SrasV1, Ncbi, Merge, bibRecord, 002991

Genomics and proteomics of mycobacteriophage patience, an accidental tourist in the Mycobacterium neighborhood.

Identifieur interne : 002991 ( Ncbi/Merge ); précédent : 002990; suivant : 002992

Genomics and proteomics of mycobacteriophage patience, an accidental tourist in the Mycobacterium neighborhood.

Auteurs : Welkin H. Pope [États-Unis] ; Deborah Jacobs-Sera [États-Unis] ; Daniel A. Russell [États-Unis] ; Daniel H F. Rubin [États-Unis] ; Afsana Kajee [Afrique du Sud] ; Zama N P. Msibi [Afrique du Sud] ; Michelle H. Larsen [États-Unis] ; William R. Jacobs [États-Unis] ; Jeffrey G. Lawrence [États-Unis] ; Roger W. Hendrix [États-Unis] ; Graham F. Hatfull [États-Unis]

Source :

mBio [ 2150-7511 ] ; 2014.

RBID : pubmed:25467442

Descripteurs français

KwdFr :
- Codon, Composition en bases nucléiques, Génome viral, Mycobacterium smegmatis (virologie), Mycobactériophages (), Mycobactériophages (génétique), Mycobactériophages (isolement et purification), Mycobactériophages (physiologie), Protéines virales (analyse), Protéines virales (génétique), Protéome (analyse), Réplication virale, Spectrométrie de masse.
MESH :
- analyse : Protéines virales, Protéome.
- génétique : Mycobactériophages, Protéines virales.
- isolement et purification : Mycobactériophages.
- physiologie : Mycobactériophages.
- virologie : Mycobacterium smegmatis.
- Codon, Composition en bases nucléiques, Génome viral, Mycobactériophages, Réplication virale, Spectrométrie de masse.

English descriptors

KwdEn :
- Base Composition, Codon, Genome, Viral, Mass Spectrometry, Mycobacteriophages (chemistry), Mycobacteriophages (genetics), Mycobacteriophages (isolation & purification), Mycobacteriophages (physiology), Mycobacterium smegmatis (virology), Proteome (analysis), Viral Proteins (analysis), Viral Proteins (genetics), Virus Replication.
MESH :
- chemical , analysis : Proteome, Viral Proteins.
- chemical , genetics : Viral Proteins.
- chemical : Codon.
- chemistry : Mycobacteriophages.
- genetics : Mycobacteriophages.
- isolation & purification : Mycobacteriophages.
- physiology : Mycobacteriophages.
- virology : Mycobacterium smegmatis.
- Base Composition, Genome, Viral, Mass Spectrometry, Virus Replication.

Abstract

Newly emerging human viruses such as Ebola virus, severe acute respiratory syndrome (SARS) virus, and HIV likely originate within an extant population of viruses in nonhuman hosts and acquire the ability to infect and cause disease in humans. Although several mechanisms preventing viral infection of particular hosts have been described, the mechanisms and constraints on viral host expansion are ill defined. We describe here mycobacteriophage Patience, a newly isolated phage recovered using Mycobacterium smegmatis mc(2)155 as a host. Patience has genomic features distinct from its M. smegmatis host, including a much lower GC content (50.3% versus 67.4%) and an abundance of codons that are rarely used in M. smegmatis. Nonetheless, it propagates well in M. smegmatis, and we demonstrate the use of mass spectrometry to show expression of over 75% of the predicted proteins, to identify new genes, to refine the genome annotation, and to estimate protein abundance. We propose that Patience evolved primarily among lower-GC hosts and that the disparities between its genomic profile and that of M. smegmatis presented only a minimal barrier to host expansion. Rapid adaptions to its new host include recent acquisition of higher-GC genes, expression of out-of-frame proteins within predicted genes, and codon selection among highly expressed genes toward the translational apparatus of its new host.

DOI: 10.1128/mBio.02145-14
PubMed: 25467442

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Newly emerging human viruses such as Ebola virus, severe acute respiratory syndrome (SARS) virus, and HIV likely originate within an extant population of viruses in nonhuman hosts and acquire the ability to infect and cause disease in humans. Although several mechanisms preventing viral infection of particular hosts have been described, the mechanisms and constraints on viral host expansion are ill defined. We describe here mycobacteriophage Patience, a newly isolated phage recovered using Mycobacterium smegmatis mc(2)155 as a host. Patience has genomic features distinct from its M. smegmatis host, including a much lower GC content (50.3% versus 67.4%) and an abundance of codons that are rarely used in M. smegmatis. Nonetheless, it propagates well in M. smegmatis, and we demonstrate the use of mass spectrometry to show expression of over 75% of the predicted proteins, to identify new genes, to refine the genome annotation, and to estimate protein abundance. We propose that Patience evolved primarily among lower-GC hosts and that the disparities between its genomic profile and that of M. smegmatis presented only a minimal barrier to host expansion. Rapid adaptions to its new host include recent acquisition of higher-GC genes, expression of out-of-frame proteins within predicted genes, and codon selection among highly expressed genes toward the translational apparatus of its new host.</div>
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<Abstract><AbstractText Label="UNLABELLED">Newly emerging human viruses such as Ebola virus, severe acute respiratory syndrome (SARS) virus, and HIV likely originate within an extant population of viruses in nonhuman hosts and acquire the ability to infect and cause disease in humans. Although several mechanisms preventing viral infection of particular hosts have been described, the mechanisms and constraints on viral host expansion are ill defined. We describe here mycobacteriophage Patience, a newly isolated phage recovered using Mycobacterium smegmatis mc(2)155 as a host. Patience has genomic features distinct from its M. smegmatis host, including a much lower GC content (50.3% versus 67.4%) and an abundance of codons that are rarely used in M. smegmatis. Nonetheless, it propagates well in M. smegmatis, and we demonstrate the use of mass spectrometry to show expression of over 75% of the predicted proteins, to identify new genes, to refine the genome annotation, and to estimate protein abundance. We propose that Patience evolved primarily among lower-GC hosts and that the disparities between its genomic profile and that of M. smegmatis presented only a minimal barrier to host expansion. Rapid adaptions to its new host include recent acquisition of higher-GC genes, expression of out-of-frame proteins within predicted genes, and codon selection among highly expressed genes toward the translational apparatus of its new host.</AbstractText>
<AbstractText Label="IMPORTANCE" NlmCategory="OBJECTIVE">The mycobacteriophage Patience genome has a notably lower GC content (50.3%) than its Mycobacterium smegmatis host (67.4%) and has markedly different codon usage biases. The viral genome has an abundance of codons that are rare in the host and are decoded by wobble tRNA pairing, although the phage grows well and expression of most of the genes is detected by mass spectrometry. Patience thus has the genomic profile of a virus that evolved primarily in one type of host genetic landscape (moderate-GC bacteria) but has found its way into a distinctly different high-GC environment. Although Patience genes are ill matched to the host expression apparatus, this is of little functional consequence and has not evidently imposed a barrier to migration across the microbial landscape. Interestingly, comparison of expression levels and codon usage profiles reveals evidence of codon selection as the genome evolves and adapts to its new environment.</AbstractText>
<CopyrightInformation>Copyright © 2014 Pope et al.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pope</LastName>
<ForeName>Welkin H</ForeName>
<Initials>WH</Initials>
<AffiliationInfo><Affiliation>Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Jacobs-Sera</LastName>
<ForeName>Deborah</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Russell</LastName>
<ForeName>Daniel A</ForeName>
<Initials>DA</Initials>
<AffiliationInfo><Affiliation>Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Rubin</LastName>
<ForeName>Daniel H F</ForeName>
<Initials>DH</Initials>
<AffiliationInfo><Affiliation>Harvard College, Cambridge, Massachusetts, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kajee</LastName>
<ForeName>Afsana</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>University of KwaZulu-Natal, School of Laboratory Medicine, College of Health Sciences, Inkosi Albert Luthuli Hospital, Durban, South Africa.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Msibi</LastName>
<ForeName>Zama N P</ForeName>
<Initials>ZN</Initials>
<AffiliationInfo><Affiliation>Department of Infection Prevention and Control, University of KwaZulu-Natal, Durban, South Africa.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Larsen</LastName>
<ForeName>Michelle H</ForeName>
<Initials>MH</Initials>
<AffiliationInfo><Affiliation>Department of Medicine, Albert Einstein College of Medicine, Bronx, New York, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Jacobs</LastName>
<ForeName>William R</ForeName>
<Initials>WR</Initials>
<Suffix>Jr</Suffix>
<AffiliationInfo><Affiliation>Howard Hughes Medical Institute, Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lawrence</LastName>
<ForeName>Jeffrey G</ForeName>
<Initials>JG</Initials>
<AffiliationInfo><Affiliation>Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hendrix</LastName>
<ForeName>Roger W</ForeName>
<Initials>RW</Initials>
<AffiliationInfo><Affiliation>Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hatfull</LastName>
<ForeName>Graham F</ForeName>
<Initials>GF</Initials>
<AffiliationInfo><Affiliation>Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA gfh@pitt.edu.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>UL1 TR000005</GrantID>
<Acronym>TR</Acronym>
<Agency>NCATS NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>GM093901</GrantID>
<Acronym>GM</Acronym>
<Agency>NIGMS NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>GM077548</GrantID>
<Acronym>GM</Acronym>
<Agency>NIGMS NIH HHS</Agency>
<Country>United States</Country>
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<Country>United States</Country>
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<Country>United States</Country>
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<Country>United States</Country>
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<Acronym>GM</Acronym>
<Agency>NIGMS NIH HHS</Agency>
<Country>United States</Country>
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<Acronym>GM</Acronym>
<Agency>NIGMS NIH HHS</Agency>
<Country>United States</Country>
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<Agency>NIAID NIH HHS</Agency>
<Country>United States</Country>
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<Acronym>GM</Acronym>
<Agency>NIGMS NIH HHS</Agency>
<Country>United States</Country>
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<PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType>
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<Month>12</Month>
<Day>02</Day>
</ArticleDate>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D003062">Codon</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D020543">Proteome</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance>
</Chemical>
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<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D001482" MajorTopicYN="N">Base Composition</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003062" MajorTopicYN="N">Codon</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016679" MajorTopicYN="Y">Genome, Viral</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013058" MajorTopicYN="N">Mass Spectrometry</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009160" MajorTopicYN="N">Mycobacteriophages</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020102" MajorTopicYN="N">Mycobacterium smegmatis</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020543" MajorTopicYN="N">Proteome</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName>
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<QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
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<MeshHeading><DescriptorName UI="D014779" MajorTopicYN="N">Virus Replication</DescriptorName>
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<ReferenceList><Reference><Citation>Genome Announc. 2013 Nov 27;1(6):null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24285655</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 1998 Jun 11;393(6685):537-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9634230</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Adv Virus Res. 2012;82:179-288</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22420855</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Adv Bioinformatics. 2009;:316936</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20150956</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Microbiology. 2008 May;154(Pt 5):1364-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18451045</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2012;7(3):e34052</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22470512</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2012 Dec 20;434(2):187-201</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23084079</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Microbiol. 2010;64:331-56</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20528690</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11928-33</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23812753</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1997 Mar 1;25(5):955-64</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9023104</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Microbiol. 2001 Jan;39(2):213-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11136444</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biochem Mol Biol. 2004 Jul 31;37(4):487-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15469738</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Theor Biol. 2000 Jul 7;205(1):167-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10860710</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Bioinformatics. 2007 Apr 15;23(8):1026-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17309896</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Genomics. 2011;12:374</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21787402</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Microbiol. 1993 Feb;7(3):395-405</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8459766</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2004;32(1):11-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14704338</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Microbiol. 1995 Sep;17(6):1045-56</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8594325</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 1997 Oct 1;16(19):5914-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9312049</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2012 Feb;86(4):2382-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22282335</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Biochem Sci. 2000 Feb;25(2):70-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10664586</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2005;33(4):1141-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15728743</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Microbiol. 2013 May;88(3):577-89</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23560716</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Microb Pathog. 1997 Jun;22(6):343-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9188089</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Genet. 2006 Jun;2(6):e92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16789831</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Mol Biol. 1998;101:145-73</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9921476</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Microbiol. 2010 Aug;77(3):672-86</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20545844</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):6022-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24711378</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2008;3(4):e2048</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18431505</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Bioinformatics. 2011;12:395</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21991981</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Infect Immun. 1985 Jul;49(1):32-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3924830</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2014 Mar;88(5):2461-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24335314</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Bacteriol. 2014 Oct;196(20):3589-97</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25092027</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Microbiol. 2009 Aug;73(3):367-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19555454</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W244-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15980461</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Microb Ecol. 2014 Feb;67(2):242-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24253663</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 2003 Apr 18;113(2):171-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12705866</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
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Genomics and proteomics of mycobacteriophage patience, an accidental tourist in the Mycobacterium neighborhood.

Genomics and proteomics of mycobacteriophage patience, an accidental tourist in the Mycobacterium neighborhood.

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