Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays.
Identifieur interne : 001047 ( Main/Exploration ); précédent : 001046; suivant : 001048Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays.
Auteurs : Samadhan J. Jadhao [États-Unis] ; Larry J. Anderson [États-Unis]Source :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- immunologie : Antigènes viraux, Lignée cellulaire tumorale, Protéines virales, Virus respiratoire syncytial humain.
- sang : Anticorps antiviraux.
- virologie : Lignée cellulaire tumorale.
- Humains, Techniques immunoenzymatiques.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Antibodies, Viral.
- chemical , immunology : Antigens, Viral, Viral Proteins.
- immunology : Cell Line, Tumor, Respiratory Syncytial Virus, Human.
- virology : Cell Line, Tumor.
- Humans, Immunoenzyme Techniques.
Abstract
Enzyme immunoassays (EIAs) to detect and quantify antibodies against respiratory syncytial virus (RSV) and RSV proteins in human plasma or sera are described. The first EIA uses RSV lysate antigens produced in HEp-2 cell line. The second EIA uses RSV F or G gene-expressed antigen in HEp-2 cells. The third EIA uses 30-amino acid synthetic peptides from central conserved region of G protein of RSV A2 or RSV B1 virus and a peptide from the SARS CoV nucleoprotein as a negative control peptide. All three EIAs have been evaluated for detecting and quantifying the respective antibodies in human sera or plasma.
DOI: 10.1007/978-1-4939-3687-8_4
PubMed: 27464686
Affiliations:
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Le document en format XML
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Anderson</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pediatrics, School of Medicine, Emory University and Children's Healthcare of Atlanta, Atlanta, GA, 30322, USA. larry.anderson@emory.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pediatrics, School of Medicine, Emory University and Children's Healthcare of Atlanta, Atlanta, GA, 30322</wicri:regionArea><wicri:noRegion>30322</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27464686</idno><idno type="pmid">27464686</idno><idno type="doi">10.1007/978-1-4939-3687-8_4</idno><idno type="wicri:Area/PubMed/Corpus">000C27</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000C27</idno><idno type="wicri:Area/PubMed/Curation">000C27</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000C27</idno><idno type="wicri:Area/PubMed/Checkpoint">000C74</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000C74</idno><idno type="wicri:Area/Ncbi/Merge">002C86</idno><idno type="wicri:Area/Ncbi/Curation">002C86</idno><idno type="wicri:Area/Ncbi/Checkpoint">002C86</idno><idno type="wicri:Area/Main/Merge">001049</idno><idno type="wicri:Area/Main/Curation">001047</idno><idno type="wicri:Area/Main/Exploration">001047</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays.</title><author><name sortKey="Jadhao, Samadhan J" sort="Jadhao, Samadhan J" uniqKey="Jadhao S" first="Samadhan J" last="Jadhao">Samadhan J. Jadhao</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pediatrics, School of Medicine, Emory University and Children's Healthcare of Atlanta, Atlanta, GA, 30322, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pediatrics, School of Medicine, Emory University and Children's Healthcare of Atlanta, Atlanta, GA, 30322</wicri:regionArea><wicri:noRegion>30322</wicri:noRegion></affiliation></author><author><name sortKey="Anderson, Larry J" sort="Anderson, Larry J" uniqKey="Anderson L" first="Larry J" last="Anderson">Larry J. Anderson</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pediatrics, School of Medicine, Emory University and Children's Healthcare of Atlanta, Atlanta, GA, 30322, USA. larry.anderson@emory.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pediatrics, School of Medicine, Emory University and Children's Healthcare of Atlanta, Atlanta, GA, 30322</wicri:regionArea><wicri:noRegion>30322</wicri:noRegion></affiliation></author></analytic><series><title level="j">Methods in molecular biology (Clifton, N.J.)</title><idno type="eISSN">1940-6029</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (blood)</term><term>Antigens, Viral (immunology)</term><term>Cell Line, Tumor (immunology)</term><term>Cell Line, Tumor (virology)</term><term>Humans</term><term>Immunoenzyme Techniques</term><term>Respiratory Syncytial Virus, Human (immunology)</term><term>Viral Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps antiviraux (sang)</term><term>Antigènes viraux (immunologie)</term><term>Humains</term><term>Lignée cellulaire tumorale (immunologie)</term><term>Lignée cellulaire tumorale (virologie)</term><term>Protéines virales (immunologie)</term><term>Techniques immunoenzymatiques</term><term>Virus respiratoire syncytial humain (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Viral</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes viraux</term><term>Lignée cellulaire tumorale</term><term>Protéines virales</term><term>Virus respiratoire syncytial humain</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Cell Line, Tumor</term><term>Respiratory Syncytial Virus, Human</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Lignée cellulaire tumorale</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Cell Line, Tumor</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Immunoenzyme Techniques</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term><term>Techniques immunoenzymatiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Enzyme immunoassays (EIAs) to detect and quantify antibodies against respiratory syncytial virus (RSV) and RSV proteins in human plasma or sera are described. The first EIA uses RSV lysate antigens produced in HEp-2 cell line. The second EIA uses RSV F or G gene-expressed antigen in HEp-2 cells. The third EIA uses 30-amino acid synthetic peptides from central conserved region of G protein of RSV A2 or RSV B1 virus and a peptide from the SARS CoV nucleoprotein as a negative control peptide. All three EIAs have been evaluated for detecting and quantifying the respective antibodies in human sera or plasma.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Jadhao, Samadhan J" sort="Jadhao, Samadhan J" uniqKey="Jadhao S" first="Samadhan J" last="Jadhao">Samadhan J. Jadhao</name></noRegion><name sortKey="Anderson, Larry J" sort="Anderson, Larry J" uniqKey="Anderson L" first="Larry J" last="Anderson">Larry J. Anderson</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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