Comparative study of the effects of heptameric slippery site composition on -1 frameshifting among different eukaryotic systems.
Identifieur interne : 001393 ( Ncbi/Merge ); précédent : 001392; suivant : 001394Comparative study of the effects of heptameric slippery site composition on -1 frameshifting among different eukaryotic systems.
Auteurs : Ewan P. Plant [États-Unis] ; Jonathan D. DinmanSource :
- RNA (New York, N.Y.) [ 1355-8382 ] ; 2006.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical : Codon, Oligonucleotides.
- genetics : Escherichia coli.
- metabolism : T-Lymphocytes.
- Base Sequence, Cell Line, Frameshifting, Ribosomal, HIV-1, Humans, Mutagenesis, Site-Directed, Plasmids.
Abstract
Studies of programmed -1 ribosomal frameshifting (-1 PRF) have been approached over the past two decades by many different laboratories using a diverse array of virus-derived frameshift signals in translational assay systems derived from a variety of sources. Though it is generally acknowledged that both absolute and relative -1 PRF efficiency can vary in an assay system-dependent manner, no methodical study of this phenomenon has been undertaken. To address this issue, a series of slippery site mutants of the SARS-associated coronavirus frameshift signal were systematically assayed in four different eukaryotic translational systems. HIV-1 promoted frameshifting was also compared between Escherichia coli and a human T-cell line expression systems. The results of these analyses highlight different aspects of each system, suggesting in general that (1) differences can be due to the assay systems themselves; (2) phylogenetic differences in ribosome structure can affect frameshifting efficiency; and (3) care must be taken to employ the closest phylogenetic match between a specific -1 PRF signal and the choice of translational assay system.
DOI: 10.1261/rna.2225206
PubMed: 16497657
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Plant</name><affiliation wicri:level="4"><nlm:affiliation>Department of Cell Biology and Molecular Genetics, Microbiology Building, Room 2135, University of Maryland, College Park, Maryland 20742, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Cell Biology and Molecular Genetics, Microbiology Building, Room 2135, University of Maryland, College Park, Maryland 20742</wicri:regionArea><orgName type="university">Université du Maryland</orgName><placeName><settlement type="city">College Park (Maryland)</settlement><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Dinman, Jonathan D" sort="Dinman, Jonathan D" uniqKey="Dinman J" first="Jonathan D" last="Dinman">Jonathan D. 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Plant</name><affiliation wicri:level="4"><nlm:affiliation>Department of Cell Biology and Molecular Genetics, Microbiology Building, Room 2135, University of Maryland, College Park, Maryland 20742, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Cell Biology and Molecular Genetics, Microbiology Building, Room 2135, University of Maryland, College Park, Maryland 20742</wicri:regionArea><orgName type="university">Université du Maryland</orgName><placeName><settlement type="city">College Park (Maryland)</settlement><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Dinman, Jonathan D" sort="Dinman, Jonathan D" uniqKey="Dinman J" first="Jonathan D" last="Dinman">Jonathan D. Dinman</name></author></analytic><series><title level="j">RNA (New York, N.Y.)</title><idno type="ISSN">1355-8382</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>Cell Line</term><term>Codon</term><term>Escherichia coli (genetics)</term><term>Frameshifting, Ribosomal</term><term>HIV-1</term><term>Humans</term><term>Mutagenesis, Site-Directed</term><term>Oligonucleotides</term><term>Plasmids</term><term>T-Lymphocytes (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Codon</term><term>Décalage ribosomique du cadre de lecture</term><term>Escherichia coli (génétique)</term><term>Humains</term><term>Lignée cellulaire</term><term>Lymphocytes T (métabolisme)</term><term>Mutagenèse dirigée</term><term>Oligonucléotides</term><term>Plasmides</term><term>Séquence nucléotidique</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Codon</term><term>Oligonucleotides</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Lymphocytes T</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Cell Line</term><term>Frameshifting, Ribosomal</term><term>HIV-1</term><term>Humans</term><term>Mutagenesis, Site-Directed</term><term>Plasmids</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Codon</term><term>Décalage ribosomique du cadre de lecture</term><term>Humains</term><term>Lignée cellulaire</term><term>Mutagenèse dirigée</term><term>Oligonucléotides</term><term>Plasmides</term><term>Séquence nucléotidique</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Studies of programmed -1 ribosomal frameshifting (-1 PRF) have been approached over the past two decades by many different laboratories using a diverse array of virus-derived frameshift signals in translational assay systems derived from a variety of sources. Though it is generally acknowledged that both absolute and relative -1 PRF efficiency can vary in an assay system-dependent manner, no methodical study of this phenomenon has been undertaken. To address this issue, a series of slippery site mutants of the SARS-associated coronavirus frameshift signal were systematically assayed in four different eukaryotic translational systems. HIV-1 promoted frameshifting was also compared between Escherichia coli and a human T-cell line expression systems. The results of these analyses highlight different aspects of each system, suggesting in general that (1) differences can be due to the assay systems themselves; (2) phylogenetic differences in ribosome structure can affect frameshifting efficiency; and (3) care must be taken to employ the closest phylogenetic match between a specific -1 PRF signal and the choice of translational assay system.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16497657</PMID><DateCompleted><Year>2006</Year><Month>06</Month><Day>06</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1355-8382</ISSN><JournalIssue CitedMedium="Print"><Volume>12</Volume><Issue>4</Issue><PubDate><Year>2006</Year><Month>Apr</Month></PubDate></JournalIssue><Title>RNA (New York, N.Y.)</Title><ISOAbbreviation>RNA</ISOAbbreviation></Journal><ArticleTitle>Comparative study of the effects of heptameric slippery site composition on -1 frameshifting among different eukaryotic systems.</ArticleTitle><Pagination><MedlinePgn>666-73</MedlinePgn></Pagination><Abstract><AbstractText>Studies of programmed -1 ribosomal frameshifting (-1 PRF) have been approached over the past two decades by many different laboratories using a diverse array of virus-derived frameshift signals in translational assay systems derived from a variety of sources. Though it is generally acknowledged that both absolute and relative -1 PRF efficiency can vary in an assay system-dependent manner, no methodical study of this phenomenon has been undertaken. To address this issue, a series of slippery site mutants of the SARS-associated coronavirus frameshift signal were systematically assayed in four different eukaryotic translational systems. HIV-1 promoted frameshifting was also compared between Escherichia coli and a human T-cell line expression systems. The results of these analyses highlight different aspects of each system, suggesting in general that (1) differences can be due to the assay systems themselves; (2) phylogenetic differences in ribosome structure can affect frameshifting efficiency; and (3) care must be taken to employ the closest phylogenetic match between a specific -1 PRF signal and the choice of translational assay system.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Plant</LastName><ForeName>Ewan P</ForeName><Initials>EP</Initials><AffiliationInfo><Affiliation>Department of Cell Biology and Molecular Genetics, Microbiology Building, Room 2135, University of Maryland, College Park, Maryland 20742, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dinman</LastName><ForeName>Jonathan D</ForeName><Initials>JD</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>R01 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Dinman</name></noCountry><country name="États-Unis"><region name="Maryland"><name sortKey="Plant, Ewan P" sort="Plant, Ewan P" uniqKey="Plant E" first="Ewan P" last="Plant">Ewan P. Plant</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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