Evaluation of a peptide‐based enzyme immunoassay for anti‐SARS coronavirus IgG antibody
Identifieur interne : 000990 ( Istex/Curation ); précédent : 000989; suivant : 000991Evaluation of a peptide‐based enzyme immunoassay for anti‐SARS coronavirus IgG antibody
Auteurs : Paul K. S. Chan [République populaire de Chine, Hong Kong] ; W. K. To [République populaire de Chine] ; Esther Y. M. Liu [République populaire de Chine] ; T. K. Ng [République populaire de Chine] ; John S. Tam [République populaire de Chine] ; Joseph J. Y. Sung [République populaire de Chine] ; Jean-Michel Lacroix [Canada] ; Michel Houde [Canada]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 2004-12.
English descriptors
- Teeft :
- Absorbance indices, Amino acids, Assay, Chan, Chinese university, Coronavirus, Disease control, Enzyme immunoassay, Healthy controls, High degree, Hong kong, Index values, Indirect assay, Infectious diseases, Large number, Microplate enzyme immunoassay, Novel coronavirus, Peptide, Respiratory syndrome, Sars, Sars group, Sars patients, Sars study group, Serum specimens, Spike protein, Spike proteins, Syndrome, Synthetic peptides, Various control groups, Wales hospital, World health organization.
Abstract
High throughput assays for anti‐SARS‐CoV IgG antibody detection are need for large‐scale epidemiologic studies. The performance of a microplate enzyme immunoassay, DETECT‐SARS™, was evaluated for the detection of anti‐SARS‐CoV IgG antibody. This assay is based on synthetic peptides derived from the nucleocapsid and spike proteins. The results showed that the assay provided a high degree of sensitivity (95.9%) for convalescent serum samples. The level of specificity was close to 90%, and did not show significant variation among different control groups. The high degree of sensitivity together with the high‐throughput nature makes it advantageous as a screening assay for studies where handling of a large number of specimens is required. J. Med. Virol. 74:517–520, 2004. © 2004 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jmv.20207
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Sung</name><affiliation wicri:level="1"><mods:affiliation>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea></affiliation></author><author><name sortKey="Lacroix, Jean Ichel" sort="Lacroix, Jean Ichel" uniqKey="Lacroix J" first="Jean-Michel" last="Lacroix">Jean-Michel Lacroix</name><affiliation wicri:level="1"><mods:affiliation>Adaltis Development, Inc., Montreal, Quebec, Canada</mods:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Adaltis Development, Inc., Montreal, Quebec</wicri:regionArea></affiliation></author><author><name sortKey="Houde, Michel" sort="Houde, Michel" uniqKey="Houde M" first="Michel" last="Houde">Michel Houde</name><affiliation wicri:level="1"><mods:affiliation>Adaltis Development, Inc., Montreal, Quebec, Canada</mods:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Adaltis Development, Inc., Montreal, Quebec</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:0739D6E4A307DF53586757BAC1A3D2894AF8E248</idno><date when="2004" year="2004">2004</date><idno type="doi">10.1002/jmv.20207</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-2B8N0VCT-C/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000990</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000990</idno><idno type="wicri:Area/Istex/Curation">000990</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Evaluation of a peptide‐based enzyme immunoassay for anti‐SARS coronavirus IgG antibody</title><author><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K. S." last="Chan">Paul K. S. Chan</name><affiliation wicri:level="1"><mods:affiliation>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>E-mail: paulkschan@cuhk.edu.hk</mods:affiliation><country wicri:rule="url">Hong Kong</country></affiliation><affiliation wicri:level="1"><mods:affiliation>Correspondence address: Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China.===</mods:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Correspondence address: Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea></affiliation></author><author><name sortKey="To, W K" sort="To, W K" uniqKey="To W" first="W. K." last="To">W. K. To</name><affiliation wicri:level="1"><mods:affiliation>Department of Pathology, Princess Margaret Hospital, Kowloon, Shatin, New Territories, Hong Kong SAR, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathology, Princess Margaret Hospital, Kowloon, Shatin, New Territories, Hong Kong SAR</wicri:regionArea></affiliation></author><author><name sortKey="Liu, Esther Y M" sort="Liu, Esther Y M" uniqKey="Liu E" first="Esther Y. M." last="Liu">Esther Y. M. Liu</name><affiliation wicri:level="1"><mods:affiliation>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea></affiliation></author><author><name sortKey="Ng, T K" sort="Ng, T K" uniqKey="Ng T" first="T. K." last="Ng">T. K. Ng</name><affiliation wicri:level="1"><mods:affiliation>Department of Pathology, Princess Margaret Hospital, Kowloon, Shatin, New Territories, Hong Kong SAR, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathology, Princess Margaret Hospital, Kowloon, Shatin, New Territories, Hong Kong SAR</wicri:regionArea></affiliation></author><author><name sortKey="Tam, John S" sort="Tam, John S" uniqKey="Tam J" first="John S." last="Tam">John S. Tam</name><affiliation wicri:level="1"><mods:affiliation>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea></affiliation></author><author><name sortKey="Sung, Joseph J Y" sort="Sung, Joseph J Y" uniqKey="Sung J" first="Joseph J. Y." last="Sung">Joseph J. Y. Sung</name><affiliation wicri:level="1"><mods:affiliation>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea></affiliation></author><author><name sortKey="Lacroix, Jean Ichel" sort="Lacroix, Jean Ichel" uniqKey="Lacroix J" first="Jean-Michel" last="Lacroix">Jean-Michel Lacroix</name><affiliation wicri:level="1"><mods:affiliation>Adaltis Development, Inc., Montreal, Quebec, Canada</mods:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Adaltis Development, Inc., Montreal, Quebec</wicri:regionArea></affiliation></author><author><name sortKey="Houde, Michel" sort="Houde, Michel" uniqKey="Houde M" first="Michel" last="Houde">Michel Houde</name><affiliation wicri:level="1"><mods:affiliation>Adaltis Development, Inc., Montreal, Quebec, Canada</mods:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Adaltis Development, Inc., Montreal, Quebec</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Medical Virology</title><title level="j" type="alt">JOURNAL OF MEDICAL VIROLOGY</title><idno type="ISSN">0146-6615</idno><idno type="eISSN">1096-9071</idno><imprint><biblScope unit="vol">74</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="517">517</biblScope><biblScope unit="page" to="520">520</biblScope><biblScope unit="page-count">4</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2004-12">2004-12</date></imprint><idno type="ISSN">0146-6615</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0146-6615</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Absorbance indices</term><term>Amino acids</term><term>Assay</term><term>Chan</term><term>Chinese university</term><term>Coronavirus</term><term>Disease control</term><term>Enzyme immunoassay</term><term>Healthy controls</term><term>High degree</term><term>Hong kong</term><term>Index values</term><term>Indirect assay</term><term>Infectious diseases</term><term>Large number</term><term>Microplate enzyme immunoassay</term><term>Novel coronavirus</term><term>Peptide</term><term>Respiratory syndrome</term><term>Sars</term><term>Sars group</term><term>Sars patients</term><term>Sars study group</term><term>Serum specimens</term><term>Spike protein</term><term>Spike proteins</term><term>Syndrome</term><term>Synthetic peptides</term><term>Various control groups</term><term>Wales hospital</term><term>World health organization</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">High throughput assays for anti‐SARS‐CoV IgG antibody detection are need for large‐scale epidemiologic studies. The performance of a microplate enzyme immunoassay, DETECT‐SARS™, was evaluated for the detection of anti‐SARS‐CoV IgG antibody. This assay is based on synthetic peptides derived from the nucleocapsid and spike proteins. The results showed that the assay provided a high degree of sensitivity (95.9%) for convalescent serum samples. The level of specificity was close to 90%, and did not show significant variation among different control groups. The high degree of sensitivity together with the high‐throughput nature makes it advantageous as a screening assay for studies where handling of a large number of specimens is required. J. Med. Virol. 74:517–520, 2004. © 2004 Wiley‐Liss, Inc.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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