SrasV1, Main, Exploration, bibRecord, 003F46

Comparative study of the effects of heptameric slippery site composition on -1 frameshifting among different eukaryotic systems.

Identifieur interne : 003F46 ( Main/Exploration ); précédent : 003F45; suivant : 003F47

Comparative study of the effects of heptameric slippery site composition on -1 frameshifting among different eukaryotic systems.

Auteurs : Ewan P. Plant [États-Unis] ; Jonathan D. Dinman

Source :

RNA (New York, N.Y.) [ 1355-8382 ] ; 2006.

RBID : pubmed:16497657

Descripteurs français

KwdFr :
- Codon, Décalage ribosomique du cadre de lecture, Escherichia coli (génétique), Humains, Lignée cellulaire, Lymphocytes T (métabolisme), Mutagenèse dirigée, Oligonucléotides, Plasmides, Séquence nucléotidique, VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
MESH :
- génétique : Escherichia coli.
- métabolisme : Lymphocytes T.
- Codon, Décalage ribosomique du cadre de lecture, Humains, Lignée cellulaire, Mutagenèse dirigée, Oligonucléotides, Plasmides, Séquence nucléotidique, VIH-1 (Virus de l'Immunodéficience Humaine de type 1).

English descriptors

KwdEn :
- Base Sequence, Cell Line, Codon, Escherichia coli (genetics), Frameshifting, Ribosomal, HIV-1, Humans, Mutagenesis, Site-Directed, Oligonucleotides, Plasmids, T-Lymphocytes (metabolism).
MESH :
- chemical : Codon, Oligonucleotides.
- genetics : Escherichia coli.
- metabolism : T-Lymphocytes.
- Base Sequence, Cell Line, Frameshifting, Ribosomal, HIV-1, Humans, Mutagenesis, Site-Directed, Plasmids.

Abstract

Studies of programmed -1 ribosomal frameshifting (-1 PRF) have been approached over the past two decades by many different laboratories using a diverse array of virus-derived frameshift signals in translational assay systems derived from a variety of sources. Though it is generally acknowledged that both absolute and relative -1 PRF efficiency can vary in an assay system-dependent manner, no methodical study of this phenomenon has been undertaken. To address this issue, a series of slippery site mutants of the SARS-associated coronavirus frameshift signal were systematically assayed in four different eukaryotic translational systems. HIV-1 promoted frameshifting was also compared between Escherichia coli and a human T-cell line expression systems. The results of these analyses highlight different aspects of each system, suggesting in general that (1) differences can be due to the assay systems themselves; (2) phylogenetic differences in ribosome structure can affect frameshifting efficiency; and (3) care must be taken to employ the closest phylogenetic match between a specific -1 PRF signal and the choice of translational assay system.

DOI: 10.1261/rna.2225206
PubMed: 16497657

Affiliations:

Le document en format XML

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<term>Mutagenèse dirigée</term>
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<front><div type="abstract" xml:lang="en">Studies of programmed -1 ribosomal frameshifting (-1 PRF) have been approached over the past two decades by many different laboratories using a diverse array of virus-derived frameshift signals in translational assay systems derived from a variety of sources. Though it is generally acknowledged that both absolute and relative -1 PRF efficiency can vary in an assay system-dependent manner, no methodical study of this phenomenon has been undertaken. To address this issue, a series of slippery site mutants of the SARS-associated coronavirus frameshift signal were systematically assayed in four different eukaryotic translational systems. HIV-1 promoted frameshifting was also compared between Escherichia coli and a human T-cell line expression systems. The results of these analyses highlight different aspects of each system, suggesting in general that (1) differences can be due to the assay systems themselves; (2) phylogenetic differences in ribosome structure can affect frameshifting efficiency; and (3) care must be taken to employ the closest phylogenetic match between a specific -1 PRF signal and the choice of translational assay system.</div>
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Comparative study of the effects of heptameric slippery site composition on -1 frameshifting among different eukaryotic systems.

Comparative study of the effects of heptameric slippery site composition on -1 frameshifting among different eukaryotic systems.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration SRAS
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