Direct template matching reveals a host subcellular membrane gyroid cubic structure that is associated with SARS virus.
Identifieur interne : 004862 ( Main/Curation ); précédent : 004861; suivant : 004863Direct template matching reveals a host subcellular membrane gyroid cubic structure that is associated with SARS virus.
Auteurs : Zakaria A. Almsherqi [Singapour] ; Craig S. Mclachlan ; Peter Mossop ; Kèvin Knoops ; Yuru DengSource :
- Redox report : communications in free radical research [ 1743-2928 ] ; 2005.
Descripteurs français
- KwdFr :
- Assemblage viral, Conformation moléculaire, Espèces réactives de l'oxygène, Logiciel, Membrane cellulaire (métabolisme), Microscopie électronique à transmission, Modèles théoriques, Oxydoréduction, Radicaux libres, Traitement d'image par ordinateur, Virion (ultrastructure), Virus du SRAS (métabolisme), Virus du SRAS (ultrastructure).
- MESH :
English descriptors
- KwdEn :
- Cell Membrane (metabolism), Free Radicals, Image Processing, Computer-Assisted, Microscopy, Electron, Transmission, Models, Theoretical, Molecular Conformation, Oxidation-Reduction, Reactive Oxygen Species, SARS Virus (metabolism), SARS Virus (ultrastructure), Software, Virion (ultrastructure), Virus Assembly.
- MESH :
- chemical : Free Radicals, Reactive Oxygen Species.
- metabolism : Cell Membrane, SARS Virus.
- ultrastructure : SARS Virus, Virion.
- Image Processing, Computer-Assisted, Microscopy, Electron, Transmission, Models, Theoretical, Molecular Conformation, Oxidation-Reduction, Software, Virus Assembly.
Abstract
Viral infection can result in alterations to the host subcellular membrane. This is often reported when using transmission electron microscopy (TEM), resulting in a description of tubuloreticular membrane subcellular ultrastructure rather than a definition based on 3-D morphology. 2-D TEM micrographs depicting subcellular membrane changes are associated with subcellular SARS virion particles [Goldsmith CS, Tatti KM, Ksiazek TG et al. Ultra-structural characterization of SARS coronavirus. Emerg Infect Dis 2004; 10: 320-326]. In the present study, we have defined the 2-D membrane pattern and shape associated with the SARS virus infection. This is by using a direct template matching method to determine what the 3-D structure of the SARS virus associated host membrane change would be. The TEM image for our purposes is defined on 2-D information, such as the membrane having undergone proliferation and from pattern recognition suggesting that the membrane-described pattern is possibly a gyroid type of membrane. Features of the membrane were used to compute and match the gyroid structure with an existing 2-D TEM micrograph, where it was revealed that the membrane structure was indeed a gyroid-based cubic membrane. The 2-D gyroid computer-simulated image that was used to match the electron micrograph of interest was derived from a mathematically well-defined 3-D structure, and it is from this 3-D derivative that allows us to make inferences about the 3-D structure of this membrane. In conclusion, we demonstrate that a 3-D structure can be defined from a 2-D membrane patterned image and that a SARS viral associated membrane change has been identified as cubic membrane morphology. Possible mechanisms for this cubic membrane change are discussed with respect to viral severity, persistence and free radical production.
DOI: 10.1179/135100005X57373
PubMed: 16156956
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pubmed:16156956Le document en format XML
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<term>Molecular Conformation</term>
<term>Oxidation-Reduction</term>
<term>Reactive Oxygen Species</term>
<term>SARS Virus (metabolism)</term>
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<term>Microscopie électronique à transmission</term>
<term>Modèles théoriques</term>
<term>Oxydoréduction</term>
<term>Radicaux libres</term>
<term>Traitement d'image par ordinateur</term>
<term>Virion (ultrastructure)</term>
<term>Virus du SRAS (métabolisme)</term>
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<term>Espèces réactives de l'oxygène</term>
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<term>Microscopie électronique à transmission</term>
<term>Modèles théoriques</term>
<term>Oxydoréduction</term>
<term>Radicaux libres</term>
<term>Traitement d'image par ordinateur</term>
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<front><div type="abstract" xml:lang="en">Viral infection can result in alterations to the host subcellular membrane. This is often reported when using transmission electron microscopy (TEM), resulting in a description of tubuloreticular membrane subcellular ultrastructure rather than a definition based on 3-D morphology. 2-D TEM micrographs depicting subcellular membrane changes are associated with subcellular SARS virion particles [Goldsmith CS, Tatti KM, Ksiazek TG et al. Ultra-structural characterization of SARS coronavirus. Emerg Infect Dis 2004; 10: 320-326]. In the present study, we have defined the 2-D membrane pattern and shape associated with the SARS virus infection. This is by using a direct template matching method to determine what the 3-D structure of the SARS virus associated host membrane change would be. The TEM image for our purposes is defined on 2-D information, such as the membrane having undergone proliferation and from pattern recognition suggesting that the membrane-described pattern is possibly a gyroid type of membrane. Features of the membrane were used to compute and match the gyroid structure with an existing 2-D TEM micrograph, where it was revealed that the membrane structure was indeed a gyroid-based cubic membrane. The 2-D gyroid computer-simulated image that was used to match the electron micrograph of interest was derived from a mathematically well-defined 3-D structure, and it is from this 3-D derivative that allows us to make inferences about the 3-D structure of this membrane. In conclusion, we demonstrate that a 3-D structure can be defined from a 2-D membrane patterned image and that a SARS viral associated membrane change has been identified as cubic membrane morphology. Possible mechanisms for this cubic membrane change are discussed with respect to viral severity, persistence and free radical production.</div>
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