Quantitative temporal‐spatial distribution of severe acute respiratory syndrome‐associated coronavirus (SARS‐CoV) in post‐mortem tissues
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Auteurs : Julian W. Tang [République populaire de Chine] ; Ka-Fai To [République populaire de Chine] ; Anthony W. I. Lo [République populaire de Chine] ; Joseph J. Y. Sung [République populaire de Chine] ; H. K. Ng [République populaire de Chine] ; Paul K. S. Chan [République populaire de Chine, Hong Kong]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 2007-09.
English descriptors
- Teeft :
- Administrative region, Assay, Biopsy, Bowel, Cell culture, Chan, Chinese university, Coronavirus, Data point, Different organs, Different patient, Different patients, Ding, Farcas, Functional receptor, Gapdh, Hong kong, Infectious diseases, Lung liver right lung, Lung tissue, Noticeable drop, Pathol, Peiris, Postmortem, Psoas, Public health, Reaction volume, Receptor, Respiratory syndrome, Ribavirin, Sars, Sars coronavirus, Sars illness, Sars patients, Sarscov, Small bowel, Spleen, Syndrome, Tissue samples, Tropism, Viral, Viral load, Viral loads, Virol, Virus isolation, Wang, Wong, Zhang.
Abstract
Few post‐mortem studies have been performed on patients who have died from severe acute respiratory syndrome (SARS). No studies have examined how the SARS‐associated coronavirus (SARS‐CoV) loads in different organs with respect to time, post‐mortem. The aim of this study was to determine the quantitative temporal‐spatial distribution of SARS‐CoV in the post‐mortem tissue samples of seven patients. Quantitation of a house‐keeping gene, glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) was undertaken to standardize the amount of tissue tested. SARS‐CoV viral load and SARS‐CoV/GAPDH RNA ratio for each organ type were related to four time durations: onset of illness to death, death to post‐mortem tissue sampling, and total durations of treatment with ribavirin and hydrocortisone. The SARS‐CoV/GAPDH RNA ratio remained relatively stable in most organ tissue types for all these time durations. The ratio reached the highest value of equal to or greater than one for lung and small bowel, whereas those for heart, liver, spleen, and kidney were always less than one. It is concluded that SARS‐CoV viral loads in these organs remain relatively stable, post‐mortem. This quantitative assessment further supports SARS‐CoV has a specific tropism for the human respiratory and gastrointestinal tracts, which may be related to the density of SARS‐CoV receptors. J. Med. Virol. 79:1245–1253, 2007. © Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jmv.20873
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<front><div type="abstract" xml:lang="en">Few post‐mortem studies have been performed on patients who have died from severe acute respiratory syndrome (SARS). No studies have examined how the SARS‐associated coronavirus (SARS‐CoV) loads in different organs with respect to time, post‐mortem. The aim of this study was to determine the quantitative temporal‐spatial distribution of SARS‐CoV in the post‐mortem tissue samples of seven patients. Quantitation of a house‐keeping gene, glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) was undertaken to standardize the amount of tissue tested. SARS‐CoV viral load and SARS‐CoV/GAPDH RNA ratio for each organ type were related to four time durations: onset of illness to death, death to post‐mortem tissue sampling, and total durations of treatment with ribavirin and hydrocortisone. The SARS‐CoV/GAPDH RNA ratio remained relatively stable in most organ tissue types for all these time durations. The ratio reached the highest value of equal to or greater than one for lung and small bowel, whereas those for heart, liver, spleen, and kidney were always less than one. It is concluded that SARS‐CoV viral loads in these organs remain relatively stable, post‐mortem. This quantitative assessment further supports SARS‐CoV has a specific tropism for the human respiratory and gastrointestinal tracts, which may be related to the density of SARS‐CoV receptors. J. Med. Virol. 79:1245–1253, 2007. © Wiley‐Liss, Inc.</div>
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