The use of Armored RNA as a multi-purpose internal control for RT-PCR
Identifieur interne : 003461 ( Main/Exploration ); précédent : 003460; suivant : 003462The use of Armored RNA as a multi-purpose internal control for RT-PCR
Auteurs : Jeffery Stevenson [États-Unis] ; Weston Hymas [États-Unis] ; David Hillyard [États-Unis]Source :
- Journal of virological methods [ 0166-0934 ] ; 2008.
Descripteurs français
- KwdFr :
- MESH :
- diagnostic : Maladies virales.
- génétique : Amorces ADN, Levivirus.
- normes : RT-PCR.
- virologie : Infections de l'appareil respiratoire.
- Pascal (Inist)
English descriptors
- KwdEn :
- DNA Primers (genetics), Humans, Levivirus (genetics), Method, Microbiology, Phage MS2, RNA (chemistry), Reference Standards, Respiratory Tract Infections (virology), Reverse Transcriptase Polymerase Chain Reaction (methods), Reverse Transcriptase Polymerase Chain Reaction (standards), Reverse transcription polymerase chain reaction, Virology, Virus Diseases (diagnosis).
- MESH :
- chemical , chemistry : RNA.
- chemical , genetics : DNA Primers.
- diagnosis : Virus Diseases.
- genetics : Levivirus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- standards : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Respiratory Tract Infections.
- Humans, Reference Standards.
Abstract
Real time reverse transcriptase-PCR (RT-PCR) is now used commonly for the detection of viral pathogens in respiratory samples. However, due to potential inhibition of the RT-PCR or inefficient extraction, this sample type can present significant challenges to accurate patient testing. The goal of this study was to create an internal control to be multiplexed in a real time RT-PCR assay for detecting a viral target in respiratory samples. This report describes an Armored RNA (aRNA) internal control developed originally to be multiplexed in a real time RT-PCR assay for detecting SARS-associated Coronavirus, but can be incorporated into any RT-PCR assay. The internal control primers and probe target a region in the coat protein gene of the E. coil F-specific bacteriophage ms2, which is contained within the aRNA.
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en">Real time reverse transcriptase-PCR (RT-PCR) is now used commonly for the detection of viral pathogens in respiratory samples. However, due to potential inhibition of the RT-PCR or inefficient extraction, this sample type can present significant challenges to accurate patient testing. The goal of this study was to create an internal control to be multiplexed in a real time RT-PCR assay for detecting a viral target in respiratory samples. This report describes an Armored RNA (aRNA) internal control developed originally to be multiplexed in a real time RT-PCR assay for detecting SARS-associated Coronavirus, but can be incorporated into any RT-PCR assay. The internal control primers and probe target a region in the coat protein gene of the E. coil F-specific bacteriophage ms2, which is contained within the aRNA.</div>
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