Use of virus vectors for the expression in plants of active full‐length and single chain anti‐coronavirus antibodies
Identifieur interne : 001888 ( Istex/Corpus ); précédent : 001887; suivant : 001889Use of virus vectors for the expression in plants of active full‐length and single chain anti‐coronavirus antibodies
Auteurs : Josefa M. Alamillo ; Wendy Monger ; Isabel Sola ; Beatriz García ; Yolande Perrin ; Marco Bestagno ; Oscar R. Burrone ; Patricia Sabella ; Joan Plana-Durán ; Luis Enjuanes ; George P. Lomonossoff ; Juan A. GarcíaSource :
- Biotechnology Journal [ 1860-6768 ] ; 2006-10.
Abstract
To extend the potential of antibodies and their derivatives to provide passive protection against enteric infections when supplied orally in crude plant extracts, we have expressed both a small immune protein (SIP) and a full‐length antibody in plants using two different plant virus vectors based on potato virus X (PVX) and cowpea mosaic virus (CPMV). The agr;SIP molecule consisted of a single chain antibody (scFv) specific for the porcine coronavirus, transmissible gastroenteritis virus (TGEV) linked to the α‐CH3 domain from human IgA. To express the full‐length IgA, the individual light and heavy chains from the TGEV‐specific mAb 6A.C3 were inserted into separate PVX constructs and plants were co‐infected with both constructs. Western blot analysis revealed the efficient expression of both the SIP and IgA molecules. Analysis of crude plant extracts revealed that both the plant‐expressed αSIP and IgA molecules could bind to and neutralize TGEV in tissue culture, indicating that active molecules were produced. Oral administration of crude extracts from antibody‐expressing plant tissue to 2‐day‐old piglets showed that both the αSIP and full‐length IgA molecules can provide in vivo protection against TGEV.
Url:
DOI: 10.1002/biot.200600143
Links to Exploration step
ISTEX:271923F222E75015A690589A822585977821BEC2Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Use of virus vectors for the expression in plants of active full‐length and single chain anti‐coronavirus antibodies</title><author><name sortKey="Alamillo, Josefa M" sort="Alamillo, Josefa M" uniqKey="Alamillo J" first="Josefa M." last="Alamillo">Josefa M. Alamillo</name><affiliation><mods:affiliation>Centro Nacional de Biotecnología, Campus Universidad Autónoma, Madrid, Spain</mods:affiliation></affiliation><affiliation><mods:affiliation>Universidad de Córdoba, Campus Rabanales, C‐6, 14071 Córdoba, Spain</mods:affiliation></affiliation></author><author><name sortKey="Monger, Wendy" sort="Monger, Wendy" uniqKey="Monger W" first="Wendy" last="Monger">Wendy Monger</name><affiliation><mods:affiliation>John Innes Centre, Norwich, UK</mods:affiliation></affiliation></author><author><name sortKey="Sola, Isabel" sort="Sola, Isabel" uniqKey="Sola I" first="Isabel" last="Sola">Isabel Sola</name><affiliation><mods:affiliation>Centro Nacional de Biotecnología, Campus Universidad Autónoma, Madrid, Spain</mods:affiliation></affiliation></author><author><name sortKey="Garcia, Beatriz" sort="Garcia, Beatriz" uniqKey="Garcia B" first="Beatriz" last="García">Beatriz García</name><affiliation><mods:affiliation>Centro Nacional de Biotecnología, Campus Universidad Autónoma, Madrid, Spain</mods:affiliation></affiliation></author><author><name sortKey="Perrin, Yolande" sort="Perrin, Yolande" uniqKey="Perrin Y" first="Yolande" last="Perrin">Yolande Perrin</name><affiliation><mods:affiliation>John Innes Centre, Norwich, UK</mods:affiliation></affiliation><affiliation><mods:affiliation>Universitéde Technologie de Compiègne, BP 20529, 60205 Compiègne Cédex, France</mods:affiliation></affiliation></author><author><name sortKey="Bestagno, Marco" sort="Bestagno, Marco" uniqKey="Bestagno M" first="Marco" last="Bestagno">Marco Bestagno</name><affiliation><mods:affiliation>International Center for Genetic Engineering and Biotechnology, Trieste, Italy</mods:affiliation></affiliation></author><author><name sortKey="Burrone, Oscar R" sort="Burrone, Oscar R" uniqKey="Burrone O" first="Oscar R." last="Burrone">Oscar R. Burrone</name><affiliation><mods:affiliation>International Center for Genetic Engineering and Biotechnology, Trieste, Italy</mods:affiliation></affiliation></author><author><name sortKey="Sabella, Patricia" sort="Sabella, Patricia" uniqKey="Sabella P" first="Patricia" last="Sabella">Patricia Sabella</name><affiliation><mods:affiliation>Fort Dodge Veterinaria SA, Carretera de Comprodon, Girona, Spain</mods:affiliation></affiliation></author><author><name sortKey="Plana Uran, Joan" sort="Plana Uran, Joan" uniqKey="Plana Uran J" first="Joan" last="Plana-Durán">Joan Plana-Durán</name><affiliation><mods:affiliation>Fort Dodge Veterinaria SA, Carretera de Comprodon, Girona, Spain</mods:affiliation></affiliation></author><author><name sortKey="Enjuanes, Luis" sort="Enjuanes, Luis" uniqKey="Enjuanes L" first="Luis" last="Enjuanes">Luis Enjuanes</name><affiliation><mods:affiliation>Centro Nacional de Biotecnología, Campus Universidad Autónoma, Madrid, Spain</mods:affiliation></affiliation></author><author><name sortKey="Lomonossoff, George P" sort="Lomonossoff, George P" uniqKey="Lomonossoff G" first="George P." last="Lomonossoff">George P. Lomonossoff</name><affiliation><mods:affiliation>John Innes Centre, Norwich, UK</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: george.lomonossoff@bbsrc.ac.uk</mods:affiliation></affiliation><affiliation><mods:affiliation>Correspondence address: John Innes Centre, Colney lane, Norwich NR4 7UH, UK, +44‐1603‐450018</mods:affiliation></affiliation></author><author><name sortKey="Garcia, Juan A" sort="Garcia, Juan A" uniqKey="Garcia J" first="Juan A." last="García">Juan A. García</name><affiliation><mods:affiliation>Centro Nacional de Biotecnología, Campus Universidad Autónoma, Madrid, Spain</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:271923F222E75015A690589A822585977821BEC2</idno><date when="2006" year="2006">2006</date><idno type="doi">10.1002/biot.200600143</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-4H4VNSSX-D/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001888</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001888</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Use of virus vectors for the expression in plants of active full‐length and single chain anti‐coronavirus antibodies</title><author><name sortKey="Alamillo, Josefa M" sort="Alamillo, Josefa M" uniqKey="Alamillo J" first="Josefa M." last="Alamillo">Josefa M. Alamillo</name><affiliation><mods:affiliation>Centro Nacional de Biotecnología, Campus Universidad Autónoma, Madrid, Spain</mods:affiliation></affiliation><affiliation><mods:affiliation>Universidad de Córdoba, Campus Rabanales, C‐6, 14071 Córdoba, Spain</mods:affiliation></affiliation></author><author><name sortKey="Monger, Wendy" sort="Monger, Wendy" uniqKey="Monger W" first="Wendy" last="Monger">Wendy Monger</name><affiliation><mods:affiliation>John Innes Centre, Norwich, UK</mods:affiliation></affiliation></author><author><name sortKey="Sola, Isabel" sort="Sola, Isabel" uniqKey="Sola I" first="Isabel" last="Sola">Isabel Sola</name><affiliation><mods:affiliation>Centro Nacional de Biotecnología, Campus Universidad Autónoma, Madrid, Spain</mods:affiliation></affiliation></author><author><name sortKey="Garcia, Beatriz" sort="Garcia, Beatriz" uniqKey="Garcia B" first="Beatriz" last="García">Beatriz García</name><affiliation><mods:affiliation>Centro Nacional de Biotecnología, Campus Universidad Autónoma, Madrid, Spain</mods:affiliation></affiliation></author><author><name sortKey="Perrin, Yolande" sort="Perrin, Yolande" uniqKey="Perrin Y" first="Yolande" last="Perrin">Yolande Perrin</name><affiliation><mods:affiliation>John Innes Centre, Norwich, UK</mods:affiliation></affiliation><affiliation><mods:affiliation>Universitéde Technologie de Compiègne, BP 20529, 60205 Compiègne Cédex, France</mods:affiliation></affiliation></author><author><name sortKey="Bestagno, Marco" sort="Bestagno, Marco" uniqKey="Bestagno M" first="Marco" last="Bestagno">Marco Bestagno</name><affiliation><mods:affiliation>International Center for Genetic Engineering and Biotechnology, Trieste, Italy</mods:affiliation></affiliation></author><author><name sortKey="Burrone, Oscar R" sort="Burrone, Oscar R" uniqKey="Burrone O" first="Oscar R." last="Burrone">Oscar R. Burrone</name><affiliation><mods:affiliation>International Center for Genetic Engineering and Biotechnology, Trieste, Italy</mods:affiliation></affiliation></author><author><name sortKey="Sabella, Patricia" sort="Sabella, Patricia" uniqKey="Sabella P" first="Patricia" last="Sabella">Patricia Sabella</name><affiliation><mods:affiliation>Fort Dodge Veterinaria SA, Carretera de Comprodon, Girona, Spain</mods:affiliation></affiliation></author><author><name sortKey="Plana Uran, Joan" sort="Plana Uran, Joan" uniqKey="Plana Uran J" first="Joan" last="Plana-Durán">Joan Plana-Durán</name><affiliation><mods:affiliation>Fort Dodge Veterinaria SA, Carretera de Comprodon, Girona, Spain</mods:affiliation></affiliation></author><author><name sortKey="Enjuanes, Luis" sort="Enjuanes, Luis" uniqKey="Enjuanes L" first="Luis" last="Enjuanes">Luis Enjuanes</name><affiliation><mods:affiliation>Centro Nacional de Biotecnología, Campus Universidad Autónoma, Madrid, Spain</mods:affiliation></affiliation></author><author><name sortKey="Lomonossoff, George P" sort="Lomonossoff, George P" uniqKey="Lomonossoff G" first="George P." last="Lomonossoff">George P. Lomonossoff</name><affiliation><mods:affiliation>John Innes Centre, Norwich, UK</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: george.lomonossoff@bbsrc.ac.uk</mods:affiliation></affiliation><affiliation><mods:affiliation>Correspondence address: John Innes Centre, Colney lane, Norwich NR4 7UH, UK, +44‐1603‐450018</mods:affiliation></affiliation></author><author><name sortKey="Garcia, Juan A" sort="Garcia, Juan A" uniqKey="Garcia J" first="Juan A." last="García">Juan A. García</name><affiliation><mods:affiliation>Centro Nacional de Biotecnología, Campus Universidad Autónoma, Madrid, Spain</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Biotechnology Journal</title><title level="j" type="sub">Plant Biotech</title><title level="j" type="alt">BIOTECHNOLOGY JOURNAL</title><idno type="ISSN">1860-6768</idno><idno type="eISSN">1860-7314</idno><imprint><biblScope unit="vol">1</biblScope><biblScope unit="issue">10</biblScope><biblScope unit="page" from="1103">1103</biblScope><biblScope unit="page" to="1111">1111</biblScope><biblScope unit="page-count">9</biblScope><publisher>WILEY‐VCH Verlag</publisher><pubPlace>Weinheim</pubPlace><date type="published" when="2006-10">2006-10</date></imprint><idno type="ISSN">1860-6768</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1860-6768</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To extend the potential of antibodies and their derivatives to provide passive protection against enteric infections when supplied orally in crude plant extracts, we have expressed both a small immune protein (SIP) and a full‐length antibody in plants using two different plant virus vectors based on potato virus X (PVX) and cowpea mosaic virus (CPMV). The agr;SIP molecule consisted of a single chain antibody (scFv) specific for the porcine coronavirus, transmissible gastroenteritis virus (TGEV) linked to the α‐CH3 domain from human IgA. To express the full‐length IgA, the individual light and heavy chains from the TGEV‐specific mAb 6A.C3 were inserted into separate PVX constructs and plants were co‐infected with both constructs. Western blot analysis revealed the efficient expression of both the SIP and IgA molecules. Analysis of crude plant extracts revealed that both the plant‐expressed αSIP and IgA molecules could bind to and neutralize TGEV in tissue culture, indicating that active molecules were produced. Oral administration of crude extracts from antibody‐expressing plant tissue to 2‐day‐old piglets showed that both the αSIP and full‐length IgA molecules can provide in vivo protection against TGEV.</div></front></TEI><istex><corpusName>wiley</corpusName><keywords><teeft><json:string>tgev</json:string><json:string>riga</json:string><json:string>cpmv</json:string><json:string>plant extract</json:string><json:string>cowpea</json:string><json:string>mammalian cell</json:string><json:string>virus titer</json:string><json:string>clevelandii</json:string><json:string>recombinant</json:string><json:string>biotechnol</json:string><json:string>virol</json:string><json:string>kgaa</json:string><json:string>gastroenteritis</json:string><json:string>viral</json:string><json:string>seite</json:string><json:string>weinheim</json:string><json:string>positive control</json:string><json:string>clevelandii plant</json:string><json:string>verlag</json:string><json:string>gmbh</json:string><json:string>verlag gmbh</json:string><json:string>coronavirus</json:string><json:string>piglet</json:string><json:string>elisa</json:string><json:string>transgenic</json:string><json:string>encoding</json:string><json:string>plasmid</json:string><json:string>assay</json:string><json:string>negative control</json:string><json:string>western blot analysis</json:string><json:string>viral vector</json:string><json:string>sequence encoding</json:string><json:string>vivo protection</json:string><json:string>neutralization</json:string><json:string>plant tissue</json:string><json:string>high level</json:string><json:string>crude plant extract</json:string><json:string>extract</json:string><json:string>transmissible</json:string><json:string>transmissible gastroenteritis coronavirus</json:string><json:string>leader peptide</json:string><json:string>weinheim biotechnol</json:string><json:string>crude extract</json:string><json:string>biotechnology journal</json:string><json:string>riga molecule</json:string><json:string>small immune protein</json:string><json:string>transgenic plant</json:string><json:string>cowpea plant</json:string><json:string>peptide</json:string><json:string>schematic representation</json:string><json:string>newborn animal</json:string><json:string>elisa assay</json:string><json:string>tgev challenge</json:string><json:string>tissue culture</json:string><json:string>oral administration</json:string><json:string>cowpea mosaic virus</json:string><json:string>virus plaque</json:string><json:string>potato virus</json:string><json:string>transmissible gastroenteritis virus</json:string><json:string>irrelevant specificity</json:string><json:string>tgev particle</json:string><json:string>newborn pig</json:string><json:string>molecule</json:string><json:string>antibody</json:string><json:string>virus</json:string><json:string>neutralizing antibody</json:string><json:string>secondary structure</json:string><json:string>gray box</json:string><json:string>light chain</json:string><json:string>clai site</json:string><json:string>sali site</json:string><json:string>passive immunization</json:string><json:string>virus vector</json:string><json:string>agrobacterium culture</json:string><json:string>antibody expression</json:string><json:string>able dimerize</json:string><json:string>cysteine residue</json:string><json:string>second antibody</json:string><json:string>liquid nitrogen</json:string><json:string>fine powder</json:string><json:string>cell debris</json:string><json:string>present address</json:string><json:string>neutralization assay</json:string><json:string>neutralization index</json:string><json:string>day post inoculation</json:string><json:string>day post challenge</json:string><json:string>antibody molecule</json:string><json:string>tgev infection</json:string><json:string>ascitic fluid</json:string><json:string>neutralization activity</json:string><json:string>recombinant virus</json:string><json:string>marker protein</json:string><json:string>efficient dimerization</json:string><json:string>virus infection</json:string><json:string>total soluble protein</json:string><json:string>smaller size</json:string><json:string>genetic engineering</json:string><json:string>recombinant antibody</json:string><json:string>retention signal</json:string><json:string>binding activity</json:string><json:string>tgev neutralization assay</json:string><json:string>parental antibody</json:string><json:string>virus replication</json:string><json:string>expression vector</json:string></teeft></keywords><author><json:item><name>Josefa M. Alamillo</name><affiliations><json:string>Centro Nacional de Biotecnología, Campus Universidad Autónoma, Madrid, Spain</json:string><json:string>Universidad de Córdoba, Campus Rabanales, C‐6, 14071 Córdoba, Spain</json:string></affiliations></json:item><json:item><name>Wendy Monger</name><affiliations><json:string>John Innes Centre, Norwich, UK</json:string></affiliations></json:item><json:item><name>Isabel Sola</name><affiliations><json:string>Centro Nacional de Biotecnología, Campus Universidad Autónoma, Madrid, Spain</json:string></affiliations></json:item><json:item><name>Beatriz García</name><affiliations><json:string>Centro Nacional de Biotecnología, Campus Universidad Autónoma, Madrid, Spain</json:string></affiliations></json:item><json:item><name>Yolande Perrin</name><affiliations><json:string>John Innes Centre, Norwich, UK</json:string><json:string>Universitéde Technologie de Compiègne, BP 20529, 60205 Compiègne Cédex, France</json:string></affiliations></json:item><json:item><name>Marco Bestagno</name><affiliations><json:string>International Center for Genetic Engineering and Biotechnology, Trieste, Italy</json:string></affiliations></json:item><json:item><name>Oscar R. Burrone</name><affiliations><json:string>International Center for Genetic Engineering and Biotechnology, Trieste, Italy</json:string></affiliations></json:item><json:item><name>Patricia Sabella</name><affiliations><json:string>Fort Dodge Veterinaria SA, Carretera de Comprodon, Girona, Spain</json:string></affiliations></json:item><json:item><name>Joan Plana‐Durán</name><affiliations><json:string>Fort Dodge Veterinaria SA, Carretera de Comprodon, Girona, Spain</json:string></affiliations></json:item><json:item><name>Luis Enjuanes</name><affiliations><json:string>Centro Nacional de Biotecnología, Campus Universidad Autónoma, Madrid, Spain</json:string></affiliations></json:item><json:item><name>George P. Lomonossoff Dr.</name><affiliations><json:string>John Innes Centre, Norwich, UK</json:string><json:string>E-mail: george.lomonossoff@bbsrc.ac.uk</json:string><json:string>Correspondence address: John Innes Centre, Colney lane, Norwich NR4 7UH, UK, +44‐1603‐450018</json:string></affiliations></json:item><json:item><name>Juan A. García</name><affiliations><json:string>Centro Nacional de Biotecnología, Campus Universidad Autónoma, Madrid, Spain</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>IgA</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Passive immunization</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Plant virus vectors</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Small immune protein</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Transmissible gastroenteritis virus</value></json:item></subject><articleId><json:string>BIOT200600143</json:string></articleId><arkIstex>ark:/67375/WNG-4H4VNSSX-D</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>To extend the potential of antibodies and their derivatives to provide passive protection against enteric infections when supplied orally in crude plant extracts, we have expressed both a small immune protein (SIP) and a full‐length antibody in plants using two different plant virus vectors based on potato virus X (PVX) and cowpea mosaic virus (CPMV). The agr;SIP molecule consisted of a single chain antibody (scFv) specific for the porcine coronavirus, transmissible gastroenteritis virus (TGEV) linked to the α‐CH3 domain from human IgA. To express the full‐length IgA, the individual light and heavy chains from the TGEV‐specific mAb 6A.C3 were inserted into separate PVX constructs and plants were co‐infected with both constructs. Western blot analysis revealed the efficient expression of both the SIP and IgA molecules. Analysis of crude plant extracts revealed that both the plant‐expressed αSIP and IgA molecules could bind to and neutralize TGEV in tissue culture, indicating that active molecules were produced. Oral administration of crude extracts from antibody‐expressing plant tissue to 2‐day‐old piglets showed that both the αSIP and full‐length IgA molecules can provide in vivo protection against TGEV.</abstract><qualityIndicators><score>9.184</score><pdfWordCount>5774</pdfWordCount><pdfCharCount>35665</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>9</pdfPageCount><pdfPageSize>595.275 x 790.867 pts</pdfPageSize><pdfWordsPerPage>642</pdfWordsPerPage><pdfText>true</pdfText><refBibsNative>true</refBibsNative><abstractWordCount>182</abstractWordCount><abstractCharCount>1223</abstractCharCount><keywordCount>5</keywordCount></qualityIndicators><title>Use of virus vectors for the expression in plants of active full‐length and single chain anti‐coronavirus antibodies</title><pmid><json:string>17004304</json:string></pmid><genre><json:string>article</json:string></genre><host><title>Biotechnology Journal</title><language><json:string>unknown</json:string></language><doi><json:string>10.1002/(ISSN)1860-7314</json:string></doi><issn><json:string>1860-6768</json:string></issn><eissn><json:string>1860-7314</json:string></eissn><publisherId><json:string>BIOT</json:string></publisherId><volume>1</volume><issue>10</issue><pages><first>1103</first><last>1111</last><total>9</total></pages><genre><json:string>journal</json:string></genre><subject><json:item><value>Research Article</value></json:item><json:item><value>Research Articles</value></json:item></subject></host><namedEntities><unitex><date><json:string>2006</json:string></date><geogName></geogName><orgName><json:string>Spain Innes Centre, Norwich, UK</json:string><json:string>International Center for Genetic Engineering and Biotechnology</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Wendy Monger</json:string><json:string>Oscar R. Burrone</json:string><json:string>Juan A. García</json:string><json:string>George P. Lomonossoff</json:string><json:string>Isabel Sola</json:string><json:string>Marco Bestagno</json:string><json:string>Beatriz García</json:string><json:string>J. Gen</json:string><json:string>E. Dominguez</json:string><json:string>C. Supernatant</json:string><json:string>Joan Plana-Durán</json:string><json:string>Innes Centre</json:string><json:string>Luis Enjuanes</json:string><json:string>Yolande Perrin</json:string><json:string>Patricia Sabella</json:string></persName><placeName><json:string>Compiègne</json:string><json:string>Bradford</json:string><json:string>Girona</json:string><json:string>Tilburg</json:string><json:string>Hel</json:string><json:string>Trieste</json:string><json:string>Norwich</json:string><json:string>France</json:string><json:string>Italy</json:string><json:string>Spain</json:string><json:string>Netherlands</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>[4]</json:string><json:string>[12]</json:string><json:string>[6]</json:string><json:string>M. Bestagno et al.</json:string><json:string>[11]</json:string><json:string>Jimenez et al. [15]</json:string><json:string>[17]</json:string><json:string>[1]</json:string><json:string>[10]</json:string><json:string>[16]</json:string><json:string>[3]</json:string><json:string>[6, 7]</json:string><json:string>Bestagno et al.</json:string><json:string>[14]</json:string><json:string>[9]</json:string><json:string>[2]</json:string><json:string>[13]</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/WNG-4H4VNSSX-D</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - biotechnology & applied microbiology</json:string><json:string>2 - biochemical research methods</json:string></wos><scienceMetrix><json:string>1 - applied sciences</json:string><json:string>2 - enabling & strategic technologies</json:string><json:string>3 - biotechnology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Molecular Medicine</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Applied Microbiology and Biotechnology</json:string><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - General Medicine</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>4 - microbiologie</json:string></inist></categories><publicationDate>2006</publicationDate><copyrightDate>2006</copyrightDate><doi><json:string>10.1002/biot.200600143</json:string></doi><id>271923F222E75015A690589A822585977821BEC2</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-4H4VNSSX-D/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-4H4VNSSX-D/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/WNG-4H4VNSSX-D/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main">Use of virus vectors for the expression in plants of active full‐length and single chain anti‐coronavirus antibodies</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>WILEY‐VCH Verlag</publisher><pubPlace>Weinheim</pubPlace><availability><licence>Copyright © 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</licence></availability><date type="published" when="2006-10"></date></publicationStmt><notesStmt><note type="content-type" subtype="article" source="article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</note><note type="publication-type" subtype="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="article"><analytic><title level="a" type="main">Use of virus vectors for the expression in plants of active full‐length and single chain anti‐coronavirus antibodies</title><author xml:id="author-0000"><persName><forename type="first">Josefa M.</forename><surname>Alamillo</surname></persName><affiliation><orgName type="division">Centro Nacional de Biotecnología</orgName><orgName type="institution">Campus Universidad Autónoma</orgName><address><addrLine>Madrid</addrLine><addrLine>Spain</addrLine><country key="ES" xml:lang="en">SPAIN</country></address></affiliation><affiliation><orgName type="institution">Universidad de Córdoba</orgName><orgName type="institution">Campus Rabanales</orgName><address><addrLine>C‐6</addrLine><addrLine>14071 Córdoba, Spain</addrLine><country key="ES" xml:lang="en">SPAIN</country></address></affiliation></author><author xml:id="author-0001"><persName><forename type="first">Wendy</forename><surname>Monger</surname></persName><affiliation><orgName type="division">John Innes Centre</orgName><address><addrLine>Norwich</addrLine><addrLine>UK</addrLine><country key="GB" xml:lang="en">UNITED KINGDOM</country></address></affiliation></author><author xml:id="author-0002"><persName><forename type="first">Isabel</forename><surname>Sola</surname></persName><affiliation><orgName type="division">Centro Nacional de Biotecnología</orgName><orgName type="institution">Campus Universidad Autónoma</orgName><address><addrLine>Madrid</addrLine><addrLine>Spain</addrLine><country key="ES" xml:lang="en">SPAIN</country></address></affiliation></author><author xml:id="author-0003"><persName><forename type="first">Beatriz</forename><surname>García</surname></persName><affiliation><orgName type="division">Centro Nacional de Biotecnología</orgName><orgName type="institution">Campus Universidad Autónoma</orgName><address><addrLine>Madrid</addrLine><addrLine>Spain</addrLine><country key="ES" xml:lang="en">SPAIN</country></address></affiliation></author><author xml:id="author-0004"><persName><forename type="first">Yolande</forename><surname>Perrin</surname></persName><affiliation><orgName type="division">John Innes Centre</orgName><address><addrLine>Norwich</addrLine><addrLine>UK</addrLine><country key="GB" xml:lang="en">UNITED KINGDOM</country></address></affiliation><affiliation><orgName type="institution">Universitéde Technologie de Compiègne</orgName><address><addrLine>BP 20529</addrLine><addrLine>60205 Compiègne Cédex, France</addrLine><country key="FR" xml:lang="en">FRANCE</country></address></affiliation></author><author xml:id="author-0005"><persName><forename type="first">Marco</forename><surname>Bestagno</surname></persName><affiliation><orgName type="institution">International Center for Genetic Engineering and Biotechnology</orgName><address><addrLine>Trieste</addrLine><addrLine>Italy</addrLine><country key="IT" xml:lang="en">ITALY</country></address></affiliation></author><author xml:id="author-0006"><persName><forename type="first">Oscar R.</forename><surname>Burrone</surname></persName><affiliation><orgName type="institution">International Center for Genetic Engineering and Biotechnology</orgName><address><addrLine>Trieste</addrLine><addrLine>Italy</addrLine><country key="IT" xml:lang="en">ITALY</country></address></affiliation></author><author xml:id="author-0007"><persName><forename type="first">Patricia</forename><surname>Sabella</surname></persName><affiliation><address><addrLine>Fort Dodge Veterinaria SA</addrLine><addrLine>Carretera de Comprodon, Girona, Spain</addrLine><country key="ES" xml:lang="en">SPAIN</country></address></affiliation></author><author xml:id="author-0008"><persName><forename type="first">Joan</forename><surname>Plana‐Durán</surname></persName><affiliation><address><addrLine>Fort Dodge Veterinaria SA</addrLine><addrLine>Carretera de Comprodon, Girona, Spain</addrLine><country key="ES" xml:lang="en">SPAIN</country></address></affiliation></author><author xml:id="author-0009"><persName><forename type="first">Luis</forename><surname>Enjuanes</surname></persName><affiliation><orgName type="division">Centro Nacional de Biotecnología</orgName><orgName type="institution">Campus Universidad Autónoma</orgName><address><addrLine>Madrid</addrLine><addrLine>Spain</addrLine><country key="ES" xml:lang="en">SPAIN</country></address></affiliation></author><author xml:id="author-0010" role="corresp"><persName><forename type="first">George P.</forename><surname>Lomonossoff</surname><roleName type="degree">Dr.</roleName></persName><email>george.lomonossoff@bbsrc.ac.uk</email><affiliation><orgName type="division">John Innes Centre</orgName><address><addrLine>Norwich</addrLine><addrLine>UK</addrLine><country key="GB" xml:lang="en">UNITED KINGDOM</country></address></affiliation></author><author xml:id="author-0011"><persName><forename type="first">Juan A.</forename><surname>García</surname></persName><affiliation><orgName type="division">Centro Nacional de Biotecnología</orgName><orgName type="institution">Campus Universidad Autónoma</orgName><address><addrLine>Madrid</addrLine><addrLine>Spain</addrLine><country key="ES" xml:lang="en">SPAIN</country></address></affiliation></author><idno type="istex">271923F222E75015A690589A822585977821BEC2</idno><idno type="ark">ark:/67375/WNG-4H4VNSSX-D</idno><idno type="DOI">10.1002/biot.200600143</idno><idno type="unit">BIOT200600143</idno><idno type="toTypesetVersion">file:BIOT.BIOT1103.pdf</idno></analytic><monogr><title level="j" type="main">Biotechnology Journal</title><title level="j" type="sub">Plant Biotech</title><title level="j" type="alt">BIOTECHNOLOGY JOURNAL</title><idno type="pISSN">1860-6768</idno><idno type="eISSN">1860-7314</idno><idno type="book-DOI">10.1002/(ISSN)1860-7314</idno><idno type="book-part-DOI">10.1002/biot.v1:10</idno><idno type="product">BIOT</idno><imprint><biblScope unit="vol">1</biblScope><biblScope unit="issue">10</biblScope><biblScope unit="page" from="1103">1103</biblScope><biblScope unit="page" to="1111">1111</biblScope><biblScope unit="page-count">9</biblScope><publisher>WILEY‐VCH Verlag</publisher><pubPlace>Weinheim</pubPlace><date type="published" when="2006-10"></date></imprint></monogr></biblStruct></sourceDesc></fileDesc><encodingDesc><schemaRef type="ODD" url="https://xml-schema.delivery.istex.fr/tei-istex.odd"></schemaRef><appInfo><application ident="pub2tei" version="1.0.10" when="2019-12-20"><label>pub2TEI-ISTEX</label><desc>A set of style sheets for converting XML documents encoded in various scientific publisher formats into a common TEI format.
<ref target="http://www.tei-c.org/">We use TEI</ref></desc></application></appInfo></encodingDesc><profileDesc><abstract xml:lang="en" style="main"><head>Abstract</head><p>To extend the potential of antibodies and their derivatives to provide passive protection against enteric infections when supplied orally in crude plant extracts, we have expressed both a small immune protein (SIP) and a full‐length antibody in plants using two different plant virus vectors based on potato virus X (PVX) and cowpea mosaic virus (CPMV). The agr;SIP molecule consisted of a single chain antibody (scFv) specific for the porcine coronavirus, transmissible gastroenteritis virus (TGEV) linked to the α‐CH3 domain from human IgA. To express the full‐length IgA, the individual light and heavy chains from the TGEV‐specific mAb 6A.C3 were inserted into separate PVX constructs and plants were co‐infected with both constructs. Western blot analysis revealed the efficient expression of both the SIP and IgA molecules. Analysis of crude plant extracts revealed that both the plant‐expressed αSIP and IgA molecules could bind to and neutralize TGEV in tissue culture, indicating that active molecules were produced. Oral administration of crude extracts from antibody‐expressing plant tissue to 2‐day‐old piglets showed that both the αSIP and full‐length IgA molecules can provide <hi rend="italic">in vivo</hi> protection against TGEV.</p></abstract><textClass><keywords xml:lang="en"><term xml:id="kwd1">IgA</term><term xml:id="kwd2">Passive immunization</term><term xml:id="kwd3">Plant virus vectors</term><term xml:id="kwd4">Small immune protein</term><term xml:id="kwd5">Transmissible gastroenteritis virus</term></keywords><keywords rend="articleCategory"><term>Research Article</term></keywords><keywords rend="tocHeading1"><term>Research Articles</term></keywords></textClass><langUsage><language ident="en"></language></langUsage></profileDesc><revisionDesc><change when="2019-12-20" who="#istex" xml:id="pub2tei">formatting</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-4H4VNSSX-D/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>WILEY‐VCH Verlag</publisherName><publisherLoc>Weinheim</publisherLoc></publisherInfo><doi registered="yes">10.1002/(ISSN)1860-7314</doi><issn type="print">1860-6768</issn><issn type="electronic">1860-7314</issn><idGroup><id type="product" value="BIOT"></id></idGroup><titleGroup><title type="main" xml:lang="en" sort="BIOTECHNOLOGY JOURNAL">Biotechnology Journal</title><title type="subtitle">Healthcare Nutrition Technology</title><title type="short">Biotechnology Journal</title></titleGroup></publicationMeta><publicationMeta level="part" position="100"><doi origin="wiley" registered="yes">10.1002/biot.v1:10</doi><titleGroup><title type="specialIssueTitle">Plant Biotech</title></titleGroup><numberingGroup><numbering type="journalVolume" number="1">1</numbering><numbering type="journalIssue">10</numbering></numberingGroup><coverDate startDate="2006-10">October 2006</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="12" status="forIssue"><doi origin="wiley" registered="yes">10.1002/biot.200600143</doi><idGroup><id type="unit" value="BIOT200600143"></id></idGroup><countGroup><count type="pageTotal" number="9"></count></countGroup><titleGroup><title type="articleCategory">Research Article</title><title type="tocHeading1">Research Articles</title></titleGroup><copyright ownership="publisher">Copyright © 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</copyright><eventGroup><event type="manuscriptReceived" date="2006-07-28"></event><event type="manuscriptRevised" date="2006-08-15"></event><event type="manuscriptAccepted" date="2006-08-16"></event><event type="firstOnline" date="2006-09-27"></event><event type="publishedOnlineFinalForm" date="2006-10-16"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.2 mode:FullText source:HeaderRef result:HeaderRef" date="2010-03-09"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-07"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-15"></event></eventGroup><numberingGroup><numbering type="pageFirst">1103</numbering><numbering type="pageLast">1111</numbering></numberingGroup><correspondenceTo>John Innes Centre, Colney lane, Norwich NR4 7UH, UK, <b>Fax:</b> +44‐1603‐450018</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:BIOT.BIOT1103.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="4"></count><count type="tableTotal" number="0"></count><count type="referenceTotal" number="17"></count></countGroup><titleGroup><title type="main" xml:lang="en">Use of virus vectors for the expression in plants of active full‐length and single chain anti‐coronavirus antibodies</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#a1 #a5"><personName><givenNames>Josefa M.</givenNames><familyName>Alamillo</familyName></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#a2"><personName><givenNames>Wendy</givenNames><familyName>Monger</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#a1"><personName><givenNames>Isabel</givenNames><familyName>Sola</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#a1"><personName><givenNames>Beatriz</givenNames><familyName>García</familyName></personName></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#a2 #a6"><personName><givenNames>Yolande</givenNames><familyName>Perrin</familyName></personName></creator><creator xml:id="au6" creatorRole="author" affiliationRef="#a3"><personName><givenNames>Marco</givenNames><familyName>Bestagno</familyName></personName></creator><creator xml:id="au7" creatorRole="author" affiliationRef="#a3"><personName><givenNames>Oscar R.</givenNames><familyName>Burrone</familyName></personName></creator><creator xml:id="au8" creatorRole="author" affiliationRef="#a4"><personName><givenNames>Patricia</givenNames><familyName>Sabella</familyName></personName></creator><creator xml:id="au9" creatorRole="author" affiliationRef="#a4"><personName><givenNames>Joan</givenNames><familyName>Plana‐Durán</familyName></personName></creator><creator xml:id="au10" creatorRole="author" affiliationRef="#a1"><personName><givenNames>Luis</givenNames><familyName>Enjuanes</familyName></personName></creator><creator xml:id="au11" creatorRole="author" affiliationRef="#a2" corresponding="yes"><personName><givenNames>George P.</givenNames><familyName>Lomonossoff</familyName><degrees>Dr.</degrees></personName><contactDetails><email>george.lomonossoff@bbsrc.ac.uk</email></contactDetails></creator><creator xml:id="au12" creatorRole="author" affiliationRef="#a1"><personName><givenNames>Juan A.</givenNames><familyName>García</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="ES" type="organization"><unparsedAffiliation>Centro Nacional de Biotecnología, Campus Universidad Autónoma, Madrid, Spain</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="GB" type="organization"><unparsedAffiliation>John Innes Centre, Norwich, UK</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="IT" type="organization"><unparsedAffiliation>International Center for Genetic Engineering and Biotechnology, Trieste, Italy</unparsedAffiliation></affiliation><affiliation xml:id="a4" countryCode="ES" type="organization"><unparsedAffiliation>Fort Dodge Veterinaria SA, Carretera de Comprodon, Girona, Spain</unparsedAffiliation></affiliation><affiliation xml:id="a5" countryCode="ES" type="organization"><unparsedAffiliation>Universidad de Córdoba, Campus Rabanales, C‐6, 14071 Córdoba, Spain</unparsedAffiliation></affiliation><affiliation xml:id="a6" countryCode="FR" type="organization"><unparsedAffiliation>Universitéde Technologie de Compiègne, BP 20529, 60205 Compiègne Cédex, France</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">IgA</keyword><keyword xml:id="kwd2">Passive immunization</keyword><keyword xml:id="kwd3">Plant virus vectors</keyword><keyword xml:id="kwd4">Small immune protein</keyword><keyword xml:id="kwd5">Transmissible gastroenteritis virus</keyword></keywordGroup><fundingInfo><fundingAgency>EU Framework 5 Quality of Life Program Contract</fundingAgency><fundingNumber>QLK2‐CT‐2000‐00739</fundingNumber><fundingNumber>QLK2‐CT‐2002‐01050</fundingNumber></fundingInfo><fundingInfo><fundingAgency>EU FP6 “PharmaPlanta” project</fundingAgency></fundingInfo><fundingInfo><fundingAgency>Grant BIO2004‐02687 from the Spanish MEC</fundingAgency></fundingInfo><fundingInfo><fundingAgency>Grants from the Comisión interministerial de Ciencia y Tecnología (CICYT) of Spain</fundingAgency></fundingInfo><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>To extend the potential of antibodies and their derivatives to provide passive protection against enteric infections when supplied orally in crude plant extracts, we have expressed both a small immune protein (SIP) and a full‐length antibody in plants using two different plant virus vectors based on potato virus X (PVX) and cowpea mosaic virus (CPMV). The agr;SIP molecule consisted of a single chain antibody (scFv) specific for the porcine coronavirus, transmissible gastroenteritis virus (TGEV) linked to the α‐CH3 domain from human IgA. To express the full‐length IgA, the individual light and heavy chains from the TGEV‐specific mAb 6A.C3 were inserted into separate PVX constructs and plants were co‐infected with both constructs. Western blot analysis revealed the efficient expression of both the SIP and IgA molecules. Analysis of crude plant extracts revealed that both the plant‐expressed αSIP and IgA molecules could bind to and neutralize TGEV in tissue culture, indicating that active molecules were produced. Oral administration of crude extracts from antibody‐expressing plant tissue to 2‐day‐old piglets showed that both the αSIP and full‐length IgA molecules can provide <i>in vivo</i> protection against TGEV.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Use of virus vectors for the expression in plants of active full‐length and single chain anti‐coronavirus antibodies</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Use of virus vectors for the expression in plants of active full‐length and single chain anti‐coronavirus antibodies</title></titleInfo><name type="personal"><namePart type="given">Josefa M.</namePart><namePart type="family">Alamillo</namePart><affiliation>Centro Nacional de Biotecnología, Campus Universidad Autónoma, Madrid, Spain</affiliation><affiliation>Universidad de Córdoba, Campus Rabanales, C‐6, 14071 Córdoba, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Wendy</namePart><namePart type="family">Monger</namePart><affiliation>John Innes Centre, Norwich, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Isabel</namePart><namePart type="family">Sola</namePart><affiliation>Centro Nacional de Biotecnología, Campus Universidad Autónoma, Madrid, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Beatriz</namePart><namePart type="family">García</namePart><affiliation>Centro Nacional de Biotecnología, Campus Universidad Autónoma, Madrid, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yolande</namePart><namePart type="family">Perrin</namePart><affiliation>John Innes Centre, Norwich, UK</affiliation><affiliation>Universitéde Technologie de Compiègne, BP 20529, 60205 Compiègne Cédex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Marco</namePart><namePart type="family">Bestagno</namePart><affiliation>International Center for Genetic Engineering and Biotechnology, Trieste, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Oscar R.</namePart><namePart type="family">Burrone</namePart><affiliation>International Center for Genetic Engineering and Biotechnology, Trieste, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Patricia</namePart><namePart type="family">Sabella</namePart><affiliation>Fort Dodge Veterinaria SA, Carretera de Comprodon, Girona, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Joan</namePart><namePart type="family">Plana‐Durán</namePart><affiliation>Fort Dodge Veterinaria SA, Carretera de Comprodon, Girona, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Luis</namePart><namePart type="family">Enjuanes</namePart><affiliation>Centro Nacional de Biotecnología, Campus Universidad Autónoma, Madrid, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">George P.</namePart><namePart type="family">Lomonossoff</namePart><namePart type="termsOfAddress">Dr.</namePart><affiliation>John Innes Centre, Norwich, UK</affiliation><affiliation>E-mail: george.lomonossoff@bbsrc.ac.uk</affiliation><affiliation>Correspondence address: John Innes Centre, Colney lane, Norwich NR4 7UH, UK, +44‐1603‐450018</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Juan A.</namePart><namePart type="family">García</namePart><affiliation>Centro Nacional de Biotecnología, Campus Universidad Autónoma, Madrid, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>WILEY‐VCH Verlag</publisher><place><placeTerm type="text">Weinheim</placeTerm></place><dateIssued encoding="w3cdtf">2006-10</dateIssued><dateCaptured encoding="w3cdtf">2006-07-28</dateCaptured><dateValid encoding="w3cdtf">2006-08-16</dateValid><copyrightDate encoding="w3cdtf">2006</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="figures">4</extent><extent unit="tables">0</extent><extent unit="references">17</extent></physicalDescription><abstract lang="en">To extend the potential of antibodies and their derivatives to provide passive protection against enteric infections when supplied orally in crude plant extracts, we have expressed both a small immune protein (SIP) and a full‐length antibody in plants using two different plant virus vectors based on potato virus X (PVX) and cowpea mosaic virus (CPMV). The agr;SIP molecule consisted of a single chain antibody (scFv) specific for the porcine coronavirus, transmissible gastroenteritis virus (TGEV) linked to the α‐CH3 domain from human IgA. To express the full‐length IgA, the individual light and heavy chains from the TGEV‐specific mAb 6A.C3 were inserted into separate PVX constructs and plants were co‐infected with both constructs. Western blot analysis revealed the efficient expression of both the SIP and IgA molecules. Analysis of crude plant extracts revealed that both the plant‐expressed αSIP and IgA molecules could bind to and neutralize TGEV in tissue culture, indicating that active molecules were produced. Oral administration of crude extracts from antibody‐expressing plant tissue to 2‐day‐old piglets showed that both the αSIP and full‐length IgA molecules can provide in vivo protection against TGEV.</abstract><note type="funding">EU Framework 5 Quality of Life Program Contract - No. QLK2‐CT‐2000‐00739; No. QLK2‐CT‐2002‐01050; </note><note type="funding">EU FP6 “PharmaPlanta” project</note><note type="funding">Grant BIO2004‐02687 from the Spanish MEC</note><note type="funding">Grants from the Comisión interministerial de Ciencia y Tecnología (CICYT) of Spain</note><subject lang="en"><genre>keywords</genre><topic>IgA</topic><topic>Passive immunization</topic><topic>Plant virus vectors</topic><topic>Small immune protein</topic><topic>Transmissible gastroenteritis virus</topic></subject><relatedItem type="host"><titleInfo><title>Biotechnology Journal</title><subTitle>Healthcare Nutrition Technology</subTitle></titleInfo><titleInfo type="abbreviated"><title>Biotechnology Journal</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><subject><genre>article-category</genre><topic>Research Article</topic><topic>Research Articles</topic></subject><identifier type="ISSN">1860-6768</identifier><identifier type="eISSN">1860-7314</identifier><identifier type="DOI">10.1002/(ISSN)1860-7314</identifier><identifier type="PublisherID">BIOT</identifier><part><date>2006</date><detail type="title"><title>Plant Biotech</title></detail><detail type="volume"><caption>vol.</caption><number>1</number></detail><detail type="issue"><caption>no.</caption><number>10</number></detail><extent unit="pages"><start>1103</start><end>1111</end><total>9</total></extent></part></relatedItem><relatedItem type="references" displayLabel="cit1"><titleInfo><title>Enjuanes, L., van der Zeijst, B. A. M., Molecular basis of TGE coronavirus epidemiology. In: Siddell, S. G. (Ed.), The Coronaviridae, Plenum Press, New York 1995, pp. 337‐376.</title></titleInfo><genre>other</genre></relatedItem><relatedItem type="references" displayLabel="cit2"><titleInfo><title>Residues involved in the formation of the antigenic sites of the S protein of transmissible gastroenteritis coronavirus.</title></titleInfo><name type="personal"><namePart type="given">F.</namePart><namePart type="family">Gebauer</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">W. A. P.</namePart><namePart type="family">Posthumus</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">I.</namePart><namePart type="family">Correa</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Suñé</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Gebauer, F., Posthumus, W. A. P., Correa, I., Suñé, C. et al., Residues involved in the formation of the antigenic sites of the S protein of transmissible gastroenteritis coronavirus. Virology 1991 183, 225–238.</note><part><date>1991</date><detail type="volume"><caption>vol.</caption><number>183</number></detail><extent unit="pages"><start>225</start><end>238</end></extent></part><relatedItem type="host"><titleInfo><title>Virology</title></titleInfo><part><date>1991</date><detail type="volume"><caption>vol.</caption><number>183</number></detail><extent unit="pages"><start>225</start><end>238</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit3"><titleInfo><title>Interference of coronavirus infection by expression of immunoglobulin G (IgG) or IgA virus‐neutralizing antibodies.</title></titleInfo><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Castilla</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">I.</namePart><namePart type="family">Sola</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L.</namePart><namePart type="family">Enjuanes</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Castilla, J., Sola, I., Enjuanes, L., Interference of coronavirus infection by expression of immunoglobulin G (IgG) or IgA virus‐neutralizing antibodies. J. Virol. 1997 71, 5251–5258.</note><part><date>1997</date><detail type="volume"><caption>vol.</caption><number>71</number></detail><extent unit="pages"><start>5251</start><end>5258</end></extent></part><relatedItem type="host"><titleInfo><title>J. Virol.</title></titleInfo><part><date>1997</date><detail type="volume"><caption>vol.</caption><number>71</number></detail><extent unit="pages"><start>5251</start><end>5258</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit4"><titleInfo><title>Transgenic mice secreting coronavirus neutralizing antibodies into the milk.</title></titleInfo><name type="personal"><namePart type="given">I.</namePart><namePart type="family">Sola</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Castilla</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B.</namePart><namePart type="family">Pintado</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. M.</namePart><namePart type="family">Sánchez‐Morgado</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Sola, I., Castilla, J., Pintado, B., Sánchez‐Morgado, J. M. et al., Transgenic mice secreting coronavirus neutralizing antibodies into the milk. J. Virol. 1998 72, 3762–3771.</note><part><date>1998</date><detail type="volume"><caption>vol.</caption><number>72</number></detail><extent unit="pages"><start>3762</start><end>3771</end></extent></part><relatedItem type="host"><titleInfo><title>J. Virol.</title></titleInfo><part><date>1998</date><detail type="volume"><caption>vol.</caption><number>72</number></detail><extent unit="pages"><start>3762</start><end>3771</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit5"><titleInfo><title>Mammalian cell expression of dimeric small immune proteins (SIP).</title></titleInfo><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Li</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Pedraza</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Bestagno</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Mancardi</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Li, E., Pedraza, A., Bestagno, M., Mancardi, S. et al., Mammalian cell expression of dimeric small immune proteins (SIP). Protein Eng. 1997 10, 731–736.</note><part><date>1997</date><detail type="volume"><caption>vol.</caption><number>10</number></detail><extent unit="pages"><start>731</start><end>736</end></extent></part><relatedItem type="host"><titleInfo><title>Protein Eng.</title></titleInfo><part><date>1997</date><detail type="volume"><caption>vol.</caption><number>10</number></detail><extent unit="pages"><start>731</start><end>736</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit6"><titleInfo><title>Plantibodies: application, advantages and bottlenecks.</title></titleInfo><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Stoger</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Sack</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R.</namePart><namePart type="family">Fischer</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Christou</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Stoger, E., Sack, M., Fischer, R., Christou, P., Plantibodies: application, advantages and bottlenecks. Curr. Opin. Biotechnol. 2002 13, 161–166.</note><part><date>2002</date><detail type="volume"><caption>vol.</caption><number>13</number></detail><extent unit="pages"><start>161</start><end>166</end></extent></part><relatedItem type="host"><titleInfo><title>Curr. Opin. Biotechnol.</title></titleInfo><part><date>2002</date><detail type="volume"><caption>vol.</caption><number>13</number></detail><extent unit="pages"><start>161</start><end>166</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit7"><titleInfo><title>Plant‐based production of biopharmaceuticals.</title></titleInfo><name type="personal"><namePart type="given">R.</namePart><namePart type="family">Fischer</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Stoger</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Schillberg</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Christou</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R. M.</namePart><namePart type="family">Twyman</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Fischer, R., Stoger, E., Schillberg, S., Christou, P., Twyman, R. M., Plant‐based production of biopharmaceuticals. Curr. Opin. Plant Biol. 2004 7, 152–158.</note><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>7</number></detail><extent unit="pages"><start>152</start><end>158</end></extent></part><relatedItem type="host"><titleInfo><title>Curr. Opin. Plant Biol.</title></titleInfo><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>7</number></detail><extent unit="pages"><start>152</start><end>158</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit8"><titleInfo><title>An antibody derivative expressed from viral vectors passively immunizes pigs against transmissible gastroenteritis virus when supplied orally in crude plant extracts.</title></titleInfo><name type="personal"><namePart type="given">W.</namePart><namePart type="family">Monger</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. M.</namePart><namePart type="family">Alamillo</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">I.</namePart><namePart type="family">Sola</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y.</namePart><namePart type="family">Perrin</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Monger, W., Alamillo, J. M., Sola, I., Perrin, Y. et al., An antibody derivative expressed from viral vectors passively immunizes pigs against transmissible gastroenteritis virus when supplied orally in crude plant extracts. Plant Biotechnol. J. 2006, DOI: 10.1111/j.1467‐7652.2006.00206.x</note><part><date>2006</date></part><relatedItem type="host"><titleInfo><title>Plant Biotechnol. J.</title></titleInfo><part><date>2006</date></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit9"><titleInfo><title>RNADNA interactions and DNA methylation in post‐transcriptional gene silencing.</title></titleInfo><name type="personal"><namePart type="given">L.</namePart><namePart type="family">Jones</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A. J.</namePart><namePart type="family">Hamilton</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">O.</namePart><namePart type="family">Voinnet</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C. L.</namePart><namePart type="family">Thomas</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Jones, L., Hamilton, A. J., Voinnet, O., Thomas, C. L. et al., RNADNA interactions and DNA methylation in post‐transcriptional gene silencing. Plant Cell 1999 11, 2291–2302.</note><part><date>1999</date><detail type="volume"><caption>vol.</caption><number>11</number></detail><extent unit="pages"><start>2291</start><end>2302</end></extent></part><relatedItem type="host"><titleInfo><title>Plant Cell</title></titleInfo><part><date>1999</date><detail type="volume"><caption>vol.</caption><number>11</number></detail><extent unit="pages"><start>2291</start><end>2302</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit10"><titleInfo><title>Cowpea mosaic virus‐based systems for the production of antigens and antibodies in plants.</title></titleInfo><name type="personal"><namePart type="given">L.</namePart><namePart type="family">Liu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M. C.</namePart><namePart type="family">Cañizares</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">W.</namePart><namePart type="family">Monger</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y.</namePart><namePart type="family">Perrin</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Liu, L., Cañizares, M. C., Monger, W., Perrin, Y. et al., Cowpea mosaic virus‐based systems for the production of antigens and antibodies in plants. Vaccine 2005 23, 1788–1792.</note><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>23</number></detail><extent unit="pages"><start>1788</start><end>1792</end></extent></part><relatedItem type="host"><titleInfo><title>Vaccine</title></titleInfo><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>23</number></detail><extent unit="pages"><start>1788</start><end>1792</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit11"><titleInfo><title>Agroinfection as a rapid method for propagating cowpea mosaic virus‐based constructs.</title></titleInfo><name type="personal"><namePart type="given">L.</namePart><namePart type="family">Liu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G. P.</namePart><namePart type="family">Lomonossoff</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Liu, L., Lomonossoff, G. P., Agroinfection as a rapid method for propagating cowpea mosaic virus‐based constructs. J. Virol. Methods 2002 105, 343–348.</note><part><date>2002</date><detail type="volume"><caption>vol.</caption><number>105</number></detail><extent unit="pages"><start>343</start><end>348</end></extent></part><relatedItem type="host"><titleInfo><title>J. Virol. Methods</title></titleInfo><part><date>2002</date><detail type="volume"><caption>vol.</caption><number>105</number></detail><extent unit="pages"><start>343</start><end>348</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit12"><titleInfo><title>The use of viral vectors to produce hepatitis B virus core particles in plants.</title></titleInfo><name type="personal"><namePart type="given">I. A.</namePart><namePart type="family">Mechtcheriakova</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M. A.</namePart><namePart type="family">Eldarov</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L.</namePart><namePart type="family">Nicholson</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Shanks</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Mechtcheriakova, I. A., Eldarov, M. A., Nicholson, L., Shanks, M. et al., The use of viral vectors to produce hepatitis B virus core particles in plants. J. Virol. Methods 2006 131, 10–15.</note><part><date>2006</date><detail type="volume"><caption>vol.</caption><number>131</number></detail><extent unit="pages"><start>10</start><end>15</end></extent></part><relatedItem type="host"><titleInfo><title>J. Virol. Methods</title></titleInfo><part><date>2006</date><detail type="volume"><caption>vol.</caption><number>131</number></detail><extent unit="pages"><start>10</start><end>15</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit13"><titleInfo><title>Antigenic structure of the E2 glycoprotein from transmissible gastroenteritis coronavirus.</title></titleInfo><name type="personal"><namePart type="given">I.</namePart><namePart type="family">Correa</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Jiménez</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Suñé</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M. J.</namePart><namePart type="family">Bullido</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L.</namePart><namePart type="family">Enjuanes</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Correa, I., Jiménez, G., Suñé, C., Bullido, M. J., Enjuanes, L., Antigenic structure of the E2 glycoprotein from transmissible gastroenteritis coronavirus. Virus Res. 1988 10, 77–93.</note><part><date>1988</date><detail type="volume"><caption>vol.</caption><number>10</number></detail><extent unit="pages"><start>77</start><end>93</end></extent></part><relatedItem type="host"><titleInfo><title>Virus Res.</title></titleInfo><part><date>1988</date><detail type="volume"><caption>vol.</caption><number>10</number></detail><extent unit="pages"><start>77</start><end>93</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit14"><titleInfo><title>Targeted recombination demonstrates that the spike gene of transmissible gastroenteritis coronavirus is a determinant of its enteric tropism and virulence.</title></titleInfo><name type="personal"><namePart type="given">C. M.</namePart><namePart type="family">Sánchez</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Izeta</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. M.</namePart><namePart type="family">Sánchez‐Morgado</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Alonso</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Sánchez, C. M., Izeta, A., Sánchez‐Morgado, J. M., Alonso, S. et al., Targeted recombination demonstrates that the spike gene of transmissible gastroenteritis coronavirus is a determinant of its enteric tropism and virulence. J. Virol. 1999 73, 7607–7618.</note><part><date>1999</date><detail type="volume"><caption>vol.</caption><number>73</number></detail><extent unit="pages"><start>7607</start><end>7618</end></extent></part><relatedItem type="host"><titleInfo><title>J. Virol.</title></titleInfo><part><date>1999</date><detail type="volume"><caption>vol.</caption><number>73</number></detail><extent unit="pages"><start>7607</start><end>7618</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit15"><titleInfo><title>Critical epitopes in transmissible gastroenteritis virus neutralization.</title></titleInfo><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Jiménez</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">I.</namePart><namePart type="family">Correa</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M. P.</namePart><namePart type="family">Melgosa</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M. J.</namePart><namePart type="family">Bullido</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L.</namePart><namePart type="family">Enjuanes</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Jiménez, G., Correa, I., Melgosa, M. P., Bullido, M. J., Enjuanes, L., Critical epitopes in transmissible gastroenteritis virus neutralization. J. Virol. 1986 60, 131–139.</note><part><date>1986</date><detail type="volume"><caption>vol.</caption><number>60</number></detail><extent unit="pages"><start>131</start><end>139</end></extent></part><relatedItem type="host"><titleInfo><title>J. Virol.</title></titleInfo><part><date>1986</date><detail type="volume"><caption>vol.</caption><number>60</number></detail><extent unit="pages"><start>131</start><end>139</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit16"><titleInfo><title>Expression and assembly of a full‐length monoclonal antibody in plants using a plant virus vector.</title></titleInfo><name type="personal"><namePart type="given">T.</namePart><namePart type="family">Verch</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">V.</namePart><namePart type="family">Yusibov</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H.</namePart><namePart type="family">Koprowski</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Verch, T., Yusibov, V., Koprowski, H., Expression and assembly of a full‐length monoclonal antibody in plants using a plant virus vector. J. Immunol. Methods 1998 230, 69–75.</note><part><date>1998</date><detail type="volume"><caption>vol.</caption><number>230</number></detail><extent unit="pages"><start>69</start><end>75</end></extent></part><relatedItem type="host"><titleInfo><title>J. Immunol. Methods</title></titleInfo><part><date>1998</date><detail type="volume"><caption>vol.</caption><number>230</number></detail><extent unit="pages"><start>69</start><end>75</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit17"><titleInfo><title>Selective targeting of tumoral vasculature: comparison of different formats of an antibody (L19) to the ED‐B domain of fibronectin.</title></titleInfo><name type="personal"><namePart type="given">L.</namePart><namePart type="family">Borsi</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Balza</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Bestagno</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Castellani</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Borsi, L., Balza, E., Bestagno, M., Castellani, P. et al., Selective targeting of tumoral vasculature: comparison of different formats of an antibody (L19) to the ED‐B domain of fibronectin. Int. J. Cancer 2002 102, 75–85.</note><part><date>2002</date><detail type="volume"><caption>vol.</caption><number>102</number></detail><extent unit="pages"><start>75</start><end>85</end></extent></part><relatedItem type="host"><titleInfo><title>Int. J. Cancer</title></titleInfo><part><date>2002</date><detail type="volume"><caption>vol.</caption><number>102</number></detail><extent unit="pages"><start>75</start><end>85</end></extent></part></relatedItem></relatedItem><identifier type="istex">271923F222E75015A690589A822585977821BEC2</identifier><identifier type="ark">ark:/67375/WNG-4H4VNSSX-D</identifier><identifier type="DOI">10.1002/biot.200600143</identifier><identifier type="ArticleID">BIOT200600143</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-L0C46X92-X">wiley</recordContentSource><recordOrigin>Converted from (version ) to MODS version 3.6.</recordOrigin><recordCreationDate encoding="w3cdtf">2019-11-13</recordCreationDate></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-4H4VNSSX-D/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001888 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 001888 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:271923F222E75015A690589A822585977821BEC2
|texte= Use of virus vectors for the expression in plants of active full‐length and single chain anti‐coronavirus antibodies
}}
| This area was generated with Dilib version V0.6.33. |  |