Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays.
Identifieur interne : 000C27 ( PubMed/Curation ); précédent : 000C26; suivant : 000C28Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays.
Auteurs : Samadhan J. Jadhao [États-Unis] ; Larry J. Anderson [États-Unis]Source :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- immunologie : Antigènes viraux, Lignée cellulaire tumorale, Protéines virales, Virus respiratoire syncytial humain.
- sang : Anticorps antiviraux.
- virologie : Lignée cellulaire tumorale.
- Humains, Techniques immunoenzymatiques.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Antibodies, Viral.
- chemical , immunology : Antigens, Viral, Viral Proteins.
- immunology : Cell Line, Tumor, Respiratory Syncytial Virus, Human.
- virology : Cell Line, Tumor.
- Humans, Immunoenzyme Techniques.
Abstract
Enzyme immunoassays (EIAs) to detect and quantify antibodies against respiratory syncytial virus (RSV) and RSV proteins in human plasma or sera are described. The first EIA uses RSV lysate antigens produced in HEp-2 cell line. The second EIA uses RSV F or G gene-expressed antigen in HEp-2 cells. The third EIA uses 30-amino acid synthetic peptides from central conserved region of G protein of RSV A2 or RSV B1 virus and a peptide from the SARS CoV nucleoprotein as a negative control peptide. All three EIAs have been evaluated for detecting and quantifying the respective antibodies in human sera or plasma.
DOI: 10.1007/978-1-4939-3687-8_4
PubMed: 27464686
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pubmed:27464686Le document en format XML
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<term>Antigènes viraux (immunologie)</term>
<term>Humains</term>
<term>Lignée cellulaire tumorale (immunologie)</term>
<term>Lignée cellulaire tumorale (virologie)</term>
<term>Protéines virales (immunologie)</term>
<term>Techniques immunoenzymatiques</term>
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<front><div type="abstract" xml:lang="en">Enzyme immunoassays (EIAs) to detect and quantify antibodies against respiratory syncytial virus (RSV) and RSV proteins in human plasma or sera are described. The first EIA uses RSV lysate antigens produced in HEp-2 cell line. The second EIA uses RSV F or G gene-expressed antigen in HEp-2 cells. The third EIA uses 30-amino acid synthetic peptides from central conserved region of G protein of RSV A2 or RSV B1 virus and a peptide from the SARS CoV nucleoprotein as a negative control peptide. All three EIAs have been evaluated for detecting and quantifying the respective antibodies in human sera or plasma.</div>
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