Severe acute respiratory syndrome (SARS) S protein production in plants: Development of recombinant vaccine
Identifieur interne : 004639 ( Main/Exploration ); précédent : 004638; suivant : 004640Severe acute respiratory syndrome (SARS) S protein production in plants: Development of recombinant vaccine
Auteurs : Natalia Pogrebnyak ; Maxim Golovkin ; Vyacheslav Andrianov ; Sergei Spitsin ; Yuriy Smirnov ; Richard Egolf ; Hilary KoprowskiSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2005.
Descripteurs français
- KwdFr :
- ARN messager (génétique), Amorces ADN, Animaux, Cartographie de restriction, Clonage moléculaire, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Lycopersicon esculentum (virologie), Protéines de l'enveloppe virale (génétique), Protéines virales (génétique), RT-PCR, Réaction de polymérisation en chaîne, Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère (immunologie), Séquence d'acides aminés, Séquence nucléotidique, Vaccins antiviraux, Virus du SRAS (génétique), Virus du SRAS (immunologie), Végétaux génétiquement modifiés.
- MESH :
- génétique : ARN messager, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines virales, Virus du SRAS.
- immunologie : Syndrome respiratoire aigu sévère, Virus du SRAS.
- virologie : Lycopersicon esculentum.
- Amorces ADN, Animaux, Cartographie de restriction, Clonage moléculaire, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, RT-PCR, Réaction de polymérisation en chaîne, Souris, Souris de lignée BALB C, Séquence d'acides aminés, Séquence nucléotidique, Vaccins antiviraux, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, DNA Primers, Lycopersicon esculentum (virology), Membrane Glycoproteins (genetics), Mice, Mice, Inbred BALB C, Molecular Sequence Data, Plants, Genetically Modified, Polymerase Chain Reaction, RNA, Messenger (genetics), Restriction Mapping, Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (genetics), Viral Proteins (genetics), Viral Vaccines.
- MESH :
- chemical , genetics : Membrane Glycoproteins, RNA, Messenger, Viral Envelope Proteins, Viral Proteins.
- chemical : DNA Primers, Spike Glycoprotein, Coronavirus, Viral Vaccines.
- genetics : SARS Virus.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- virology : Lycopersicon esculentum.
- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Plants, Genetically Modified, Polymerase Chain Reaction, Restriction Mapping, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
In view of a recent spread of severe acute respiratory syndrome (SARS), there is a high demand for production of a vaccine to prevent this disease. Recent studies indicate that SARS-coronavirus (CoV) spike protein (S protein) and its truncated fragments are considered the best candidates for generation of the recombinant vaccine. Toward the development of a safe, effective, and inexpensive vaccine candidate, we have expressed the N-terminal fragment of SARS-CoV S protein (S1) in tomato and low-nicotine tobacco plants. Incorporation of the S1 fragment into plant genomes as well as its transcription was confirmed by PCR and RT-PCR analyses. High levels of expression of recombinant S1 protein were observed in several transgenic lines by Western blot analysis using specific antibodies. Plant-derived antigen was evaluated to induce the systemic and mucosal immune responses in mice. Mice showed significantly increased levels of SARS-CoV-specific IgA after oral ingestion of tomato fruits expressing S1 protein. Sera of mice parenterally primed with tobacco-derived S1 protein revealed the presence of SARS-CoV-specific IgG as detected by Western blot and ELISA analysis.
Url:
DOI: 10.1073/pnas.0503760102
PubMed: 15956182
PubMed Central: 1157057
Affiliations:
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Le document en format XML
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Egolf</name></author><author><name sortKey="Koprowski, Hilary" sort="Koprowski, Hilary" uniqKey="Koprowski H" first="Hilary" last="Koprowski">Hilary Koprowski</name></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><idno type="eISSN">1091-6490</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>DNA Primers</term><term>Lycopersicon esculentum (virology)</term><term>Membrane Glycoproteins (genetics)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Molecular Sequence Data</term><term>Plants, Genetically Modified</term><term>Polymerase Chain Reaction</term><term>RNA, Messenger (genetics)</term><term>Restriction Mapping</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Proteins (genetics)</term><term>Viral Vaccines</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (génétique)</term><term>Amorces ADN</term><term>Animaux</term><term>Cartographie de restriction</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Lycopersicon esculentum (virologie)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines virales (génétique)</term><term>RT-PCR</term><term>Réaction de polymérisation en chaîne</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Vaccins antiviraux</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>RNA, Messenger</term><term>Viral Envelope Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN messager</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines virales</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Lycopersicon esculentum</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Lycopersicon esculentum</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Molecular Sequence Data</term><term>Plants, Genetically Modified</term><term>Polymerase Chain Reaction</term><term>Restriction Mapping</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term><term>Animaux</term><term>Cartographie de restriction</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>RT-PCR</term><term>Réaction de polymérisation en chaîne</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Vaccins antiviraux</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>In view of a recent spread of severe acute respiratory syndrome (SARS), there is a high demand for production of a vaccine to prevent this disease. Recent studies indicate that SARS-coronavirus (CoV) spike protein (S protein) and its truncated fragments are considered the best candidates for generation of the recombinant vaccine. Toward the development of a safe, effective, and inexpensive vaccine candidate, we have expressed the N-terminal fragment of SARS-CoV S protein (S1) in tomato and low-nicotine tobacco plants. Incorporation of the S1 fragment into plant genomes as well as its transcription was confirmed by PCR and RT-PCR analyses. High levels of expression of recombinant S1 protein were observed in several transgenic lines by Western blot analysis using specific antibodies. Plant-derived antigen was evaluated to induce the systemic and mucosal immune responses in mice. Mice showed significantly increased levels of SARS-CoV-specific IgA after oral ingestion of tomato fruits expressing S1 protein. Sera of mice parenterally primed with tobacco-derived S1 protein revealed the presence of SARS-CoV-specific IgG as detected by Western blot and ELISA analysis.</p></div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Andrianov, Vyacheslav" sort="Andrianov, Vyacheslav" uniqKey="Andrianov V" first="Vyacheslav" last="Andrianov">Vyacheslav Andrianov</name><name sortKey="Egolf, Richard" sort="Egolf, Richard" uniqKey="Egolf R" first="Richard" last="Egolf">Richard Egolf</name><name sortKey="Golovkin, Maxim" sort="Golovkin, Maxim" uniqKey="Golovkin M" first="Maxim" last="Golovkin">Maxim Golovkin</name><name sortKey="Koprowski, Hilary" sort="Koprowski, Hilary" uniqKey="Koprowski H" first="Hilary" last="Koprowski">Hilary Koprowski</name><name sortKey="Pogrebnyak, Natalia" sort="Pogrebnyak, Natalia" uniqKey="Pogrebnyak N" first="Natalia" last="Pogrebnyak">Natalia Pogrebnyak</name><name sortKey="Smirnov, Yuriy" sort="Smirnov, Yuriy" uniqKey="Smirnov Y" first="Yuriy" last="Smirnov">Yuriy Smirnov</name><name sortKey="Spitsin, Sergei" sort="Spitsin, Sergei" uniqKey="Spitsin S" first="Sergei" last="Spitsin">Sergei Spitsin</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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