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The use of Armored RNA as a multi-purpose internal control for RT-PCR.

Identifieur interne : 001B74 ( PubMed/Curation ); précédent : 001B73; suivant : 001B75

The use of Armored RNA as a multi-purpose internal control for RT-PCR.

Auteurs : Jeffery Stevenson [États-Unis] ; Weston Hymas ; David Hillyard

Source :

Journal of virological methods [ 0166-0934 ] ; 2008.

RBID : pubmed:18395804

Descripteurs français

KwdFr :
- ARN (), Amorces ADN (génétique), Humains, Infections de l'appareil respiratoire (virologie), Levivirus (génétique), Maladies virales (diagnostic), Normes de référence, RT-PCR (), RT-PCR (normes).
MESH :
- diagnostic : Maladies virales.
- génétique : Amorces ADN, Levivirus.
- normes : RT-PCR.
- virologie : Infections de l'appareil respiratoire.
- ARN, Humains, Normes de référence, RT-PCR.

English descriptors

KwdEn :
- DNA Primers (genetics), Humans, Levivirus (genetics), RNA (chemistry), Reference Standards, Respiratory Tract Infections (virology), Reverse Transcriptase Polymerase Chain Reaction (methods), Reverse Transcriptase Polymerase Chain Reaction (standards), Virus Diseases (diagnosis).
MESH :
- chemical , chemistry : RNA.
- chemical , genetics : DNA Primers.
- diagnosis : Virus Diseases.
- genetics : Levivirus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- standards : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Respiratory Tract Infections.
- Humans, Reference Standards.

Abstract

Real time reverse transcriptase-PCR (RT-PCR) is now used commonly for the detection of viral pathogens in respiratory samples. However, due to potential inhibition of the RT-PCR or inefficient extraction, this sample type can present significant challenges to accurate patient testing. The goal of this study was to create an internal control to be multiplexed in a real time RT-PCR assay for detecting a viral target in respiratory samples. This report describes an Armored RNA (aRNA) internal control developed originally to be multiplexed in a real time RT-PCR assay for detecting SARS-associated Coronavirus, but can be incorporated into any RT-PCR assay. The internal control primers and probe target a region in the coat protein gene of the E. coli F-specific bacteriophage ms2, which is contained within the aRNA.

DOI: 10.1016/j.jviromet.2008.02.007
PubMed: 18395804

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Le document en format XML

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<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Associated Regional and University Pathologists, Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108</wicri:regionArea>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA Primers (genetics)</term>
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<term>Infections de l'appareil respiratoire (virologie)</term>
<term>Levivirus (génétique)</term>
<term>Maladies virales (diagnostic)</term>
<term>Normes de référence</term>
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<front><div type="abstract" xml:lang="en">Real time reverse transcriptase-PCR (RT-PCR) is now used commonly for the detection of viral pathogens in respiratory samples. However, due to potential inhibition of the RT-PCR or inefficient extraction, this sample type can present significant challenges to accurate patient testing. The goal of this study was to create an internal control to be multiplexed in a real time RT-PCR assay for detecting a viral target in respiratory samples. This report describes an Armored RNA (aRNA) internal control developed originally to be multiplexed in a real time RT-PCR assay for detecting SARS-associated Coronavirus, but can be incorporated into any RT-PCR assay. The internal control primers and probe target a region in the coat protein gene of the E. coli F-specific bacteriophage ms2, which is contained within the aRNA.</div>
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The use of Armored RNA as a multi-purpose internal control for RT-PCR.

The use of Armored RNA as a multi-purpose internal control for RT-PCR.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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Pour générer des pages wiki