Genomics and proteomics of mycobacteriophage patience, an accidental tourist in the Mycobacterium neighborhood.
Identifieur interne : 001644 ( Main/Exploration ); précédent : 001643; suivant : 001645Genomics and proteomics of mycobacteriophage patience, an accidental tourist in the Mycobacterium neighborhood.
Auteurs : Welkin H. Pope [États-Unis] ; Deborah Jacobs-Sera [États-Unis] ; Daniel A. Russell [États-Unis] ; Daniel H F. Rubin [États-Unis] ; Afsana Kajee [Afrique du Sud] ; Zama N P. Msibi [Afrique du Sud] ; Michelle H. Larsen [États-Unis] ; William R. Jacobs [États-Unis] ; Jeffrey G. Lawrence [États-Unis] ; Roger W. Hendrix [États-Unis] ; Graham F. Hatfull [États-Unis]Source :
- mBio [ 2150-7511 ] ; 2014.
Descripteurs français
- KwdFr :
- Codon, Composition en bases nucléiques, Génome viral, Mycobacterium smegmatis (virologie), Mycobactériophages (), Mycobactériophages (génétique), Mycobactériophages (isolement et purification), Mycobactériophages (physiologie), Protéines virales (analyse), Protéines virales (génétique), Protéome (analyse), Réplication virale, Spectrométrie de masse.
- MESH :
- analyse : Protéines virales, Protéome.
- génétique : Mycobactériophages, Protéines virales.
- isolement et purification : Mycobactériophages.
- physiologie : Mycobactériophages.
- virologie : Mycobacterium smegmatis.
- Codon, Composition en bases nucléiques, Génome viral, Mycobactériophages, Réplication virale, Spectrométrie de masse.
English descriptors
- KwdEn :
- Base Composition, Codon, Genome, Viral, Mass Spectrometry, Mycobacteriophages (chemistry), Mycobacteriophages (genetics), Mycobacteriophages (isolation & purification), Mycobacteriophages (physiology), Mycobacterium smegmatis (virology), Proteome (analysis), Viral Proteins (analysis), Viral Proteins (genetics), Virus Replication.
- MESH :
- chemical , analysis : Proteome, Viral Proteins.
- chemical , genetics : Viral Proteins.
- chemical : Codon.
- chemistry : Mycobacteriophages.
- genetics : Mycobacteriophages.
- isolation & purification : Mycobacteriophages.
- physiology : Mycobacteriophages.
- virology : Mycobacterium smegmatis.
- Base Composition, Genome, Viral, Mass Spectrometry, Virus Replication.
Abstract
Newly emerging human viruses such as Ebola virus, severe acute respiratory syndrome (SARS) virus, and HIV likely originate within an extant population of viruses in nonhuman hosts and acquire the ability to infect and cause disease in humans. Although several mechanisms preventing viral infection of particular hosts have been described, the mechanisms and constraints on viral host expansion are ill defined. We describe here mycobacteriophage Patience, a newly isolated phage recovered using Mycobacterium smegmatis mc(2)155 as a host. Patience has genomic features distinct from its M. smegmatis host, including a much lower GC content (50.3% versus 67.4%) and an abundance of codons that are rarely used in M. smegmatis. Nonetheless, it propagates well in M. smegmatis, and we demonstrate the use of mass spectrometry to show expression of over 75% of the predicted proteins, to identify new genes, to refine the genome annotation, and to estimate protein abundance. We propose that Patience evolved primarily among lower-GC hosts and that the disparities between its genomic profile and that of M. smegmatis presented only a minimal barrier to host expansion. Rapid adaptions to its new host include recent acquisition of higher-GC genes, expression of out-of-frame proteins within predicted genes, and codon selection among highly expressed genes toward the translational apparatus of its new host.
DOI: 10.1128/mBio.02145-14
PubMed: 25467442
Affiliations:
- Afrique du Sud, États-Unis
- Massachusetts, Pennsylvanie, État de New York
- Pittsburgh
- Université de Pittsburgh
Links toward previous steps (curation, corpus...)
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Le document en format XML
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Although several mechanisms preventing viral infection of particular hosts have been described, the mechanisms and constraints on viral host expansion are ill defined. We describe here mycobacteriophage Patience, a newly isolated phage recovered using Mycobacterium smegmatis mc(2)155 as a host. Patience has genomic features distinct from its M. smegmatis host, including a much lower GC content (50.3% versus 67.4%) and an abundance of codons that are rarely used in M. smegmatis. Nonetheless, it propagates well in M. smegmatis, and we demonstrate the use of mass spectrometry to show expression of over 75% of the predicted proteins, to identify new genes, to refine the genome annotation, and to estimate protein abundance. We propose that Patience evolved primarily among lower-GC hosts and that the disparities between its genomic profile and that of M. smegmatis presented only a minimal barrier to host expansion. Rapid adaptions to its new host include recent acquisition of higher-GC genes, expression of out-of-frame proteins within predicted genes, and codon selection among highly expressed genes toward the translational apparatus of its new host.</div></front></TEI><affiliations><list><country><li>Afrique du Sud</li><li>États-Unis</li></country><region><li>Massachusetts</li><li>Pennsylvanie</li><li>État de New York</li></region><settlement><li>Pittsburgh</li></settlement><orgName><li>Université de Pittsburgh</li></orgName></list><tree><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Pope, Welkin H" sort="Pope, Welkin H" uniqKey="Pope W" first="Welkin H" last="Pope">Welkin H. Pope</name></region><name sortKey="Hatfull, Graham F" sort="Hatfull, Graham F" uniqKey="Hatfull G" first="Graham F" last="Hatfull">Graham F. Hatfull</name><name sortKey="Hendrix, Roger W" sort="Hendrix, Roger W" uniqKey="Hendrix R" first="Roger W" last="Hendrix">Roger W. Hendrix</name><name sortKey="Jacobs Sera, Deborah" sort="Jacobs Sera, Deborah" uniqKey="Jacobs Sera D" first="Deborah" last="Jacobs-Sera">Deborah Jacobs-Sera</name><name sortKey="Jacobs, William R" sort="Jacobs, William R" uniqKey="Jacobs W" first="William R" last="Jacobs">William R. Jacobs</name><name sortKey="Larsen, Michelle H" sort="Larsen, Michelle H" uniqKey="Larsen M" first="Michelle H" last="Larsen">Michelle H. Larsen</name><name sortKey="Lawrence, Jeffrey G" sort="Lawrence, Jeffrey G" uniqKey="Lawrence J" first="Jeffrey G" last="Lawrence">Jeffrey G. Lawrence</name><name sortKey="Rubin, Daniel H F" sort="Rubin, Daniel H F" uniqKey="Rubin D" first="Daniel H F" last="Rubin">Daniel H F. Rubin</name><name sortKey="Russell, Daniel A" sort="Russell, Daniel A" uniqKey="Russell D" first="Daniel A" last="Russell">Daniel A. Russell</name></country><country name="Afrique du Sud"><noRegion><name sortKey="Kajee, Afsana" sort="Kajee, Afsana" uniqKey="Kajee A" first="Afsana" last="Kajee">Afsana Kajee</name></noRegion><name sortKey="Msibi, Zama N P" sort="Msibi, Zama N P" uniqKey="Msibi Z" first="Zama N P" last="Msibi">Zama N P. Msibi</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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