SrasV1, Main, Curation, bibRecord, 004973

Papain-Like Protease 2 (PLP2) from Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV): Expression, Purification, Characterization, and Inhibition†

Identifieur interne : 004973 ( Main/Curation ); précédent : 004972; suivant : 004974

Papain-Like Protease 2 (PLP2) from Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV): Expression, Purification, Characterization, and Inhibition†

Auteurs : Yu-San Han [République populaire de Chine] ; Gu-Gang Chang [République populaire de Chine] ; Chiun-Gung Juo [République populaire de Chine] ; Hong-Jen Lee [République populaire de Chine] ; Shiou-Hwei Yeh [République populaire de Chine] ; John Tsu-An Hsu [République populaire de Chine] ; Xin Chen [République populaire de Chine, Taïwan]

Source :

Biochemistry [ 0006-2960 ] ; 2005.

RBID : ISTEX:4F0E4AFE461DA666EE29EB3709FAED6D24EE468B

Descripteurs français

KwdFr :
- Catalyse, Cinétique, Concentration inhibitrice 50, Coronavirus bovin (enzymologie), Domaine catalytique, Données de séquences moléculaires, Hydrolyse, Inhibiteurs de la cystéine protéinase (pharmacologie), Inhibiteurs de la cystéine protéinase (synthèse chimique), Papaïne (antagonistes et inhibiteurs), Papaïne (biosynthèse), Papaïne (génétique), Papaïne (isolement et purification), Protéines virales (antagonistes et inhibiteurs), Protéines virales (biosynthèse), Protéines virales (génétique), Protéines virales (isolement et purification), Spécificité du substrat, Séquence d'acides aminés, Virus de l'hépatite murine (enzymologie), Virus du SRAS (enzymologie), Virus du SRAS (pathogénicité), Zinc (pharmacologie).
MESH :
- antagonistes et inhibiteurs : Papaïne, Protéines virales.
- biosynthèse : Papaïne, Protéines virales.
- enzymologie : Coronavirus bovin, Virus de l'hépatite murine, Virus du SRAS.
- génétique : Papaïne, Protéines virales.
- isolement et purification : Papaïne, Protéines virales.
- pathogénicité : Virus du SRAS.
- pharmacologie : Inhibiteurs de la cystéine protéinase, Zinc.
- synthèse chimique : Inhibiteurs de la cystéine protéinase.
- Catalyse, Cinétique, Concentration inhibitrice 50, Domaine catalytique, Données de séquences moléculaires, Hydrolyse, Spécificité du substrat, Séquence d'acides aminés.

English descriptors

KwdEn :
- Amino Acid Sequence, Catalysis, Catalytic Domain, Coronavirus, Bovine (enzymology), Cysteine Proteinase Inhibitors (chemical synthesis), Cysteine Proteinase Inhibitors (pharmacology), Hydrolysis, Inhibitory Concentration 50, Kinetics, Molecular Sequence Data, Murine hepatitis virus (enzymology), Papain (antagonists & inhibitors), Papain (biosynthesis), Papain (genetics), Papain (isolation & purification), SARS Virus (enzymology), SARS Virus (pathogenicity), Substrate Specificity, Viral Proteins (antagonists & inhibitors), Viral Proteins (biosynthesis), Viral Proteins (genetics), Viral Proteins (isolation & purification), Zinc (pharmacology).
MESH :
- chemical , antagonists & inhibitors : Papain, Viral Proteins.
- chemical , biosynthesis : Papain, Viral Proteins.
- chemical , chemical synthesis : Cysteine Proteinase Inhibitors.
- enzymology : Coronavirus, Bovine, Murine hepatitis virus, SARS Virus.
- chemical , genetics : Papain, Viral Proteins.
- chemical , isolation & purification : Papain, Viral Proteins.
- pathogenicity : SARS Virus.
- chemical , pharmacology : Cysteine Proteinase Inhibitors, Zinc.
- Amino Acid Sequence, Catalysis, Catalytic Domain, Hydrolysis, Inhibitory Concentration 50, Kinetics, Molecular Sequence Data, Substrate Specificity.

Abstract

Viral proteases are essential for pathogenesis and virulence of severe acute respiratory syndrome coronavirus (SARS-CoV). Little information is available on SARS-CoV papain-like protease 2 (PLP2), and development of inhibitors against PLP2 is attractive for antiviral therapy. Here, we report the characterization of SARS-CoV PLP2 (from residues 1414 to 1858) purified from baculovirus-infected insect cells. We demonstrate that SARS-CoV PLP2 by itself differentially cleaves between the amino acids Gly180 and Ala181, Gly818 and Ala819, and Gly2740 and Lys2741 of the viral polypeptide pp1a, as determined by reversed-phase high-performance liquid chromatography analysis coupled with mass spectrometry. This protease is especially selective for the P1, P4, and P6 sites of the substrate. The study demonstrates, for the first time among coronaviral PLPs, that the reaction mechanism of SARS-CoV PLP2 is characteristic of papain and compatible with the involvement of the catalytic dyad (Cys)-S-/(His)-Im+H ion pair. With a fluorogenic inhibitor-screening platform, we show that zinc ion and its conjugates potently inhibit the enzymatic activity of SARS-CoV PLP2. In addition, we provided evidence for evolutionary reclassification of SARS-CoV. The results provide important insights into the biochemical properties of the coronaviral PLP family and a promising therapeutic way to fight SARS-CoV.

Url:

https://api.istex.fr/ark:/67375/TPS-D5BW2ZKB-0/fulltext.pdf

DOI: 10.1021/bi0504761

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000024
to stream Istex, to step Curation: Pour aller vers cette notice dans l'étape Curation :000024
to stream Istex, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001834
to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002625
to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :002625
to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :002590
to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :001077
to stream Ncbi, to step Curation: Pour aller vers cette notice dans l'étape Curation :001077
to stream Ncbi, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001077
to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :004D01

Links to Exploration step

ISTEX:4F0E4AFE461DA666EE29EB3709FAED6D24EE468B

Le document en format XML

<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Papain-Like Protease 2 (PLP2) from Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV):  Expression, Purification, Characterization, and Inhibition†</title>
<author><name sortKey="Han, Yu San" sort="Han, Yu San" uniqKey="Han Y" first="Yu-San" last="Han">Yu-San Han</name>
</author>
<author><name sortKey="Chang, Gu Gang" sort="Chang, Gu Gang" uniqKey="Chang G" first="Gu-Gang" last="Chang">Gu-Gang Chang</name>
</author>
<author><name sortKey="Juo, Chiun Gung" sort="Juo, Chiun Gung" uniqKey="Juo C" first="Chiun-Gung" last="Juo">Chiun-Gung Juo</name>
</author>
<author><name sortKey="Lee, Hong Jen" sort="Lee, Hong Jen" uniqKey="Lee H" first="Hong-Jen" last="Lee">Hong-Jen Lee</name>
</author>
<author><name sortKey="Yeh, Shiou Hwei" sort="Yeh, Shiou Hwei" uniqKey="Yeh S" first="Shiou-Hwei" last="Yeh">Shiou-Hwei Yeh</name>
</author>
<author><name sortKey="Hsu, John Tsu An" sort="Hsu, John Tsu An" uniqKey="Hsu J" first="John Tsu-An" last="Hsu">John Tsu-An Hsu</name>
</author>
<author><name sortKey="Chen, Xin" sort="Chen, Xin" uniqKey="Chen X" first="Xin" last="Chen">Xin Chen</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:4F0E4AFE461DA666EE29EB3709FAED6D24EE468B</idno>
<date when="2005" year="2005">2005</date>
<idno type="doi">10.1021/bi0504761</idno>
<idno type="url">https://api.istex.fr/ark:/67375/TPS-D5BW2ZKB-0/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000024</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000024</idno>
<idno type="wicri:Area/Istex/Curation">000024</idno>
<idno type="wicri:Area/Istex/Checkpoint">001834</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001834</idno>
<idno type="wicri:doubleKey">0006-2960:2005:Han Y:papain:like:protease</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:16042412</idno>
<idno type="wicri:Area/PubMed/Corpus">002625</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002625</idno>
<idno type="wicri:Area/PubMed/Curation">002625</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002625</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002590</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002590</idno>
<idno type="wicri:Area/Ncbi/Merge">001077</idno>
<idno type="wicri:Area/Ncbi/Curation">001077</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">001077</idno>
<idno type="wicri:doubleKey">0006-2960:2005:Han Y:papain:like:protease</idno>
<idno type="wicri:Area/Main/Merge">004D01</idno>
<idno type="wicri:Area/Main/Curation">004973</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main">Papain-Like Protease 2 (PLP2) from Severe Acute Respiratory Syndrome
Coronavirus (SARS-CoV):  Expression, Purification, Characterization, and
Inhibition<ref type="bib" target="#bi0504761AF2">†</ref>
</title>
<author><name sortKey="Han, Yu San" sort="Han, Yu San" uniqKey="Han Y" first="Yu-San" last="Han">Yu-San Han</name>
<affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Division of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli County, Taiwan 350,Republic of China, Faculty of Life Science, National Yang-Ming University, Taipei, Taiwan 112, Republic of China, Institute ofBiomedical Sciences, Academia Sinica, Taipei, Taiwan 115, Republic of China, Division of Molecular and Genomic Medicine,National Health Research Institutes, Miaoli County, Taiwan 350</wicri:regionArea>
<wicri:noRegion>Taiwan 350</wicri:noRegion>
</affiliation>
<affiliation></affiliation>
</author>
<author><name sortKey="Chang, Gu Gang" sort="Chang, Gu Gang" uniqKey="Chang G" first="Gu-Gang" last="Chang">Gu-Gang Chang</name>
<affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Division of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli County, Taiwan 350,Republic of China, Faculty of Life Science, National Yang-Ming University, Taipei, Taiwan 112, Republic of China, Institute ofBiomedical Sciences, Academia Sinica, Taipei, Taiwan 115, Republic of China, Division of Molecular and Genomic Medicine,National Health Research Institutes, Miaoli County, Taiwan 350</wicri:regionArea>
<wicri:noRegion>Taiwan 350</wicri:noRegion>
</affiliation>
<affiliation></affiliation>
</author>
<author><name sortKey="Juo, Chiun Gung" sort="Juo, Chiun Gung" uniqKey="Juo C" first="Chiun-Gung" last="Juo">Chiun-Gung Juo</name>
<affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Division of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli County, Taiwan 350,Republic of China, Faculty of Life Science, National Yang-Ming University, Taipei, Taiwan 112, Republic of China, Institute ofBiomedical Sciences, Academia Sinica, Taipei, Taiwan 115, Republic of China, Division of Molecular and Genomic Medicine,National Health Research Institutes, Miaoli County, Taiwan 350</wicri:regionArea>
<wicri:noRegion>Taiwan 350</wicri:noRegion>
</affiliation>
<affiliation></affiliation>
</author>
<author><name sortKey="Lee, Hong Jen" sort="Lee, Hong Jen" uniqKey="Lee H" first="Hong-Jen" last="Lee">Hong-Jen Lee</name>
<affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Division of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli County, Taiwan 350,Republic of China, Faculty of Life Science, National Yang-Ming University, Taipei, Taiwan 112, Republic of China, Institute ofBiomedical Sciences, Academia Sinica, Taipei, Taiwan 115, Republic of China, Division of Molecular and Genomic Medicine,National Health Research Institutes, Miaoli County, Taiwan 350</wicri:regionArea>
<wicri:noRegion>Taiwan 350</wicri:noRegion>
</affiliation>
<affiliation></affiliation>
</author>
<author><name sortKey="Yeh, Shiou Hwei" sort="Yeh, Shiou Hwei" uniqKey="Yeh S" first="Shiou-Hwei" last="Yeh">Shiou-Hwei Yeh</name>
<affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Division of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli County, Taiwan 350,Republic of China, Faculty of Life Science, National Yang-Ming University, Taipei, Taiwan 112, Republic of China, Institute ofBiomedical Sciences, Academia Sinica, Taipei, Taiwan 115, Republic of China, Division of Molecular and Genomic Medicine,National Health Research Institutes, Miaoli County, Taiwan 350</wicri:regionArea>
<wicri:noRegion>Taiwan 350</wicri:noRegion>
</affiliation>
<affiliation></affiliation>
</author>
<author><name sortKey="Hsu, John Tsu An" sort="Hsu, John Tsu An" uniqKey="Hsu J" first="John Tsu-An" last="Hsu">John Tsu-An Hsu</name>
<affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Division of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli County, Taiwan 350,Republic of China, Faculty of Life Science, National Yang-Ming University, Taipei, Taiwan 112, Republic of China, Institute ofBiomedical Sciences, Academia Sinica, Taipei, Taiwan 115, Republic of China, Division of Molecular and Genomic Medicine,National Health Research Institutes, Miaoli County, Taiwan 350</wicri:regionArea>
<wicri:noRegion>Taiwan 350</wicri:noRegion>
</affiliation>
<affiliation></affiliation>
</author>
<author><name sortKey="Chen, Xin" sort="Chen, Xin" uniqKey="Chen X" first="Xin" last="Chen">Xin Chen</name>
<affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Division of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli County, Taiwan 350,Republic of China, Faculty of Life Science, National Yang-Ming University, Taipei, Taiwan 112, Republic of China, Institute ofBiomedical Sciences, Academia Sinica, Taipei, Taiwan 115, Republic of China, Division of Molecular and Genomic Medicine,National Health Research Institutes, Miaoli County, Taiwan 350</wicri:regionArea>
<wicri:noRegion>Taiwan 350</wicri:noRegion>
</affiliation>
<affiliation></affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">Taïwan</country>
<wicri:regionArea> To whom correspondence should be addressed:  Division of Biotechnology and Pharmaceutical Research, National Health ResearchInstitutes, Miaoli County, Taiwan 350</wicri:regionArea>
<wicri:noRegion>Taiwan 350</wicri:noRegion>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">Biochemistry</title>
<title level="j" type="abbrev">Biochemistry</title>
<idno type="ISSN">0006-2960</idno>
<idno type="eISSN">1520-4995</idno>
<imprint><publisher>American Chemical Society</publisher>
<date type="e-published">2005</date>
<date type="published">2005</date>
<biblScope unit="vol">44</biblScope>
<biblScope unit="issue">30</biblScope>
<biblScope unit="page" from="10349">10349</biblScope>
<biblScope unit="page" to="10359">10359</biblScope>
</imprint>
<idno type="ISSN">0006-2960</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0006-2960</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Catalysis</term>
<term>Catalytic Domain</term>
<term>Coronavirus, Bovine (enzymology)</term>
<term>Cysteine Proteinase Inhibitors (chemical synthesis)</term>
<term>Cysteine Proteinase Inhibitors (pharmacology)</term>
<term>Hydrolysis</term>
<term>Inhibitory Concentration 50</term>
<term>Kinetics</term>
<term>Molecular Sequence Data</term>
<term>Murine hepatitis virus (enzymology)</term>
<term>Papain (antagonists & inhibitors)</term>
<term>Papain (biosynthesis)</term>
<term>Papain (genetics)</term>
<term>Papain (isolation & purification)</term>
<term>SARS Virus (enzymology)</term>
<term>SARS Virus (pathogenicity)</term>
<term>Substrate Specificity</term>
<term>Viral Proteins (antagonists & inhibitors)</term>
<term>Viral Proteins (biosynthesis)</term>
<term>Viral Proteins (genetics)</term>
<term>Viral Proteins (isolation & purification)</term>
<term>Zinc (pharmacology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Catalyse</term>
<term>Cinétique</term>
<term>Concentration inhibitrice 50</term>
<term>Coronavirus bovin (enzymologie)</term>
<term>Domaine catalytique</term>
<term>Données de séquences moléculaires</term>
<term>Hydrolyse</term>
<term>Inhibiteurs de la cystéine protéinase (pharmacologie)</term>
<term>Inhibiteurs de la cystéine protéinase (synthèse chimique)</term>
<term>Papaïne (antagonistes et inhibiteurs)</term>
<term>Papaïne (biosynthèse)</term>
<term>Papaïne (génétique)</term>
<term>Papaïne (isolement et purification)</term>
<term>Protéines virales (antagonistes et inhibiteurs)</term>
<term>Protéines virales (biosynthèse)</term>
<term>Protéines virales (génétique)</term>
<term>Protéines virales (isolement et purification)</term>
<term>Spécificité du substrat</term>
<term>Séquence d'acides aminés</term>
<term>Virus de l'hépatite murine (enzymologie)</term>
<term>Virus du SRAS (enzymologie)</term>
<term>Virus du SRAS (pathogénicité)</term>
<term>Zinc (pharmacologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Papain</term>
<term>Viral Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Papain</term>
<term>Viral Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>Cysteine Proteinase Inhibitors</term>
</keywords>
<keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Papaïne</term>
<term>Protéines virales</term>
</keywords>
<keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Papaïne</term>
<term>Protéines virales</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Coronavirus bovin</term>
<term>Virus de l'hépatite murine</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Coronavirus, Bovine</term>
<term>Murine hepatitis virus</term>
<term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Papain</term>
<term>Viral Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Papaïne</term>
<term>Protéines virales</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Papain</term>
<term>Viral Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Papaïne</term>
<term>Protéines virales</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Inhibiteurs de la cystéine protéinase</term>
<term>Zinc</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Cysteine Proteinase Inhibitors</term>
<term>Zinc</term>
</keywords>
<keywords scheme="MESH" qualifier="synthèse chimique" xml:lang="fr"><term>Inhibiteurs de la cystéine protéinase</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Catalysis</term>
<term>Catalytic Domain</term>
<term>Hydrolysis</term>
<term>Inhibitory Concentration 50</term>
<term>Kinetics</term>
<term>Molecular Sequence Data</term>
<term>Substrate Specificity</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Catalyse</term>
<term>Cinétique</term>
<term>Concentration inhibitrice 50</term>
<term>Domaine catalytique</term>
<term>Données de séquences moléculaires</term>
<term>Hydrolyse</term>
<term>Spécificité du substrat</term>
<term>Séquence d'acides aminés</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract">Viral proteases are essential for pathogenesis and virulence of severe acute respiratory syndrome coronavirus (SARS-CoV). Little information is available on SARS-CoV papain-like protease 2 (PLP2), and development of inhibitors against PLP2 is attractive for antiviral therapy. Here, we report the characterization of SARS-CoV PLP2 (from residues 1414 to 1858) purified from baculovirus-infected insect cells. We demonstrate that SARS-CoV PLP2 by itself differentially cleaves between the amino acids Gly180 and Ala181, Gly818 and Ala819, and Gly2740 and Lys2741 of the viral polypeptide pp1a, as determined by reversed-phase high-performance liquid chromatography analysis coupled with mass spectrometry. This protease is especially selective for the P1, P4, and P6 sites of the substrate. The study demonstrates, for the first time among coronaviral PLPs, that the reaction mechanism of SARS-CoV PLP2 is characteristic of papain and compatible with the involvement of the catalytic dyad (Cys)-S-/(His)-Im+H ion pair. With a fluorogenic inhibitor-screening platform, we show that zinc ion and its conjugates potently inhibit the enzymatic activity of SARS-CoV PLP2. In addition, we provided evidence for evolutionary reclassification of SARS-CoV. The results provide important insights into the biochemical properties of the coronaviral PLP family and a promising therapeutic way to fight SARS-CoV.</div>
</front>
</TEI>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Curation

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004973 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 004973 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    Main
   |étape=   Curation
   |type=    RBID
   |clé=     ISTEX:4F0E4AFE461DA666EE29EB3709FAED6D24EE468B
   |texte=   Papain-Like Protease 2 (PLP2) from Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV):  Expression, Purification, Characterization, and Inhibition†
}}

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration SRAS

Papain-Like Protease 2 (PLP2) from Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV): Expression, Purification, Characterization, and Inhibition†

Papain-Like Protease 2 (PLP2) from Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV): Expression, Purification, Characterization, and Inhibition†

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri