SrasV1, PubMed, Checkpoint, bibRecord, 002496

Severe acute respiratory syndrome (SARS) S protein production in plants: development of recombinant vaccine.

Identifieur interne : 002496 ( PubMed/Checkpoint ); précédent : 002495; suivant : 002497

Severe acute respiratory syndrome (SARS) S protein production in plants: development of recombinant vaccine.

Auteurs : Natalia Pogrebnyak [États-Unis] ; Maxim Golovkin ; Vyacheslav Andrianov ; Sergei Spitsin ; Yuriy Smirnov ; Richard Egolf ; Hilary Koprowski

Source :

Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2005.

RBID : pubmed:15956182

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, DNA Primers, Lycopersicon esculentum (virology), Membrane Glycoproteins (genetics), Mice, Mice, Inbred BALB C, Molecular Sequence Data, Plants, Genetically Modified, Polymerase Chain Reaction, RNA, Messenger (genetics), Restriction Mapping, Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (genetics), Viral Proteins (genetics), Viral Vaccines.
MESH :
- chemical , genetics : Membrane Glycoproteins, RNA, Messenger, Viral Envelope Proteins, Viral Proteins.
- chemical : DNA Primers, Spike Glycoprotein, Coronavirus, Viral Vaccines.
- genetics : SARS Virus.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- virology : Lycopersicon esculentum.
- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Plants, Genetically Modified, Polymerase Chain Reaction, Restriction Mapping, Reverse Transcriptase Polymerase Chain Reaction.

Abstract

In view of a recent spread of severe acute respiratory syndrome (SARS), there is a high demand for production of a vaccine to prevent this disease. Recent studies indicate that SARS-coronavirus (CoV) spike protein (S protein) and its truncated fragments are considered the best candidates for generation of the recombinant vaccine. Toward the development of a safe, effective, and inexpensive vaccine candidate, we have expressed the N-terminal fragment of SARS-CoV S protein (S1) in tomato and low-nicotine tobacco plants. Incorporation of the S1 fragment into plant genomes as well as its transcription was confirmed by PCR and RT-PCR analyses. High levels of expression of recombinant S1 protein were observed in several transgenic lines by Western blot analysis using specific antibodies. Plant-derived antigen was evaluated to induce the systemic and mucosal immune responses in mice. Mice showed significantly increased levels of SARS-CoV-specific IgA after oral ingestion of tomato fruits expressing S1 protein. Sera of mice parenterally primed with tobacco-derived S1 protein revealed the presence of SARS-CoV-specific IgG as detected by Western blot and ELISA analysis.

DOI: 10.1073/pnas.0503760102
PubMed: 15956182

Affiliations:

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Le document en format XML

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<front><div type="abstract" xml:lang="en">In view of a recent spread of severe acute respiratory syndrome (SARS), there is a high demand for production of a vaccine to prevent this disease. Recent studies indicate that SARS-coronavirus (CoV) spike protein (S protein) and its truncated fragments are considered the best candidates for generation of the recombinant vaccine. Toward the development of a safe, effective, and inexpensive vaccine candidate, we have expressed the N-terminal fragment of SARS-CoV S protein (S1) in tomato and low-nicotine tobacco plants. Incorporation of the S1 fragment into plant genomes as well as its transcription was confirmed by PCR and RT-PCR analyses. High levels of expression of recombinant S1 protein were observed in several transgenic lines by Western blot analysis using specific antibodies. Plant-derived antigen was evaluated to induce the systemic and mucosal immune responses in mice. Mice showed significantly increased levels of SARS-CoV-specific IgA after oral ingestion of tomato fruits expressing S1 protein. Sera of mice parenterally primed with tobacco-derived S1 protein revealed the presence of SARS-CoV-specific IgG as detected by Western blot and ELISA analysis.</div>
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Severe acute respiratory syndrome (SARS) S protein production in plants: development of recombinant vaccine.

Severe acute respiratory syndrome (SARS) S protein production in plants: development of recombinant vaccine.

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