Evaluation of a peptide‐based enzyme immunoassay for anti‐SARS coronavirus IgG antibody
Identifieur interne : 001F67 ( Istex/Checkpoint ); précédent : 001F66; suivant : 001F68Evaluation of a peptide‐based enzyme immunoassay for anti‐SARS coronavirus IgG antibody
Auteurs : Paul K. S. Chan [République populaire de Chine, Hong Kong] ; W. K. To [République populaire de Chine] ; Esther Y. M. Liu [République populaire de Chine] ; T. K. Ng [République populaire de Chine] ; John S. Tam [République populaire de Chine] ; Joseph J. Y. Sung [République populaire de Chine] ; Jean-Michel Lacroix [Canada] ; Michel Houde [Canada]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 2004-12.
English descriptors
- Teeft :
- Absorbance indices, Amino acids, Assay, Chan, Chinese university, Coronavirus, Disease control, Enzyme immunoassay, Healthy controls, High degree, Hong kong, Index values, Indirect assay, Infectious diseases, Large number, Microplate enzyme immunoassay, Novel coronavirus, Peptide, Respiratory syndrome, Sars, Sars group, Sars patients, Sars study group, Serum specimens, Spike protein, Spike proteins, Syndrome, Synthetic peptides, Various control groups, Wales hospital, World health organization.
Abstract
High throughput assays for anti‐SARS‐CoV IgG antibody detection are need for large‐scale epidemiologic studies. The performance of a microplate enzyme immunoassay, DETECT‐SARS™, was evaluated for the detection of anti‐SARS‐CoV IgG antibody. This assay is based on synthetic peptides derived from the nucleocapsid and spike proteins. The results showed that the assay provided a high degree of sensitivity (95.9%) for convalescent serum samples. The level of specificity was close to 90%, and did not show significant variation among different control groups. The high degree of sensitivity together with the high‐throughput nature makes it advantageous as a screening assay for studies where handling of a large number of specimens is required. J. Med. Virol. 74:517–520, 2004. © 2004 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jmv.20207
Affiliations:
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<front><div type="abstract" xml:lang="en">High throughput assays for anti‐SARS‐CoV IgG antibody detection are need for large‐scale epidemiologic studies. The performance of a microplate enzyme immunoassay, DETECT‐SARS™, was evaluated for the detection of anti‐SARS‐CoV IgG antibody. This assay is based on synthetic peptides derived from the nucleocapsid and spike proteins. The results showed that the assay provided a high degree of sensitivity (95.9%) for convalescent serum samples. The level of specificity was close to 90%, and did not show significant variation among different control groups. The high degree of sensitivity together with the high‐throughput nature makes it advantageous as a screening assay for studies where handling of a large number of specimens is required. J. Med. Virol. 74:517–520, 2004. © 2004 Wiley‐Liss, Inc.</div>
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