Evaluation of antibody responses against SARS coronaviral nucleocapsid or spike proteins by immunoblotting or ELISA
Identifieur interne : 000178 ( Istex/Corpus ); précédent : 000177; suivant : 000179Evaluation of antibody responses against SARS coronaviral nucleocapsid or spike proteins by immunoblotting or ELISA
Auteurs : Li-Rung Huang ; Chi-Ming Chiu ; Shiou-Hwei Yeh ; Wen-Hung Huang ; Po-Ren Hsueh ; Wen-Zieh Yang ; Jyh-Yuan Yang ; Ih-Jen Su ; Shan-Chwen Chang ; Pei-Jer ChenSource :
- Journal of Medical Virology [ 0146-6615 ] ; 2004-07.
Abstract
Severe acute respiratory syndrome (SARS)‐CoV is a newly emerging virus that causes SARS with high mortality rate in infected people. To study the humoral responses against SARS‐CoV, we evaluated nucleocapsid (N) and spike (S) proteins‐specific antibodies in patients' sera by Western blotting and enzyme‐linked immunosorbent assay (ELISA). Recombinant N and S proteins of SARS‐CoV were purified from transformed E. coli. Serum specimens from 40 SARS‐CoV‐infected patients in the convalescent phase were analyzed by Western blotting using the purified antigens. Serial serum specimens from 12 RT‐PCR‐confirmed SARS patients were assayed by ELISA using the recombinant N protein as coated antigen. By Western blotting, 97.5% of the SARS patients were positive for N protein‐specific antibodies whereas only 47.5% of the samples were positive for S protein‐specific antibodies. Using N protein‐based ELISA, 10 out of the 12 patients were positive for N protein‐specific antibodies and 6 of them showed seroconversion at mean of 16 days after onset of fever. Immunoblotting was useful for detecting the humoral immune response after SARS‐CoV infection. Antibodies against SARS‐CoV N protein appear at the early stage of infection, therefore, N protein‐based ELISA could serve as a simple, sensitive, and specific test for diagnosing SARS‐CoV infection. J. Med. Virol. 73:338–346, 2004. © 2004 Wiley‐Liss, Inc.
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DOI: 10.1002/jmv.20096
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Shiou Wei" uniqKey="Yeh S" first="Shiou-Hwei" last="Yeh">Shiou-Hwei Yeh</name><affiliation><mods:affiliation>Division of Molecular and Genomic Medicine, National Health Research Institutes, Taipei, Taiwan</mods:affiliation></affiliation></author><author><name sortKey="Huang, Wen Ung" sort="Huang, Wen Ung" uniqKey="Huang W" first="Wen-Hung" last="Huang">Wen-Hung Huang</name><affiliation><mods:affiliation>Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei, Taiwan</mods:affiliation></affiliation></author><author><name sortKey="Hsueh, Po En" sort="Hsueh, Po En" uniqKey="Hsueh P" first="Po-Ren" last="Hsueh">Po-Ren Hsueh</name><affiliation><mods:affiliation>Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan</mods:affiliation></affiliation></author><author><name sortKey="Yang, Wen Ieh" sort="Yang, Wen Ieh" uniqKey="Yang W" first="Wen-Zieh" last="Yang">Wen-Zieh Yang</name><affiliation><mods:affiliation>Center for Disease Control, Taipei, Taiwan</mods:affiliation></affiliation></author><author><name sortKey="Yang, Jyh Uan" sort="Yang, Jyh Uan" uniqKey="Yang J" first="Jyh-Yuan" last="Yang">Jyh-Yuan Yang</name><affiliation><mods:affiliation>Center for Disease Control, Taipei, Taiwan</mods:affiliation></affiliation></author><author><name sortKey="Su, Ih En" sort="Su, Ih En" uniqKey="Su I" first="Ih-Jen" last="Su">Ih-Jen Su</name><affiliation><mods:affiliation>Center for Disease Control, Taipei, Taiwan</mods:affiliation></affiliation></author><author><name sortKey="Chang, Shan Hwen" sort="Chang, Shan Hwen" uniqKey="Chang S" first="Shan-Chwen" last="Chang">Shan-Chwen Chang</name><affiliation><mods:affiliation>Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan</mods:affiliation></affiliation></author><author><name sortKey="Chen, Pei Er" sort="Chen, Pei Er" uniqKey="Chen P" first="Pei-Jer" last="Chen">Pei-Jer Chen</name><affiliation><mods:affiliation>Graduate Institute 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Immunoblotting was useful for detecting the humoral immune response after SARS‐CoV infection. Antibodies against SARS‐CoV N protein appear at the early stage of infection, therefore, N protein‐based ELISA could serve as a simple, sensitive, and specific test for diagnosing SARS‐CoV infection. J. Med. 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Medicine, National Taiwan University Hospital, Taipei, Taiwan</json:string></affiliations></json:item><json:item><name>Pei‐Jer Chen</name><affiliations><json:string>Graduate Institute of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan</json:string><json:string>Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei, Taiwan</json:string><json:string>Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan</json:string><json:string>E-mail: peijer@ha.mc.ntu.edu.tw</json:string><json:string>Correspondence address: Director, Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, 1 Chang‐Te Street, Taipei 100, Taiwan.===</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>SARS</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>SARS‐CoV</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>ELISA</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>N protein</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>S protein</value></json:item></subject><articleId><json:string>JMV20096</json:string></articleId><arkIstex>ark:/67375/WNG-RLPXX9JD-2</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Severe acute respiratory syndrome (SARS)‐CoV is a newly emerging virus that causes SARS with high mortality rate in infected people. To study the humoral responses against SARS‐CoV, we evaluated nucleocapsid (N) and spike (S) proteins‐specific antibodies in patients' sera by Western blotting and enzyme‐linked immunosorbent assay (ELISA). Recombinant N and S proteins of SARS‐CoV were purified from transformed E. coli. Serum specimens from 40 SARS‐CoV‐infected patients in the convalescent phase were analyzed by Western blotting using the purified antigens. Serial serum specimens from 12 RT‐PCR‐confirmed SARS patients were assayed by ELISA using the recombinant N protein as coated antigen. By Western blotting, 97.5% of the SARS patients were positive for N protein‐specific antibodies whereas only 47.5% of the samples were positive for S protein‐specific antibodies. Using N protein‐based ELISA, 10 out of the 12 patients were positive for N protein‐specific antibodies and 6 of them showed seroconversion at mean of 16 days after onset of fever. Immunoblotting was useful for detecting the humoral immune response after SARS‐CoV infection. Antibodies against SARS‐CoV N protein appear at the early stage of infection, therefore, N protein‐based ELISA could serve as a simple, sensitive, and specific test for diagnosing SARS‐CoV infection. J. Med. Virol. 73:338–346, 2004. © 2004 Wiley‐Liss, Inc.</abstract><qualityIndicators><score>9.472</score><pdfWordCount>5061</pdfWordCount><pdfCharCount>31525</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>9</pdfPageCount><pdfPageSize>594 x 792 pts</pdfPageSize><pdfWordsPerPage>562</pdfWordsPerPage><pdfText>true</pdfText><refBibsNative>true</refBibsNative><abstractWordCount>206</abstractWordCount><abstractCharCount>1405</abstractCharCount><keywordCount>5</keywordCount></qualityIndicators><title>Evaluation of antibody responses against SARS coronaviral nucleocapsid or spike proteins by immunoblotting or ELISA</title><pmid><json:string>15170626</json:string></pmid><genre><json:string>article</json:string></genre><host><title>Journal of Medical Virology</title><language><json:string>unknown</json:string></language><doi><json:string>10.1002/(ISSN)1096-9071</json:string></doi><issn><json:string>0146-6615</json:string></issn><eissn><json:string>1096-9071</json:string></eissn><publisherId><json:string>JMV</json:string></publisherId><volume>73</volume><issue>3</issue><pages><first>338</first><last>346</last><total>9</total></pages><genre><json:string>journal</json:string></genre><subject><json:item><value>Research Article</value></json:item></subject></host><namedEntities><unitex><date><json:string>2002</json:string><json:string>2003</json:string><json:string>2004</json:string></date><geogName></geogName><orgName><json:string>Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan</json:string><json:string>WHO</json:string><json:string>Biotech</json:string><json:string>Division of Molecular</json:string><json:string>PerkinElmer Life Sciences, Inc., Boston</json:string><json:string>Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine</json:string><json:string>Singapore, Vietnam, Canada, America, Taiwan</json:string><json:string>National Health Research Institutes, Taipei, Taiwan</json:string><json:string>Wiley-Liss, Inc</json:string><json:string>PerkinElmer Life Sciences, Inc.</json:string><json:string>LISS, INC.</json:string><json:string>DAKO Corporation</json:string><json:string>General Biologicals Corp.</json:string><json:string>National Taiwan University Hospital</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>ELISA Li-Rung</json:string><json:string>Chi-Ming Chiu</json:string><json:string>Hong Kong</json:string><json:string>HuiLin</json:string><json:string>Chun-Jen Liu</json:string><json:string>Lih-Hwa Hwang</json:string><json:string>J. Med</json:string><json:string>Jen Su</json:string><json:string>Ren Hsueh</json:string><json:string>Pei-Jer Chen</json:string><json:string>Yuan Yang</json:string><json:string>Spike Proteins</json:string><json:string>Viral Antigen</json:string></persName><placeName><json:string>Hong Kong</json:string><json:string>Taiwan</json:string><json:string>Antigenicity</json:string><json:string>Puri</json:string><json:string>York</json:string><json:string>Taipei</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Rota et al., 2003</json:string><json:string>Bergmann et al., 1996</json:string><json:string>Ksiazek et al., 2003</json:string><json:string>Drosten et al., 2003</json:string><json:string>Macnaughton et al., 1981</json:string><json:string>Boots et al., 1992</json:string><json:string>Liu et al., 2001</json:string><json:string>Stohlman et al., 1992</json:string><json:string>Boots et al., 1991</json:string><json:string>Fleming et al., 1983</json:string><json:string>Peiris et al., 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type="first">Li‐Rung</forename><surname>Huang</surname></persName><affiliation><orgName type="institution">Graduate Institute of Microbiology</orgName><orgName type="institution">National Taiwan University College of Medicine</orgName><address><addrLine>Taipei</addrLine><addrLine>Taiwan</addrLine><country key="TW" xml:lang="en">TAIWAN, PROVINCE OF CHINA</country></address></affiliation></author><author xml:id="author-0001"><persName><forename type="first">Chi‐Ming</forename><surname>Chiu</surname></persName><affiliation><orgName type="institution">Graduate Institute of Clinical Medicine</orgName><orgName type="institution">National Taiwan University College of Medicine</orgName><address><addrLine>Taipei</addrLine><addrLine>Taiwan</addrLine><country key="TW" xml:lang="en">TAIWAN, PROVINCE OF CHINA</country></address></affiliation></author><author xml:id="author-0002"><persName><forename type="first">Shiou‐Hwei</forename><surname>Yeh</surname></persName><affiliation><orgName type="division">Division of Molecular and Genomic Medicine</orgName><orgName type="institution">National Health Research Institutes</orgName><address><addrLine>Taipei</addrLine><addrLine>Taiwan</addrLine><country key="TW" xml:lang="en">TAIWAN, PROVINCE OF CHINA</country></address></affiliation></author><author xml:id="author-0003"><persName><forename type="first">Wen‐Hung</forename><surname>Huang</surname></persName><affiliation><orgName type="institution">Graduate Institute of Clinical Medicine</orgName><orgName type="institution">National Taiwan University College of Medicine</orgName><address><addrLine>Taipei</addrLine><addrLine>Taiwan</addrLine><country key="TW" xml:lang="en">TAIWAN, PROVINCE OF CHINA</country></address></affiliation></author><author xml:id="author-0004"><persName><forename type="first">Po‐Ren</forename><surname>Hsueh</surname></persName><affiliation><orgName type="division">Department of Internal Medicine</orgName><orgName type="institution">National Taiwan University Hospital</orgName><address><addrLine>Taipei</addrLine><addrLine>Taiwan</addrLine><country key="TW" xml:lang="en">TAIWAN, PROVINCE OF CHINA</country></address></affiliation></author><author xml:id="author-0005"><persName><forename type="first">Wen‐Zieh</forename><surname>Yang</surname></persName><affiliation><orgName type="institution">Center for Disease Control</orgName><address><addrLine>Taipei</addrLine><addrLine>Taiwan</addrLine><country key="TW" xml:lang="en">TAIWAN, PROVINCE OF CHINA</country></address></affiliation></author><author xml:id="author-0006"><persName><forename type="first">Jyh‐Yuan</forename><surname>Yang</surname></persName><affiliation><orgName type="institution">Center for Disease Control</orgName><address><addrLine>Taipei</addrLine><addrLine>Taiwan</addrLine><country key="TW" xml:lang="en">TAIWAN, PROVINCE OF CHINA</country></address></affiliation></author><author xml:id="author-0007"><persName><forename type="first">Ih‐Jen</forename><surname>Su</surname></persName><affiliation><orgName type="institution">Center for Disease Control</orgName><address><addrLine>Taipei</addrLine><addrLine>Taiwan</addrLine><country key="TW" xml:lang="en">TAIWAN, PROVINCE OF CHINA</country></address></affiliation></author><author xml:id="author-0008"><persName><forename type="first">Shan‐Chwen</forename><surname>Chang</surname></persName><affiliation><orgName type="division">Department of Internal Medicine</orgName><orgName type="institution">National Taiwan University Hospital</orgName><address><addrLine>Taipei</addrLine><addrLine>Taiwan</addrLine><country key="TW" xml:lang="en">TAIWAN, PROVINCE OF CHINA</country></address></affiliation></author><author xml:id="author-0009" role="corresp"><persName><forename type="first">Pei‐Jer</forename><surname>Chen</surname></persName><email>peijer@ha.mc.ntu.edu.tw</email><affiliation><orgName type="institution">Graduate Institute of Microbiology</orgName><orgName 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number="1132"></count></countGroup><titleGroup><title type="main" xml:lang="en">Evaluation of antibody responses against SARS coronaviral nucleocapsid or spike proteins by immunoblotting or ELISA</title><title type="short" xml:lang="en">Antibody Response Against Proteins of SARS‐CoV</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Li‐Rung</givenNames><familyName>Huang</familyName></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Chi‐Ming</givenNames><familyName>Chiu</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#af3"><personName><givenNames>Shiou‐Hwei</givenNames><familyName>Yeh</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Wen‐Hung</givenNames><familyName>Huang</familyName></personName></creator><creator xml:id="au5" 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To study the humoral responses against SARS‐CoV, we evaluated nucleocapsid (N) and spike (S) proteins‐specific antibodies in patients' sera by Western blotting and enzyme‐linked immunosorbent assay (ELISA). Recombinant N and S proteins of SARS‐CoV were purified from transformed <i>E. coli</i>. Serum specimens from 40 SARS‐CoV‐infected patients in the convalescent phase were analyzed by Western blotting using the purified antigens. Serial serum specimens from 12 RT‐PCR‐confirmed SARS patients were assayed by ELISA using the recombinant N protein as coated antigen. By Western blotting, 97.5% of the SARS patients were positive for N protein‐specific antibodies whereas only 47.5% of the samples were positive for S protein‐specific antibodies. Using N protein‐based ELISA, 10 out of the 12 patients were positive for N protein‐specific antibodies and 6 of them showed seroconversion at mean of 16 days after onset of fever. Immunoblotting was useful for detecting the humoral immune response after SARS‐CoV infection. Antibodies against SARS‐CoV N protein appear at the early stage of infection, therefore, N protein‐based ELISA could serve as a simple, sensitive, and specific test for diagnosing SARS‐CoV infection. J. Med. Virol. 73:338–346, 2004. © 2004 Wiley‐Liss, Inc.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Evaluation of antibody responses against SARS coronaviral nucleocapsid or spike proteins by immunoblotting or ELISA</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Antibody Response Against Proteins of SARS‐CoV</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Evaluation of antibody responses against SARS coronaviral nucleocapsid or spike proteins by immunoblotting or ELISA</title></titleInfo><name type="personal"><namePart type="given">Li‐Rung</namePart><namePart type="family">Huang</namePart><affiliation>Graduate Institute of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Chi‐Ming</namePart><namePart type="family">Chiu</namePart><affiliation>Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei, Taiwan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Shiou‐Hwei</namePart><namePart type="family">Yeh</namePart><affiliation>Division of Molecular and Genomic Medicine, National Health Research Institutes, Taipei, Taiwan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Wen‐Hung</namePart><namePart type="family">Huang</namePart><affiliation>Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei, Taiwan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Po‐Ren</namePart><namePart type="family">Hsueh</namePart><affiliation>Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Wen‐Zieh</namePart><namePart type="family">Yang</namePart><affiliation>Center for Disease Control, Taipei, Taiwan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jyh‐Yuan</namePart><namePart type="family">Yang</namePart><affiliation>Center for Disease Control, Taipei, Taiwan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ih‐Jen</namePart><namePart type="family">Su</namePart><affiliation>Center for Disease Control, Taipei, Taiwan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Shan‐Chwen</namePart><namePart type="family">Chang</namePart><affiliation>Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Pei‐Jer</namePart><namePart type="family">Chen</namePart><affiliation>Graduate Institute of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan</affiliation><affiliation>Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei, Taiwan</affiliation><affiliation>Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan</affiliation><affiliation>E-mail: peijer@ha.mc.ntu.edu.tw</affiliation><affiliation>Correspondence address: Director, Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, 1 Chang‐Te Street, Taipei 100, Taiwan.===</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">2004-07</dateIssued><dateValid encoding="w3cdtf">2004-03-15</dateValid><copyrightDate encoding="w3cdtf">2004</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="figures">4</extent><extent unit="tables">0</extent><extent unit="references">27</extent><extent unit="words">1132</extent></physicalDescription><abstract lang="en">Severe acute respiratory syndrome (SARS)‐CoV is a newly emerging virus that causes SARS with high mortality rate in infected people. To study the humoral responses against SARS‐CoV, we evaluated nucleocapsid (N) and spike (S) proteins‐specific antibodies in patients' sera by Western blotting and enzyme‐linked immunosorbent assay (ELISA). Recombinant N and S proteins of SARS‐CoV were purified from transformed E. coli. Serum specimens from 40 SARS‐CoV‐infected patients in the convalescent phase were analyzed by Western blotting using the purified antigens. Serial serum specimens from 12 RT‐PCR‐confirmed SARS patients were assayed by ELISA using the recombinant N protein as coated antigen. By Western blotting, 97.5% of the SARS patients were positive for N protein‐specific antibodies whereas only 47.5% of the samples were positive for S protein‐specific antibodies. Using N protein‐based ELISA, 10 out of the 12 patients were positive for N protein‐specific antibodies and 6 of them showed seroconversion at mean of 16 days after onset of fever. Immunoblotting was useful for detecting the humoral immune response after SARS‐CoV infection. Antibodies against SARS‐CoV N protein appear at the early stage of infection, therefore, N protein‐based ELISA could serve as a simple, sensitive, and specific test for diagnosing SARS‐CoV infection. J. Med. Virol. 73:338–346, 2004. © 2004 Wiley‐Liss, Inc.</abstract><note type="funding">National Science Council, Taiwan - No. NSC92‐2751‐B‐400‐002‐Y; </note><subject lang="en"><genre>keywords</genre><topic>SARS</topic><topic>SARS‐CoV</topic><topic>ELISA</topic><topic>N protein</topic><topic>S protein</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Medical Virology</title></titleInfo><titleInfo type="abbreviated"><title>J. Med. Virol.</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><subject><genre>article-category</genre><topic>Research Article</topic></subject><identifier type="ISSN">0146-6615</identifier><identifier type="eISSN">1096-9071</identifier><identifier type="DOI">10.1002/(ISSN)1096-9071</identifier><identifier type="PublisherID">JMV</identifier><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>73</number></detail><detail type="issue"><caption>no.</caption><number>3</number></detail><extent unit="pages"><start>338</start><end>346</end><total>9</total></extent></part></relatedItem><relatedItem type="references" displayLabel="cit1"><titleInfo><title>Interactions between coronavirus nucleocapsid protein and viral RNAs: Implications for viral transcription</title></titleInfo><name type="personal"><namePart type="given">RS</namePart><namePart type="family">Baric</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">GW</namePart><namePart type="family">Nelson</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JO</namePart><namePart type="family">Fleming</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">RJ</namePart><namePart type="family">Deans</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JG</namePart><namePart type="family">Keck</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">N</namePart><namePart type="family">Casteel</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">SA</namePart><namePart type="family">Stohlman</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Baric RS, Nelson GW, Fleming JO, Deans RJ, Keck JG, Casteel N, Stohlman SA. 1988. 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