Papain-Like Protease 2 (PLP2) from Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV): Expression, Purification, Characterization, and Inhibition†
Identifieur interne : 004973 ( Main/Exploration ); précédent : 004972; suivant : 004974Papain-Like Protease 2 (PLP2) from Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV): Expression, Purification, Characterization, and Inhibition†
Auteurs : Yu-San Han [République populaire de Chine] ; Gu-Gang Chang [République populaire de Chine] ; Chiun-Gung Juo [République populaire de Chine] ; Hong-Jen Lee [République populaire de Chine] ; Shiou-Hwei Yeh [République populaire de Chine] ; John Tsu-An Hsu [République populaire de Chine] ; Xin Chen [République populaire de Chine, Taïwan]Source :
- Biochemistry [ 0006-2960 ] ; 2005.
Descripteurs français
- KwdFr :
- Catalyse, Cinétique, Concentration inhibitrice 50, Coronavirus bovin (enzymologie), Domaine catalytique, Données de séquences moléculaires, Hydrolyse, Inhibiteurs de la cystéine protéinase (pharmacologie), Inhibiteurs de la cystéine protéinase (synthèse chimique), Papaïne (antagonistes et inhibiteurs), Papaïne (biosynthèse), Papaïne (génétique), Papaïne (isolement et purification), Protéines virales (antagonistes et inhibiteurs), Protéines virales (biosynthèse), Protéines virales (génétique), Protéines virales (isolement et purification), Spécificité du substrat, Séquence d'acides aminés, Virus de l'hépatite murine (enzymologie), Virus du SRAS (enzymologie), Virus du SRAS (pathogénicité), Zinc (pharmacologie).
- MESH :
- antagonistes et inhibiteurs : Papaïne, Protéines virales.
- biosynthèse : Papaïne, Protéines virales.
- enzymologie : Coronavirus bovin, Virus de l'hépatite murine, Virus du SRAS.
- génétique : Papaïne, Protéines virales.
- isolement et purification : Papaïne, Protéines virales.
- pathogénicité : Virus du SRAS.
- pharmacologie : Inhibiteurs de la cystéine protéinase, Zinc.
- synthèse chimique : Inhibiteurs de la cystéine protéinase.
- Catalyse, Cinétique, Concentration inhibitrice 50, Domaine catalytique, Données de séquences moléculaires, Hydrolyse, Spécificité du substrat, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Catalysis, Catalytic Domain, Coronavirus, Bovine (enzymology), Cysteine Proteinase Inhibitors (chemical synthesis), Cysteine Proteinase Inhibitors (pharmacology), Hydrolysis, Inhibitory Concentration 50, Kinetics, Molecular Sequence Data, Murine hepatitis virus (enzymology), Papain (antagonists & inhibitors), Papain (biosynthesis), Papain (genetics), Papain (isolation & purification), SARS Virus (enzymology), SARS Virus (pathogenicity), Substrate Specificity, Viral Proteins (antagonists & inhibitors), Viral Proteins (biosynthesis), Viral Proteins (genetics), Viral Proteins (isolation & purification), Zinc (pharmacology).
- MESH :
- chemical , antagonists & inhibitors : Papain, Viral Proteins.
- chemical , biosynthesis : Papain, Viral Proteins.
- chemical , chemical synthesis : Cysteine Proteinase Inhibitors.
- enzymology : Coronavirus, Bovine, Murine hepatitis virus, SARS Virus.
- chemical , genetics : Papain, Viral Proteins.
- chemical , isolation & purification : Papain, Viral Proteins.
- pathogenicity : SARS Virus.
- chemical , pharmacology : Cysteine Proteinase Inhibitors, Zinc.
- Amino Acid Sequence, Catalysis, Catalytic Domain, Hydrolysis, Inhibitory Concentration 50, Kinetics, Molecular Sequence Data, Substrate Specificity.
Abstract
Viral proteases are essential for pathogenesis and virulence of severe acute respiratory syndrome coronavirus (SARS-CoV). Little information is available on SARS-CoV papain-like protease 2 (PLP2), and development of inhibitors against PLP2 is attractive for antiviral therapy. Here, we report the characterization of SARS-CoV PLP2 (from residues 1414 to 1858) purified from baculovirus-infected insect cells. We demonstrate that SARS-CoV PLP2 by itself differentially cleaves between the amino acids Gly180 and Ala181, Gly818 and Ala819, and Gly2740 and Lys2741 of the viral polypeptide pp1a, as determined by reversed-phase high-performance liquid chromatography analysis coupled with mass spectrometry. This protease is especially selective for the P1, P4, and P6 sites of the substrate. The study demonstrates, for the first time among coronaviral PLPs, that the reaction mechanism of SARS-CoV PLP2 is characteristic of papain and compatible with the involvement of the catalytic dyad (Cys)-S-/(His)-Im+H ion pair. With a fluorogenic inhibitor-screening platform, we show that zinc ion and its conjugates potently inhibit the enzymatic activity of SARS-CoV PLP2. In addition, we provided evidence for evolutionary reclassification of SARS-CoV. The results provide important insights into the biochemical properties of the coronaviral PLP family and a promising therapeutic way to fight SARS-CoV.
Url:
DOI: 10.1021/bi0504761
Affiliations:
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Le document en format XML
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Catalysis</term>
<term>Catalytic Domain</term>
<term>Coronavirus, Bovine (enzymology)</term>
<term>Cysteine Proteinase Inhibitors (chemical synthesis)</term>
<term>Cysteine Proteinase Inhibitors (pharmacology)</term>
<term>Hydrolysis</term>
<term>Inhibitory Concentration 50</term>
<term>Kinetics</term>
<term>Molecular Sequence Data</term>
<term>Murine hepatitis virus (enzymology)</term>
<term>Papain (antagonists & inhibitors)</term>
<term>Papain (biosynthesis)</term>
<term>Papain (genetics)</term>
<term>Papain (isolation & purification)</term>
<term>SARS Virus (enzymology)</term>
<term>SARS Virus (pathogenicity)</term>
<term>Substrate Specificity</term>
<term>Viral Proteins (antagonists & inhibitors)</term>
<term>Viral Proteins (biosynthesis)</term>
<term>Viral Proteins (genetics)</term>
<term>Viral Proteins (isolation & purification)</term>
<term>Zinc (pharmacology)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Catalyse</term>
<term>Cinétique</term>
<term>Concentration inhibitrice 50</term>
<term>Coronavirus bovin (enzymologie)</term>
<term>Domaine catalytique</term>
<term>Données de séquences moléculaires</term>
<term>Hydrolyse</term>
<term>Inhibiteurs de la cystéine protéinase (pharmacologie)</term>
<term>Inhibiteurs de la cystéine protéinase (synthèse chimique)</term>
<term>Papaïne (antagonistes et inhibiteurs)</term>
<term>Papaïne (biosynthèse)</term>
<term>Papaïne (génétique)</term>
<term>Papaïne (isolement et purification)</term>
<term>Protéines virales (antagonistes et inhibiteurs)</term>
<term>Protéines virales (biosynthèse)</term>
<term>Protéines virales (génétique)</term>
<term>Protéines virales (isolement et purification)</term>
<term>Spécificité du substrat</term>
<term>Séquence d'acides aminés</term>
<term>Virus de l'hépatite murine (enzymologie)</term>
<term>Virus du SRAS (enzymologie)</term>
<term>Virus du SRAS (pathogénicité)</term>
<term>Zinc (pharmacologie)</term>
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<keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Papain</term>
<term>Viral Proteins</term>
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<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Papain</term>
<term>Viral Proteins</term>
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<term>Protéines virales</term>
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<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Coronavirus bovin</term>
<term>Virus de l'hépatite murine</term>
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<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Coronavirus, Bovine</term>
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<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Papain</term>
<term>Viral Proteins</term>
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<term>Protéines virales</term>
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<term>Zinc</term>
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<term>Zinc</term>
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</keywords>
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<term>Catalysis</term>
<term>Catalytic Domain</term>
<term>Hydrolysis</term>
<term>Inhibitory Concentration 50</term>
<term>Kinetics</term>
<term>Molecular Sequence Data</term>
<term>Substrate Specificity</term>
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<term>Cinétique</term>
<term>Concentration inhibitrice 50</term>
<term>Domaine catalytique</term>
<term>Données de séquences moléculaires</term>
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<front><div type="abstract">Viral proteases are essential for pathogenesis and virulence of severe acute respiratory syndrome coronavirus (SARS-CoV). Little information is available on SARS-CoV papain-like protease 2 (PLP2), and development of inhibitors against PLP2 is attractive for antiviral therapy. Here, we report the characterization of SARS-CoV PLP2 (from residues 1414 to 1858) purified from baculovirus-infected insect cells. We demonstrate that SARS-CoV PLP2 by itself differentially cleaves between the amino acids Gly180 and Ala181, Gly818 and Ala819, and Gly2740 and Lys2741 of the viral polypeptide pp1a, as determined by reversed-phase high-performance liquid chromatography analysis coupled with mass spectrometry. This protease is especially selective for the P1, P4, and P6 sites of the substrate. The study demonstrates, for the first time among coronaviral PLPs, that the reaction mechanism of SARS-CoV PLP2 is characteristic of papain and compatible with the involvement of the catalytic dyad (Cys)-S-/(His)-Im+H ion pair. With a fluorogenic inhibitor-screening platform, we show that zinc ion and its conjugates potently inhibit the enzymatic activity of SARS-CoV PLP2. In addition, we provided evidence for evolutionary reclassification of SARS-CoV. The results provide important insights into the biochemical properties of the coronaviral PLP family and a promising therapeutic way to fight SARS-CoV.</div>
</front>
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<affiliations><list><country><li>République populaire de Chine</li>
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<name sortKey="Chang, Gu Gang" sort="Chang, Gu Gang" uniqKey="Chang G" first="Gu-Gang" last="Chang">Gu-Gang Chang</name>
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<name sortKey="Hsu, John Tsu An" sort="Hsu, John Tsu An" uniqKey="Hsu J" first="John Tsu-An" last="Hsu">John Tsu-An Hsu</name>
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