Rapid viral diagnosis: role of electron microscopy.
Identifieur interne : 004687 ( Main/Exploration ); précédent : 004686; suivant : 004688Rapid viral diagnosis: role of electron microscopy.
Auteurs : Massimo Gentile [Italie] ; Hans R. GelderblomSource :
- The new microbiologica [ 1121-7138 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- diagnostic : Maladies virales.
- isolement et purification : Virus.
- ultrastructure : Coloration et marquage, Humains, Microscopie électronique, Prélèvement biologique, Prévision, Virus.
English descriptors
- KwdEn :
- MESH :
- diagnosis : Virus Diseases.
- isolation & purification : Viruses.
- ultrastructure : Viruses.
- Forecasting, Humans, Microscopy, Electron, Specimen Handling, Staining and Labeling.
Abstract
Starting in the 1960, electron microscopy (EM) became widely applied also in viral diagnosis. During the 1970th and 80th, many new agents were characterized from diagnostic cell cultures and clinical specimens. The wide introduction of ELISA- and PCR-techniques as well as cost-arguments recently reduced the role of EM in routine viral diagnosis. Compared to other diagnostic techniques, however, EM excells by speed and "open view", i.e. by the ability to detect also the "un-expected" without the need for specific reagents. As shown in 2003 by the elucidation of the SARS pandemia and the human monkeypox outbreak in US, EM is well suited as a safe, front-line diagnostic method in infectious diseases emergencies and/or in possible bioterrorist attacks.
PubMed: 15782621
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en">Starting in the 1960, electron microscopy (EM) became widely applied also in viral diagnosis. During the 1970th and 80th, many new agents were characterized from diagnostic cell cultures and clinical specimens. The wide introduction of ELISA- and PCR-techniques as well as cost-arguments recently reduced the role of EM in routine viral diagnosis. Compared to other diagnostic techniques, however, EM excells by speed and "open view", i.e. by the ability to detect also the "un-expected" without the need for specific reagents. As shown in 2003 by the elucidation of the SARS pandemia and the human monkeypox outbreak in US, EM is well suited as a safe, front-line diagnostic method in infectious diseases emergencies and/or in possible bioterrorist attacks.</div>
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