A double-inactivated whole virus candidate SARS coronavirus vaccine stimulates neutralising and protective antibody responses
Identifieur interne : 004489 ( Main/Exploration ); précédent : 004488; suivant : 004490A double-inactivated whole virus candidate SARS coronavirus vaccine stimulates neutralising and protective antibody responses
Auteurs : Martin Spruth [Autriche] ; Otfried Kistner [Autriche] ; Helga Savidis-Dacho [Autriche] ; Elisabeth Hitter [Autriche] ; Brian Crowe [Autriche] ; Marijan Gerencer [Autriche] ; Peter Brühl [Autriche] ; Leopold Grillberger [Autriche] ; Manfred Reiter [Autriche] ; Christa Tauer [Autriche] ; Wolfgang Mundt [Autriche] ; P. Noel Barrett [Autriche]Source :
- Vaccine [ 0264-410X ] ; 2006.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Vaccin.
English descriptors
Abstract
A double-inactivated, candidate whole virus vaccine against severe acute respiratory syndrome associated coronavirus (SARS-CoV) was developed and manufactured at large scale using fermenter cultures of serum protein free Vero cells. A two step inactivation procedure involving sequential formaldehyde and U.V. inactivation was utilised in order to ensure an extremely high safety margin with respect to residual infectivity. The immunogenicity of this double-inactivated vaccine was characterised in the mouse model. Mice that were immunised twice with the candidate SARS-CoV vaccine developed high antibody titres against the SARS-CoV spike protein and high levels of neutralising antibodies. The use of the adjuvant Al(OH)3 had only a minor effect on the immunogenicity of the vaccine. In addition, cell mediated immunity as measured by interferon-γ and interleukin-4 stimulation, was elicited by vaccination. Moreover, the vaccine confers protective immunity as demonstrated by prevention of SARS-CoV replication in the respiratory tract of mice after intranasal challenge with SARS-CoV. Protection of mice was correlated to antibody titre against the SARS-CoV S protein and neutralising antibody titre.
Affiliations:
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type="wicri:doubleKey">0264-410X:2006:Spruth M:a:double:inactivated</idno><idno type="wicri:Area/Main/Merge">004721</idno><idno type="wicri:Area/Main/Curation">004489</idno><idno type="wicri:Area/Main/Exploration">004489</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">A double-inactivated whole virus candidate SARS coronavirus vaccine stimulates neutralising and protective antibody responses</title><author><name sortKey="Spruth, Martin" sort="Spruth, Martin" uniqKey="Spruth M" first="Martin" last="Spruth">Martin Spruth</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Baxter Vaccines, Biomedical Research Centre, Uferstr. 15</s1><s2>2304 Orth/Donau</s2><s3>AUT</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Autriche</country><wicri:noRegion>2304 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i1="01"><s1>Baxter Vaccines, Biomedical Research Centre, Uferstr. 15</s1><s2>2304 Orth/Donau</s2><s3>AUT</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Autriche</country><wicri:noRegion>2304 Orth/Donau</wicri:noRegion></affiliation></author><author><name sortKey="Gerencer, Marijan" sort="Gerencer, Marijan" uniqKey="Gerencer M" first="Marijan" last="Gerencer">Marijan Gerencer</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Baxter Vaccines, Biomedical Research Centre, Uferstr. 15</s1><s2>2304 Orth/Donau</s2><s3>AUT</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Autriche</country><wicri:noRegion>2304 Orth/Donau</wicri:noRegion></affiliation></author><author><name sortKey="Bruhl, Peter" sort="Bruhl, Peter" uniqKey="Bruhl P" first="Peter" last="Brühl">Peter Brühl</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Baxter Vaccines, Biomedical Research Centre, Uferstr. 15</s1><s2>2304 Orth/Donau</s2><s3>AUT</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Autriche</country><wicri:noRegion>2304 Orth/Donau</wicri:noRegion></affiliation></author><author><name sortKey="Grillberger, Leopold" sort="Grillberger, Leopold" uniqKey="Grillberger L" first="Leopold" last="Grillberger">Leopold Grillberger</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Baxter Vaccines, Biomedical Research Centre, Uferstr. 15</s1><s2>2304 Orth/Donau</s2><s3>AUT</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Autriche</country><wicri:noRegion>2304 Orth/Donau</wicri:noRegion></affiliation></author><author><name sortKey="Reiter, Manfred" sort="Reiter, Manfred" uniqKey="Reiter M" first="Manfred" last="Reiter">Manfred Reiter</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Baxter Vaccines, Biomedical Research Centre, Uferstr. 15</s1><s2>2304 Orth/Donau</s2><s3>AUT</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Autriche</country><wicri:noRegion>2304 Orth/Donau</wicri:noRegion></affiliation></author><author><name sortKey="Tauer, Christa" sort="Tauer, Christa" uniqKey="Tauer C" first="Christa" last="Tauer">Christa Tauer</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Baxter Vaccines, Biomedical Research Centre, Uferstr. 15</s1><s2>2304 Orth/Donau</s2><s3>AUT</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Autriche</country><wicri:noRegion>2304 Orth/Donau</wicri:noRegion></affiliation></author><author><name sortKey="Mundt, Wolfgang" sort="Mundt, Wolfgang" uniqKey="Mundt W" first="Wolfgang" last="Mundt">Wolfgang Mundt</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Baxter Vaccines, Biomedical Research Centre, Uferstr. 15</s1><s2>2304 Orth/Donau</s2><s3>AUT</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Autriche</country><wicri:noRegion>2304 Orth/Donau</wicri:noRegion></affiliation></author><author><name sortKey="Barrett, P Noel" sort="Barrett, P Noel" uniqKey="Barrett P" first="P. Noel" last="Barrett">P. Noel Barrett</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Baxter Vaccines, Biomedical Research Centre, Uferstr. 15</s1><s2>2304 Orth/Donau</s2><s3>AUT</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Autriche</country><wicri:noRegion>2304 Orth/Donau</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Vaccine</title><title level="j" type="abbreviated">Vaccine</title><idno type="ISSN">0264-410X</idno><imprint><date when="2006">2006</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Vaccine</title><title level="j" type="abbreviated">Vaccine</title><idno type="ISSN">0264-410X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Humoral immunity</term><term>Immune response</term><term>Severe acute respiratory syndrome virus</term><term>Vaccine</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Virus syndrome respiratoire aigu sévère</term><term>Vaccin</term><term>Immunité humorale</term><term>Réponse immune</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Vaccin</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A double-inactivated, candidate whole virus vaccine against severe acute respiratory syndrome associated coronavirus (SARS-CoV) was developed and manufactured at large scale using fermenter cultures of serum protein free Vero cells. A two step inactivation procedure involving sequential formaldehyde and U.V. inactivation was utilised in order to ensure an extremely high safety margin with respect to residual infectivity. The immunogenicity of this double-inactivated vaccine was characterised in the mouse model. Mice that were immunised twice with the candidate SARS-CoV vaccine developed high antibody titres against the SARS-CoV spike protein and high levels of neutralising antibodies. The use of the adjuvant Al(OH)<sub>3</sub> had only a minor effect on the immunogenicity of the vaccine. In addition, cell mediated immunity as measured by interferon-γ and interleukin-4 stimulation, was elicited by vaccination. Moreover, the vaccine confers protective immunity as demonstrated by prevention of SARS-CoV replication in the respiratory tract of mice after intranasal challenge with SARS-CoV. Protection of mice was correlated to antibody titre against the SARS-CoV S protein and neutralising antibody titre.</div></front></TEI><affiliations><list><country><li>Autriche</li></country></list><tree><country name="Autriche"><noRegion><name sortKey="Spruth, Martin" sort="Spruth, Martin" uniqKey="Spruth M" first="Martin" last="Spruth">Martin Spruth</name></noRegion><name sortKey="Barrett, P Noel" sort="Barrett, P Noel" uniqKey="Barrett P" first="P. Noel" last="Barrett">P. Noel Barrett</name><name sortKey="Bruhl, Peter" sort="Bruhl, Peter" uniqKey="Bruhl P" first="Peter" last="Brühl">Peter Brühl</name><name sortKey="Crowe, Brian" sort="Crowe, Brian" uniqKey="Crowe B" first="Brian" last="Crowe">Brian Crowe</name><name sortKey="Gerencer, Marijan" sort="Gerencer, Marijan" uniqKey="Gerencer M" first="Marijan" last="Gerencer">Marijan Gerencer</name><name sortKey="Grillberger, Leopold" sort="Grillberger, Leopold" uniqKey="Grillberger L" first="Leopold" last="Grillberger">Leopold Grillberger</name><name sortKey="Hitter, Elisabeth" sort="Hitter, Elisabeth" uniqKey="Hitter E" first="Elisabeth" last="Hitter">Elisabeth Hitter</name><name sortKey="Kistner, Otfried" sort="Kistner, Otfried" uniqKey="Kistner O" first="Otfried" last="Kistner">Otfried Kistner</name><name sortKey="Mundt, Wolfgang" sort="Mundt, Wolfgang" uniqKey="Mundt W" first="Wolfgang" last="Mundt">Wolfgang Mundt</name><name sortKey="Reiter, Manfred" sort="Reiter, Manfred" uniqKey="Reiter M" first="Manfred" last="Reiter">Manfred Reiter</name><name sortKey="Savidis Dacho, Helga" sort="Savidis Dacho, Helga" uniqKey="Savidis Dacho H" first="Helga" last="Savidis-Dacho">Helga Savidis-Dacho</name><name sortKey="Tauer, Christa" sort="Tauer, Christa" uniqKey="Tauer C" first="Christa" last="Tauer">Christa Tauer</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= A double-inactivated whole virus candidate SARS coronavirus vaccine stimulates neutralising and protective antibody responses
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