SrasV1, Main, Exploration, bibRecord, 003461

The use of Armored RNA as a multi-purpose internal control for RT-PCR

Identifieur interne : 003461 ( Main/Exploration ); précédent : 003460; suivant : 003462

The use of Armored RNA as a multi-purpose internal control for RT-PCR

Auteurs : Jeffery Stevenson [États-Unis] ; Weston Hymas [États-Unis] ; David Hillyard [États-Unis]

Source :

Journal of virological methods [ 0166-0934 ] ; 2008.

RBID : Pascal:08-0264007

Descripteurs français

KwdFr :
- ARN (), Amorces ADN (génétique), Humains, Infections de l'appareil respiratoire (virologie), Levivirus (génétique), Maladies virales (diagnostic), Normes de référence, RT-PCR (), RT-PCR (normes).
MESH :
- diagnostic : Maladies virales.
- génétique : Amorces ADN, Levivirus.
- normes : RT-PCR.
- virologie : Infections de l'appareil respiratoire.
Pascal (Inist)
- ARN, Bactériophage MS2, Humains, Normes de référence, RT-PCR, Réaction chaîne polymérase RT, Microbiologie, Méthode, Virologie.

English descriptors

KwdEn :
- DNA Primers (genetics), Humans, Levivirus (genetics), Method, Microbiology, Phage MS2, RNA (chemistry), Reference Standards, Respiratory Tract Infections (virology), Reverse Transcriptase Polymerase Chain Reaction (methods), Reverse Transcriptase Polymerase Chain Reaction (standards), Reverse transcription polymerase chain reaction, Virology, Virus Diseases (diagnosis).
MESH :
- chemical , chemistry : RNA.
- chemical , genetics : DNA Primers.
- diagnosis : Virus Diseases.
- genetics : Levivirus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- standards : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Respiratory Tract Infections.
- Humans, Reference Standards.

Abstract

Real time reverse transcriptase-PCR (RT-PCR) is now used commonly for the detection of viral pathogens in respiratory samples. However, due to potential inhibition of the RT-PCR or inefficient extraction, this sample type can present significant challenges to accurate patient testing. The goal of this study was to create an internal control to be multiplexed in a real time RT-PCR assay for detecting a viral target in respiratory samples. This report describes an Armored RNA (aRNA) internal control developed originally to be multiplexed in a real time RT-PCR assay for detecting SARS-associated Coronavirus, but can be incorporated into any RT-PCR assay. The internal control primers and probe target a region in the coat protein gene of the E. coil F-specific bacteriophage ms2, which is contained within the aRNA.

Affiliations:

Le document en format XML

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<front><div type="abstract" xml:lang="en">Real time reverse transcriptase-PCR (RT-PCR) is now used commonly for the detection of viral pathogens in respiratory samples. However, due to potential inhibition of the RT-PCR or inefficient extraction, this sample type can present significant challenges to accurate patient testing. The goal of this study was to create an internal control to be multiplexed in a real time RT-PCR assay for detecting a viral target in respiratory samples. This report describes an Armored RNA (aRNA) internal control developed originally to be multiplexed in a real time RT-PCR assay for detecting SARS-associated Coronavirus, but can be incorporated into any RT-PCR assay. The internal control primers and probe target a region in the coat protein gene of the E. coil F-specific bacteriophage ms2, which is contained within the aRNA.</div>
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The use of Armored RNA as a multi-purpose internal control for RT-PCR

The use of Armored RNA as a multi-purpose internal control for RT-PCR

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

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