Mapping of antigenic sites on the nucleocapsid protein of the severe acute respiratory syndrome coronavirus
Identifieur interne : 005B10 ( Main/Curation ); précédent : 005B09; suivant : 005B11Mapping of antigenic sites on the nucleocapsid protein of the severe acute respiratory syndrome coronavirus
Auteurs : YUXIAN HE [États-Unis] ; YUSEN ZHOU [République populaire de Chine] ; HAO WU [République populaire de Chine] ; ZHIHUA KOU [République populaire de Chine] ; SHUWEN LIU [États-Unis] ; SHIBO JIANG [États-Unis]Source :
- Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2004.
Descripteurs français
- Pascal (Inist)
English descriptors
Abstract
Antigenic sites on the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) were mapped by Pepscan analysis with overlapping peptides that span the N protein sequence. Two major immunodominant epitopes located in the C-terminal region (amino acids [aal 362 to 412) and middle region (aa 153 to 178) reacted with more than 75% of sera from SARS patients. Several minor immunodominant epitopes were reactive with about 50% of the SARS sera. Antisera from mice immunized with inactivated SARS-CoV recognized the two major immunodominant epitopes and one antigenic site located adjacent to the N-terminal region (aa 76 to 101), which did not react with the sera from SARS patients. Several monoclonal antibodies against SARS-CoV bound to the N- or C-terminal antigenic sites. These results suggest that the above antigenic sites on the N protein are important in eliciting humoral immune response against SARS-CoV in humans and animals and can be used as antigens for developing diagnostic tests.
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<front><div type="abstract" xml:lang="en">Antigenic sites on the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) were mapped by Pepscan analysis with overlapping peptides that span the N protein sequence. Two major immunodominant epitopes located in the C-terminal region (amino acids [aal 362 to 412) and middle region (aa 153 to 178) reacted with more than 75% of sera from SARS patients. Several minor immunodominant epitopes were reactive with about 50% of the SARS sera. Antisera from mice immunized with inactivated SARS-CoV recognized the two major immunodominant epitopes and one antigenic site located adjacent to the N-terminal region (aa 76 to 101), which did not react with the sera from SARS patients. Several monoclonal antibodies against SARS-CoV bound to the N- or C-terminal antigenic sites. These results suggest that the above antigenic sites on the N protein are important in eliciting humoral immune response against SARS-CoV in humans and animals and can be used as antigens for developing diagnostic tests.</div>
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