A double-inactivated whole virus candidate SARS coronavirus vaccine stimulates neutralising and protective antibody responses
Identifieur interne : 004952 ( Main/Curation ); précédent : 004951; suivant : 004953A double-inactivated whole virus candidate SARS coronavirus vaccine stimulates neutralising and protective antibody responses
Auteurs : Martin Spruth ; Otfried Kistner ; Helga Savidis-Dacho ; Elisabeth Hitter ; Brian Crowe ; Marijan Gerencer ; Peter Brühl ; Leopold Grillberger ; Manfred Reiter ; Christa Tauer ; Wolfgang Mundt ; P. Noel BarrettSource :
- Vaccine [ 0264-410X ] ; 2005.
Descripteurs français
- KwdFr :
- Adjuvants immunologiques (pharmacologie), Animaux, Anticorps antiviraux (analyse), Anticorps antiviraux (biosynthèse), Cellules Vero, Femelle, Fermentation, Immunisation, Relation dose-réponse (immunologie), Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (immunologie), Technique de Western, Techniques de culture de tissus, Test ELISA, Tests de neutralisation, Vaccins antiviraux (immunologie), Vaccins inactivés (immunologie), Virus du SRAS (immunologie), Électrophorèse sur gel de polyacrylamide.
- MESH :
- analyse : Anticorps antiviraux.
- biosynthèse : Anticorps antiviraux.
- immunologie : Syndrome respiratoire aigu sévère, Vaccins antiviraux, Vaccins inactivés, Virus du SRAS.
- pharmacologie : Adjuvants immunologiques.
- Animaux, Cellules Vero, Femelle, Fermentation, Immunisation, Relation dose-réponse (immunologie), Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère, Technique de Western, Techniques de culture de tissus, Test ELISA, Tests de neutralisation, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Adjuvants, Immunologic (pharmacology), Animals, Antibodies, Viral (analysis), Antibodies, Viral (biosynthesis), Blotting, Western, Chlorocebus aethiops, Dose-Response Relationship, Immunologic, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Female, Fermentation, Immunization, Mice, Mice, Inbred BALB C, Neutralization Tests, SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (prevention & control), Tissue Culture Techniques, Vaccines, Inactivated (immunology), Vero Cells, Viral Vaccines (immunology).
- MESH :
- chemical , analysis : Antibodies, Viral.
- chemical , biosynthesis : Antibodies, Viral.
- chemical , immunology : Vaccines, Inactivated, Viral Vaccines.
- chemical , pharmacology : Adjuvants, Immunologic.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- prevention & control : Severe Acute Respiratory Syndrome.
- Animals, Blotting, Western, Chlorocebus aethiops, Dose-Response Relationship, Immunologic, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Female, Fermentation, Immunization, Mice, Mice, Inbred BALB C, Neutralization Tests, Tissue Culture Techniques, Vero Cells.
Abstract
A double-inactivated, candidate whole virus vaccine against severe acute respiratory syndrome associated coronavirus (SARS-CoV) was developed and manufactured at large scale using fermenter cultures of serum protein free Vero cells. A two step inactivation procedure involving sequential formaldehyde and U.V. inactivation was utilised in order to ensure an extremely high safety margin with respect to residual infectivity. The immunogenicity of this double-inactivated vaccine was characterised in the mouse model. Mice that were immunised twice with the candidate SARS-CoV vaccine developed high antibody titres against the SARS-CoV spike protein and high levels of neutralising antibodies. The use of the adjuvant Al(OH)3 had only a minor effect on the immunogenicity of the vaccine. In addition, cell mediated immunity as measured by interferon-γ and interleukin-4 stimulation, was elicited by vaccination. Moreover, the vaccine confers protective immunity as demonstrated by prevention of SARS-CoV replication in the respiratory tract of mice after intranasal challenge with SARS-CoV. Protection of mice was correlated to antibody titre against the SARS-CoV S protein and neutralising antibody titre.
Url:
DOI: 10.1016/j.vaccine.2005.08.055
PubMed: 16214268
PubMed Central: 7115667
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PMC:7115667Le document en format XML
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Noel" last="Barrett">P. Noel Barrett</name></author></analytic><series><title level="j">Vaccine</title><idno type="ISSN">0264-410X</idno><idno type="eISSN">1873-2518</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adjuvants, Immunologic (pharmacology)</term><term>Animals</term><term>Antibodies, Viral (analysis)</term><term>Antibodies, Viral (biosynthesis)</term><term>Blotting, Western</term><term>Chlorocebus aethiops</term><term>Dose-Response Relationship, Immunologic</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Female</term><term>Fermentation</term><term>Immunization</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Neutralization Tests</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (prevention & control)</term><term>Tissue Culture Techniques</term><term>Vaccines, Inactivated (immunology)</term><term>Vero Cells</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adjuvants immunologiques (pharmacologie)</term><term>Animaux</term><term>Anticorps antiviraux (analyse)</term><term>Anticorps antiviraux (biosynthèse)</term><term>Cellules Vero</term><term>Femelle</term><term>Fermentation</term><term>Immunisation</term><term>Relation dose-réponse (immunologie)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Syndrome respiratoire aigu sévère ()</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Technique de Western</term><term>Techniques de culture de tissus</term><term>Test ELISA</term><term>Tests de neutralisation</term><term>Vaccins antiviraux (immunologie)</term><term>Vaccins inactivés (immunologie)</term><term>Virus du SRAS (immunologie)</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Vaccines, Inactivated</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Adjuvants, Immunologic</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term><term>Vaccins antiviraux</term><term>Vaccins inactivés</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Adjuvants immunologiques</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Chlorocebus aethiops</term><term>Dose-Response Relationship, Immunologic</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Female</term><term>Fermentation</term><term>Immunization</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Neutralization Tests</term><term>Tissue Culture Techniques</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Femelle</term><term>Fermentation</term><term>Immunisation</term><term>Relation dose-réponse (immunologie)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Syndrome respiratoire aigu sévère</term><term>Technique de Western</term><term>Techniques de culture de tissus</term><term>Test ELISA</term><term>Tests de neutralisation</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>A double-inactivated, candidate whole virus vaccine against severe acute respiratory syndrome associated coronavirus (SARS-CoV) was developed and manufactured at large scale using fermenter cultures of serum protein free Vero cells. A two step inactivation procedure involving sequential formaldehyde and U.V. inactivation was utilised in order to ensure an extremely high safety margin with respect to residual infectivity. The immunogenicity of this double-inactivated vaccine was characterised in the mouse model. Mice that were immunised twice with the candidate SARS-CoV vaccine developed high antibody titres against the SARS-CoV spike protein and high levels of neutralising antibodies. The use of the adjuvant Al(OH)<sub>3</sub> had only a minor effect on the immunogenicity of the vaccine. In addition, cell mediated immunity as measured by interferon-γ and interleukin-4 stimulation, was elicited by vaccination. Moreover, the vaccine confers protective immunity as demonstrated by prevention of SARS-CoV replication in the respiratory tract of mice after intranasal challenge with SARS-CoV. Protection of mice was correlated to antibody titre against the SARS-CoV S protein and neutralising antibody titre.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Peiris, J S M" uniqKey="Peiris J">J.S.M. Peiris</name></author><author><name sortKey="Lai, S T" uniqKey="Lai S">S.T. Lai</name></author><author><name sortKey="Poon, L L M" uniqKey="Poon L">L.L.M. Poon</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y. Guan</name></author><author><name sortKey="Yam, L Y C" uniqKey="Yam L">L.Y.C. Yam</name></author><author><name sortKey="Lim, W" uniqKey="Lim W">W. Lim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author><author><name sortKey="Gunther, S" uniqKey="Gunther S">S. Gunther</name></author><author><name sortKey="Preiser, W" uniqKey="Preiser W">W. 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