A line immunoassay utilizing recombinant nucleocapsid proteins for detection of antibodies to human coronaviruses
Identifieur interne : 003522 ( Main/Exploration ); précédent : 003521; suivant : 003523A line immunoassay utilizing recombinant nucleocapsid proteins for detection of antibodies to human coronaviruses
Auteurs : Christian Lehmann [Allemagne] ; Hans Wolf [Allemagne] ; JIANGUO XU [République populaire de Chine] ; QUANBI ZHAO [République populaire de Chine] ; YIMING SHAO [République populaire de Chine] ; Manfred Motz [Allemagne] ; Petra Lindner [Allemagne]Source :
- Diagnostic microbiology and infectious disease [ 0732-8893 ] ; 2008.
Descripteurs français
- KwdFr :
- Anticorps antiviraux (sang), Coronavirus (immunologie), Dosage immunologique (), Humains, Immunoglobuline G (sang), Infections à coronavirus (diagnostic), Infections à coronavirus (immunologie), Protéines nucléocapside (immunologie), Protéines recombinantes (immunologie), Réactions croisées, Sensibilité et spécificité.
- MESH :
- diagnostic : Infections à coronavirus.
- immunologie : Coronavirus, Infections à coronavirus, Protéines nucléocapside, Protéines recombinantes.
- sang : Anticorps antiviraux, Immunoglobuline G.
- Pascal (Inist)
- Wicri :
- topic : Homme.
English descriptors
- KwdEn :
- Antibodies, Viral (blood), Antibody, Coronavirus, Coronavirus (immunology), Coronavirus Infections (diagnosis), Coronavirus Infections (immunology), Cross Reactions, Cross reaction, Detection, Human, Humans, Immunoassay (methods), Immunoglobulin G (blood), Infection, Microbiology, Nucleocapsid, Nucleocapsid Proteins (immunology), Prevalence, Recombinant Proteins (immunology), Recombinant protein, Sensitivity and Specificity, Serology.
- MESH :
- chemical , blood : Antibodies, Viral, Immunoglobulin G.
- diagnosis : Coronavirus Infections.
- immunology : Coronavirus, Coronavirus Infections, Nucleocapsid Proteins, Recombinant Proteins.
- methods : Immunoassay.
- Cross Reactions, Humans, Sensitivity and Specificity.
Abstract
Most coronaviruses infecting humans cause mild diseases, whereas severe acute respiratory syndrome (SARS)-associated coronavirus is an extremely dangerous pathogen. Here, we report the development of a serologic assay for detection of antibodies to human coronaviruses (HCoVs) based on recombinant nucleocapsid (N) proteins of all known pathogenic strains (229E, NL63, OC43, HKU1, SARS). The novel immunoassay is highly useful for epidemiologic surveys, where use of nucleic acid diagnostics often is limited. Purified recombinant antigens were immobilized on nitrocellulose membranes and applied in a line immunoassay, which allows rapid detection of antibodies to 5 different HCoVs in a single experiment. For assay evaluation, serum samples from persons infected with 229E or OC43 (acute/convalescent), recovered SARS patients and healthy donors were analyzed. Screening for nucleocapsid (N)-specific immunoglobulin G (IgG) in convalescent sera reached 100% sensitivity. With this new technique, we found that recently identified NL63 and HKU1 contribute significantly to the overall spectrum of coronavirus infections. Possibly, cross-reactive antibody responses were observed using 229E and OC43 serum pairs. However, the potential of this assay could clearly be demonstrated employing SARS-positive serum samples, where nonspecific binding to nucleocapsids of other HCoVs was not observed. This coronavirus strain-specific line immunoassay represents a powerful tool for serologic diagnostics.
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Affiliations:
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coronaviruses</title><author><name sortKey="Lehmann, Christian" sort="Lehmann, Christian" uniqKey="Lehmann C" first="Christian" last="Lehmann">Christian Lehmann</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute for Medical Microbiology and Hygiene, University of Regensburg</s1><s2>93053 Regensburg</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>93053 Regensburg</wicri:noRegion><wicri:noRegion>University of Regensburg</wicri:noRegion><wicri:noRegion>93053 Regensburg</wicri:noRegion></affiliation></author><author><name sortKey="Wolf, Hans" sort="Wolf, Hans" uniqKey="Wolf H" first="Hans" last="Wolf">Hans Wolf</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute for Medical Microbiology and Hygiene, University of Regensburg</s1><s2>93053 Regensburg</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>93053 Regensburg</wicri:noRegion><wicri:noRegion>University of Regensburg</wicri:noRegion><wicri:noRegion>93053 Regensburg</wicri:noRegion></affiliation></author><author><name sortKey="Jianguo Xu" sort="Jianguo Xu" uniqKey="Jianguo Xu" last="Jianguo Xu">JIANGUO XU</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Chinese Center for Disease Control and Prevention (China CDC)</s1><s2>Beijing</s2><s3>CHN</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Quanbi Zhao" sort="Quanbi Zhao" uniqKey="Quanbi Zhao" last="Quanbi Zhao">QUANBI ZHAO</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Chinese Center for Disease Control and Prevention (China CDC)</s1><s2>Beijing</s2><s3>CHN</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Yiming Shao" sort="Yiming Shao" uniqKey="Yiming Shao" last="Yiming Shao">YIMING SHAO</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Chinese Center for Disease Control and Prevention (China CDC)</s1><s2>Beijing</s2><s3>CHN</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Motz, Manfred" sort="Motz, Manfred" uniqKey="Motz M" first="Manfred" last="Motz">Manfred Motz</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Mikrogen GmbH</s1><s2>82061 Neuried</s2><s3>DEU</s3><sZ>6 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>82061 Neuried</wicri:noRegion><wicri:noRegion>Mikrogen GmbH</wicri:noRegion><wicri:noRegion>Mikrogen GmbH</wicri:noRegion></affiliation></author><author><name sortKey="Lindner, Petra" sort="Lindner, Petra" uniqKey="Lindner P" first="Petra" last="Lindner">Petra Lindner</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute for Medical Microbiology and Hygiene, University of Regensburg</s1><s2>93053 Regensburg</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>93053 Regensburg</wicri:noRegion><wicri:noRegion>University of Regensburg</wicri:noRegion><wicri:noRegion>93053 Regensburg</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Diagnostic microbiology and infectious disease</title><title level="j" type="abbreviated">Diagn. microbiol. infect. dis.</title><idno type="ISSN">0732-8893</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Diagnostic microbiology and infectious disease</title><title level="j" type="abbreviated">Diagn. microbiol. infect. dis.</title><idno 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()</term><term>Humains</term><term>Immunoglobuline G (sang)</term><term>Infections à coronavirus (diagnostic)</term><term>Infections à coronavirus (immunologie)</term><term>Protéines nucléocapside (immunologie)</term><term>Protéines recombinantes (immunologie)</term><term>Réactions croisées</term><term>Sensibilité et spécificité</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Coronavirus</term><term>Infections à coronavirus</term><term>Protéines nucléocapside</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Coronavirus</term><term>Coronavirus Infections</term><term>Nucleocapsid Proteins</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Immunoassay</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term><term>Immunoglobuline G</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cross Reactions</term><term>Humans</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Dosage immunologique</term><term>Homme</term><term>Coronavirus</term><term>Humains</term><term>Protéine recombinante</term><term>Nucléocapside</term><term>Détection</term><term>Anticorps</term><term>Prévalence</term><term>Réactions croisées</term><term>Sensibilité et spécificité</term><term>Sérologie</term><term>Réaction croisée</term><term>Microbiologie</term><term>Infection</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Most coronaviruses infecting humans cause mild diseases, whereas severe acute respiratory syndrome (SARS)-associated coronavirus is an extremely dangerous pathogen. Here, we report the development of a serologic assay for detection of antibodies to human coronaviruses (HCoVs) based on recombinant nucleocapsid (N) proteins of all known pathogenic strains (229E, NL63, OC43, HKU1, SARS). The novel immunoassay is highly useful for epidemiologic surveys, where use of nucleic acid diagnostics often is limited. Purified recombinant antigens were immobilized on nitrocellulose membranes and applied in a line immunoassay, which allows rapid detection of antibodies to 5 different HCoVs in a single experiment. For assay evaluation, serum samples from persons infected with 229E or OC43 (acute/convalescent), recovered SARS patients and healthy donors were analyzed. Screening for nucleocapsid (N)-specific immunoglobulin G (IgG) in convalescent sera reached 100% sensitivity. With this new technique, we found that recently identified NL63 and HKU1 contribute significantly to the overall spectrum of coronavirus infections. Possibly, cross-reactive antibody responses were observed using 229E and OC43 serum pairs. However, the potential of this assay could clearly be demonstrated employing SARS-positive serum samples, where nonspecific binding to nucleocapsids of other HCoVs was not observed. This coronavirus strain-specific line immunoassay represents a powerful tool for serologic diagnostics.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="Allemagne"><noRegion><name sortKey="Lehmann, Christian" sort="Lehmann, Christian" uniqKey="Lehmann C" first="Christian" last="Lehmann">Christian Lehmann</name></noRegion><name sortKey="Lindner, Petra" sort="Lindner, Petra" uniqKey="Lindner P" first="Petra" last="Lindner">Petra Lindner</name><name sortKey="Motz, Manfred" sort="Motz, Manfred" uniqKey="Motz M" first="Manfred" last="Motz">Manfred Motz</name><name sortKey="Wolf, Hans" sort="Wolf, Hans" uniqKey="Wolf H" first="Hans" last="Wolf">Hans Wolf</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Jianguo Xu" sort="Jianguo Xu" uniqKey="Jianguo Xu" last="Jianguo Xu">JIANGUO XU</name></noRegion><name sortKey="Quanbi Zhao" sort="Quanbi Zhao" uniqKey="Quanbi Zhao" last="Quanbi Zhao">QUANBI ZHAO</name><name sortKey="Yiming Shao" sort="Yiming Shao" uniqKey="Yiming Shao" last="Yiming Shao">YIMING SHAO</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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