The use of Armored RNA as a multi-purpose internal control for RT-PCR.
Identifieur interne : 001B74 ( PubMed/Curation ); précédent : 001B73; suivant : 001B75The use of Armored RNA as a multi-purpose internal control for RT-PCR.
Auteurs : Jeffery Stevenson [États-Unis] ; Weston Hymas ; David HillyardSource :
- Journal of virological methods [ 0166-0934 ] ; 2008.
Descripteurs français
- KwdFr :
- MESH :
- diagnostic : Maladies virales.
- génétique : Amorces ADN, Levivirus.
- normes : RT-PCR.
- virologie : Infections de l'appareil respiratoire.
- ARN, Humains, Normes de référence, RT-PCR.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : RNA.
- chemical , genetics : DNA Primers.
- diagnosis : Virus Diseases.
- genetics : Levivirus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- standards : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Respiratory Tract Infections.
- Humans, Reference Standards.
Abstract
Real time reverse transcriptase-PCR (RT-PCR) is now used commonly for the detection of viral pathogens in respiratory samples. However, due to potential inhibition of the RT-PCR or inefficient extraction, this sample type can present significant challenges to accurate patient testing. The goal of this study was to create an internal control to be multiplexed in a real time RT-PCR assay for detecting a viral target in respiratory samples. This report describes an Armored RNA (aRNA) internal control developed originally to be multiplexed in a real time RT-PCR assay for detecting SARS-associated Coronavirus, but can be incorporated into any RT-PCR assay. The internal control primers and probe target a region in the coat protein gene of the E. coli F-specific bacteriophage ms2, which is contained within the aRNA.
DOI: 10.1016/j.jviromet.2008.02.007
PubMed: 18395804
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA Primers (genetics)</term>
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<term>Infections de l'appareil respiratoire (virologie)</term>
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<front><div type="abstract" xml:lang="en">Real time reverse transcriptase-PCR (RT-PCR) is now used commonly for the detection of viral pathogens in respiratory samples. However, due to potential inhibition of the RT-PCR or inefficient extraction, this sample type can present significant challenges to accurate patient testing. The goal of this study was to create an internal control to be multiplexed in a real time RT-PCR assay for detecting a viral target in respiratory samples. This report describes an Armored RNA (aRNA) internal control developed originally to be multiplexed in a real time RT-PCR assay for detecting SARS-associated Coronavirus, but can be incorporated into any RT-PCR assay. The internal control primers and probe target a region in the coat protein gene of the E. coli F-specific bacteriophage ms2, which is contained within the aRNA.</div>
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