An atypical RNA pseudoknot stimulator and an upstream attenuation signal for −1 ribosomal frameshifting of SARS coronavirus
Identifieur interne : 004C81 ( Main/Exploration ); précédent : 004C80; suivant : 004C82An atypical RNA pseudoknot stimulator and an upstream attenuation signal for −1 ribosomal frameshifting of SARS coronavirus
Auteurs : Mei-Chi Su ; Chung-Te Chang ; Chiu-Hui Chu [Taïwan] ; Ching-Hsiu Tsai [Taïwan] ; Kung-Yao Chang [Taïwan]Source :
- Nucleic Acids Research [ 0305-1048 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : RNA, Viral.
- genetics : SARS Virus.
- Base Sequence, Down-Regulation, Frameshifting, Ribosomal, Gene Expression Regulation, Viral, Molecular Sequence Data, Nucleic Acid Conformation, Regulatory Sequences, Ribonucleic Acid.
Abstract
The −1 ribosomal frameshifting requires the existence of an in cis RNA slippery sequence and is promoted by a downstream stimulator RNA. An atypical RNA pseudoknot with an extra stem formed by complementary sequences within loop 2 of an H-type pseudoknot is characterized in the severe acute respiratory syndrome coronavirus (SARS CoV) genome. This pseudoknot can serve as an efficient stimulator for −1 frameshifting in vitro. Mutational analysis of the extra stem suggests frameshift efficiency can be modulated via manipulation of the secondary structure within the loop 2 of an infectious bronchitis virus-type pseudoknot. More importantly, an upstream RNA sequence separated by a linker 5′ to the slippery site is also identified to be capable of modulating the −1 frameshift efficiency. RNA sequence containing this attenuation element can downregulate −1 frameshifting promoted by an atypical pseudoknot of SARS CoV and two other pseudoknot stimulators. Furthermore, frameshift efficiency can be reduced to half in the presence of the attenuation signal in vivo. Therefore, this in cis RNA attenuator represents a novel negative determinant of general importance for the regulation of −1 frameshift efficiency, and is thus a potential antiviral target.
Url:
DOI: 10.1093/nar/gki731
Affiliations:
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Tel: +886 4 22840468</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Nucleic Acids Research</title><title level="j" type="abbrev">Nucl. Acids Res.</title><idno type="ISSN">0305-1048</idno><idno type="eISSN">1362-4962</idno><imprint><publisher>Oxford University Press</publisher><date type="published">2005</date><biblScope unit="vol">33</biblScope><biblScope unit="issue">13</biblScope><biblScope unit="page" from="4265">4265</biblScope><biblScope unit="page" to="4275">4275</biblScope></imprint><idno type="ISSN">0305-1048</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0305-1048</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>Down-Regulation</term><term>Frameshifting, Ribosomal</term><term>Gene Expression Regulation, Viral</term><term>Molecular Sequence Data</term><term>Nucleic Acid Conformation</term><term>RNA, Viral (chemistry)</term><term>Regulatory Sequences, Ribonucleic Acid</term><term>SARS Virus (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral ()</term><term>Conformation d'acide nucléique</term><term>Données de séquences moléculaires</term><term>Décalage ribosomique du cadre de lecture</term><term>Régulation de l'expression des gènes viraux</term><term>Régulation négative</term><term>Séquence nucléotidique</term><term>Séquences régulatrices de l'acide ribonucléique</term><term>Virus du SRAS (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Down-Regulation</term><term>Frameshifting, Ribosomal</term><term>Gene Expression Regulation, Viral</term><term>Molecular Sequence Data</term><term>Nucleic Acid Conformation</term><term>Regulatory Sequences, Ribonucleic Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ARN viral</term><term>Conformation d'acide nucléique</term><term>Données de séquences moléculaires</term><term>Décalage ribosomique du cadre de lecture</term><term>Régulation de l'expression des gènes viraux</term><term>Régulation négative</term><term>Séquence nucléotidique</term><term>Séquences régulatrices de l'acide ribonucléique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The −1 ribosomal frameshifting requires the existence of an in cis RNA slippery sequence and is promoted by a downstream stimulator RNA. An atypical RNA pseudoknot with an extra stem formed by complementary sequences within loop 2 of an H-type pseudoknot is characterized in the severe acute respiratory syndrome coronavirus (SARS CoV) genome. This pseudoknot can serve as an efficient stimulator for −1 frameshifting in vitro. Mutational analysis of the extra stem suggests frameshift efficiency can be modulated via manipulation of the secondary structure within the loop 2 of an infectious bronchitis virus-type pseudoknot. More importantly, an upstream RNA sequence separated by a linker 5′ to the slippery site is also identified to be capable of modulating the −1 frameshift efficiency. RNA sequence containing this attenuation element can downregulate −1 frameshifting promoted by an atypical pseudoknot of SARS CoV and two other pseudoknot stimulators. Furthermore, frameshift efficiency can be reduced to half in the presence of the attenuation signal in vivo. Therefore, this in cis RNA attenuator represents a novel negative determinant of general importance for the regulation of −1 frameshift efficiency, and is thus a potential antiviral target.</div></front></TEI><affiliations><list><country><li>Taïwan</li></country></list><tree><noCountry><name sortKey="Chang, Chung Te" sort="Chang, Chung Te" uniqKey="Chang C" first="Chung-Te" last="Chang">Chung-Te Chang</name><name sortKey="Su, Mei Chi" sort="Su, Mei Chi" uniqKey="Su M" first="Mei-Chi" last="Su">Mei-Chi Su</name></noCountry><country name="Taïwan"><noRegion><name sortKey="Chu, Chiu Hui" sort="Chu, Chiu Hui" uniqKey="Chu C" first="Chiu-Hui" last="Chu">Chiu-Hui Chu</name></noRegion><name sortKey="Chang, Kung Yao" sort="Chang, Kung Yao" uniqKey="Chang K" first="Kung-Yao" last="Chang">Kung-Yao Chang</name><name sortKey="Tsai, Ching Hsiu" sort="Tsai, Ching Hsiu" uniqKey="Tsai C" first="Ching-Hsiu" last="Tsai">Ching-Hsiu Tsai</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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