Enhanced cell fusion activity in porcine epidemic diarrhea virus adapted to suckling mice
Identifieur interne : 000316 ( Istex/Curation ); précédent : 000315; suivant : 000317Enhanced cell fusion activity in porcine epidemic diarrhea virus adapted to suckling mice
Auteurs : Kazuya Shirato [Japon] ; Madoka Maejima [Japon] ; Asuka Hirai [Japon] ; Yasushi Ami [Japon] ; Natsumi Takeyama [Japon] ; Kotaro Tsuchiya [Japon] ; Kouich Kusanagi [Japon] ; Tetsuo Nunoya [Japon] ; Fumihiro Taguchi [Japon]Source :
- Archives of Virology [ 0304-8608 ] ; 2010-12-01.
Abstract
Abstract: Porcine epidemic diarrhea virus (PEDV) is the major causative agent of fatal diarrhea in piglets. To study the pathogenic features of PEDV using a mouse model, PEDV with virulence in mice is required. In pursuit of this, we adapted a tissue-culture-passed PEDV MK strain to suckling mouse brains. PEDV obtained after ten passages through the brains (MK-p10) had increased virulence for mice, and its fusion activity in cultured cells exceeded that of the original strain. However, the replication kinetics of MK and MK-p10 did not differ from each other in the brain and in cultured cells. The spike (S) protein of MK-p10 had four amino acid substitutions relative to the original strain. One of these (an H-to-R substitution at residue 1,381) was first detected in PEDV isolated after eight passages, and both this virus (MK-p8) and MK-p10 showed enhanced syncytium formation relative to the original MK strain and viruses isolated after two, four, and six passages, suggesting the possibility that the H-to-R mutation was responsible for this activity. This mutation could be also involved in the increased virulence of PEDV observed for MK-p10.
Url:
DOI: 10.1007/s00705-010-0790-1
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000316
Links to Exploration step
ISTEX:D5E3BA48C010EC6D50FF1930007DDA90DAE6AAC0Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Enhanced cell fusion activity in porcine epidemic diarrhea virus adapted to suckling mice</title>
<author><name sortKey="Shirato, Kazuya" sort="Shirato, Kazuya" uniqKey="Shirato K" first="Kazuya" last="Shirato">Kazuya Shirato</name>
<affiliation wicri:level="1"><mods:affiliation>Department of Virology III, Laboratory of Acute Respiratory Viral Diseases and Cytokines, Musashimurayama, Tokyo, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Virology III, Laboratory of Acute Respiratory Viral Diseases and Cytokines, Musashimurayama, Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Maejima, Madoka" sort="Maejima, Madoka" uniqKey="Maejima M" first="Madoka" last="Maejima">Madoka Maejima</name>
<affiliation wicri:level="1"><mods:affiliation>Department of Virology III, Laboratory of Acute Respiratory Viral Diseases and Cytokines, Musashimurayama, Tokyo, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Virology III, Laboratory of Acute Respiratory Viral Diseases and Cytokines, Musashimurayama, Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Hirai, Asuka" sort="Hirai, Asuka" uniqKey="Hirai A" first="Asuka" last="Hirai">Asuka Hirai</name>
<affiliation wicri:level="1"><mods:affiliation>Division of Experimental Animals Research, National Institute of Infectious Diseases, 4-7-1 Gakuen, 208-0011, Musashimurayama, Tokyo, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Division of Experimental Animals Research, National Institute of Infectious Diseases, 4-7-1 Gakuen, 208-0011, Musashimurayama, Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ami, Yasushi" sort="Ami, Yasushi" uniqKey="Ami Y" first="Yasushi" last="Ami">Yasushi Ami</name>
<affiliation wicri:level="1"><mods:affiliation>Division of Experimental Animals Research, National Institute of Infectious Diseases, 4-7-1 Gakuen, 208-0011, Musashimurayama, Tokyo, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Division of Experimental Animals Research, National Institute of Infectious Diseases, 4-7-1 Gakuen, 208-0011, Musashimurayama, Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Takeyama, Natsumi" sort="Takeyama, Natsumi" uniqKey="Takeyama N" first="Natsumi" last="Takeyama">Natsumi Takeyama</name>
<affiliation wicri:level="1"><mods:affiliation>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Tsuchiya, Kotaro" sort="Tsuchiya, Kotaro" uniqKey="Tsuchiya K" first="Kotaro" last="Tsuchiya">Kotaro Tsuchiya</name>
<affiliation wicri:level="1"><mods:affiliation>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kusanagi, Kouich" sort="Kusanagi, Kouich" uniqKey="Kusanagi K" first="Kouich" last="Kusanagi">Kouich Kusanagi</name>
<affiliation wicri:level="1"><mods:affiliation>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Nunoya, Tetsuo" sort="Nunoya, Tetsuo" uniqKey="Nunoya T" first="Tetsuo" last="Nunoya">Tetsuo Nunoya</name>
<affiliation wicri:level="1"><mods:affiliation>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name>
<affiliation wicri:level="1"><mods:affiliation>Department of Virology III, Laboratory of Acute Respiratory Viral Diseases and Cytokines, Musashimurayama, Tokyo, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Virology III, Laboratory of Acute Respiratory Viral Diseases and Cytokines, Musashimurayama, Tokyo</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Faculty of Veterinary Medicine, Laboratory of Virology and Viral Infections, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, 180-8602, Musashino, Tokyo, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Faculty of Veterinary Medicine, Laboratory of Virology and Viral Infections, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, 180-8602, Musashino, Tokyo</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>E-mail: ftaguchi@nvlu.ac.jp</mods:affiliation>
<country wicri:rule="url">Japon</country>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:D5E3BA48C010EC6D50FF1930007DDA90DAE6AAC0</idno>
<date when="2010" year="2010">2010</date>
<idno type="doi">10.1007/s00705-010-0790-1</idno>
<idno type="url">https://api.istex.fr/ark:/67375/VQC-QXGSRC8S-K/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000316</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000316</idno>
<idno type="wicri:Area/Istex/Curation">000316</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Enhanced cell fusion activity in porcine epidemic diarrhea virus adapted to suckling mice</title>
<author><name sortKey="Shirato, Kazuya" sort="Shirato, Kazuya" uniqKey="Shirato K" first="Kazuya" last="Shirato">Kazuya Shirato</name>
<affiliation wicri:level="1"><mods:affiliation>Department of Virology III, Laboratory of Acute Respiratory Viral Diseases and Cytokines, Musashimurayama, Tokyo, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Virology III, Laboratory of Acute Respiratory Viral Diseases and Cytokines, Musashimurayama, Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Maejima, Madoka" sort="Maejima, Madoka" uniqKey="Maejima M" first="Madoka" last="Maejima">Madoka Maejima</name>
<affiliation wicri:level="1"><mods:affiliation>Department of Virology III, Laboratory of Acute Respiratory Viral Diseases and Cytokines, Musashimurayama, Tokyo, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Virology III, Laboratory of Acute Respiratory Viral Diseases and Cytokines, Musashimurayama, Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Hirai, Asuka" sort="Hirai, Asuka" uniqKey="Hirai A" first="Asuka" last="Hirai">Asuka Hirai</name>
<affiliation wicri:level="1"><mods:affiliation>Division of Experimental Animals Research, National Institute of Infectious Diseases, 4-7-1 Gakuen, 208-0011, Musashimurayama, Tokyo, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Division of Experimental Animals Research, National Institute of Infectious Diseases, 4-7-1 Gakuen, 208-0011, Musashimurayama, Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ami, Yasushi" sort="Ami, Yasushi" uniqKey="Ami Y" first="Yasushi" last="Ami">Yasushi Ami</name>
<affiliation wicri:level="1"><mods:affiliation>Division of Experimental Animals Research, National Institute of Infectious Diseases, 4-7-1 Gakuen, 208-0011, Musashimurayama, Tokyo, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Division of Experimental Animals Research, National Institute of Infectious Diseases, 4-7-1 Gakuen, 208-0011, Musashimurayama, Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Takeyama, Natsumi" sort="Takeyama, Natsumi" uniqKey="Takeyama N" first="Natsumi" last="Takeyama">Natsumi Takeyama</name>
<affiliation wicri:level="1"><mods:affiliation>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Tsuchiya, Kotaro" sort="Tsuchiya, Kotaro" uniqKey="Tsuchiya K" first="Kotaro" last="Tsuchiya">Kotaro Tsuchiya</name>
<affiliation wicri:level="1"><mods:affiliation>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kusanagi, Kouich" sort="Kusanagi, Kouich" uniqKey="Kusanagi K" first="Kouich" last="Kusanagi">Kouich Kusanagi</name>
<affiliation wicri:level="1"><mods:affiliation>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Nunoya, Tetsuo" sort="Nunoya, Tetsuo" uniqKey="Nunoya T" first="Tetsuo" last="Nunoya">Tetsuo Nunoya</name>
<affiliation wicri:level="1"><mods:affiliation>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name>
<affiliation wicri:level="1"><mods:affiliation>Department of Virology III, Laboratory of Acute Respiratory Viral Diseases and Cytokines, Musashimurayama, Tokyo, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Virology III, Laboratory of Acute Respiratory Viral Diseases and Cytokines, Musashimurayama, Tokyo</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Faculty of Veterinary Medicine, Laboratory of Virology and Viral Infections, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, 180-8602, Musashino, Tokyo, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Faculty of Veterinary Medicine, Laboratory of Virology and Viral Infections, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, 180-8602, Musashino, Tokyo</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>E-mail: ftaguchi@nvlu.ac.jp</mods:affiliation>
<country wicri:rule="url">Japon</country>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Archives of Virology</title>
<title level="j" type="sub">Official Journal of the Virology Division of the International Union of Microbiological Societies</title>
<title level="j" type="abbrev">Arch Virol</title>
<idno type="ISSN">0304-8608</idno>
<idno type="eISSN">1432-8798</idno>
<imprint><publisher>Springer Vienna</publisher>
<pubPlace>Vienna</pubPlace>
<date type="published" when="2010-12-01">2010-12-01</date>
<biblScope unit="volume">155</biblScope>
<biblScope unit="issue">12</biblScope>
<biblScope unit="page" from="1989">1989</biblScope>
<biblScope unit="page" to="1995">1995</biblScope>
</imprint>
<idno type="ISSN">0304-8608</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0304-8608</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: Porcine epidemic diarrhea virus (PEDV) is the major causative agent of fatal diarrhea in piglets. To study the pathogenic features of PEDV using a mouse model, PEDV with virulence in mice is required. In pursuit of this, we adapted a tissue-culture-passed PEDV MK strain to suckling mouse brains. PEDV obtained after ten passages through the brains (MK-p10) had increased virulence for mice, and its fusion activity in cultured cells exceeded that of the original strain. However, the replication kinetics of MK and MK-p10 did not differ from each other in the brain and in cultured cells. The spike (S) protein of MK-p10 had four amino acid substitutions relative to the original strain. One of these (an H-to-R substitution at residue 1,381) was first detected in PEDV isolated after eight passages, and both this virus (MK-p8) and MK-p10 showed enhanced syncytium formation relative to the original MK strain and viruses isolated after two, four, and six passages, suggesting the possibility that the H-to-R mutation was responsible for this activity. This mutation could be also involved in the increased virulence of PEDV observed for MK-p10.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000316 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 000316 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Istex |étape= Curation |type= RBID |clé= ISTEX:D5E3BA48C010EC6D50FF1930007DDA90DAE6AAC0 |texte= Enhanced cell fusion activity in porcine epidemic diarrhea virus adapted to suckling mice }}
This area was generated with Dilib version V0.6.33. |