SrasV1, Main, Exploration, bibRecord, 002E96

Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development

Identifieur interne : 002E96 ( Main/Exploration ); précédent : 002E95; suivant : 002E97

Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development

Auteurs : HONGYUAN SHEN [Singapour] ; SHOU GUO FANG [Singapour] ; BO CHEN [Singapour] ; GUANG CHEN [Singapour] ; Felicia P. L. Tay [Singapour] ; DING XIANG LIU [Singapour]

Source :

Journal of virological methods [ 0166-0934 ] ; 2009.

RBID : Pascal:09-0319418

Descripteurs français

Pascal (Inist)
- Virus bronchite infectieuse aviaire, Vecteur, Gène, Vaccin, Génétique, Microbiologie, Méthode, Virologie, Bronchite infectieuse aviaire.
Wicri :
- topic : Vaccin, Génétique.

English descriptors

KwdEn :
- Avian infectious bronchitis, Avian infectious bronchitis virus, Gene, Genetics, Method, Microbiology, Vaccine, Vector, Virology.

Abstract

Manipulation of the coronavirus genome to accommodate and express foreign genes is an attractive approach for gene delivery and vaccine development. By using an infectious cloning system developed recently for the avian coronavirus infectious bronchitis virus (IBV), the enhanced green fluorescent protein (EGFP) gene, the firefly luciferase gene and several host and viral genes (eIF3f, SARS ORF6, Dengue virus 1 core protein gene) were inserted into various positions of the IBV genome, and the effects on gene expression, virus recovery, and stability in cell culture were studied. Selected viruses were also inoculated into chicken embryos for studies of foreign gene expression at different tissue level. The results demonstrated the stability of recombinant viruses depends on the intrinsic properties of the foreign gene itself as well as the position at which the foreign genes were inserted. For unstable viruses, the loss of expression of the inserted genes was found to result from a large deletion of the inserted gene and even IBV backbone sequences. This represents a promising system for development of coronavirus-based gene delivery vectors and vaccines against coronavirus and other viral infections in chicken.

Affiliations:

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000202
to stream PascalFrancis, to step Curation: 000786
to stream PascalFrancis, to step Checkpoint: 000173
to stream Main, to step Merge: 002F50
to stream Main, to step Curation: 002E96

Le document en format XML

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<front><div type="abstract" xml:lang="en">Manipulation of the coronavirus genome to accommodate and express foreign genes is an attractive approach for gene delivery and vaccine development. By using an infectious cloning system developed recently for the avian coronavirus infectious bronchitis virus (IBV), the enhanced green fluorescent protein (EGFP) gene, the firefly luciferase gene and several host and viral genes (eIF3f, SARS ORF6, Dengue virus 1 core protein gene) were inserted into various positions of the IBV genome, and the effects on gene expression, virus recovery, and stability in cell culture were studied. Selected viruses were also inoculated into chicken embryos for studies of foreign gene expression at different tissue level. The results demonstrated the stability of recombinant viruses depends on the intrinsic properties of the foreign gene itself as well as the position at which the foreign genes were inserted. For unstable viruses, the loss of expression of the inserted genes was found to result from a large deletion of the inserted gene and even IBV backbone sequences. This represents a promising system for development of coronavirus-based gene delivery vectors and vaccines against coronavirus and other viral infections in chicken.</div>
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Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development

Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri