The 7a accessory protein of severe acute respiratory syndrome coronavirus acts as an RNA silencing suppressor.
Identifieur interne : 002430 ( Main/Exploration ); précédent : 002429; suivant : 002431The 7a accessory protein of severe acute respiratory syndrome coronavirus acts as an RNA silencing suppressor.
Auteurs : Sumona Karjee [Inde] ; Ankita Minhas ; Vikas Sood ; Sanket S. Ponia ; Akhil C. Banerjea ; Vincent T K. Chow ; Sunil K. Mukherjee ; Sunil K. LalSource :
- Journal of virology [ 1098-5514 ] ; 2010.
Descripteurs français
- KwdFr :
- ARN viral (génétique), Gènes viraux, Humains, Interférence par ARN (physiologie), Lignée cellulaire, Petit ARN interférent (génétique), Protéines de la matrice virale (), Protéines de la matrice virale (génétique), Protéines de la matrice virale (physiologie), Suppression génétique, Virus des plantes (génétique), Virus des plantes (physiologie), Virus du SRAS (génétique), Virus du SRAS (pathogénicité), Virus du SRAS (physiologie), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (virologie).
- MESH :
- génétique : ARN viral, Petit ARN interférent, Protéines de la matrice virale, Virus des plantes, Virus du SRAS, Végétaux génétiquement modifiés.
- pathogénicité : Virus du SRAS.
- physiologie : Interférence par ARN, Protéines de la matrice virale, Virus des plantes, Virus du SRAS.
- virologie : Végétaux génétiquement modifiés.
- Gènes viraux, Humains, Lignée cellulaire, Protéines de la matrice virale, Suppression génétique.
English descriptors
- KwdEn :
- Cell Line, Genes, Viral, Humans, Plant Viruses (genetics), Plant Viruses (physiology), Plants, Genetically Modified (genetics), Plants, Genetically Modified (virology), RNA Interference (physiology), RNA, Small Interfering (genetics), RNA, Viral (genetics), SARS Virus (genetics), SARS Virus (pathogenicity), SARS Virus (physiology), Suppression, Genetic, Viral Matrix Proteins (chemistry), Viral Matrix Proteins (genetics), Viral Matrix Proteins (physiology).
- MESH :
- chemical , chemistry : Viral Matrix Proteins.
- chemical , genetics : RNA, Small Interfering, RNA, Viral, Viral Matrix Proteins.
- genetics : Plant Viruses, Plants, Genetically Modified, SARS Virus.
- pathogenicity : SARS Virus.
- physiology : Plant Viruses, RNA Interference, SARS Virus, Viral Matrix Proteins.
- virology : Plants, Genetically Modified.
- Cell Line, Genes, Viral, Humans, Suppression, Genetic.
Abstract
RNA silencing suppressors (RSSs) are well studied for plant viruses but are not well defined to date for animal viruses. Here, we have identified an RSS from a medically important positive-sense mammalian virus, Severe acute respiratory syndrome coronavirus. The viral 7a accessory protein suppressed both transgene and virus-induced gene silencing by reducing the levels of small interfering RNA (siRNA). The suppression of silencing was analyzed by two independent assays, and the middle region (amino acids [aa] 32 to 89) of 7a was responsible for suppression. Finally, the RNA suppression property and the enhancement of heterologous replicon activity by the 7a protein were confirmed for animal cell lines.
DOI: 10.1128/JVI.00748-10
PubMed: 20631126
Affiliations:
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Le document en format XML
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Ponia</name></author><author><name sortKey="Banerjea, Akhil C" sort="Banerjea, Akhil C" uniqKey="Banerjea A" first="Akhil C" last="Banerjea">Akhil C. Banerjea</name></author><author><name sortKey="Chow, Vincent T K" sort="Chow, Vincent T K" uniqKey="Chow V" first="Vincent T K" last="Chow">Vincent T K. Chow</name></author><author><name sortKey="Mukherjee, Sunil K" sort="Mukherjee, Sunil K" uniqKey="Mukherjee S" first="Sunil K" last="Mukherjee">Sunil K. Mukherjee</name></author><author><name sortKey="Lal, Sunil K" sort="Lal, Sunil K" uniqKey="Lal S" first="Sunil K" last="Lal">Sunil K. 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Here, we have identified an RSS from a medically important positive-sense mammalian virus, Severe acute respiratory syndrome coronavirus. The viral 7a accessory protein suppressed both transgene and virus-induced gene silencing by reducing the levels of small interfering RNA (siRNA). The suppression of silencing was analyzed by two independent assays, and the middle region (amino acids [aa] 32 to 89) of 7a was responsible for suppression. Finally, the RNA suppression property and the enhancement of heterologous replicon activity by the 7a protein were confirmed for animal cell lines.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Banerjea, Akhil C" sort="Banerjea, Akhil C" uniqKey="Banerjea A" first="Akhil C" last="Banerjea">Akhil C. Banerjea</name><name sortKey="Chow, Vincent T K" sort="Chow, Vincent T K" uniqKey="Chow V" first="Vincent T K" last="Chow">Vincent T K. Chow</name><name sortKey="Lal, Sunil K" sort="Lal, Sunil K" uniqKey="Lal S" first="Sunil K" last="Lal">Sunil K. Lal</name><name sortKey="Minhas, Ankita" sort="Minhas, Ankita" uniqKey="Minhas A" first="Ankita" last="Minhas">Ankita Minhas</name><name sortKey="Mukherjee, Sunil K" sort="Mukherjee, Sunil K" uniqKey="Mukherjee S" first="Sunil K" last="Mukherjee">Sunil K. Mukherjee</name><name sortKey="Ponia, Sanket S" sort="Ponia, Sanket S" uniqKey="Ponia S" first="Sanket S" last="Ponia">Sanket S. Ponia</name><name sortKey="Sood, Vikas" sort="Sood, Vikas" uniqKey="Sood V" first="Vikas" last="Sood">Vikas Sood</name></noCountry><country name="Inde"><noRegion><name sortKey="Karjee, Sumona" sort="Karjee, Sumona" uniqKey="Karjee S" first="Sumona" last="Karjee">Sumona Karjee</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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