, Kyoto 606-8507</s2><s3>JPN</s3><sZ>3 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName><orgName type="university">Université de Kyoto</orgName></affiliation></author><author><name sortKey="Tsuji, Shoutaro" sort="Tsuji, Shoutaro" uniqKey="Tsuji S" first="Shoutaro" last="Tsuji">Shoutaro Tsuji</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Research Institute for Microbial Diseases, Osaka University</s1><s2>Suita, Osaka 565-0871</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Suita, Osaka 565-0871</wicri:noRegion></affiliation></author><author><name sortKey="Li, Yong Gang" sort="Li, Yong Gang" uniqKey="Li Y" first="Yong-Gang" last="Li">Yong-Gang Li</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Research Institute for Microbial Diseases, Osaka University</s1><s2>Suita, Osaka 565-0871</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Suita, Osaka 565-0871</wicri:noRegion></affiliation></author><author><name sortKey="Warachit, Jiranan" sort="Warachit, Jiranan" uniqKey="Warachit J" first="Jiranan" last="Warachit">Jiranan Warachit</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Research Institute for Microbial Diseases, Osaka University</s1><s2>Suita, Osaka 565-0871</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Suita, Osaka 565-0871</wicri:noRegion></affiliation></author><author><name sortKey="Yunoki, Mikihiro" sort="Yunoki, Mikihiro" uniqKey="Yunoki M" first="Mikihiro" last="Yunoki">Mikihiro Yunoki</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Research Institute for Microbial Diseases, Osaka University</s1><s2>Suita, Osaka 565-0871</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Suita, Osaka 565-0871</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Research and Development Division, Benesis Corporation, 2-25-1, Shodai-Ohtani</s1><s2>Hirakata, Osaka 573-1153</s2><s3>JPN</s3><sZ>7 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Hirakata, Osaka 573-1153</wicri:noRegion></affiliation></author><author><name sortKey="Ikuta, Kazuyoshi" sort="Ikuta, Kazuyoshi" uniqKey="Ikuta K" first="Kazuyoshi" last="Ikuta">Kazuyoshi Ikuta</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Research Institute for Microbial Diseases, Osaka University</s1><s2>Suita, Osaka 565-0871</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Suita, Osaka 565-0871</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">06-0256876</idno><date when="2005">2005</date><idno type="stanalyst">PASCAL 06-0256876 INIST</idno><idno type="RBID">Pascal:06-0256876</idno><idno type="wicri:Area/PascalFrancis/Corpus">000502</idno><idno type="wicri:Area/PascalFrancis/Curation">000488</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000657</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000657</idno><idno type="wicri:doubleKey">1286-4579:2005:Yamate M:establishment:of:vero</idno><idno type="wicri:Area/Main/Merge">005177</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:16269264</idno><idno type="wicri:Area/PubMed/Corpus">002460</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002460</idno><idno type="wicri:Area/PubMed/Curation">002460</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002460</idno><idno type="wicri:Area/PubMed/Checkpoint">002727</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002727</idno><idno type="wicri:Area/Ncbi/Merge">001249</idno><idno type="wicri:Area/Ncbi/Curation">001249</idno><idno type="wicri:Area/Ncbi/Checkpoint">001249</idno><idno type="wicri:doubleKey">1286-4579:2005:Yamate M:establishment:of:vero</idno><idno type="wicri:Area/Main/Merge">004B42</idno><idno type="wicri:Area/Main/Curation">004E31</idno><idno type="wicri:Area/Main/Exploration">004E31</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Establishment of Vero E6 cell clones persistently infected with severe acute respiratory syndrome coronavirus</title><author><name sortKey="Yamate, Masanobu" sort="Yamate, Masanobu" uniqKey="Yamate M" first="Masanobu" last="Yamate">Masanobu Yamate</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Research Institute for Microbial Diseases, Osaka University</s1><s2>Suita, Osaka 565-0871</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Suita, Osaka 565-0871</wicri:noRegion></affiliation></author><author><name sortKey="Yamashita, Makiko" sort="Yamashita, Makiko" uniqKey="Yamashita M" first="Makiko" last="Yamashita">Makiko Yamashita</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Research Institute for Microbial Diseases, Osaka University</s1><s2>Suita, Osaka 565-0871</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Suita, Osaka 565-0871</wicri:noRegion></affiliation></author><author><name sortKey="Goto, Toshiyuki" sort="Goto, Toshiyuki" uniqKey="Goto T" first="Toshiyuki" last="Goto">Toshiyuki Goto</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>School of Health Science, Faculty of Medicine, Kyoto University</s1><s2>Sakyo-ku, Kyoto 606-8507</s2><s3>JPN</s3><sZ>3 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName><orgName type="university">Université de Kyoto</orgName></affiliation></author><author><name sortKey="Tsuji, Shoutaro" sort="Tsuji, Shoutaro" uniqKey="Tsuji S" first="Shoutaro" last="Tsuji">Shoutaro Tsuji</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Research Institute for Microbial Diseases, Osaka University</s1><s2>Suita, Osaka 565-0871</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Suita, Osaka 565-0871</wicri:noRegion></affiliation></author><author><name sortKey="Li, Yong Gang" sort="Li, Yong Gang" uniqKey="Li Y" first="Yong-Gang" last="Li">Yong-Gang Li</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Research Institute for Microbial Diseases, Osaka University</s1><s2>Suita, Osaka 565-0871</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Suita, Osaka 565-0871</wicri:noRegion></affiliation></author><author><name sortKey="Warachit, Jiranan" sort="Warachit, Jiranan" uniqKey="Warachit J" first="Jiranan" last="Warachit">Jiranan Warachit</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Research Institute for Microbial Diseases, Osaka University</s1><s2>Suita, Osaka 565-0871</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Suita, Osaka 565-0871</wicri:noRegion></affiliation></author><author><name sortKey="Yunoki, Mikihiro" sort="Yunoki, Mikihiro" uniqKey="Yunoki M" first="Mikihiro" last="Yunoki">Mikihiro Yunoki</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Research Institute for Microbial Diseases, Osaka University</s1><s2>Suita, Osaka 565-0871</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Suita, Osaka 565-0871</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Research and Development Division, Benesis Corporation, 2-25-1, Shodai-Ohtani</s1><s2>Hirakata, Osaka 573-1153</s2><s3>JPN</s3><sZ>7 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Hirakata, Osaka 573-1153</wicri:noRegion></affiliation></author><author><name sortKey="Ikuta, Kazuyoshi" sort="Ikuta, Kazuyoshi" uniqKey="Ikuta K" first="Kazuyoshi" last="Ikuta">Kazuyoshi Ikuta</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Research Institute for Microbial Diseases, Osaka University</s1><s2>Suita, Osaka 565-0871</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Suita, Osaka 565-0871</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Microbes and infection</title><title level="j" type="abbreviated">Microbes infect.</title><idno type="ISSN">1286-4579</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Microbes and infection</title><title level="j" type="abbreviated">Microbes infect.</title><idno type="ISSN">1286-4579</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antigens, Surface (analysis)</term><term>Blotting, Western</term><term>Carboxypeptidases (analysis)</term><term>Chlorocebus aethiops</term><term>Coronavirus</term><term>Cytopathogenic Effect, Viral</term><term>Down-Regulation</term><term>Flow Cytometry</term><term>Infected cell</term><term>Membrane Glycoproteins (analysis)</term><term>Microscopy, Confocal</term><term>Microscopy, Electron</term><term>Microscopy, Fluorescence</term><term>Nucleocapsid</term><term>Nucleocapsid Proteins (analysis)</term><term>Peptidyl-Dipeptidase A</term><term>Persistent infection</term><term>Protein</term><term>RNA, Viral (analysis)</term><term>SARS Virus (growth & development)</term><term>Severe acute respiratory syndrome</term><term>Spike Glycoprotein, Coronavirus</term><term>Vero Cells (virology)</term><term>Viral Envelope Proteins (analysis)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (analyse)</term><term>Animaux</term><term>Antigènes de surface (analyse)</term><term>Carboxypeptidases (analyse)</term><term>Cellules Vero (virologie)</term><term>Cytométrie en flux</term><term>Effet cytopathogène viral</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (analyse)</term><term>Microscopie confocale</term><term>Microscopie de fluorescence</term><term>Microscopie électronique</term><term>Peptidyl-Dipeptidase A</term><term>Protéines de l'enveloppe virale (analyse)</term><term>Protéines nucléocapside (analyse)</term><term>Régulation négative</term><term>Technique de Western</term><term>Virus du SRAS (croissance et développement)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antigens, Surface</term><term>Carboxypeptidases</term><term>Membrane Glycoproteins</term><term>Nucleocapsid Proteins</term><term>RNA, Viral</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term><term>Antigènes de surface</term><term>Carboxypeptidases</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines nucléocapside</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Cellules Vero</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Chlorocebus aethiops</term><term>Cytopathogenic Effect, Viral</term><term>Down-Regulation</term><term>Flow Cytometry</term><term>Microscopy, Confocal</term><term>Microscopy, Electron</term><term>Microscopy, Fluorescence</term><term>Peptidyl-Dipeptidase A</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Animaux</term><term>Coronavirus</term><term>Cellule infectée</term><term>Cytométrie en flux</term><term>Effet cytopathogène viral</term><term>Glycoprotéine de spicule des coronavirus</term><term>Microscopie confocale</term><term>Microscopie de fluorescence</term><term>Microscopie électronique</term><term>Nucléocapside</term><term>Peptidyl-Dipeptidase A</term><term>Protéine</term><term>Infection persistante</term><term>Régulation négative</term><term>Syndrome respiratoire aigu sévère</term><term>Technique de Western</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Little information is available on persistent infection of severe acute respiratory syndrome (SARS) coronavirus (CoV). In this study, we established persistent infection of SARS-CoV in the Vero E6 cell line. Acute infection of Vero E6 with SARS-CoV produced a lytic infection with characteristic rounding cytopathic effects (CPE) and the production of a large number of infectious particles in the culture fluid within 3 days post-infection. Upon subsequent culturing of the remaining adherent cells, the cells gradually proliferated and recovered normal morphology similar to that of the parental cells, and continued to produce large numbers of infectious viral particles during the observation period of 5 months. Among a total of 87 cell clones obtained from the persistently infected Vero E6, only four cell clones (named #13, #18, #21, and #34) were positive for viral RNA. Clones #13, #18, and #34 shifted to viral RNA-negative during subsequent cultures, while #21 continuously produced infectious particles at a high rate. The SARS-CoV receptor, angiotensin-converting enzyme 2, was almost completely down regulated from the cell surface of persistently infected cells. Western blot analysis as well as electron microscopy indicated that the ratios of spike to nucleocapsid protein in clone #21 as well as its parental persistently infected cells were lower than that in the cells in the acute phase of infection. These Vero E6 cells persistently infected with SARS-CoV may be useful for clarifying the mechanism of the persistent infection and also for elucidating the possible pathophysiologic significance of such long-term maintenance of this virus.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région du Kansai</li></region><settlement><li>Kyoto</li></settlement><orgName><li>Université de Kyoto</li></orgName></list><tree><country name="Japon"><noRegion><name sortKey="Yamate, Masanobu" sort="Yamate, Masanobu" uniqKey="Yamate M" first="Masanobu" last="Yamate">Masanobu Yamate</name></noRegion><name sortKey="Goto, Toshiyuki" sort="Goto, Toshiyuki" uniqKey="Goto T" first="Toshiyuki" last="Goto">Toshiyuki Goto</name><name sortKey="Ikuta, Kazuyoshi" sort="Ikuta, Kazuyoshi" uniqKey="Ikuta K" first="Kazuyoshi" last="Ikuta">Kazuyoshi Ikuta</name><name sortKey="Li, Yong Gang" sort="Li, Yong Gang" uniqKey="Li Y" first="Yong-Gang" last="Li">Yong-Gang Li</name><name sortKey="Tsuji, Shoutaro" sort="Tsuji, Shoutaro" uniqKey="Tsuji S" first="Shoutaro" last="Tsuji">Shoutaro Tsuji</name><name sortKey="Warachit, Jiranan" sort="Warachit, Jiranan" uniqKey="Warachit J" first="Jiranan" last="Warachit">Jiranan Warachit</name><name sortKey="Yamashita, Makiko" sort="Yamashita, Makiko" uniqKey="Yamashita M" first="Makiko" last="Yamashita">Makiko Yamashita</name><name sortKey="Yunoki, Mikihiro" sort="Yunoki, Mikihiro" uniqKey="Yunoki M" first="Mikihiro" last="Yunoki">Mikihiro Yunoki</name><name sortKey="Yunoki, Mikihiro" sort="Yunoki, Mikihiro" uniqKey="Yunoki M" first="Mikihiro" last="Yunoki">Mikihiro Yunoki</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004E31 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004E31 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= Pascal:06-0256876
|texte= Establishment of Vero E6 cell clones persistently infected with severe acute respiratory syndrome coronavirus
}}
| This area was generated with Dilib version V0.6.33. |  |