Production of an anti-severe acute respiratory syndrome (SARS) coronavirus human monoclonal antibody Fab fragment by using a combinatorial immunoglobulin gene library derived from patients who recovered from SARS.
Identifieur interne : 003D70 ( Main/Exploration ); précédent : 003D69; suivant : 003D71Production of an anti-severe acute respiratory syndrome (SARS) coronavirus human monoclonal antibody Fab fragment by using a combinatorial immunoglobulin gene library derived from patients who recovered from SARS.
Auteurs : Jinye Liu [République populaire de Chine] ; Hongxia Shao ; Yanlin Tao ; Bin Yang ; Lisheng Qian ; Xiaoli Yang ; Brian Cao ; Gengxi Hu ; Hiroshi Tachibana ; Xunjia ChengSource :
- Clinical and vaccine immunology : CVI [ 1556-6811 ] ; 2006.
Descripteurs français
- KwdFr :
- Anticorps antiviraux (génétique), Anticorps antiviraux (immunologie), Anticorps monoclonaux (), Anticorps monoclonaux (génétique), Anticorps monoclonaux (métabolisme), Banque génomique, Coronavirus (immunologie), Données de séquences moléculaires, Fragments Fab d'immunoglobuline (), Fragments Fab d'immunoglobuline (génétique), Fragments Fab d'immunoglobuline (métabolisme), Humains, Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (virologie), Séquence d'acides aminés, Techniques de chimie combinatoire.
- MESH :
- génétique : Anticorps antiviraux, Anticorps monoclonaux, Fragments Fab d'immunoglobuline.
- immunologie : Anticorps antiviraux, Coronavirus, Syndrome respiratoire aigu sévère.
- métabolisme : Anticorps monoclonaux, Fragments Fab d'immunoglobuline.
- virologie : Syndrome respiratoire aigu sévère.
- Anticorps monoclonaux, Banque génomique, Données de séquences moléculaires, Fragments Fab d'immunoglobuline, Humains, Séquence d'acides aminés, Techniques de chimie combinatoire.
English descriptors
- KwdEn :
- Amino Acid Sequence, Antibodies, Monoclonal (chemistry), Antibodies, Monoclonal (genetics), Antibodies, Monoclonal (metabolism), Antibodies, Viral (genetics), Antibodies, Viral (immunology), Combinatorial Chemistry Techniques, Coronavirus (immunology), Genomic Library, Humans, Immunoglobulin Fab Fragments (chemistry), Immunoglobulin Fab Fragments (genetics), Immunoglobulin Fab Fragments (metabolism), Molecular Sequence Data, Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology).
- MESH :
- chemical , chemistry : Antibodies, Monoclonal, Immunoglobulin Fab Fragments.
- chemical , genetics : Antibodies, Monoclonal, Antibodies, Viral, Immunoglobulin Fab Fragments.
- chemical , immunology : Antibodies, Viral.
- chemical , metabolism : Antibodies, Monoclonal, Immunoglobulin Fab Fragments.
- immunology : Coronavirus, Severe Acute Respiratory Syndrome.
- virology : Severe Acute Respiratory Syndrome.
- Amino Acid Sequence, Combinatorial Chemistry Techniques, Genomic Library, Humans, Molecular Sequence Data.
Abstract
A combinatorial human immunoglobulin gene library was constructed from the peripheral lymphocytes of two patients who recovered from severe acute respiratory syndrome (SARS). The library was screened for the production of Fab antibody fragments to a recombinant spike protein of SARS-associated coronavirus (SARS-CoV). One Fab clone, AS3-3, reacted with the spike protein in an enzyme-linked immunosorbent assay. The dissociation constant of AS3-3 was 1.98 x 10(-8) M. Immunofluorescent microscopy revealed that it reacted with SARS-CoV-infected cells. The library seems to be a potent tool for the production of human antibodies to SARS-CoV.
DOI: 10.1128/CVI.13.5.594-597.2006
PubMed: 16682481
Affiliations:
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Le document en format XML
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sort="Tao, Yanlin" uniqKey="Tao Y" first="Yanlin" last="Tao">Yanlin Tao</name></author><author><name sortKey="Yang, Bin" sort="Yang, Bin" uniqKey="Yang B" first="Bin" last="Yang">Bin Yang</name></author><author><name sortKey="Qian, Lisheng" sort="Qian, Lisheng" uniqKey="Qian L" first="Lisheng" last="Qian">Lisheng Qian</name></author><author><name sortKey="Yang, Xiaoli" sort="Yang, Xiaoli" uniqKey="Yang X" first="Xiaoli" last="Yang">Xiaoli Yang</name></author><author><name sortKey="Cao, Brian" sort="Cao, Brian" uniqKey="Cao B" first="Brian" last="Cao">Brian Cao</name></author><author><name sortKey="Hu, Gengxi" sort="Hu, Gengxi" uniqKey="Hu G" first="Gengxi" last="Hu">Gengxi Hu</name></author><author><name sortKey="Tachibana, Hiroshi" sort="Tachibana, Hiroshi" uniqKey="Tachibana H" first="Hiroshi" last="Tachibana">Hiroshi Tachibana</name></author><author><name sortKey="Cheng, Xunjia" sort="Cheng, Xunjia" uniqKey="Cheng X" first="Xunjia" last="Cheng">Xunjia 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type="abstract" xml:lang="en">A combinatorial human immunoglobulin gene library was constructed from the peripheral lymphocytes of two patients who recovered from severe acute respiratory syndrome (SARS). The library was screened for the production of Fab antibody fragments to a recombinant spike protein of SARS-associated coronavirus (SARS-CoV). One Fab clone, AS3-3, reacted with the spike protein in an enzyme-linked immunosorbent assay. The dissociation constant of AS3-3 was 1.98 x 10(-8) M. Immunofluorescent microscopy revealed that it reacted with SARS-CoV-infected cells. The library seems to be a potent tool for the production of human antibodies to SARS-CoV.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Cao, Brian" sort="Cao, Brian" uniqKey="Cao B" first="Brian" last="Cao">Brian Cao</name><name sortKey="Cheng, Xunjia" sort="Cheng, Xunjia" uniqKey="Cheng X" first="Xunjia" last="Cheng">Xunjia Cheng</name><name sortKey="Hu, Gengxi" sort="Hu, Gengxi" uniqKey="Hu G" first="Gengxi" last="Hu">Gengxi Hu</name><name sortKey="Qian, Lisheng" sort="Qian, Lisheng" uniqKey="Qian L" first="Lisheng" last="Qian">Lisheng Qian</name><name sortKey="Shao, Hongxia" sort="Shao, Hongxia" uniqKey="Shao H" first="Hongxia" last="Shao">Hongxia Shao</name><name sortKey="Tachibana, Hiroshi" sort="Tachibana, Hiroshi" uniqKey="Tachibana H" first="Hiroshi" last="Tachibana">Hiroshi Tachibana</name><name sortKey="Tao, Yanlin" sort="Tao, Yanlin" uniqKey="Tao Y" first="Yanlin" last="Tao">Yanlin Tao</name><name sortKey="Yang, Bin" sort="Yang, Bin" uniqKey="Yang B" first="Bin" last="Yang">Bin Yang</name><name sortKey="Yang, Xiaoli" sort="Yang, Xiaoli" uniqKey="Yang X" first="Xiaoli" last="Yang">Xiaoli Yang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Liu, Jinye" sort="Liu, Jinye" uniqKey="Liu J" first="Jinye" last="Liu">Jinye Liu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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