Ebola virus and severe acute respiratory syndrome coronavirus display late cell entry kinetics: evidence that transport to NPC1+ endolysosomes is a rate-defining step.
Identifieur interne : 001323 ( Main/Exploration ); précédent : 001322; suivant : 001324Ebola virus and severe acute respiratory syndrome coronavirus display late cell entry kinetics: evidence that transport to NPC1+ endolysosomes is a rate-defining step.
Auteurs : Rebecca M. Mingo [États-Unis] ; James A. Simmons [États-Unis] ; Charles J. Shoemaker [États-Unis] ; Elizabeth A. Nelson [États-Unis] ; Kathryn L. Schornberg [États-Unis] ; Ryan S. D'Souza [États-Unis] ; James E. Casanova [États-Unis] ; Judith M. White [États-Unis]Source :
- Journal of virology [ 1098-5514 ] ; 2015.
Descripteurs français
- KwdFr :
- Ebolavirus (physiologie), Endosomes (), Endosomes (virologie), Facteurs temps, Glycoprotéines membranaires (analyse), Humains, Lignée cellulaire, Lysosomes (), Lysosomes (virologie), Protéines de transport (analyse), Pénétration virale, Transport biologique, Virosomes (métabolisme), Virus du SRAS (physiologie).
- MESH :
- analyse : Glycoprotéines membranaires, Protéines de transport.
- métabolisme : Virosomes.
- physiologie : Ebolavirus, Virus du SRAS.
- virologie : Endosomes, Lysosomes.
- Endosomes, Facteurs temps, Humains, Lignée cellulaire, Lysosomes, Pénétration virale, Transport biologique.
English descriptors
- KwdEn :
- Biological Transport, Carrier Proteins (analysis), Cell Line, Ebolavirus (physiology), Endosomes (chemistry), Endosomes (virology), Humans, Lysosomes (chemistry), Lysosomes (virology), Membrane Glycoproteins (analysis), SARS Virus (physiology), Time Factors, Virosomes (metabolism), Virus Internalization.
- MESH :
- chemical , analysis : Carrier Proteins, Membrane Glycoproteins.
- chemistry : Endosomes, Lysosomes.
- chemical , metabolism : Virosomes.
- physiology : Ebolavirus, SARS Virus.
- virology : Endosomes, Lysosomes.
- Biological Transport, Cell Line, Humans, Time Factors, Virus Internalization.
Abstract
Ebola virus (EBOV) causes hemorrhagic fevers with high mortality rates. During cellular entry, the virus is internalized by macropinocytosis and trafficked through endosomes until fusion between the viral and an endosomal membrane is triggered, releasing the RNA genome into the cytoplasm. We found that while macropinocytotic uptake of filamentous EBOV viruslike particles (VLPs) expressing the EBOV glycoprotein (GP) occurs relatively quickly, VLPs only begin to enter the cytoplasm after a 30-min lag, considerably later than particles bearing the influenza hemagglutinin or GP from lymphocytic choriomeningitis virus, which enter through late endosomes (LE). For EBOV, the long lag is not due to the large size or unusual shape of EBOV filaments, the need to prime EBOV GP to the 19-kDa receptor-binding species, or a need for unusually low endosomal pH. In contrast, since we observed that EBOV entry occurs upon arrival in Niemann-Pick C1 (NPC1)-positive endolysosomes (LE/Lys), we propose that trafficking to LE/Lys is a key rate-defining step. Additional experiments revealed, unexpectedly, that severe acute respiratory syndrome (SARS) S-mediated entry also begins only after a 30-min lag. Furthermore, although SARS does not require NPC1 for entry, SARS entry also begins after colocalization with NPC1. Since the only endosomal requirement for SARS entry is cathepsin L activity, we tested and provide evidence that NPC1(+) LE/Lys have higher cathepsin L activity than LE, with no detectable activity in earlier endosomes. Our findings suggest that both EBOV and SARS traffic deep into the endocytic pathway for entry and that they do so to access higher cathepsin activity.
DOI: 10.1128/JVI.03398-14
PubMed: 25552710
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000E82
- to stream PubMed, to step Curation: 000E82
- to stream PubMed, to step Checkpoint: 000E22
- to stream Ncbi, to step Merge: 002A06
- to stream Ncbi, to step Curation: 002A06
- to stream Ncbi, to step Checkpoint: 002A06
- to stream Main, to step Merge: 001325
- to stream Main, to step Curation: 001323
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Ebola virus and severe acute respiratory syndrome coronavirus display late cell entry kinetics: evidence that transport to NPC1+ endolysosomes is a rate-defining step.</title><author><name sortKey="Mingo, Rebecca M" sort="Mingo, Rebecca M" uniqKey="Mingo R" first="Rebecca M" last="Mingo">Rebecca M. Mingo</name><affiliation wicri:level="2"><nlm:affiliation>Department of Cell Biology, University of Virginia, Charlottesville, Virginia, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Cell Biology, University of Virginia, Charlottesville, Virginia</wicri:regionArea><placeName><region type="state">Virginie</region></placeName></affiliation></author><author><name sortKey="Simmons, James A" sort="Simmons, James A" uniqKey="Simmons J" first="James A" last="Simmons">James A. Simmons</name><affiliation wicri:level="2"><nlm:affiliation>Department of Cell Biology, University of Virginia, Charlottesville, Virginia, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Cell Biology, University of Virginia, Charlottesville, Virginia</wicri:regionArea><placeName><region type="state">Virginie</region></placeName></affiliation></author><author><name sortKey="Shoemaker, Charles J" sort="Shoemaker, Charles J" uniqKey="Shoemaker C" first="Charles J" last="Shoemaker">Charles J. Shoemaker</name><affiliation wicri:level="2"><nlm:affiliation>Department of Cell Biology, University of Virginia, Charlottesville, Virginia, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Cell Biology, University of Virginia, Charlottesville, Virginia</wicri:regionArea><placeName><region type="state">Virginie</region></placeName></affiliation></author><author><name sortKey="Nelson, Elizabeth A" sort="Nelson, Elizabeth A" uniqKey="Nelson E" first="Elizabeth A" last="Nelson">Elizabeth A. Nelson</name><affiliation wicri:level="2"><nlm:affiliation>Department of Cell Biology, University of Virginia, Charlottesville, Virginia, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Cell Biology, University of Virginia, Charlottesville, Virginia</wicri:regionArea><placeName><region type="state">Virginie</region></placeName></affiliation></author><author><name sortKey="Schornberg, Kathryn L" sort="Schornberg, Kathryn L" uniqKey="Schornberg K" first="Kathryn L" last="Schornberg">Kathryn L. Schornberg</name><affiliation wicri:level="2"><nlm:affiliation>Department of Cell Biology, University of Virginia, Charlottesville, Virginia, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Cell Biology, University of Virginia, Charlottesville, Virginia</wicri:regionArea><placeName><region type="state">Virginie</region></placeName></affiliation></author><author><name sortKey="D Souza, Ryan S" sort="D Souza, Ryan S" uniqKey="D Souza R" first="Ryan S" last="D'Souza">Ryan S. D'Souza</name><affiliation wicri:level="2"><nlm:affiliation>Department of Cell Biology, University of Virginia, Charlottesville, Virginia, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Cell Biology, University of Virginia, Charlottesville, Virginia</wicri:regionArea><placeName><region type="state">Virginie</region></placeName></affiliation></author><author><name sortKey="Casanova, James E" sort="Casanova, James E" uniqKey="Casanova J" first="James E" last="Casanova">James E. Casanova</name><affiliation wicri:level="2"><nlm:affiliation>Department of Cell Biology, University of Virginia, Charlottesville, Virginia, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Cell Biology, University of Virginia, Charlottesville, Virginia</wicri:regionArea><placeName><region type="state">Virginie</region></placeName></affiliation></author><author><name sortKey="White, Judith M" sort="White, Judith M" uniqKey="White J" first="Judith M" last="White">Judith M. White</name><affiliation wicri:level="2"><nlm:affiliation>Department of Cell Biology, University of Virginia, Charlottesville, Virginia, USA jw7g@virginia.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Department of Cell Biology, University of Virginia, Charlottesville, Virginia</wicri:regionArea><placeName><region type="state">Virginie</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:25552710</idno><idno type="pmid">25552710</idno><idno type="doi">10.1128/JVI.03398-14</idno><idno type="wicri:Area/PubMed/Corpus">000E82</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000E82</idno><idno type="wicri:Area/PubMed/Curation">000E82</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000E82</idno><idno type="wicri:Area/PubMed/Checkpoint">000E22</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000E22</idno><idno type="wicri:Area/Ncbi/Merge">002A06</idno><idno type="wicri:Area/Ncbi/Curation">002A06</idno><idno type="wicri:Area/Ncbi/Checkpoint">002A06</idno><idno type="wicri:Area/Main/Merge">001325</idno><idno type="wicri:Area/Main/Curation">001323</idno><idno type="wicri:Area/Main/Exploration">001323</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Ebola virus and severe acute respiratory syndrome coronavirus display late cell entry kinetics: evidence that transport to NPC1+ endolysosomes is a rate-defining step.</title><author><name sortKey="Mingo, Rebecca M" sort="Mingo, Rebecca M" uniqKey="Mingo R" first="Rebecca M" last="Mingo">Rebecca M. Mingo</name><affiliation wicri:level="2"><nlm:affiliation>Department of Cell Biology, University of Virginia, Charlottesville, Virginia, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Cell Biology, University of Virginia, Charlottesville, Virginia</wicri:regionArea><placeName><region type="state">Virginie</region></placeName></affiliation></author><author><name sortKey="Simmons, James A" sort="Simmons, James A" uniqKey="Simmons J" first="James A" last="Simmons">James A. Simmons</name><affiliation wicri:level="2"><nlm:affiliation>Department of Cell Biology, University of Virginia, Charlottesville, Virginia, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Cell Biology, University of Virginia, Charlottesville, Virginia</wicri:regionArea><placeName><region type="state">Virginie</region></placeName></affiliation></author><author><name sortKey="Shoemaker, Charles J" sort="Shoemaker, Charles J" uniqKey="Shoemaker C" first="Charles J" last="Shoemaker">Charles J. Shoemaker</name><affiliation wicri:level="2"><nlm:affiliation>Department of Cell Biology, University of Virginia, Charlottesville, Virginia, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Cell Biology, University of Virginia, Charlottesville, Virginia</wicri:regionArea><placeName><region type="state">Virginie</region></placeName></affiliation></author><author><name sortKey="Nelson, Elizabeth A" sort="Nelson, Elizabeth A" uniqKey="Nelson E" first="Elizabeth A" last="Nelson">Elizabeth A. Nelson</name><affiliation wicri:level="2"><nlm:affiliation>Department of Cell Biology, University of Virginia, Charlottesville, Virginia, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Cell Biology, University of Virginia, Charlottesville, Virginia</wicri:regionArea><placeName><region type="state">Virginie</region></placeName></affiliation></author><author><name sortKey="Schornberg, Kathryn L" sort="Schornberg, Kathryn L" uniqKey="Schornberg K" first="Kathryn L" last="Schornberg">Kathryn L. Schornberg</name><affiliation wicri:level="2"><nlm:affiliation>Department of Cell Biology, University of Virginia, Charlottesville, Virginia, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Cell Biology, University of Virginia, Charlottesville, Virginia</wicri:regionArea><placeName><region type="state">Virginie</region></placeName></affiliation></author><author><name sortKey="D Souza, Ryan S" sort="D Souza, Ryan S" uniqKey="D Souza R" first="Ryan S" last="D'Souza">Ryan S. D'Souza</name><affiliation wicri:level="2"><nlm:affiliation>Department of Cell Biology, University of Virginia, Charlottesville, Virginia, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Cell Biology, University of Virginia, Charlottesville, Virginia</wicri:regionArea><placeName><region type="state">Virginie</region></placeName></affiliation></author><author><name sortKey="Casanova, James E" sort="Casanova, James E" uniqKey="Casanova J" first="James E" last="Casanova">James E. Casanova</name><affiliation wicri:level="2"><nlm:affiliation>Department of Cell Biology, University of Virginia, Charlottesville, Virginia, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Cell Biology, University of Virginia, Charlottesville, Virginia</wicri:regionArea><placeName><region type="state">Virginie</region></placeName></affiliation></author><author><name sortKey="White, Judith M" sort="White, Judith M" uniqKey="White J" first="Judith M" last="White">Judith M. White</name><affiliation wicri:level="2"><nlm:affiliation>Department of Cell Biology, University of Virginia, Charlottesville, Virginia, USA jw7g@virginia.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Department of Cell Biology, University of Virginia, Charlottesville, Virginia</wicri:regionArea><placeName><region type="state">Virginie</region></placeName></affiliation></author></analytic><series><title level="j">Journal of virology</title><idno type="eISSN">1098-5514</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biological Transport</term><term>Carrier Proteins (analysis)</term><term>Cell Line</term><term>Ebolavirus (physiology)</term><term>Endosomes (chemistry)</term><term>Endosomes (virology)</term><term>Humans</term><term>Lysosomes (chemistry)</term><term>Lysosomes (virology)</term><term>Membrane Glycoproteins (analysis)</term><term>SARS Virus (physiology)</term><term>Time Factors</term><term>Virosomes (metabolism)</term><term>Virus Internalization</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Ebolavirus (physiologie)</term><term>Endosomes ()</term><term>Endosomes (virologie)</term><term>Facteurs temps</term><term>Glycoprotéines membranaires (analyse)</term><term>Humains</term><term>Lignée cellulaire</term><term>Lysosomes ()</term><term>Lysosomes (virologie)</term><term>Protéines de transport (analyse)</term><term>Pénétration virale</term><term>Transport biologique</term><term>Virosomes (métabolisme)</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Carrier Proteins</term><term>Membrane Glycoproteins</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de transport</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Endosomes</term><term>Lysosomes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Virosomes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Virosomes</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Ebolavirus</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Ebolavirus</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Endosomes</term><term>Lysosomes</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Endosomes</term><term>Lysosomes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biological Transport</term><term>Cell Line</term><term>Humans</term><term>Time Factors</term><term>Virus Internalization</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Endosomes</term><term>Facteurs temps</term><term>Humains</term><term>Lignée cellulaire</term><term>Lysosomes</term><term>Pénétration virale</term><term>Transport biologique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Ebola virus (EBOV) causes hemorrhagic fevers with high mortality rates. During cellular entry, the virus is internalized by macropinocytosis and trafficked through endosomes until fusion between the viral and an endosomal membrane is triggered, releasing the RNA genome into the cytoplasm. We found that while macropinocytotic uptake of filamentous EBOV viruslike particles (VLPs) expressing the EBOV glycoprotein (GP) occurs relatively quickly, VLPs only begin to enter the cytoplasm after a 30-min lag, considerably later than particles bearing the influenza hemagglutinin or GP from lymphocytic choriomeningitis virus, which enter through late endosomes (LE). For EBOV, the long lag is not due to the large size or unusual shape of EBOV filaments, the need to prime EBOV GP to the 19-kDa receptor-binding species, or a need for unusually low endosomal pH. In contrast, since we observed that EBOV entry occurs upon arrival in Niemann-Pick C1 (NPC1)-positive endolysosomes (LE/Lys), we propose that trafficking to LE/Lys is a key rate-defining step. Additional experiments revealed, unexpectedly, that severe acute respiratory syndrome (SARS) S-mediated entry also begins only after a 30-min lag. Furthermore, although SARS does not require NPC1 for entry, SARS entry also begins after colocalization with NPC1. Since the only endosomal requirement for SARS entry is cathepsin L activity, we tested and provide evidence that NPC1(+) LE/Lys have higher cathepsin L activity than LE, with no detectable activity in earlier endosomes. Our findings suggest that both EBOV and SARS traffic deep into the endocytic pathway for entry and that they do so to access higher cathepsin activity.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Virginie</li></region></list><tree><country name="États-Unis"><region name="Virginie"><name sortKey="Mingo, Rebecca M" sort="Mingo, Rebecca M" uniqKey="Mingo R" first="Rebecca M" last="Mingo">Rebecca M. Mingo</name></region><name sortKey="Casanova, James E" sort="Casanova, James E" uniqKey="Casanova J" first="James E" last="Casanova">James E. Casanova</name><name sortKey="D Souza, Ryan S" sort="D Souza, Ryan S" uniqKey="D Souza R" first="Ryan S" last="D'Souza">Ryan S. D'Souza</name><name sortKey="Nelson, Elizabeth A" sort="Nelson, Elizabeth A" uniqKey="Nelson E" first="Elizabeth A" last="Nelson">Elizabeth A. Nelson</name><name sortKey="Schornberg, Kathryn L" sort="Schornberg, Kathryn L" uniqKey="Schornberg K" first="Kathryn L" last="Schornberg">Kathryn L. Schornberg</name><name sortKey="Shoemaker, Charles J" sort="Shoemaker, Charles J" uniqKey="Shoemaker C" first="Charles J" last="Shoemaker">Charles J. Shoemaker</name><name sortKey="Simmons, James A" sort="Simmons, James A" uniqKey="Simmons J" first="James A" last="Simmons">James A. Simmons</name><name sortKey="White, Judith M" sort="White, Judith M" uniqKey="White J" first="Judith M" last="White">Judith M. White</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001323 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001323 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:25552710
|texte= Ebola virus and severe acute respiratory syndrome coronavirus display late cell entry kinetics: evidence that transport to NPC1+ endolysosomes is a rate-defining step.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:25552710" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |