Indomethacin has a potent antiviral activity against SARS coronavirus
Identifieur interne : 004439 ( Main/Curation ); précédent : 004438; suivant : 004440Indomethacin has a potent antiviral activity against SARS coronavirus
Auteurs : Carla Amici [Italie] ; Antonino Di Caro [Italie] ; Alessandra Ciucci [Italie] ; Lucia Chiappa [Italie] ; Concetta Castilletti [Italie] ; Vito Martella [Italie] ; Nicola Decaro [Italie] ; Canio Buonavoglia [Italie] ; Maria R. Capobianchi [Italie] ; M. Gabriella Santoro [Italie]Source :
- Antiviral therapy : (London) [ 1359-6535 ] ; 2006.
Descripteurs français
- KwdFr :
- ARN viral (isolement et purification), Acide acétylsalicylique (pharmacologie), Animaux, Antiviraux (pharmacologie), Antiviraux (usage thérapeutique), Chiens, Coronavirus canin (), Fèces (virologie), Humains, Indométacine (pharmacologie), Indométacine (usage thérapeutique), Infections à coronavirus (médecine vétérinaire), Infections à coronavirus (traitement médicamenteux), Interféron alpha (pharmacologie), Lignée cellulaire, Maladies des chiens (traitement médicamenteux), Relation dose-effet des médicaments, Ribavirine (pharmacologie), Réplication virale (), Virus du SRAS ().
- MESH :
- isolement et purification : ARN viral.
- médecine vétérinaire : Infections à coronavirus.
- pharmacologie : Acide acétylsalicylique, Antiviraux, Indométacine, Interféron alpha, Ribavirine.
- traitement médicamenteux : Infections à coronavirus, Maladies des chiens.
- usage thérapeutique : Antiviraux, Indométacine.
- virologie : Fèces.
- Pascal (Inist)
English descriptors
- KwdEn :
- Animals, Antiviral, Antiviral Agents (pharmacology), Antiviral Agents (therapeutic use), Aspirin (pharmacology), Cell Line, Chlorocebus aethiops, Coronavirus Infections (drug therapy), Coronavirus Infections (veterinary), Coronavirus, Canine (drug effects), Dog Diseases (drug therapy), Dogs, Dose-Response Relationship, Drug, Feces (virology), Humans, Indometacin, Indomethacin (pharmacology), Indomethacin (therapeutic use), Interferon-alpha (pharmacology), Non steroidal antiinflammatory agent, RNA, Viral (isolation & purification), Ribavirin (pharmacology), SARS Virus (drug effects), Severe acute respiratory syndrome virus, Tocolytic, Virus Replication (drug effects).
- MESH :
- chemical , isolation & purification : RNA, Viral.
- chemical , pharmacology : Antiviral Agents, Aspirin, Indomethacin, Interferon-alpha, Ribavirin.
- chemical , therapeutic use : Antiviral Agents, Indomethacin.
- drug effects : Coronavirus, Canine, SARS Virus, Virus Replication.
- drug therapy : Coronavirus Infections, Dog Diseases.
- veterinary : Coronavirus Infections.
- virology : Feces.
- Animals, Cell Line, Chlorocebus aethiops, Dogs, Dose-Response Relationship, Drug, Humans.
Abstract
Severe acute respiratory syndrome (SARS) is a newly emerging, highly transmissible and fatal disease caused by a previously unknown coronavirus (SARS-CoV). Existing in non-identified animal reservoirs, SARS-CoV continues to represent a threat to humans because there is no effective specific antiviral therapy for coronavirus infections. Objectives: Starting from the observation that cyclopentenone cyclooxygenase (COX) metabolites are active against several RNA viruses, we investigated the effect of the COX inhibitor indomethacin on coronavirus replication. Methods: Work involving infectious SARS-CoV was performed in biosafety level 3 facilities. SARS-CoV was grown in monkey VERO cells and human lung epithelial A549 cells, while canine coronavirus (CCoV) was grown in A72 canine cells. Antiviral activity was analysed by determining infective virus titres by TCID50, viral RNA synthesis by Northern blot analysis and real-time RT-PCR, and viral protein synthesis by SDS-PAGE analysis after 35S-methionine-labelling. Antiviral efficacy in vivo was determined by evaluating virus titres in CCoV-infected dogs treated orally with 1 mg/kg body weight indomethacin (INDO). Results: Unexpectedly, we found that INDO has a potent direct antiviral activity against the coronaviruses SARS-CoV and CCoV. INDO does not affect coronavirus binding or entry into host cells, but acts by blocking viral RNA synthesis at cytoprotective doses. This effect is independent of cyclooxygenase inhibition. INDO's potent antiviral activity (>1,000-fold reduction in virus yield) was confirmed in vivo in CCoV-infected dogs. Conclusions: The results identify INDO as a potent inhibitor of coronavirus replication and suggest that, having both anti-inflammatory and antiviral activity, INDO could be beneficial in SARS therapy.
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000421
- to stream PascalFrancis, to step Curation: Pour aller vers cette notice dans l'étape Curation :000568
- to stream PascalFrancis, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000461
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :004662
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001E95
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :001E95
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :002144
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :001873
- to stream Ncbi, to step Curation: Pour aller vers cette notice dans l'étape Curation :001873
- to stream Ncbi, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001873
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :004036
Links to Exploration step
Pascal:07-0058292Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Indomethacin has a potent antiviral activity against SARS coronavirus</title>
<author><name sortKey="Amici, Carla" sort="Amici, Carla" uniqKey="Amici C" first="Carla" last="Amici">Carla Amici</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Biology, University of Rome Tor Vergata</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Di Caro, Antonino" sort="Di Caro, Antonino" uniqKey="Di Caro A" first="Antonino" last="Di Caro">Antonino Di Caro</name>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Laboratory of Virology, National Institute for Infectious Diseases 'L. Spallanzani'</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ciucci, Alessandra" sort="Ciucci, Alessandra" uniqKey="Ciucci A" first="Alessandra" last="Ciucci">Alessandra Ciucci</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Biology, University of Rome Tor Vergata</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chiappa, Lucia" sort="Chiappa, Lucia" uniqKey="Chiappa L" first="Lucia" last="Chiappa">Lucia Chiappa</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Biology, University of Rome Tor Vergata</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Castilletti, Concetta" sort="Castilletti, Concetta" uniqKey="Castilletti C" first="Concetta" last="Castilletti">Concetta Castilletti</name>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Laboratory of Virology, National Institute for Infectious Diseases 'L. Spallanzani'</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Martella, Vito" sort="Martella, Vito" uniqKey="Martella V" first="Vito" last="Martella">Vito Martella</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Faculty of Veterinary Medicine, University of Bari</s1>
<s2>Bari</s2>
<s3>ITA</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<wicri:noRegion>Bari</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Decaro, Nicola" sort="Decaro, Nicola" uniqKey="Decaro N" first="Nicola" last="Decaro">Nicola Decaro</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Faculty of Veterinary Medicine, University of Bari</s1>
<s2>Bari</s2>
<s3>ITA</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<wicri:noRegion>Bari</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Buonavoglia, Canio" sort="Buonavoglia, Canio" uniqKey="Buonavoglia C" first="Canio" last="Buonavoglia">Canio Buonavoglia</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Faculty of Veterinary Medicine, University of Bari</s1>
<s2>Bari</s2>
<s3>ITA</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<wicri:noRegion>Bari</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Capobianchi, Maria R" sort="Capobianchi, Maria R" uniqKey="Capobianchi M" first="Maria R." last="Capobianchi">Maria R. Capobianchi</name>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Laboratory of Virology, National Institute for Infectious Diseases 'L. Spallanzani'</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Santoro, M Gabriella" sort="Santoro, M Gabriella" uniqKey="Santoro M" first="M. Gabriella" last="Santoro">M. Gabriella Santoro</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Biology, University of Rome Tor Vergata</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">07-0058292</idno>
<date when="2006">2006</date>
<idno type="stanalyst">PASCAL 07-0058292 INIST</idno>
<idno type="RBID">Pascal:07-0058292</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000421</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000568</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000461</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000461</idno>
<idno type="wicri:doubleKey">1359-6535:2006:Amici C:indomethacin:has:a</idno>
<idno type="wicri:Area/Main/Merge">004662</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:17302372</idno>
<idno type="wicri:Area/PubMed/Corpus">001E95</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001E95</idno>
<idno type="wicri:Area/PubMed/Curation">001E95</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001E95</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002144</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002144</idno>
<idno type="wicri:Area/Ncbi/Merge">001873</idno>
<idno type="wicri:Area/Ncbi/Curation">001873</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">001873</idno>
<idno type="wicri:doubleKey">1359-6535:2006:Amici C:indomethacin:has:a</idno>
<idno type="wicri:Area/Main/Merge">004036</idno>
<idno type="wicri:Area/Main/Curation">004439</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Indomethacin has a potent antiviral activity against SARS coronavirus</title>
<author><name sortKey="Amici, Carla" sort="Amici, Carla" uniqKey="Amici C" first="Carla" last="Amici">Carla Amici</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Biology, University of Rome Tor Vergata</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Di Caro, Antonino" sort="Di Caro, Antonino" uniqKey="Di Caro A" first="Antonino" last="Di Caro">Antonino Di Caro</name>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Laboratory of Virology, National Institute for Infectious Diseases 'L. Spallanzani'</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ciucci, Alessandra" sort="Ciucci, Alessandra" uniqKey="Ciucci A" first="Alessandra" last="Ciucci">Alessandra Ciucci</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Biology, University of Rome Tor Vergata</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chiappa, Lucia" sort="Chiappa, Lucia" uniqKey="Chiappa L" first="Lucia" last="Chiappa">Lucia Chiappa</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Biology, University of Rome Tor Vergata</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Castilletti, Concetta" sort="Castilletti, Concetta" uniqKey="Castilletti C" first="Concetta" last="Castilletti">Concetta Castilletti</name>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Laboratory of Virology, National Institute for Infectious Diseases 'L. Spallanzani'</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Martella, Vito" sort="Martella, Vito" uniqKey="Martella V" first="Vito" last="Martella">Vito Martella</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Faculty of Veterinary Medicine, University of Bari</s1>
<s2>Bari</s2>
<s3>ITA</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<wicri:noRegion>Bari</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Decaro, Nicola" sort="Decaro, Nicola" uniqKey="Decaro N" first="Nicola" last="Decaro">Nicola Decaro</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Faculty of Veterinary Medicine, University of Bari</s1>
<s2>Bari</s2>
<s3>ITA</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<wicri:noRegion>Bari</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Buonavoglia, Canio" sort="Buonavoglia, Canio" uniqKey="Buonavoglia C" first="Canio" last="Buonavoglia">Canio Buonavoglia</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Faculty of Veterinary Medicine, University of Bari</s1>
<s2>Bari</s2>
<s3>ITA</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<wicri:noRegion>Bari</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Capobianchi, Maria R" sort="Capobianchi, Maria R" uniqKey="Capobianchi M" first="Maria R." last="Capobianchi">Maria R. Capobianchi</name>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Laboratory of Virology, National Institute for Infectious Diseases 'L. Spallanzani'</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Santoro, M Gabriella" sort="Santoro, M Gabriella" uniqKey="Santoro M" first="M. Gabriella" last="Santoro">M. Gabriella Santoro</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Biology, University of Rome Tor Vergata</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Antiviral therapy : (London)</title>
<title level="j" type="abbreviated">Antivir. ther. : (Lond.)</title>
<idno type="ISSN">1359-6535</idno>
<imprint><date when="2006">2006</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Antiviral therapy : (London)</title>
<title level="j" type="abbreviated">Antivir. ther. : (Lond.)</title>
<idno type="ISSN">1359-6535</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antiviral</term>
<term>Antiviral Agents (pharmacology)</term>
<term>Antiviral Agents (therapeutic use)</term>
<term>Aspirin (pharmacology)</term>
<term>Cell Line</term>
<term>Chlorocebus aethiops</term>
<term>Coronavirus Infections (drug therapy)</term>
<term>Coronavirus Infections (veterinary)</term>
<term>Coronavirus, Canine (drug effects)</term>
<term>Dog Diseases (drug therapy)</term>
<term>Dogs</term>
<term>Dose-Response Relationship, Drug</term>
<term>Feces (virology)</term>
<term>Humans</term>
<term>Indometacin</term>
<term>Indomethacin (pharmacology)</term>
<term>Indomethacin (therapeutic use)</term>
<term>Interferon-alpha (pharmacology)</term>
<term>Non steroidal antiinflammatory agent</term>
<term>RNA, Viral (isolation & purification)</term>
<term>Ribavirin (pharmacology)</term>
<term>SARS Virus (drug effects)</term>
<term>Severe acute respiratory syndrome virus</term>
<term>Tocolytic</term>
<term>Virus Replication (drug effects)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (isolement et purification)</term>
<term>Acide acétylsalicylique (pharmacologie)</term>
<term>Animaux</term>
<term>Antiviraux (pharmacologie)</term>
<term>Antiviraux (usage thérapeutique)</term>
<term>Chiens</term>
<term>Coronavirus canin ()</term>
<term>Fèces (virologie)</term>
<term>Humains</term>
<term>Indométacine (pharmacologie)</term>
<term>Indométacine (usage thérapeutique)</term>
<term>Infections à coronavirus (médecine vétérinaire)</term>
<term>Infections à coronavirus (traitement médicamenteux)</term>
<term>Interféron alpha (pharmacologie)</term>
<term>Lignée cellulaire</term>
<term>Maladies des chiens (traitement médicamenteux)</term>
<term>Relation dose-effet des médicaments</term>
<term>Ribavirine (pharmacologie)</term>
<term>Réplication virale ()</term>
<term>Virus du SRAS ()</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antiviral Agents</term>
<term>Aspirin</term>
<term>Indomethacin</term>
<term>Interferon-alpha</term>
<term>Ribavirin</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="therapeutic use" xml:lang="en"><term>Antiviral Agents</term>
<term>Indomethacin</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Coronavirus, Canine</term>
<term>SARS Virus</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="MESH" qualifier="drug therapy" xml:lang="en"><term>Coronavirus Infections</term>
<term>Dog Diseases</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ARN viral</term>
</keywords>
<keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Acide acétylsalicylique</term>
<term>Antiviraux</term>
<term>Indométacine</term>
<term>Interféron alpha</term>
<term>Ribavirine</term>
</keywords>
<keywords scheme="MESH" qualifier="traitement médicamenteux" xml:lang="fr"><term>Infections à coronavirus</term>
<term>Maladies des chiens</term>
</keywords>
<keywords scheme="MESH" qualifier="usage thérapeutique" xml:lang="fr"><term>Antiviraux</term>
<term>Indométacine</term>
</keywords>
<keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Coronavirus Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Fèces</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Feces</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cell Line</term>
<term>Chlorocebus aethiops</term>
<term>Dogs</term>
<term>Dose-Response Relationship, Drug</term>
<term>Humans</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Animaux</term>
<term>Chiens</term>
<term>Coronavirus canin</term>
<term>Humains</term>
<term>Indométacine</term>
<term>Antiviral</term>
<term>Lignée cellulaire</term>
<term>Relation dose-effet des médicaments</term>
<term>Réplication virale</term>
<term>Virus du SRAS</term>
<term>Virus syndrome respiratoire aigu sévère</term>
<term>Antiinflammatoire non stéroïde</term>
<term>Tocolytique</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is a newly emerging, highly transmissible and fatal disease caused by a previously unknown coronavirus (SARS-CoV). Existing in non-identified animal reservoirs, SARS-CoV continues to represent a threat to humans because there is no effective specific antiviral therapy for coronavirus infections. Objectives: Starting from the observation that cyclopentenone cyclooxygenase (COX) metabolites are active against several RNA viruses, we investigated the effect of the COX inhibitor indomethacin on coronavirus replication. Methods: Work involving infectious SARS-CoV was performed in biosafety level 3 facilities. SARS-CoV was grown in monkey VERO cells and human lung epithelial A549 cells, while canine coronavirus (CCoV) was grown in A72 canine cells. Antiviral activity was analysed by determining infective virus titres by TCID<sub>50</sub>
, viral RNA synthesis by Northern blot analysis and real-time RT-PCR, and viral protein synthesis by SDS-PAGE analysis after <sup>35</sup>
S-methionine-labelling. Antiviral efficacy in vivo was determined by evaluating virus titres in CCoV-infected dogs treated orally with 1 mg/kg body weight indomethacin (INDO). Results: Unexpectedly, we found that INDO has a potent direct antiviral activity against the coronaviruses SARS-CoV and CCoV. INDO does not affect coronavirus binding or entry into host cells, but acts by blocking viral RNA synthesis at cytoprotective doses. This effect is independent of cyclooxygenase inhibition. INDO's potent antiviral activity (>1,000-fold reduction in virus yield) was confirmed in vivo in CCoV-infected dogs. Conclusions: The results identify INDO as a potent inhibitor of coronavirus replication and suggest that, having both anti-inflammatory and antiviral activity, INDO could be beneficial in SARS therapy.</div>
</front>
</TEI>
<double idat="1359-6535:2006:Amici C:indomethacin:has:a"><INIST><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Indomethacin has a potent antiviral activity against SARS coronavirus</title>
<author><name sortKey="Amici, Carla" sort="Amici, Carla" uniqKey="Amici C" first="Carla" last="Amici">Carla Amici</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Biology, University of Rome Tor Vergata</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Di Caro, Antonino" sort="Di Caro, Antonino" uniqKey="Di Caro A" first="Antonino" last="Di Caro">Antonino Di Caro</name>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Laboratory of Virology, National Institute for Infectious Diseases 'L. Spallanzani'</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ciucci, Alessandra" sort="Ciucci, Alessandra" uniqKey="Ciucci A" first="Alessandra" last="Ciucci">Alessandra Ciucci</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Biology, University of Rome Tor Vergata</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chiappa, Lucia" sort="Chiappa, Lucia" uniqKey="Chiappa L" first="Lucia" last="Chiappa">Lucia Chiappa</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Biology, University of Rome Tor Vergata</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Castilletti, Concetta" sort="Castilletti, Concetta" uniqKey="Castilletti C" first="Concetta" last="Castilletti">Concetta Castilletti</name>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Laboratory of Virology, National Institute for Infectious Diseases 'L. Spallanzani'</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Martella, Vito" sort="Martella, Vito" uniqKey="Martella V" first="Vito" last="Martella">Vito Martella</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Faculty of Veterinary Medicine, University of Bari</s1>
<s2>Bari</s2>
<s3>ITA</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<wicri:noRegion>Bari</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Decaro, Nicola" sort="Decaro, Nicola" uniqKey="Decaro N" first="Nicola" last="Decaro">Nicola Decaro</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Faculty of Veterinary Medicine, University of Bari</s1>
<s2>Bari</s2>
<s3>ITA</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<wicri:noRegion>Bari</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Buonavoglia, Canio" sort="Buonavoglia, Canio" uniqKey="Buonavoglia C" first="Canio" last="Buonavoglia">Canio Buonavoglia</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Faculty of Veterinary Medicine, University of Bari</s1>
<s2>Bari</s2>
<s3>ITA</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<wicri:noRegion>Bari</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Capobianchi, Maria R" sort="Capobianchi, Maria R" uniqKey="Capobianchi M" first="Maria R." last="Capobianchi">Maria R. Capobianchi</name>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Laboratory of Virology, National Institute for Infectious Diseases 'L. Spallanzani'</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Santoro, M Gabriella" sort="Santoro, M Gabriella" uniqKey="Santoro M" first="M. Gabriella" last="Santoro">M. Gabriella Santoro</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Biology, University of Rome Tor Vergata</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">07-0058292</idno>
<date when="2006">2006</date>
<idno type="stanalyst">PASCAL 07-0058292 INIST</idno>
<idno type="RBID">Pascal:07-0058292</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000421</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000568</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000461</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000461</idno>
<idno type="wicri:doubleKey">1359-6535:2006:Amici C:indomethacin:has:a</idno>
<idno type="wicri:Area/Main/Merge">004662</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Indomethacin has a potent antiviral activity against SARS coronavirus</title>
<author><name sortKey="Amici, Carla" sort="Amici, Carla" uniqKey="Amici C" first="Carla" last="Amici">Carla Amici</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Biology, University of Rome Tor Vergata</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Di Caro, Antonino" sort="Di Caro, Antonino" uniqKey="Di Caro A" first="Antonino" last="Di Caro">Antonino Di Caro</name>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Laboratory of Virology, National Institute for Infectious Diseases 'L. Spallanzani'</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ciucci, Alessandra" sort="Ciucci, Alessandra" uniqKey="Ciucci A" first="Alessandra" last="Ciucci">Alessandra Ciucci</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Biology, University of Rome Tor Vergata</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chiappa, Lucia" sort="Chiappa, Lucia" uniqKey="Chiappa L" first="Lucia" last="Chiappa">Lucia Chiappa</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Biology, University of Rome Tor Vergata</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Castilletti, Concetta" sort="Castilletti, Concetta" uniqKey="Castilletti C" first="Concetta" last="Castilletti">Concetta Castilletti</name>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Laboratory of Virology, National Institute for Infectious Diseases 'L. Spallanzani'</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Martella, Vito" sort="Martella, Vito" uniqKey="Martella V" first="Vito" last="Martella">Vito Martella</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Faculty of Veterinary Medicine, University of Bari</s1>
<s2>Bari</s2>
<s3>ITA</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<wicri:noRegion>Bari</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Decaro, Nicola" sort="Decaro, Nicola" uniqKey="Decaro N" first="Nicola" last="Decaro">Nicola Decaro</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Faculty of Veterinary Medicine, University of Bari</s1>
<s2>Bari</s2>
<s3>ITA</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<wicri:noRegion>Bari</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Buonavoglia, Canio" sort="Buonavoglia, Canio" uniqKey="Buonavoglia C" first="Canio" last="Buonavoglia">Canio Buonavoglia</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Faculty of Veterinary Medicine, University of Bari</s1>
<s2>Bari</s2>
<s3>ITA</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<wicri:noRegion>Bari</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Capobianchi, Maria R" sort="Capobianchi, Maria R" uniqKey="Capobianchi M" first="Maria R." last="Capobianchi">Maria R. Capobianchi</name>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Laboratory of Virology, National Institute for Infectious Diseases 'L. Spallanzani'</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Santoro, M Gabriella" sort="Santoro, M Gabriella" uniqKey="Santoro M" first="M. Gabriella" last="Santoro">M. Gabriella Santoro</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Biology, University of Rome Tor Vergata</s1>
<s2>Rome</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Antiviral therapy : (London)</title>
<title level="j" type="abbreviated">Antivir. ther. : (Lond.)</title>
<idno type="ISSN">1359-6535</idno>
<imprint><date when="2006">2006</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Antiviral therapy : (London)</title>
<title level="j" type="abbreviated">Antivir. ther. : (Lond.)</title>
<idno type="ISSN">1359-6535</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antiviral</term>
<term>Indometacin</term>
<term>Non steroidal antiinflammatory agent</term>
<term>Severe acute respiratory syndrome virus</term>
<term>Tocolytic</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Indométacine</term>
<term>Antiviral</term>
<term>Virus syndrome respiratoire aigu sévère</term>
<term>Antiinflammatoire non stéroïde</term>
<term>Tocolytique</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is a newly emerging, highly transmissible and fatal disease caused by a previously unknown coronavirus (SARS-CoV). Existing in non-identified animal reservoirs, SARS-CoV continues to represent a threat to humans because there is no effective specific antiviral therapy for coronavirus infections. Objectives: Starting from the observation that cyclopentenone cyclooxygenase (COX) metabolites are active against several RNA viruses, we investigated the effect of the COX inhibitor indomethacin on coronavirus replication. Methods: Work involving infectious SARS-CoV was performed in biosafety level 3 facilities. SARS-CoV was grown in monkey VERO cells and human lung epithelial A549 cells, while canine coronavirus (CCoV) was grown in A72 canine cells. Antiviral activity was analysed by determining infective virus titres by TCID<sub>50</sub>
, viral RNA synthesis by Northern blot analysis and real-time RT-PCR, and viral protein synthesis by SDS-PAGE analysis after <sup>35</sup>
S-methionine-labelling. Antiviral efficacy in vivo was determined by evaluating virus titres in CCoV-infected dogs treated orally with 1 mg/kg body weight indomethacin (INDO). Results: Unexpectedly, we found that INDO has a potent direct antiviral activity against the coronaviruses SARS-CoV and CCoV. INDO does not affect coronavirus binding or entry into host cells, but acts by blocking viral RNA synthesis at cytoprotective doses. This effect is independent of cyclooxygenase inhibition. INDO's potent antiviral activity (>1,000-fold reduction in virus yield) was confirmed in vivo in CCoV-infected dogs. Conclusions: The results identify INDO as a potent inhibitor of coronavirus replication and suggest that, having both anti-inflammatory and antiviral activity, INDO could be beneficial in SARS therapy.</div>
</front>
</TEI>
</INIST>
<PubMed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Indomethacin has a potent antiviral activity against SARS coronavirus.</title>
<author><name sortKey="Amici, Carla" sort="Amici, Carla" uniqKey="Amici C" first="Carla" last="Amici">Carla Amici</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biology, University of Rome Tor Vergata, Rome, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Department of Biology, University of Rome Tor Vergata, Rome</wicri:regionArea>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Di Caro, Antonino" sort="Di Caro, Antonino" uniqKey="Di Caro A" first="Antonino" last="Di Caro">Antonino Di Caro</name>
</author>
<author><name sortKey="Ciucci, Alessandra" sort="Ciucci, Alessandra" uniqKey="Ciucci A" first="Alessandra" last="Ciucci">Alessandra Ciucci</name>
</author>
<author><name sortKey="Chiappa, Lucia" sort="Chiappa, Lucia" uniqKey="Chiappa L" first="Lucia" last="Chiappa">Lucia Chiappa</name>
</author>
<author><name sortKey="Castilletti, Concetta" sort="Castilletti, Concetta" uniqKey="Castilletti C" first="Concetta" last="Castilletti">Concetta Castilletti</name>
</author>
<author><name sortKey="Martella, Vito" sort="Martella, Vito" uniqKey="Martella V" first="Vito" last="Martella">Vito Martella</name>
</author>
<author><name sortKey="Decaro, Nicola" sort="Decaro, Nicola" uniqKey="Decaro N" first="Nicola" last="Decaro">Nicola Decaro</name>
</author>
<author><name sortKey="Buonavoglia, Canio" sort="Buonavoglia, Canio" uniqKey="Buonavoglia C" first="Canio" last="Buonavoglia">Canio Buonavoglia</name>
</author>
<author><name sortKey="Capobianchi, Maria R" sort="Capobianchi, Maria R" uniqKey="Capobianchi M" first="Maria R" last="Capobianchi">Maria R. Capobianchi</name>
</author>
<author><name sortKey="Santoro, M Gabriella" sort="Santoro, M Gabriella" uniqKey="Santoro M" first="M Gabriella" last="Santoro">M Gabriella Santoro</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2006">2006</date>
<idno type="RBID">pubmed:17302372</idno>
<idno type="pmid">17302372</idno>
<idno type="wicri:Area/PubMed/Corpus">001E95</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001E95</idno>
<idno type="wicri:Area/PubMed/Curation">001E95</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001E95</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002144</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002144</idno>
<idno type="wicri:Area/Ncbi/Merge">001873</idno>
<idno type="wicri:Area/Ncbi/Curation">001873</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">001873</idno>
<idno type="wicri:doubleKey">1359-6535:2006:Amici C:indomethacin:has:a</idno>
<idno type="wicri:Area/Main/Merge">004036</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Indomethacin has a potent antiviral activity against SARS coronavirus.</title>
<author><name sortKey="Amici, Carla" sort="Amici, Carla" uniqKey="Amici C" first="Carla" last="Amici">Carla Amici</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biology, University of Rome Tor Vergata, Rome, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Department of Biology, University of Rome Tor Vergata, Rome</wicri:regionArea>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Di Caro, Antonino" sort="Di Caro, Antonino" uniqKey="Di Caro A" first="Antonino" last="Di Caro">Antonino Di Caro</name>
</author>
<author><name sortKey="Ciucci, Alessandra" sort="Ciucci, Alessandra" uniqKey="Ciucci A" first="Alessandra" last="Ciucci">Alessandra Ciucci</name>
</author>
<author><name sortKey="Chiappa, Lucia" sort="Chiappa, Lucia" uniqKey="Chiappa L" first="Lucia" last="Chiappa">Lucia Chiappa</name>
</author>
<author><name sortKey="Castilletti, Concetta" sort="Castilletti, Concetta" uniqKey="Castilletti C" first="Concetta" last="Castilletti">Concetta Castilletti</name>
</author>
<author><name sortKey="Martella, Vito" sort="Martella, Vito" uniqKey="Martella V" first="Vito" last="Martella">Vito Martella</name>
</author>
<author><name sortKey="Decaro, Nicola" sort="Decaro, Nicola" uniqKey="Decaro N" first="Nicola" last="Decaro">Nicola Decaro</name>
</author>
<author><name sortKey="Buonavoglia, Canio" sort="Buonavoglia, Canio" uniqKey="Buonavoglia C" first="Canio" last="Buonavoglia">Canio Buonavoglia</name>
</author>
<author><name sortKey="Capobianchi, Maria R" sort="Capobianchi, Maria R" uniqKey="Capobianchi M" first="Maria R" last="Capobianchi">Maria R. Capobianchi</name>
</author>
<author><name sortKey="Santoro, M Gabriella" sort="Santoro, M Gabriella" uniqKey="Santoro M" first="M Gabriella" last="Santoro">M Gabriella Santoro</name>
</author>
</analytic>
<series><title level="j">Antiviral therapy</title>
<idno type="ISSN">1359-6535</idno>
<imprint><date when="2006" type="published">2006</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antiviral Agents (pharmacology)</term>
<term>Antiviral Agents (therapeutic use)</term>
<term>Aspirin (pharmacology)</term>
<term>Cell Line</term>
<term>Chlorocebus aethiops</term>
<term>Coronavirus Infections (drug therapy)</term>
<term>Coronavirus Infections (veterinary)</term>
<term>Coronavirus, Canine (drug effects)</term>
<term>Dog Diseases (drug therapy)</term>
<term>Dogs</term>
<term>Dose-Response Relationship, Drug</term>
<term>Feces (virology)</term>
<term>Humans</term>
<term>Indomethacin (pharmacology)</term>
<term>Indomethacin (therapeutic use)</term>
<term>Interferon-alpha (pharmacology)</term>
<term>RNA, Viral (isolation & purification)</term>
<term>Ribavirin (pharmacology)</term>
<term>SARS Virus (drug effects)</term>
<term>Virus Replication (drug effects)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (isolement et purification)</term>
<term>Acide acétylsalicylique (pharmacologie)</term>
<term>Animaux</term>
<term>Antiviraux (pharmacologie)</term>
<term>Antiviraux (usage thérapeutique)</term>
<term>Chiens</term>
<term>Coronavirus canin ()</term>
<term>Fèces (virologie)</term>
<term>Humains</term>
<term>Indométacine (pharmacologie)</term>
<term>Indométacine (usage thérapeutique)</term>
<term>Infections à coronavirus (médecine vétérinaire)</term>
<term>Infections à coronavirus (traitement médicamenteux)</term>
<term>Interféron alpha (pharmacologie)</term>
<term>Lignée cellulaire</term>
<term>Maladies des chiens (traitement médicamenteux)</term>
<term>Relation dose-effet des médicaments</term>
<term>Ribavirine (pharmacologie)</term>
<term>Réplication virale ()</term>
<term>Virus du SRAS ()</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antiviral Agents</term>
<term>Aspirin</term>
<term>Indomethacin</term>
<term>Interferon-alpha</term>
<term>Ribavirin</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="therapeutic use" xml:lang="en"><term>Antiviral Agents</term>
<term>Indomethacin</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Coronavirus, Canine</term>
<term>SARS Virus</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="MESH" qualifier="drug therapy" xml:lang="en"><term>Coronavirus Infections</term>
<term>Dog Diseases</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ARN viral</term>
</keywords>
<keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Acide acétylsalicylique</term>
<term>Antiviraux</term>
<term>Indométacine</term>
<term>Interféron alpha</term>
<term>Ribavirine</term>
</keywords>
<keywords scheme="MESH" qualifier="traitement médicamenteux" xml:lang="fr"><term>Infections à coronavirus</term>
<term>Maladies des chiens</term>
</keywords>
<keywords scheme="MESH" qualifier="usage thérapeutique" xml:lang="fr"><term>Antiviraux</term>
<term>Indométacine</term>
</keywords>
<keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Coronavirus Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Fèces</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Feces</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cell Line</term>
<term>Chlorocebus aethiops</term>
<term>Dogs</term>
<term>Dose-Response Relationship, Drug</term>
<term>Humans</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Chiens</term>
<term>Coronavirus canin</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Relation dose-effet des médicaments</term>
<term>Réplication virale</term>
<term>Virus du SRAS</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is a newly emerging, highly transmissible and fatal disease caused by a previously unknown coronavirus (SARS-CoV). Existing in non-identified animal reservoirs, SARS-CoV continues to represent a threat to humans because there is no effective specific antiviral therapy for coronavirus infections.</div>
</front>
</TEI>
</PubMed>
</double>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004439 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 004439 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Main |étape= Curation |type= RBID |clé= Pascal:07-0058292 |texte= Indomethacin has a potent antiviral activity against SARS coronavirus }}
This area was generated with Dilib version V0.6.33. |