Outbreaks of human coronavirus in a paediatric and neonatal intensive care unit
Identifieur interne : 001010 ( Istex/Corpus ); précédent : 001009; suivant : 001011Outbreaks of human coronavirus in a paediatric and neonatal intensive care unit
Auteurs : Arnaud Gagneur ; Sophie Vallet ; Pierre J. Talbot ; Marie-Christine Legrand-Quillien ; Bertrand Picard ; Christopher Payan ; Jacques SizunSource :
- European Journal of Pediatrics [ 0340-6199 ] ; 2008-12-01.
English descriptors
- KwdEn :
Abstract
Abstract: Human coronavirus 229E (HCoV) has been recently recognized as a potential agent of nosocomial viral respiratory infections (NRVI) in high-risk infants. We have confirmed this as fact through the study of a 1-year period of HCoV outbreaks occurring during a prospective survey of NRVI in a paediatric and neonatal intensive care unit (PNICU) using new molecular techniques for HCoV detection. Nasal samples obtained at admission and weekly thereafter for all hospitalised children, as well as monthly nasal samples from staff, were analysed using immunofluorescence for respiratory syncitial virus (RSV), influenza viruses A and B, paramyxoviruses 1, 2, 3 and adenoviruses. RT-PCR was used for HCoV detection. During the year 1998, 43 HCoV-related NRVI were detected in 152 neonates (incidence 28.3%), and 7 HCoV-related NRVI were found in 92 children (incidence 7.6%). Three HCoV-related outbreaks were observed (February, August and December), associated with a high prevalence of HCoV infection in the staff. During the August outbreak, 50% to 78% of hospitalised neonates and children were infected. Seventy-five percent of hospitalised preterm neonates with a gestational age less than 32 weeks and 52.4% of staff members were infected. Risk factors for NRVI in neonates were birth weight, gestational age, ventilation, oxygenation and hospitalisation length. Ninety-two percent of infected preterm neonates were symptomatic, mainly with bradycardia and respiratory worsening. These data provide additional evidence for a possibly significant role of HCoV in NRVI in a PNICU. The role of staff or hospitalised children in spreading HCoV is hypothesised.
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DOI: 10.1007/s00431-008-0687-0
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We have confirmed this as fact through the study of a 1-year period of HCoV outbreaks occurring during a prospective survey of NRVI in a paediatric and neonatal intensive care unit (PNICU) using new molecular techniques for HCoV detection. Nasal samples obtained at admission and weekly thereafter for all hospitalised children, as well as monthly nasal samples from staff, were analysed using immunofluorescence for respiratory syncitial virus (RSV), influenza viruses A and B, paramyxoviruses 1, 2, 3 and adenoviruses. RT-PCR was used for HCoV detection. During the year 1998, 43 HCoV-related NRVI were detected in 152 neonates (incidence 28.3%), and 7 HCoV-related NRVI were found in 92 children (incidence 7.6%). Three HCoV-related outbreaks were observed (February, August and December), associated with a high prevalence of HCoV infection in the staff. During the August outbreak, 50% to 78% of hospitalised neonates and children were infected. Seventy-five percent of hospitalised preterm neonates with a gestational age less than 32 weeks and 52.4% of staff members were infected. Risk factors for NRVI in neonates were birth weight, gestational age, ventilation, oxygenation and hospitalisation length. Ninety-two percent of infected preterm neonates were symptomatic, mainly with bradycardia and respiratory worsening. These data provide additional evidence for a possibly significant role of HCoV in NRVI in a PNICU. 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We have confirmed this as fact through the study of a 1-year period of HCoV outbreaks occurring during a prospective survey of NRVI in a paediatric and neonatal intensive care unit (PNICU) using new molecular techniques for HCoV detection. Nasal samples obtained at admission and weekly thereafter for all hospitalised children, as well as monthly nasal samples from staff, were analysed using immunofluorescence for respiratory syncitial virus (RSV), influenza viruses A and B, paramyxoviruses 1, 2, 3 and adenoviruses. RT-PCR was used for HCoV detection. During the year 1998, 43 HCoV-related NRVI were detected in 152 neonates (incidence 28.3%), and 7 HCoV-related NRVI were found in 92 children (incidence 7.6%). Three HCoV-related outbreaks were observed (February, August and December), associated with a high prevalence of HCoV infection in the staff. During the August outbreak, 50% to 78% of hospitalised neonates and children were infected. Seventy-five percent of hospitalised preterm neonates with a gestational age less than 32 weeks and 52.4% of staff members were infected. Risk factors for NRVI in neonates were birth weight, gestational age, ventilation, oxygenation and hospitalisation length. Ninety-two percent of infected preterm neonates were symptomatic, mainly with bradycardia and respiratory worsening. These data provide additional evidence for a possibly significant role of HCoV in NRVI in a PNICU. 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Seventy-five percent of hospitalised preterm neonates with a gestational age less than 32 weeks and 52.4% of staff members were infected. Risk factors for NRVI in neonates were birth weight, gestational age, ventilation, oxygenation and hospitalisation length. Ninety-two percent of infected preterm neonates were symptomatic, mainly with bradycardia and respiratory worsening. These data provide additional evidence for a possibly significant role of HCoV in NRVI in a PNICU. 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Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1 Aff4" CorrespondingAffiliationID="Aff4"><AuthorName DisplayOrder="Western"><GivenName>Arnaud</GivenName><FamilyName>Gagneur</FamilyName></AuthorName><Contact><Phone>+33-2-98223309</Phone><Fax>+33-2-98223619</Fax><Email>arnaud.gagneur@chu-brest.fr</Email></Contact></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Sophie</GivenName><FamilyName>Vallet</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff3"><AuthorName DisplayOrder="Western"><GivenName>Pierre</GivenName><GivenName>J.</GivenName><FamilyName>Talbot</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Marie-Christine</GivenName><FamilyName>Legrand-Quillien</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Bertrand</GivenName><FamilyName>Picard</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Christopher</GivenName><FamilyName>Payan</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Jacques</GivenName><FamilyName>Sizun</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgDivision>Department of Paediatrics, EA 3882 Laboratory of Biodiversity and Microbial Ecology</OrgDivision><OrgName>University Hospital</OrgName><OrgAddress><City>Brest</City><Country Code="FR">France</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Department of Microbiology, EA 3882 Laboratory of Biodiversity and Microbial Ecology</OrgDivision><OrgName>University Hospital</OrgName><OrgAddress><City>Brest</City><Country Code="FR">France</Country></OrgAddress></Affiliation><Affiliation ID="Aff3"><OrgDivision>Laboratory of Neuroimmunovirology, INRS-Institut Armand-Frappier</OrgDivision><OrgName>University of Quebec</OrgName><OrgAddress><City>Laval</City><State>Quebec</State><Country Code="CA">Canada</Country></OrgAddress></Affiliation><Affiliation ID="Aff4"><OrgName>Unité de Réanimation Pédiatrique et Néonatalogie, Département de Pédiatrie CHU</OrgName><OrgAddress><Postcode>29609</Postcode><City>Brest</City><Country Code="FR">France</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><Para>Human coronavirus 229E (HCoV) has been recently recognized as a potential agent of nosocomial viral respiratory infections (NRVI) in high-risk infants. We have confirmed this as fact through the study of a 1-year period of HCoV outbreaks occurring during a prospective survey of NRVI in a paediatric and neonatal intensive care unit (PNICU) using new molecular techniques for HCoV detection. Nasal samples obtained at admission and weekly thereafter for all hospitalised children, as well as monthly nasal samples from staff, were analysed using immunofluorescence for respiratory syncitial virus (RSV), influenza viruses A and B, paramyxoviruses 1, 2, 3 and adenoviruses. RT-PCR was used for HCoV detection. During the year 1998, 43 HCoV-related NRVI were detected in 152 neonates (incidence 28.3%), and 7 HCoV-related NRVI were found in 92 children (incidence 7.6%). Three HCoV-related outbreaks were observed (February, August and December), associated with a high prevalence of HCoV infection in the staff. During the August outbreak, 50% to 78% of hospitalised neonates and children were infected. Seventy-five percent of hospitalised preterm neonates with a gestational age less than 32 weeks and 52.4% of staff members were infected. Risk factors for NRVI in neonates were birth weight, gestational age, ventilation, oxygenation and hospitalisation length. Ninety-two percent of infected preterm neonates were symptomatic, mainly with bradycardia and respiratory worsening. These data provide additional evidence for a possibly significant role of HCoV in NRVI in a PNICU. The role of staff or hospitalised children in spreading HCoV is hypothesised.</Para></Abstract><KeywordGroup Language="En"><Heading>Keywords</Heading><Keyword>Human coronavirus</Keyword><Keyword>Outbreak</Keyword><Keyword>Neonates</Keyword><Keyword>Nosocomial infection</Keyword><Keyword>PNICU</Keyword></KeywordGroup></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Outbreaks of human coronavirus in a paediatric and neonatal intensive care unit</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Outbreaks of human coronavirus in a paediatric and neonatal intensive care unit</title></titleInfo><name type="personal" displayLabel="corresp"><namePart type="given">Arnaud</namePart><namePart type="family">Gagneur</namePart><affiliation>Department of Paediatrics, EA 3882 Laboratory of Biodiversity and Microbial Ecology, University Hospital, Brest, France</affiliation><affiliation>Unité de Réanimation Pédiatrique et Néonatalogie, Département de Pédiatrie CHU, 29609, Brest, France</affiliation><affiliation>E-mail: arnaud.gagneur@chu-brest.fr</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sophie</namePart><namePart type="family">Vallet</namePart><affiliation>Department of Microbiology, EA 3882 Laboratory of Biodiversity and Microbial Ecology, University Hospital, Brest, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Pierre</namePart><namePart type="given">J.</namePart><namePart type="family">Talbot</namePart><affiliation>Laboratory of Neuroimmunovirology, INRS-Institut Armand-Frappier, University of Quebec, Laval, Quebec, Canada</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Marie-Christine</namePart><namePart type="family">Legrand-Quillien</namePart><affiliation>Department of Microbiology, EA 3882 Laboratory of Biodiversity and Microbial Ecology, University Hospital, Brest, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Bertrand</namePart><namePart type="family">Picard</namePart><affiliation>Department of Microbiology, EA 3882 Laboratory of Biodiversity and Microbial Ecology, University Hospital, Brest, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Christopher</namePart><namePart type="family">Payan</namePart><affiliation>Department of Microbiology, EA 3882 Laboratory of Biodiversity and Microbial Ecology, University Hospital, Brest, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jacques</namePart><namePart type="family">Sizun</namePart><affiliation>Department of Paediatrics, EA 3882 Laboratory of Biodiversity and Microbial Ecology, University Hospital, Brest, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer-Verlag</publisher><place><placeTerm type="text">Berlin/Heidelberg</placeTerm></place><dateCreated encoding="w3cdtf">2007-10-19</dateCreated><dateIssued encoding="w3cdtf">2008-12-01</dateIssued><copyrightDate encoding="w3cdtf">2008</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: Human coronavirus 229E (HCoV) has been recently recognized as a potential agent of nosocomial viral respiratory infections (NRVI) in high-risk infants. We have confirmed this as fact through the study of a 1-year period of HCoV outbreaks occurring during a prospective survey of NRVI in a paediatric and neonatal intensive care unit (PNICU) using new molecular techniques for HCoV detection. Nasal samples obtained at admission and weekly thereafter for all hospitalised children, as well as monthly nasal samples from staff, were analysed using immunofluorescence for respiratory syncitial virus (RSV), influenza viruses A and B, paramyxoviruses 1, 2, 3 and adenoviruses. RT-PCR was used for HCoV detection. During the year 1998, 43 HCoV-related NRVI were detected in 152 neonates (incidence 28.3%), and 7 HCoV-related NRVI were found in 92 children (incidence 7.6%). Three HCoV-related outbreaks were observed (February, August and December), associated with a high prevalence of HCoV infection in the staff. During the August outbreak, 50% to 78% of hospitalised neonates and children were infected. Seventy-five percent of hospitalised preterm neonates with a gestational age less than 32 weeks and 52.4% of staff members were infected. Risk factors for NRVI in neonates were birth weight, gestational age, ventilation, oxygenation and hospitalisation length. Ninety-two percent of infected preterm neonates were symptomatic, mainly with bradycardia and respiratory worsening. These data provide additional evidence for a possibly significant role of HCoV in NRVI in a PNICU. The role of staff or hospitalised children in spreading HCoV is hypothesised.</abstract><note>Original Paper</note><subject lang="en"><genre>Keywords</genre><topic>Human coronavirus</topic><topic>Outbreak</topic><topic>Neonates</topic><topic>Nosocomial infection</topic><topic>PNICU</topic></subject><relatedItem type="host"><titleInfo><title>European Journal of Pediatrics</title></titleInfo><titleInfo type="abbreviated"><title>Eur J Pediatr</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2008-10-19</dateIssued><copyrightDate encoding="w3cdtf">2008</copyrightDate></originInfo><subject><genre>Medicine & Public Health</genre><topic>Pediatrics</topic></subject><identifier type="ISSN">0340-6199</identifier><identifier type="eISSN">1432-1076</identifier><identifier type="JournalID">431</identifier><identifier type="IssueArticleCount">22</identifier><identifier type="VolumeIssueCount">12</identifier><part><date>2008</date><detail type="volume"><number>167</number><caption>vol.</caption></detail><detail type="issue"><number>12</number><caption>no.</caption></detail><extent unit="pages"><start>1427</start><end>1434</end></extent></part><recordInfo><recordOrigin>Springer-Verlag, 2008</recordOrigin></recordInfo></relatedItem><identifier type="istex">9EB19D0F976F6EE91B0D932C06F1D4F86693082A</identifier><identifier type="ark">ark:/67375/VQC-RGR0DVDB-N</identifier><identifier type="DOI">10.1007/s00431-008-0687-0</identifier><identifier type="ArticleID">687</identifier><identifier type="ArticleID">s00431-008-0687-0</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer-Verlag, 2008</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Springer-Verlag, 2008</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-RGR0DVDB-N/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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