Rapid viral diagnosis: role of electron microscopy.
Identifieur interne : 002828 ( PubMed/Corpus ); précédent : 002827; suivant : 002829Rapid viral diagnosis: role of electron microscopy.
Auteurs : Massimo Gentile ; Hans R. GelderblomSource :
- The new microbiologica [ 1121-7138 ] ; 2005.
English descriptors
- KwdEn :
- MESH :
- diagnosis : Virus Diseases.
- isolation & purification : Viruses.
- ultrastructure : Viruses.
- Forecasting, Humans, Microscopy, Electron, Specimen Handling, Staining and Labeling.
Abstract
Starting in the 1960, electron microscopy (EM) became widely applied also in viral diagnosis. During the 1970th and 80th, many new agents were characterized from diagnostic cell cultures and clinical specimens. The wide introduction of ELISA- and PCR-techniques as well as cost-arguments recently reduced the role of EM in routine viral diagnosis. Compared to other diagnostic techniques, however, EM excells by speed and "open view", i.e. by the ability to detect also the "un-expected" without the need for specific reagents. As shown in 2003 by the elucidation of the SARS pandemia and the human monkeypox outbreak in US, EM is well suited as a safe, front-line diagnostic method in infectious diseases emergencies and/or in possible bioterrorist attacks.
PubMed: 15782621
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pubmed:15782621Le document en format XML
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Gelderblom</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15782621</idno><idno type="pmid">15782621</idno><idno type="wicri:Area/PubMed/Corpus">002828</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002828</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Rapid viral diagnosis: role of electron microscopy.</title><author><name sortKey="Gentile, Massimo" sort="Gentile, Massimo" uniqKey="Gentile M" first="Massimo" last="Gentile">Massimo Gentile</name><affiliation><nlm:affiliation>Department of Experimental Medicine and Pathology, Virology Section, University La Sapienza, Rome, Italy. Massimo.gentile@uniroma1.it</nlm:affiliation></affiliation></author><author><name sortKey="Gelderblom, Hans R" sort="Gelderblom, Hans R" uniqKey="Gelderblom H" first="Hans R" last="Gelderblom">Hans R. Gelderblom</name></author></analytic><series><title level="j">The new microbiologica</title><idno type="ISSN">1121-7138</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Forecasting</term><term>Humans</term><term>Microscopy, Electron</term><term>Specimen Handling</term><term>Staining and Labeling</term><term>Virus Diseases (diagnosis)</term><term>Viruses (isolation & purification)</term><term>Viruses (ultrastructure)</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Virus Diseases</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Viruses</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Viruses</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Forecasting</term><term>Humans</term><term>Microscopy, Electron</term><term>Specimen Handling</term><term>Staining and Labeling</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Starting in the 1960, electron microscopy (EM) became widely applied also in viral diagnosis. During the 1970th and 80th, many new agents were characterized from diagnostic cell cultures and clinical specimens. The wide introduction of ELISA- and PCR-techniques as well as cost-arguments recently reduced the role of EM in routine viral diagnosis. Compared to other diagnostic techniques, however, EM excells by speed and "open view", i.e. by the ability to detect also the "un-expected" without the need for specific reagents. As shown in 2003 by the elucidation of the SARS pandemia and the human monkeypox outbreak in US, EM is well suited as a safe, front-line diagnostic method in infectious diseases emergencies and/or in possible bioterrorist attacks.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15782621</PMID><DateCompleted><Year>2005</Year><Month>06</Month><Day>02</Day></DateCompleted><DateRevised><Year>2011</Year><Month>02</Month><Day>25</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1121-7138</ISSN><JournalIssue CitedMedium="Print"><Volume>28</Volume><Issue>1</Issue><PubDate><Year>2005</Year><Month>Jan</Month></PubDate></JournalIssue><Title>The new microbiologica</Title><ISOAbbreviation>New Microbiol.</ISOAbbreviation></Journal><ArticleTitle>Rapid viral diagnosis: role of electron microscopy.</ArticleTitle><Pagination><MedlinePgn>1-12</MedlinePgn></Pagination><Abstract><AbstractText>Starting in the 1960, electron microscopy (EM) became widely applied also in viral diagnosis. During the 1970th and 80th, many new agents were characterized from diagnostic cell cultures and clinical specimens. The wide introduction of ELISA- and PCR-techniques as well as cost-arguments recently reduced the role of EM in routine viral diagnosis. Compared to other diagnostic techniques, however, EM excells by speed and "open view", i.e. by the ability to detect also the "un-expected" without the need for specific reagents. As shown in 2003 by the elucidation of the SARS pandemia and the human monkeypox outbreak in US, EM is well suited as a safe, front-line diagnostic method in infectious diseases emergencies and/or in possible bioterrorist attacks.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gentile</LastName><ForeName>Massimo</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Experimental Medicine and Pathology, Virology Section, University La Sapienza, Rome, Italy. 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