SrasV1, Main, Exploration, bibRecord, 004464

Design and biological activities of novel inhibitory peptides for SARS-CoV spike protein and angiotensin-converting enzyme 2 interaction

Identifieur interne : 004464 ( Main/Exploration ); précédent : 004463; suivant : 004465

Design and biological activities of novel inhibitory peptides for SARS-CoV spike protein and angiotensin-converting enzyme 2 interaction

Auteurs : Tin-Yun Ho [Taïwan] ; Shih-Lu Wu [Taïwan] ; Jaw-Chyun Chen [Taïwan] ; Yen-Chiao Wei [Taïwan] ; Shin-Ei Cheng [Taïwan] ; Yung-Hsien Chang [Taïwan] ; Hsu-Jan Liu [Taïwan] ; Chien-Yun Hsiang [Taïwan]

Source :

Antiviral research [ 0166-3542 ] ; 2006.

RBID : Pascal:06-0217624

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence, Animals, Antiviral, Biological activity, Biotinylation, Chlorocebus aethiops, Coronavirus, DNA-Binding Proteins (antagonists & inhibitors), DNA-Binding Proteins (metabolism), Enzyme-Linked Immunosorbent Assay, Humans, Interaction, Membrane Glycoproteins (antagonists & inhibitors), Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (metabolism), Molecular Sequence Data, Peptides, Peptides (chemical synthesis), Peptides (chemistry), Peptides (metabolism), Peptidyl-dipeptidase A, Protein, Recombinant Proteins (genetics), Recombinant Proteins (metabolism), SARS Virus (metabolism), SARS Virus (pathogenicity), Saccharomyces cerevisiae Proteins (antagonists & inhibitors), Saccharomyces cerevisiae Proteins (metabolism), Severe Acute Respiratory Syndrome (virology), Severe acute respiratory syndrome, Spike Glycoprotein, Coronavirus, Transcription Factors (antagonists & inhibitors), Transcription Factors (metabolism), Vero Cells, Viral Envelope Proteins (antagonists & inhibitors), Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism).
MESH :
- chemical , antagonists & inhibitors : DNA-Binding Proteins, Membrane Glycoproteins, Saccharomyces cerevisiae Proteins, Transcription Factors, Viral Envelope Proteins.
- chemical , chemical synthesis : Peptides.
- chemical , chemistry : Membrane Glycoproteins, Peptides, Viral Envelope Proteins.
- chemical , genetics : Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- chemical , metabolism : DNA-Binding Proteins, Membrane Glycoproteins, Peptides, Recombinant Proteins, Saccharomyces cerevisiae Proteins, Transcription Factors, Viral Envelope Proteins.
- metabolism : SARS Virus.
- pathogenicity : SARS Virus.
- virology : Severe Acute Respiratory Syndrome.
- Amino Acid Sequence, Animals, Biotinylation, Chlorocebus aethiops, Enzyme-Linked Immunosorbent Assay, Humans, Molecular Sequence Data, Spike Glycoprotein, Coronavirus, Vero Cells.

Abstract

Severe acute respiratory syndrome (SARS) is an emerging infectious disease caused by a novel coronavirus (SARS-CoV). The binding of SARS-CoV spike (S) protein to cellular angiotensin-converting enzyme 2 (ACE2) is the first step in SARS-CoV infection. Therefore, we assayed the inhibitory effects of small peptides derived from S protein on the binding of S protein to ACE2 and on the S-protein-pseudotyped retrovirus infectivity. SP-4 (residues 192-203), SP-8 (residues 483-494), and SP-10 (residues 668-679) significantly blocked the interaction between S protein and ACE2 by biotinylated enzyme-linked immunosorbent assay, with IC₅₀ values of 4.30 ± 2.18, 6.99 ± 0.71, and 1.88 ± 0.52 nmol, respectively. Peptide scanning suggested the region spanning residues 660-683 might act as a receptor-binding domain. SP-10 blocked both binding of the S protein and infectivity of S protein-pseudotyped retrovirus to Vero E6 cells. In conclusion, this is the first report of small peptides designed to disrupt the binding of SARS-CoV S protein to ACE2. Our findings suggest that SP-10 may be developed as an anti-SARS-CoV agent for the treatment of SARS-CoV infection.

Affiliations:

Taïwan

Le document en format XML

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<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is an emerging infectious disease caused by a novel coronavirus (SARS-CoV). The binding of SARS-CoV spike (S) protein to cellular angiotensin-converting enzyme 2 (ACE2) is the first step in SARS-CoV infection. Therefore, we assayed the inhibitory effects of small peptides derived from S protein on the binding of S protein to ACE2 and on the S-protein-pseudotyped retrovirus infectivity. SP-4 (residues 192-203), SP-8 (residues 483-494), and SP-10 (residues 668-679) significantly blocked the interaction between S protein and ACE2 by biotinylated enzyme-linked immunosorbent assay, with IC<sub>50</sub>
 values of 4.30 ± 2.18, 6.99 ± 0.71, and 1.88 ± 0.52 nmol, respectively. Peptide scanning suggested the region spanning residues 660-683 might act as a receptor-binding domain. SP-10 blocked both binding of the S protein and infectivity of S protein-pseudotyped retrovirus to Vero E6 cells. In conclusion, this is the first report of small peptides designed to disrupt the binding of SARS-CoV S protein to ACE2. Our findings suggest that SP-10 may be developed as an anti-SARS-CoV agent for the treatment of SARS-CoV infection.</div>
</front>
</TEI>
<affiliations><list><country><li>Taïwan</li>
</country>
</list>
<tree><country name="Taïwan"><noRegion><name sortKey="Ho, Tin Yun" sort="Ho, Tin Yun" uniqKey="Ho T" first="Tin-Yun" last="Ho">Tin-Yun Ho</name>
</noRegion>
<name sortKey="Chang, Yung Hsien" sort="Chang, Yung Hsien" uniqKey="Chang Y" first="Yung-Hsien" last="Chang">Yung-Hsien Chang</name>
<name sortKey="Chen, Jaw Chyun" sort="Chen, Jaw Chyun" uniqKey="Chen J" first="Jaw-Chyun" last="Chen">Jaw-Chyun Chen</name>
<name sortKey="Cheng, Shin Ei" sort="Cheng, Shin Ei" uniqKey="Cheng S" first="Shin-Ei" last="Cheng">Shin-Ei Cheng</name>
<name sortKey="Hsiang, Chien Yun" sort="Hsiang, Chien Yun" uniqKey="Hsiang C" first="Chien-Yun" last="Hsiang">Chien-Yun Hsiang</name>
<name sortKey="Liu, Hsu Jan" sort="Liu, Hsu Jan" uniqKey="Liu H" first="Hsu-Jan" last="Liu">Hsu-Jan Liu</name>
<name sortKey="Wei, Yen Chiao" sort="Wei, Yen Chiao" uniqKey="Wei Y" first="Yen-Chiao" last="Wei">Yen-Chiao Wei</name>
<name sortKey="Wu, Shih Lu" sort="Wu, Shih Lu" uniqKey="Wu S" first="Shih-Lu" last="Wu">Shih-Lu Wu</name>
</country>
</tree>
</affiliations>
</record>

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Design and biological activities of novel inhibitory peptides for SARS-CoV spike protein and angiotensin-converting enzyme 2 interaction

Design and biological activities of novel inhibitory peptides for SARS-CoV spike protein and angiotensin-converting enzyme 2 interaction

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