SrasV1, PascalFrancis, Corpus, bibRecord, 000286

A line immunoassay utilizing recombinant nucleocapsid proteins for detection of antibodies to human coronaviruses

Identifieur interne : 000286 ( PascalFrancis/Corpus ); précédent : 000285; suivant : 000287

A line immunoassay utilizing recombinant nucleocapsid proteins for detection of antibodies to human coronaviruses

Auteurs : Christian Lehmann ; Hans Wolf ; JIANGUO XU ; QUANBI ZHAO ; YIMING SHAO ; Manfred Motz ; Petra Lindner

Source :

Diagnostic microbiology and infectious disease [ 0732-8893 ] ; 2008.

RBID : Pascal:08-0243387

Descripteurs français

Pascal (Inist)
- Homme, Coronavirus, Protéine recombinante, Nucléocapside, Détection, Anticorps, Prévalence, Sérologie, Réaction croisée, Microbiologie, Infection.

English descriptors

KwdEn :
- Antibody, Coronavirus, Cross reaction, Detection, Human, Infection, Microbiology, Nucleocapsid, Prevalence, Recombinant protein, Serology.

Abstract

Most coronaviruses infecting humans cause mild diseases, whereas severe acute respiratory syndrome (SARS)-associated coronavirus is an extremely dangerous pathogen. Here, we report the development of a serologic assay for detection of antibodies to human coronaviruses (HCoVs) based on recombinant nucleocapsid (N) proteins of all known pathogenic strains (229E, NL63, OC43, HKU1, SARS). The novel immunoassay is highly useful for epidemiologic surveys, where use of nucleic acid diagnostics often is limited. Purified recombinant antigens were immobilized on nitrocellulose membranes and applied in a line immunoassay, which allows rapid detection of antibodies to 5 different HCoVs in a single experiment. For assay evaluation, serum samples from persons infected with 229E or OC43 (acute/convalescent), recovered SARS patients and healthy donors were analyzed. Screening for nucleocapsid (N)-specific immunoglobulin G (IgG) in convalescent sera reached 100% sensitivity. With this new technique, we found that recently identified NL63 and HKU1 contribute significantly to the overall spectrum of coronavirus infections. Possibly, cross-reactive antibody responses were observed using 229E and OC43 serum pairs. However, the potential of this assay could clearly be demonstrated employing SARS-positive serum samples, where nonspecific binding to nucleocapsids of other HCoVs was not observed. This coronavirus strain-specific line immunoassay represents a powerful tool for serologic diagnostics.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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Format Inist (serveur)

NO :	PASCAL 08-0243387 INIST
ET :	A line immunoassay utilizing recombinant nucleocapsid proteins for detection of antibodies to human coronaviruses
AU :	LEHMANN (Christian); WOLF (Hans); JIANGUO XU; QUANBI ZHAO; YIMING SHAO; MOTZ (Manfred); LINDNER (Petra)
AF :	Institute for Medical Microbiology and Hygiene, University of Regensburg/93053 Regensburg/Allemagne (1 aut., 2 aut., 7 aut.); Chinese Center for Disease Control and Prevention (China CDC)/Beijing/Chine (3 aut., 4 aut., 5 aut.); Mikrogen GmbH/82061 Neuried/Allemagne (6 aut.)
DT :	Publication en série; Niveau analytique
SO :	Diagnostic microbiology and infectious disease; ISSN 0732-8893; Coden DMIDDZ; Etats-Unis; Da. 2008; Vol. 61; No. 1; Pp. 40-48; Bibl. 1 p.1/4
LA :	Anglais
EA :	Most coronaviruses infecting humans cause mild diseases, whereas severe acute respiratory syndrome (SARS)-associated coronavirus is an extremely dangerous pathogen. Here, we report the development of a serologic assay for detection of antibodies to human coronaviruses (HCoVs) based on recombinant nucleocapsid (N) proteins of all known pathogenic strains (229E, NL63, OC43, HKU1, SARS). The novel immunoassay is highly useful for epidemiologic surveys, where use of nucleic acid diagnostics often is limited. Purified recombinant antigens were immobilized on nitrocellulose membranes and applied in a line immunoassay, which allows rapid detection of antibodies to 5 different HCoVs in a single experiment. For assay evaluation, serum samples from persons infected with 229E or OC43 (acute/convalescent), recovered SARS patients and healthy donors were analyzed. Screening for nucleocapsid (N)-specific immunoglobulin G (IgG) in convalescent sera reached 100% sensitivity. With this new technique, we found that recently identified NL63 and HKU1 contribute significantly to the overall spectrum of coronavirus infections. Possibly, cross-reactive antibody responses were observed using 229E and OC43 serum pairs. However, the potential of this assay could clearly be demonstrated employing SARS-positive serum samples, where nonspecific binding to nucleocapsids of other HCoVs was not observed. This coronavirus strain-specific line immunoassay represents a powerful tool for serologic diagnostics.
CC :	002A05C10; 002B05
FD :	Homme; Coronavirus; Protéine recombinante; Nucléocapside; Détection; Anticorps; Prévalence; Sérologie; Réaction croisée; Microbiologie; Infection
FG :	Coronaviridae; Nidovirales; Virus; Epidémiologie
ED :	Human; Coronavirus; Recombinant protein; Nucleocapsid; Detection; Antibody; Prevalence; Serology; Cross reaction; Microbiology; Infection
EG :	Coronaviridae; Nidovirales; Virus; Epidemiology
SD :	Hombre; Coronavirus; Proteína recombinante; Nucleocápside; Detección; Anticuerpo; Prevalencia; Serología; Reacción cruzada; Microbiología; Infección
LO :	INIST-20217.354000195859010060
ID :	08-0243387

Links to Exploration step

Pascal:08-0243387

Le document en format XML

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<front><div type="abstract" xml:lang="en">Most coronaviruses infecting humans cause mild diseases, whereas severe acute respiratory syndrome (SARS)-associated coronavirus is an extremely dangerous pathogen. Here, we report the development of a serologic assay for detection of antibodies to human coronaviruses (HCoVs) based on recombinant nucleocapsid (N) proteins of all known pathogenic strains (229E, NL63, OC43, HKU1, SARS). The novel immunoassay is highly useful for epidemiologic surveys, where use of nucleic acid diagnostics often is limited. Purified recombinant antigens were immobilized on nitrocellulose membranes and applied in a line immunoassay, which allows rapid detection of antibodies to 5 different HCoVs in a single experiment. For assay evaluation, serum samples from persons infected with 229E or OC43 (acute/convalescent), recovered SARS patients and healthy donors were analyzed. Screening for nucleocapsid (N)-specific immunoglobulin G (IgG) in convalescent sera reached 100% sensitivity. With this new technique, we found that recently identified NL63 and HKU1 contribute significantly to the overall spectrum of coronavirus infections. Possibly, cross-reactive antibody responses were observed using 229E and OC43 serum pairs. However, the potential of this assay could clearly be demonstrated employing SARS-positive serum samples, where nonspecific binding to nucleocapsids of other HCoVs was not observed. This coronavirus strain-specific line immunoassay represents a powerful tool for serologic diagnostics.</div>
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<server><NO>PASCAL 08-0243387 INIST</NO>
<ET>A line immunoassay utilizing recombinant nucleocapsid proteins for detection of antibodies to human coronaviruses</ET>
<AU>LEHMANN (Christian); WOLF (Hans); JIANGUO XU; QUANBI ZHAO; YIMING SHAO; MOTZ (Manfred); LINDNER (Petra)</AU>
<AF>Institute for Medical Microbiology and Hygiene, University of Regensburg/93053 Regensburg/Allemagne (1 aut., 2 aut., 7 aut.); Chinese Center for Disease Control and Prevention (China CDC)/Beijing/Chine (3 aut., 4 aut., 5 aut.); Mikrogen GmbH/82061 Neuried/Allemagne (6 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Diagnostic microbiology and infectious disease; ISSN 0732-8893; Coden DMIDDZ; Etats-Unis; Da. 2008; Vol. 61; No. 1; Pp. 40-48; Bibl. 1 p.1/4</SO>
<LA>Anglais</LA>
<EA>Most coronaviruses infecting humans cause mild diseases, whereas severe acute respiratory syndrome (SARS)-associated coronavirus is an extremely dangerous pathogen. Here, we report the development of a serologic assay for detection of antibodies to human coronaviruses (HCoVs) based on recombinant nucleocapsid (N) proteins of all known pathogenic strains (229E, NL63, OC43, HKU1, SARS). The novel immunoassay is highly useful for epidemiologic surveys, where use of nucleic acid diagnostics often is limited. Purified recombinant antigens were immobilized on nitrocellulose membranes and applied in a line immunoassay, which allows rapid detection of antibodies to 5 different HCoVs in a single experiment. For assay evaluation, serum samples from persons infected with 229E or OC43 (acute/convalescent), recovered SARS patients and healthy donors were analyzed. Screening for nucleocapsid (N)-specific immunoglobulin G (IgG) in convalescent sera reached 100% sensitivity. With this new technique, we found that recently identified NL63 and HKU1 contribute significantly to the overall spectrum of coronavirus infections. Possibly, cross-reactive antibody responses were observed using 229E and OC43 serum pairs. However, the potential of this assay could clearly be demonstrated employing SARS-positive serum samples, where nonspecific binding to nucleocapsids of other HCoVs was not observed. This coronavirus strain-specific line immunoassay represents a powerful tool for serologic diagnostics.</EA>
<CC>002A05C10; 002B05</CC>
<FD>Homme; Coronavirus; Protéine recombinante; Nucléocapside; Détection; Anticorps; Prévalence; Sérologie; Réaction croisée; Microbiologie; Infection</FD>
<FG>Coronaviridae; Nidovirales; Virus; Epidémiologie</FG>
<ED>Human; Coronavirus; Recombinant protein; Nucleocapsid; Detection; Antibody; Prevalence; Serology; Cross reaction; Microbiology; Infection</ED>
<EG>Coronaviridae; Nidovirales; Virus; Epidemiology</EG>
<SD>Hombre; Coronavirus; Proteína recombinante; Nucleocápside; Detección; Anticuerpo; Prevalencia; Serología; Reacción cruzada; Microbiología; Infección</SD>
<LO>INIST-20217.354000195859010060</LO>
<ID>08-0243387</ID>
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A line immunoassay utilizing recombinant nucleocapsid proteins for detection of antibodies to human coronaviruses

A line immunoassay utilizing recombinant nucleocapsid proteins for detection of antibodies to human coronaviruses

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