Enhanced cell fusion activity in porcine epidemic diarrhea virus adapted to suckling mice
Identifieur interne : 000316 ( Istex/Corpus ); précédent : 000315; suivant : 000317Enhanced cell fusion activity in porcine epidemic diarrhea virus adapted to suckling mice
Auteurs : Kazuya Shirato ; Madoka Maejima ; Asuka Hirai ; Yasushi Ami ; Natsumi Takeyama ; Kotaro Tsuchiya ; Kouich Kusanagi ; Tetsuo Nunoya ; Fumihiro TaguchiSource :
- Archives of Virology [ 0304-8608 ] ; 2010-12-01.
Abstract
Abstract: Porcine epidemic diarrhea virus (PEDV) is the major causative agent of fatal diarrhea in piglets. To study the pathogenic features of PEDV using a mouse model, PEDV with virulence in mice is required. In pursuit of this, we adapted a tissue-culture-passed PEDV MK strain to suckling mouse brains. PEDV obtained after ten passages through the brains (MK-p10) had increased virulence for mice, and its fusion activity in cultured cells exceeded that of the original strain. However, the replication kinetics of MK and MK-p10 did not differ from each other in the brain and in cultured cells. The spike (S) protein of MK-p10 had four amino acid substitutions relative to the original strain. One of these (an H-to-R substitution at residue 1,381) was first detected in PEDV isolated after eight passages, and both this virus (MK-p8) and MK-p10 showed enhanced syncytium formation relative to the original MK strain and viruses isolated after two, four, and six passages, suggesting the possibility that the H-to-R mutation was responsible for this activity. This mutation could be also involved in the increased virulence of PEDV observed for MK-p10.
Url:
DOI: 10.1007/s00705-010-0790-1
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cell fusion activity in porcine epidemic diarrhea virus adapted to suckling mice</title><author><name sortKey="Shirato, Kazuya" sort="Shirato, Kazuya" uniqKey="Shirato K" first="Kazuya" last="Shirato">Kazuya Shirato</name><affiliation><mods:affiliation>Department of Virology III, Laboratory of Acute Respiratory Viral Diseases and Cytokines, Musashimurayama, Tokyo, Japan</mods:affiliation></affiliation></author><author><name sortKey="Maejima, Madoka" sort="Maejima, Madoka" uniqKey="Maejima M" first="Madoka" last="Maejima">Madoka Maejima</name><affiliation><mods:affiliation>Department of Virology III, Laboratory of Acute Respiratory Viral Diseases and Cytokines, Musashimurayama, Tokyo, Japan</mods:affiliation></affiliation></author><author><name sortKey="Hirai, Asuka" sort="Hirai, Asuka" uniqKey="Hirai A" first="Asuka" last="Hirai">Asuka Hirai</name><affiliation><mods:affiliation>Division of Experimental Animals Research, National Institute of Infectious Diseases, 4-7-1 Gakuen, 208-0011, Musashimurayama, Tokyo, Japan</mods:affiliation></affiliation></author><author><name sortKey="Ami, Yasushi" sort="Ami, Yasushi" uniqKey="Ami Y" first="Yasushi" last="Ami">Yasushi Ami</name><affiliation><mods:affiliation>Division of Experimental Animals Research, National Institute of Infectious Diseases, 4-7-1 Gakuen, 208-0011, Musashimurayama, Tokyo, Japan</mods:affiliation></affiliation></author><author><name sortKey="Takeyama, Natsumi" sort="Takeyama, Natsumi" uniqKey="Takeyama N" first="Natsumi" last="Takeyama">Natsumi Takeyama</name><affiliation><mods:affiliation>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo, Japan</mods:affiliation></affiliation></author><author><name sortKey="Tsuchiya, Kotaro" sort="Tsuchiya, Kotaro" uniqKey="Tsuchiya K" first="Kotaro" last="Tsuchiya">Kotaro Tsuchiya</name><affiliation><mods:affiliation>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo, 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To study the pathogenic features of PEDV using a mouse model, PEDV with virulence in mice is required. In pursuit of this, we adapted a tissue-culture-passed PEDV MK strain to suckling mouse brains. PEDV obtained after ten passages through the brains (MK-p10) had increased virulence for mice, and its fusion activity in cultured cells exceeded that of the original strain. However, the replication kinetics of MK and MK-p10 did not differ from each other in the brain and in cultured cells. The spike (S) protein of MK-p10 had four amino acid substitutions relative to the original strain. One of these (an H-to-R substitution at residue 1,381) was first detected in PEDV isolated after eight passages, and both this virus (MK-p8) and MK-p10 showed enhanced syncytium formation relative to the original MK strain and viruses isolated after two, four, and six passages, suggesting the possibility that the H-to-R mutation was responsible for this activity. This mutation could be also involved in the increased virulence of PEDV observed for MK-p10.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>Kazuya Shirato</name><affiliations><json:string>Department of Virology III, Laboratory of Acute Respiratory Viral Diseases and Cytokines, Musashimurayama, Tokyo, Japan</json:string></affiliations></json:item><json:item><name>Madoka Maejima</name><affiliations><json:string>Department of Virology III, Laboratory of Acute Respiratory Viral Diseases and Cytokines, Musashimurayama, Tokyo, Japan</json:string></affiliations></json:item><json:item><name>Asuka Hirai</name><affiliations><json:string>Division of Experimental Animals Research, National Institute of Infectious Diseases, 4-7-1 Gakuen, 208-0011, Musashimurayama, Tokyo, Japan</json:string></affiliations></json:item><json:item><name>Yasushi Ami</name><affiliations><json:string>Division of Experimental Animals Research, National Institute of Infectious Diseases, 4-7-1 Gakuen, 208-0011, Musashimurayama, Tokyo, Japan</json:string></affiliations></json:item><json:item><name>Natsumi Takeyama</name><affiliations><json:string>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo, Japan</json:string></affiliations></json:item><json:item><name>Kotaro Tsuchiya</name><affiliations><json:string>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo, Japan</json:string></affiliations></json:item><json:item><name>Kouich Kusanagi</name><affiliations><json:string>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo, Japan</json:string></affiliations></json:item><json:item><name>Tetsuo Nunoya</name><affiliations><json:string>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo, Japan</json:string></affiliations></json:item><json:item><name>Fumihiro Taguchi</name><affiliations><json:string>Department of Virology III, Laboratory of Acute Respiratory Viral Diseases and Cytokines, Musashimurayama, Tokyo, Japan</json:string><json:string>Faculty of Veterinary Medicine, Laboratory of Virology and Viral Infections, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, 180-8602, Musashino, Tokyo, Japan</json:string><json:string>E-mail: ftaguchi@nvlu.ac.jp</json:string></affiliations></json:item></author><articleId><json:string>790</json:string><json:string>s00705-010-0790-1</json:string></articleId><arkIstex>ark:/67375/VQC-QXGSRC8S-K</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: Porcine epidemic diarrhea virus (PEDV) is the major causative agent of fatal diarrhea in piglets. To study the pathogenic features of PEDV using a mouse model, PEDV with virulence in mice is required. In pursuit of this, we adapted a tissue-culture-passed PEDV MK strain to suckling mouse brains. PEDV obtained after ten passages through the brains (MK-p10) had increased virulence for mice, and its fusion activity in cultured cells exceeded that of the original strain. However, the replication kinetics of MK and MK-p10 did not differ from each other in the brain and in cultured cells. The spike (S) protein of MK-p10 had four amino acid substitutions relative to the original strain. One of these (an H-to-R substitution at residue 1,381) was first detected in PEDV isolated after eight passages, and both this virus (MK-p8) and MK-p10 showed enhanced syncytium formation relative to the original MK strain and viruses isolated after two, four, and six passages, suggesting the possibility that the H-to-R mutation was responsible for this activity. 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To study the pathogenic features of PEDV using a mouse model, PEDV with virulence in mice is required. In pursuit of this, we adapted a tissue-culture-passed PEDV MK strain to suckling mouse brains. PEDV obtained after ten passages through the brains (MK-p10) had increased virulence for mice, and its fusion activity in cultured cells exceeded that of the original strain. However, the replication kinetics of MK and MK-p10 did not differ from each other in the brain and in cultured cells. The spike (S) protein of MK-p10 had four amino acid substitutions relative to the original strain. One of these (an H-to-R substitution at residue 1,381) was first detected in PEDV isolated after eight passages, and both this virus (MK-p8) and MK-p10 showed enhanced syncytium formation relative to the original MK strain and viruses isolated after two, four, and six passages, suggesting the possibility that the H-to-R mutation was responsible for this activity. This mutation could be also involved in the increased virulence of PEDV observed for MK-p10.</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>Biomedicine</head><item><term>Infectious Diseases</term></item><item><term>Medical Microbiology</term></item><item><term>Virology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2010-02-09">Created</change><change when="2010-12-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-QXGSRC8S-K/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" 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TocLevels="0"><ArticleID>790</ArticleID><ArticleDOI>10.1007/s00705-010-0790-1</ArticleDOI><ArticleSequenceNumber>8</ArticleSequenceNumber><ArticleTitle Language="En" OutputMedium="All">Enhanced cell fusion activity in porcine epidemic diarrhea virus adapted to suckling mice</ArticleTitle><ArticleCategory>Original Article</ArticleCategory><ArticleFirstPage>1989</ArticleFirstPage><ArticleLastPage>1995</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2010</Year><Month>8</Month><Day>30</Day></RegistrationDate><Received><Year>2010</Year><Month>2</Month><Day>9</Day></Received><Accepted><Year>2010</Year><Month>8</Month><Day>27</Day></Accepted><OnlineDate><Year>2010</Year><Month>9</Month><Day>9</Day></OnlineDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>2010</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Kazuya</GivenName><FamilyName>Shirato</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Madoka</GivenName><FamilyName>Maejima</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Asuka</GivenName><FamilyName>Hirai</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Yasushi</GivenName><FamilyName>Ami</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff3"><AuthorName DisplayOrder="Western"><GivenName>Natsumi</GivenName><FamilyName>Takeyama</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff3"><AuthorName DisplayOrder="Western"><GivenName>Kotaro</GivenName><FamilyName>Tsuchiya</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff3"><AuthorName DisplayOrder="Western"><GivenName>Kouich</GivenName><FamilyName>Kusanagi</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff3"><AuthorName DisplayOrder="Western"><GivenName>Tetsuo</GivenName><FamilyName>Nunoya</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff4" CorrespondingAffiliationID="Aff4"><AuthorName DisplayOrder="Western"><GivenName>Fumihiro</GivenName><FamilyName>Taguchi</FamilyName></AuthorName><Contact><Phone>+81-422-314151</Phone><Fax>+81-422-332094</Fax><Email>ftaguchi@nvlu.ac.jp</Email></Contact></Author><Affiliation ID="Aff1"><OrgDivision>Department of Virology III</OrgDivision><OrgName>Laboratory of Acute Respiratory Viral Diseases and Cytokines</OrgName><OrgAddress><City>Musashimurayama</City><State>Tokyo</State><Country Code="JP">Japan</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Division of Experimental Animals Research</OrgDivision><OrgName>National Institute of Infectious Diseases</OrgName><OrgAddress><Street>4-7-1 Gakuen</Street><City>Musashimurayama</City><State>Tokyo</State><Postcode>208-0011</Postcode><Country Code="JP">Japan</Country></OrgAddress></Affiliation><Affiliation ID="Aff3"><OrgName>Nippon Institute for Biological Science</OrgName><OrgAddress><Street>9-2222-1 Shinmachi</Street><City>Ome</City><State>Tokyo</State><Postcode>198-0024</Postcode><Country Code="JP">Japan</Country></OrgAddress></Affiliation><Affiliation ID="Aff4"><OrgDivision>Faculty of Veterinary Medicine, Laboratory of Virology and Viral Infections</OrgDivision><OrgName>Nippon Veterinary and Life Science University</OrgName><OrgAddress><Street>1-7-1 Kyonan-cho</Street><City>Musashino</City><State>Tokyo</State><Postcode>180-8602</Postcode><Country Code="JP">Japan</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En" OutputMedium="All"><Heading>Abstract</Heading><Para>Porcine epidemic diarrhea virus (PEDV) is the major causative agent of fatal diarrhea in piglets. To study the pathogenic features of PEDV using a mouse model, PEDV with virulence in mice is required. In pursuit of this, we adapted a tissue-culture-passed PEDV MK strain to suckling mouse brains. PEDV obtained after ten passages through the brains (MK-p10) had increased virulence for mice, and its fusion activity in cultured cells exceeded that of the original strain. However, the replication kinetics of MK and MK-p10 did not differ from each other in the brain and in cultured cells. The spike (S) protein of MK-p10 had four amino acid substitutions relative to the original strain. One of these (an H-to-R substitution at residue 1,381) was first detected in PEDV isolated after eight passages, and both this virus (MK-p8) and MK-p10 showed enhanced syncytium formation relative to the original MK strain and viruses isolated after two, four, and six passages, suggesting the possibility that the H-to-R mutation was responsible for this activity. This mutation could be also involved in the increased virulence of PEDV observed for MK-p10.</Para></Abstract><AbbreviationGroup><Heading>Abbreviations</Heading><DefinitionList><DefinitionListEntry><Term>APN</Term><Description><Para>Aminopeptidase N</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>CT</Term><Description><Para>Cytoplasmic tail</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>FCS</Term><Description><Para>Fetal calf serum</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>DMEM</Term><Description><Para>Dulbecco’s modified Eagle’s medium</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>HCoV</Term><Description><Para>Human coronavirus</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>IBV</Term><Description><Para>Infectious bronchitis virus</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>i.c.</Term><Description><Para>Intracerebrally</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>MHV</Term><Description><Para>Mouse hepatitis virus</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>m.o.i.</Term><Description><Para>Multiplicity of infection</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>PEDV</Term><Description><Para>Porcine epidemic diarrhea virus</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>PBS</Term><Description><Para>Phosphate-buffered saline</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>PFU</Term><Description><Para>Plaque-forming unit</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>p.i.</Term><Description><Para>Post-infection</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>S</Term><Description><Para>Spike</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>SARS</Term><Description><Para>Severe acute respiratory syndrome</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>TGEV</Term><Description><Para>Transmissible gastroenteritis virus</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>TPB</Term><Description><Para>Tryptose phosphate broth</Para></Description></DefinitionListEntry></DefinitionList></AbbreviationGroup></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Enhanced cell fusion activity in porcine epidemic diarrhea virus adapted to suckling mice</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Enhanced cell fusion activity in porcine epidemic diarrhea virus adapted to suckling mice</title></titleInfo><name type="personal"><namePart type="given">Kazuya</namePart><namePart type="family">Shirato</namePart><affiliation>Department of Virology III, Laboratory of Acute Respiratory Viral Diseases and Cytokines, Musashimurayama, Tokyo, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Madoka</namePart><namePart type="family">Maejima</namePart><affiliation>Department of Virology III, Laboratory of Acute Respiratory Viral Diseases and Cytokines, Musashimurayama, Tokyo, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Asuka</namePart><namePart type="family">Hirai</namePart><affiliation>Division of Experimental Animals Research, National Institute of Infectious Diseases, 4-7-1 Gakuen, 208-0011, Musashimurayama, Tokyo, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yasushi</namePart><namePart type="family">Ami</namePart><affiliation>Division of Experimental Animals Research, National Institute of Infectious Diseases, 4-7-1 Gakuen, 208-0011, Musashimurayama, Tokyo, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Natsumi</namePart><namePart type="family">Takeyama</namePart><affiliation>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Kotaro</namePart><namePart type="family">Tsuchiya</namePart><affiliation>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Kouich</namePart><namePart type="family">Kusanagi</namePart><affiliation>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Tetsuo</namePart><namePart type="family">Nunoya</namePart><affiliation>Nippon Institute for Biological Science, 9-2222-1 Shinmachi, 198-0024, Ome, Tokyo, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Fumihiro</namePart><namePart type="family">Taguchi</namePart><affiliation>Department of Virology III, Laboratory of Acute Respiratory Viral Diseases and Cytokines, Musashimurayama, Tokyo, Japan</affiliation><affiliation>Faculty of Veterinary Medicine, Laboratory of Virology and Viral Infections, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, 180-8602, Musashino, Tokyo, Japan</affiliation><affiliation>E-mail: ftaguchi@nvlu.ac.jp</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer Vienna</publisher><place><placeTerm type="text">Vienna</placeTerm></place><dateCreated encoding="w3cdtf">2010-02-09</dateCreated><dateIssued encoding="w3cdtf">2010-12-01</dateIssued><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: Porcine epidemic diarrhea virus (PEDV) is the major causative agent of fatal diarrhea in piglets. To study the pathogenic features of PEDV using a mouse model, PEDV with virulence in mice is required. In pursuit of this, we adapted a tissue-culture-passed PEDV MK strain to suckling mouse brains. PEDV obtained after ten passages through the brains (MK-p10) had increased virulence for mice, and its fusion activity in cultured cells exceeded that of the original strain. However, the replication kinetics of MK and MK-p10 did not differ from each other in the brain and in cultured cells. The spike (S) protein of MK-p10 had four amino acid substitutions relative to the original strain. One of these (an H-to-R substitution at residue 1,381) was first detected in PEDV isolated after eight passages, and both this virus (MK-p8) and MK-p10 showed enhanced syncytium formation relative to the original MK strain and viruses isolated after two, four, and six passages, suggesting the possibility that the H-to-R mutation was responsible for this activity. This mutation could be also involved in the increased virulence of PEDV observed for MK-p10.</abstract><note>Original Article</note><relatedItem type="host"><titleInfo><title>Archives of Virology</title><subTitle>Official Journal of the Virology Division of the International Union of Microbiological Societies</subTitle></titleInfo><titleInfo type="abbreviated"><title>Arch Virol</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2010-11-17</dateIssued><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><subject><genre>Biomedicine</genre><topic>Infectious Diseases</topic><topic>Medical Microbiology</topic><topic>Virology</topic></subject><identifier type="ISSN">0304-8608</identifier><identifier type="eISSN">1432-8798</identifier><identifier type="JournalID">705</identifier><identifier type="IssueArticleCount">23</identifier><identifier type="VolumeIssueCount">12</identifier><part><date>2010</date><detail type="volume"><number>155</number><caption>vol.</caption></detail><detail type="issue"><number>12</number><caption>no.</caption></detail><extent unit="pages"><start>1989</start><end>1995</end></extent></part><recordInfo><recordOrigin>Springer-Verlag, 2010</recordOrigin></recordInfo></relatedItem><identifier type="istex">D5E3BA48C010EC6D50FF1930007DDA90DAE6AAC0</identifier><identifier type="ark">ark:/67375/VQC-QXGSRC8S-K</identifier><identifier type="DOI">10.1007/s00705-010-0790-1</identifier><identifier type="ArticleID">790</identifier><identifier type="ArticleID">s00705-010-0790-1</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer-Verlag, 2010</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Springer-Verlag, 2010</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-QXGSRC8S-K/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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