SrasV1, Main, Exploration, bibRecord, 004445

Human memory T cell responses to SARS-CoV E protein

Identifieur interne : 004445 ( Main/Exploration ); précédent : 004444; suivant : 004446

Human memory T cell responses to SARS-CoV E protein

Auteurs : HUI PENG [République populaire de Chine] ; Li-Tao Yang [République populaire de Chine] ; JIAN LI [République populaire de Chine] ; Zhi-Qiang Lu [République populaire de Chine] ; Ling-Yun Wang [République populaire de Chine] ; Richard A. Koup [États-Unis] ; Robert T. Bailer [États-Unis] ; Chang-You Wu [République populaire de Chine]

Source :

Microbes and infection [ 1286-4579 ] ; 2006.

RBID : Pascal:06-0518406

Descripteurs français

Pascal (Inist)
- Homme, Virus syndrome respiratoire aigu sévère, Immunité cellulaire, Protéine, Lymphocyte T, Déterminant antigénique, Syndrome respiratoire aigu sévère.
Wicri :
- topic : Homme.

English descriptors

KwdEn :
- Antigenic determinant, Cellular immunity, Human, Protein, Severe acute respiratory syndrome, Severe acute respiratory syndrome virus, T-Lymphocyte.

Abstract

E protein is a membrane component of severe acute respiratory syndrome coronavirus (SARS-CoV). Disruption of E protein may reduce viral infectivity. Thus, the SARS-CoV E protein is considered a potential target for the development of antiviral drugs. However, the cellular immune responses to E protein remain unclear in humans. In this study, we found that peripheral blood mononuclear cells (PBMCs) from fully recovered SARS individuals rapidly produced IFN-y and IL-2 following stimulation with a pool of 9 peptides overlapping the entire E protein sequence. Analysis of the immune responses by flow cytometry showed that both CD4⁺ and CD8⁺T cells were involved in the SARS-CoV E-specific immune responses after stimulation with SARS-CoV E peptides. Moreover, the majority of IFN-γ⁺CD4+T cells were central memory cells expressing CD45RO⁺CCR7+CD62L^-; whereas IFN-γ⁺CD8⁺ memory T cells were mostly effector memory cells expressing CD45RO^-CCR7^-CD62L^-. The results of T-cell responses to 9 individual peptides indicated that the E protein contained at least two major T cell epitopes (E2 amino acid [aa] 9-26 and E5-6: aa 33-57) which were important in eliciting cellular immune response to SARS-CoV E protein in humans.

Affiliations:

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Le document en format XML

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<author><name sortKey="Wu, Chang You" sort="Wu, Chang You" uniqKey="Wu C" first="Chang-You" last="Wu">Chang-You Wu</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, No. 74 Zhongshan Road II</s1>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Human memory T cell responses to SARS-CoV E protein</title>
<author><name sortKey="Hui Peng" sort="Hui Peng" uniqKey="Hui Peng" last="Hui Peng">HUI PENG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, No. 74 Zhongshan Road II</s1>
<s2>Guangzhou 510089</s2>
<s3>CHN</s3>
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<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
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<country>République populaire de Chine</country>
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<author><name sortKey="Yang, Li Tao" sort="Yang, Li Tao" uniqKey="Yang L" first="Li-Tao" last="Yang">Li-Tao Yang</name>
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<s2>Guangzhou 510089</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
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<author><name sortKey="Jian Li" sort="Jian Li" uniqKey="Jian Li" last="Jian Li">JIAN LI</name>
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<author><name sortKey="Lu, Zhi Qiang" sort="Lu, Zhi Qiang" uniqKey="Lu Z" first="Zhi-Qiang" last="Lu">Zhi-Qiang Lu</name>
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<s2>Guangzhou 510020</s2>
<s3>CHN</s3>
<sZ>4 aut.</sZ>
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<country>République populaire de Chine</country>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
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</affiliation>
</author>
<author><name sortKey="Wang, Ling Yun" sort="Wang, Ling Yun" uniqKey="Wang L" first="Ling-Yun" last="Wang">Ling-Yun Wang</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Digestive Diseases, the Second Affiliated Hospital of Sun Yat-sen University, No. 107 Yanjiang Road</s1>
<s2>Guangzhou 510020</s2>
<s3>CHN</s3>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Koup, Richard A" sort="Koup, Richard A" uniqKey="Koup R" first="Richard A." last="Koup">Richard A. Koup</name>
<affiliation wicri:level="2"><inist:fA14 i1="05"><s1>Vaccine Research Center, NIAID/NIH</s1>
<s2>Bethesda, MD 20892</s2>
<s3>USA</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
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<placeName><region type="state">Maryland</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bailer, Robert T" sort="Bailer, Robert T" uniqKey="Bailer R" first="Robert T." last="Bailer">Robert T. Bailer</name>
<affiliation wicri:level="2"><inist:fA14 i1="05"><s1>Vaccine Research Center, NIAID/NIH</s1>
<s2>Bethesda, MD 20892</s2>
<s3>USA</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<placeName><region type="state">Maryland</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wu, Chang You" sort="Wu, Chang You" uniqKey="Wu C" first="Chang-You" last="Wu">Chang-You Wu</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, No. 74 Zhongshan Road II</s1>
<s2>Guangzhou 510089</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
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<series><title level="j" type="main">Microbes and infection</title>
<title level="j" type="abbreviated">Microbes infect.</title>
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<imprint><date when="2006">2006</date>
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<seriesStmt><title level="j" type="main">Microbes and infection</title>
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<idno type="ISSN">1286-4579</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antigenic determinant</term>
<term>Cellular immunity</term>
<term>Human</term>
<term>Protein</term>
<term>Severe acute respiratory syndrome</term>
<term>Severe acute respiratory syndrome virus</term>
<term>T-Lymphocyte</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Homme</term>
<term>Virus syndrome respiratoire aigu sévère</term>
<term>Immunité cellulaire</term>
<term>Protéine</term>
<term>Lymphocyte T</term>
<term>Déterminant antigénique</term>
<term>Syndrome respiratoire aigu sévère</term>
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<front><div type="abstract" xml:lang="en">E protein is a membrane component of severe acute respiratory syndrome coronavirus (SARS-CoV). Disruption of E protein may reduce viral infectivity. Thus, the SARS-CoV E protein is considered a potential target for the development of antiviral drugs. However, the cellular immune responses to E protein remain unclear in humans. In this study, we found that peripheral blood mononuclear cells (PBMCs) from fully recovered SARS individuals rapidly produced IFN-y and IL-2 following stimulation with a pool of 9 peptides overlapping the entire E protein sequence. Analysis of the immune responses by flow cytometry showed that both CD4<sup>+</sup>
 and CD8<sup>+</sup>
T cells were involved in the SARS-CoV E-specific immune responses after stimulation with SARS-CoV E peptides. Moreover, the majority of IFN-γ<sup>+</sup>
CD4+T cells were central memory cells expressing CD45RO<sup>+</sup>
CCR7+CD62L<sup>-</sup>
; whereas IFN-γ<sup>+</sup>
CD8<sup>+</sup>
 memory T cells were mostly effector memory cells expressing CD45RO<sup>-</sup>
CCR7<sup>-</sup>
CD62L<sup>-</sup>
. The results of T-cell responses to 9 individual peptides indicated that the E protein contained at least two major T cell epitopes (E2 amino acid [aa] 9-26 and E5-6: aa 33-57) which were important in eliciting cellular immune response to SARS-CoV E protein in humans.</div>
</front>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
<li>États-Unis</li>
</country>
<region><li>Guangdong</li>
<li>Maryland</li>
</region>
<settlement><li>Jiangmen</li>
</settlement>
</list>
<tree><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Hui Peng" sort="Hui Peng" uniqKey="Hui Peng" last="Hui Peng">HUI PENG</name>
</region>
<name sortKey="Jian Li" sort="Jian Li" uniqKey="Jian Li" last="Jian Li">JIAN LI</name>
<name sortKey="Lu, Zhi Qiang" sort="Lu, Zhi Qiang" uniqKey="Lu Z" first="Zhi-Qiang" last="Lu">Zhi-Qiang Lu</name>
<name sortKey="Wang, Ling Yun" sort="Wang, Ling Yun" uniqKey="Wang L" first="Ling-Yun" last="Wang">Ling-Yun Wang</name>
<name sortKey="Wu, Chang You" sort="Wu, Chang You" uniqKey="Wu C" first="Chang-You" last="Wu">Chang-You Wu</name>
<name sortKey="Yang, Li Tao" sort="Yang, Li Tao" uniqKey="Yang L" first="Li-Tao" last="Yang">Li-Tao Yang</name>
</country>
<country name="États-Unis"><region name="Maryland"><name sortKey="Koup, Richard A" sort="Koup, Richard A" uniqKey="Koup R" first="Richard A." last="Koup">Richard A. Koup</name>
</region>
<name sortKey="Bailer, Robert T" sort="Bailer, Robert T" uniqKey="Bailer R" first="Robert T." last="Bailer">Robert T. Bailer</name>
</country>
</tree>
</affiliations>
</record>

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Human memory T cell responses to SARS-CoV E protein

Human memory T cell responses to SARS-CoV E protein

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri