Fgf signaling in the zebrafish adult brain: association of Fgf activity with ventricular zones but not cell proliferation.
Identifieur interne : 000152 ( Hal/Checkpoint ); précédent : 000151; suivant : 000153Fgf signaling in the zebrafish adult brain: association of Fgf activity with ventricular zones but not cell proliferation.
Auteurs : Stefanie Topp [Allemagne] ; Christian Stigloher [Allemagne] ; Anna Z. Komisarczuk [Norvège] ; Birgit Adolf [Allemagne] ; Thomas S. Becker [Norvège] ; Laure Bally-Cuif [Allemagne]Source :
- Journal of Comparative Neurology [ 0092-7317 ] ; 2008-10-01.
Abstract
The zebrafish adult brain contains numerous neural progenitors and is a good model to approach the general mechanisms of adult neural stem cell maintenance and neurogenesis. Here we use this model to test for a correlation between Fgf signaling and cell proliferation in adult progenitor zones. We report expression of Fgf signals (fgf3,4,8a,8b,17b), receptors (fgfr1-4), and targets (erm, pea3, dusp6, spry1,2,4, and P-ERK) and document that genes of the embryonic fgf8 synexpression group acquire strikingly divergent patterns in the adult brain. We further document the specific expression of fgf3, fgfr1-3, dusp6, and P-ERK in ventricular zones, which contain neural progenitors. In these locations, however, a comparison at the single-cell level of fgfr/P-ERK expression with bromo-deoxy-uridine (BrdU) incorporation and the proliferation marker MCM5 indicates that Fgf signaling is not specifically associated with proliferating progenitors. Rather, it correlates with the ventricular radial glia state, some of which only are progenitors. Together these results stress the importance of Fgf signaling in the adult brain and establish the basis to study its function in zebrafish, in particular in relation to adult neurogenesis.
Url:
DOI: 10.1002/cne.21802
Links toward previous steps (curation, corpus...)
Links to Exploration step
Hal:hal-01055260Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Fgf signaling in the zebrafish adult brain: association of Fgf activity with ventricular zones but not cell proliferation.</title><author><name sortKey="Topp, Stefanie" sort="Topp, Stefanie" uniqKey="Topp S" first="Stefanie" last="Topp">Stefanie Topp</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-90936" status="INCOMING"> <orgName>Department of Zebrafish Neurogenetics, Institute of Developmental Genetics</orgName> <desc> <address> <addrLine>Ingolstaedter Landstrasse 1, D85764, Neuherberg</addrLine> <country key="DE"></country> </address> </desc> <listRelation> <relation active="#struct-317924" type="direct"></relation> <relation active="#struct-350940" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-317924" type="direct"><org type="institution" xml:id="struct-317924" status="INCOMING"> <orgName>German Research Center for Environmental Health</orgName> <desc> <address> <country key="FR"></country> </address> </desc> </org></tutelle><tutelle active="#struct-350940" type="direct"><org type="institution" xml:id="struct-350940" status="VALID"> <orgName>Helmholtz-Zentrum München </orgName> <orgName type="acronym">HZM</orgName> <desc> <address> <addrLine>Ingolstaedter Landstraße 1, D-85764 Neuherberg</addrLine> <country key="DE"></country> </address> <ref type="url">https://www.helmholtz-muenchen.de/en/index.html</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Allemagne</country></affiliation></author><author><name sortKey="Stigloher, Christian" sort="Stigloher, Christian" uniqKey="Stigloher C" first="Christian" last="Stigloher">Christian Stigloher</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-90936" status="INCOMING"> <orgName>Department of Zebrafish Neurogenetics, Institute of Developmental Genetics</orgName> <desc> <address> <addrLine>Ingolstaedter Landstrasse 1, D85764, Neuherberg</addrLine> <country key="DE"></country> </address> </desc> <listRelation> <relation active="#struct-317924" type="direct"></relation> <relation active="#struct-350940" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-317924" type="direct"><org type="institution" xml:id="struct-317924" status="INCOMING"> <orgName>German Research Center for Environmental Health</orgName> <desc> <address> <country key="FR"></country> </address> </desc> </org></tutelle><tutelle active="#struct-350940" type="direct"><org type="institution" xml:id="struct-350940" status="VALID"> <orgName>Helmholtz-Zentrum München </orgName> <orgName type="acronym">HZM</orgName> <desc> <address> <addrLine>Ingolstaedter Landstraße 1, D-85764 Neuherberg</addrLine> <country key="DE"></country> </address> <ref type="url">https://www.helmholtz-muenchen.de/en/index.html</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Allemagne</country></affiliation></author><author><name sortKey="Komisarczuk, Anna Z" sort="Komisarczuk, Anna Z" uniqKey="Komisarczuk A" first="Anna Z" last="Komisarczuk">Anna Z. Komisarczuk</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-51800" status="INCOMING"> <orgName>Sars International Centre for Marine Molecular Biology</orgName> <desc> <address> <addrLine>Bergen High Technology Centre, Bergen</addrLine> <country key="NO"></country> </address> </desc> <listRelation> <relation active="#struct-300728" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-300728" type="direct"><org type="institution" xml:id="struct-300728" status="VALID"> <orgName>University of Bergen</orgName> <orgName type="acronym">UIB</orgName> <desc> <address> <addrLine>Postboks 7800, NO-5020 Bergen</addrLine> <country key="NO"></country> </address> <ref type="url">http://www.uib.no/en</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Norvège</country></affiliation></author><author><name sortKey="Adolf, Birgit" sort="Adolf, Birgit" uniqKey="Adolf B" first="Birgit" last="Adolf">Birgit Adolf</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-90936" status="INCOMING"> <orgName>Department of Zebrafish Neurogenetics, Institute of Developmental Genetics</orgName> <desc> <address> <addrLine>Ingolstaedter Landstrasse 1, D85764, Neuherberg</addrLine> <country key="DE"></country> </address> </desc> <listRelation> <relation active="#struct-317924" type="direct"></relation> <relation active="#struct-350940" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-317924" type="direct"><org type="institution" xml:id="struct-317924" status="INCOMING"> <orgName>German Research Center for Environmental Health</orgName> <desc> <address> <country key="FR"></country> </address> </desc> </org></tutelle><tutelle active="#struct-350940" type="direct"><org type="institution" xml:id="struct-350940" status="VALID"> <orgName>Helmholtz-Zentrum München </orgName> <orgName type="acronym">HZM</orgName> <desc> <address> <addrLine>Ingolstaedter Landstraße 1, D-85764 Neuherberg</addrLine> <country key="DE"></country> </address> <ref type="url">https://www.helmholtz-muenchen.de/en/index.html</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Allemagne</country></affiliation></author><author><name sortKey="Becker, Thomas S" sort="Becker, Thomas S" uniqKey="Becker T" first="Thomas S" last="Becker">Thomas S. Becker</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-51800" status="INCOMING"> <orgName>Sars International Centre for Marine Molecular Biology</orgName> <desc> <address> <addrLine>Bergen High Technology Centre, Bergen</addrLine> <country key="NO"></country> </address> </desc> <listRelation> <relation active="#struct-300728" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-300728" type="direct"><org type="institution" xml:id="struct-300728" status="VALID"> <orgName>University of Bergen</orgName> <orgName type="acronym">UIB</orgName> <desc> <address> <addrLine>Postboks 7800, NO-5020 Bergen</addrLine> <country key="NO"></country> </address> <ref type="url">http://www.uib.no/en</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Norvège</country></affiliation></author><author><name sortKey="Bally Cuif, Laure" sort="Bally Cuif, Laure" uniqKey="Bally Cuif L" first="Laure" last="Bally-Cuif">Laure Bally-Cuif</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-90936" status="INCOMING"> <orgName>Department of Zebrafish Neurogenetics, Institute of Developmental Genetics</orgName> <desc> <address> <addrLine>Ingolstaedter Landstrasse 1, D85764, Neuherberg</addrLine> <country key="DE"></country> </address> </desc> <listRelation> <relation active="#struct-317924" type="direct"></relation> <relation active="#struct-350940" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-317924" type="direct"><org type="institution" xml:id="struct-317924" status="INCOMING"> <orgName>German Research Center for Environmental Health</orgName> <desc> <address> <country key="FR"></country> </address> </desc> </org></tutelle><tutelle active="#struct-350940" type="direct"><org type="institution" xml:id="struct-350940" status="VALID"> <orgName>Helmholtz-Zentrum München </orgName> <orgName type="acronym">HZM</orgName> <desc> <address> <addrLine>Ingolstaedter Landstraße 1, D-85764 Neuherberg</addrLine> <country key="DE"></country> </address> <ref type="url">https://www.helmholtz-muenchen.de/en/index.html</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Allemagne</country></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">HAL</idno><idno type="RBID">Hal:hal-01055260</idno><idno type="halId">hal-01055260</idno><idno type="halUri">https://hal.archives-ouvertes.fr/hal-01055260</idno><idno type="url">https://hal.archives-ouvertes.fr/hal-01055260</idno><idno type="doi">10.1002/cne.21802</idno><date when="2008-10-01">2008-10-01</date><idno type="wicri:Area/Hal/Corpus">000067</idno><idno type="wicri:Area/Hal/Curation">000067</idno><idno type="wicri:Area/Hal/Checkpoint">000152</idno><idno type="wicri:explorRef" wicri:stream="Hal" wicri:step="Checkpoint">000152</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Fgf signaling in the zebrafish adult brain: association of Fgf activity with ventricular zones but not cell proliferation.</title><author><name sortKey="Topp, Stefanie" sort="Topp, Stefanie" uniqKey="Topp S" first="Stefanie" last="Topp">Stefanie Topp</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-90936" status="INCOMING"> <orgName>Department of Zebrafish Neurogenetics, Institute of Developmental Genetics</orgName> <desc> <address> <addrLine>Ingolstaedter Landstrasse 1, D85764, Neuherberg</addrLine> <country key="DE"></country> </address> </desc> <listRelation> <relation active="#struct-317924" type="direct"></relation> <relation active="#struct-350940" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-317924" type="direct"><org type="institution" xml:id="struct-317924" status="INCOMING"> <orgName>German Research Center for Environmental Health</orgName> <desc> <address> <country key="FR"></country> </address> </desc> </org></tutelle><tutelle active="#struct-350940" type="direct"><org type="institution" xml:id="struct-350940" status="VALID"> <orgName>Helmholtz-Zentrum München </orgName> <orgName type="acronym">HZM</orgName> <desc> <address> <addrLine>Ingolstaedter Landstraße 1, D-85764 Neuherberg</addrLine> <country key="DE"></country> </address> <ref type="url">https://www.helmholtz-muenchen.de/en/index.html</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Allemagne</country></affiliation></author><author><name sortKey="Stigloher, Christian" sort="Stigloher, Christian" uniqKey="Stigloher C" first="Christian" last="Stigloher">Christian Stigloher</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-90936" status="INCOMING"> <orgName>Department of Zebrafish Neurogenetics, Institute of Developmental Genetics</orgName> <desc> <address> <addrLine>Ingolstaedter Landstrasse 1, D85764, Neuherberg</addrLine> <country key="DE"></country> </address> </desc> <listRelation> <relation active="#struct-317924" type="direct"></relation> <relation active="#struct-350940" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-317924" type="direct"><org type="institution" xml:id="struct-317924" status="INCOMING"> <orgName>German Research Center for Environmental Health</orgName> <desc> <address> <country key="FR"></country> </address> </desc> </org></tutelle><tutelle active="#struct-350940" type="direct"><org type="institution" xml:id="struct-350940" status="VALID"> <orgName>Helmholtz-Zentrum München </orgName> <orgName type="acronym">HZM</orgName> <desc> <address> <addrLine>Ingolstaedter Landstraße 1, D-85764 Neuherberg</addrLine> <country key="DE"></country> </address> <ref type="url">https://www.helmholtz-muenchen.de/en/index.html</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Allemagne</country></affiliation></author><author><name sortKey="Komisarczuk, Anna Z" sort="Komisarczuk, Anna Z" uniqKey="Komisarczuk A" first="Anna Z" last="Komisarczuk">Anna Z. Komisarczuk</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-51800" status="INCOMING"> <orgName>Sars International Centre for Marine Molecular Biology</orgName> <desc> <address> <addrLine>Bergen High Technology Centre, Bergen</addrLine> <country key="NO"></country> </address> </desc> <listRelation> <relation active="#struct-300728" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-300728" type="direct"><org type="institution" xml:id="struct-300728" status="VALID"> <orgName>University of Bergen</orgName> <orgName type="acronym">UIB</orgName> <desc> <address> <addrLine>Postboks 7800, NO-5020 Bergen</addrLine> <country key="NO"></country> </address> <ref type="url">http://www.uib.no/en</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Norvège</country></affiliation></author><author><name sortKey="Adolf, Birgit" sort="Adolf, Birgit" uniqKey="Adolf B" first="Birgit" last="Adolf">Birgit Adolf</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-90936" status="INCOMING"> <orgName>Department of Zebrafish Neurogenetics, Institute of Developmental Genetics</orgName> <desc> <address> <addrLine>Ingolstaedter Landstrasse 1, D85764, Neuherberg</addrLine> <country key="DE"></country> </address> </desc> <listRelation> <relation active="#struct-317924" type="direct"></relation> <relation active="#struct-350940" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-317924" type="direct"><org type="institution" xml:id="struct-317924" status="INCOMING"> <orgName>German Research Center for Environmental Health</orgName> <desc> <address> <country key="FR"></country> </address> </desc> </org></tutelle><tutelle active="#struct-350940" type="direct"><org type="institution" xml:id="struct-350940" status="VALID"> <orgName>Helmholtz-Zentrum München </orgName> <orgName type="acronym">HZM</orgName> <desc> <address> <addrLine>Ingolstaedter Landstraße 1, D-85764 Neuherberg</addrLine> <country key="DE"></country> </address> <ref type="url">https://www.helmholtz-muenchen.de/en/index.html</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Allemagne</country></affiliation></author><author><name sortKey="Becker, Thomas S" sort="Becker, Thomas S" uniqKey="Becker T" first="Thomas S" last="Becker">Thomas S. Becker</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-51800" status="INCOMING"> <orgName>Sars International Centre for Marine Molecular Biology</orgName> <desc> <address> <addrLine>Bergen High Technology Centre, Bergen</addrLine> <country key="NO"></country> </address> </desc> <listRelation> <relation active="#struct-300728" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-300728" type="direct"><org type="institution" xml:id="struct-300728" status="VALID"> <orgName>University of Bergen</orgName> <orgName type="acronym">UIB</orgName> <desc> <address> <addrLine>Postboks 7800, NO-5020 Bergen</addrLine> <country key="NO"></country> </address> <ref type="url">http://www.uib.no/en</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Norvège</country></affiliation></author><author><name sortKey="Bally Cuif, Laure" sort="Bally Cuif, Laure" uniqKey="Bally Cuif L" first="Laure" last="Bally-Cuif">Laure Bally-Cuif</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-90936" status="INCOMING"> <orgName>Department of Zebrafish Neurogenetics, Institute of Developmental Genetics</orgName> <desc> <address> <addrLine>Ingolstaedter Landstrasse 1, D85764, Neuherberg</addrLine> <country key="DE"></country> </address> </desc> <listRelation> <relation active="#struct-317924" type="direct"></relation> <relation active="#struct-350940" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-317924" type="direct"><org type="institution" xml:id="struct-317924" status="INCOMING"> <orgName>German Research Center for Environmental Health</orgName> <desc> <address> <country key="FR"></country> </address> </desc> </org></tutelle><tutelle active="#struct-350940" type="direct"><org type="institution" xml:id="struct-350940" status="VALID"> <orgName>Helmholtz-Zentrum München </orgName> <orgName type="acronym">HZM</orgName> <desc> <address> <addrLine>Ingolstaedter Landstraße 1, D-85764 Neuherberg</addrLine> <country key="DE"></country> </address> <ref type="url">https://www.helmholtz-muenchen.de/en/index.html</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Allemagne</country></affiliation></author></analytic><idno type="DOI">10.1002/cne.21802</idno><series><title level="j">Journal of Comparative Neurology</title><idno type="ISSN">0092-7317</idno><imprint><date type="datePub">2008-10-01</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"> <p>The zebrafish adult brain contains numerous neural progenitors and is a good model to approach the general mechanisms of adult neural stem cell maintenance and neurogenesis. Here we use this model to test for a correlation between Fgf signaling and cell proliferation in adult progenitor zones. We report expression of Fgf signals (fgf3,4,8a,8b,17b), receptors (fgfr1-4), and targets (erm, pea3, dusp6, spry1,2,4, and P-ERK) and document that genes of the embryonic fgf8 synexpression group acquire strikingly divergent patterns in the adult brain. We further document the specific expression of fgf3, fgfr1-3, dusp6, and P-ERK in ventricular zones, which contain neural progenitors. In these locations, however, a comparison at the single-cell level of fgfr/P-ERK expression with bromo-deoxy-uridine (BrdU) incorporation and the proliferation marker MCM5 indicates that Fgf signaling is not specifically associated with proliferating progenitors. Rather, it correlates with the ventricular radial glia state, some of which only are progenitors. Together these results stress the importance of Fgf signaling in the adult brain and establish the basis to study its function in zebrafish, in particular in relation to adult neurogenesis.</p> </div></front></TEI><hal api="V3"> <titleStmt> <title xml:lang="en">Fgf signaling in the zebrafish adult brain: association of Fgf activity with ventricular zones but not cell proliferation.</title> <author role="aut"> <persName> <forename type="first">Stefanie</forename> <surname>Topp</surname> </persName> <idno type="halauthorid">514202</idno> <affiliation ref="#struct-90936"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Christian</forename> <surname>Stigloher</surname> </persName> <idno type="halauthorid">514186</idno> <affiliation ref="#struct-90936"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Anna Z</forename> <surname>Komisarczuk</surname> </persName> <idno type="halauthorid">228914</idno> <affiliation ref="#struct-51800"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Birgit</forename> <surname>Adolf</surname> </persName> <idno type="halauthorid">228927</idno> <affiliation ref="#struct-90936"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Thomas S</forename> <surname>Becker</surname> </persName> <idno type="halauthorid">228930</idno> <affiliation ref="#struct-51800"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Laure</forename> <surname>Bally-Cuif</surname> </persName> <idno type="halauthorid">395856</idno> <affiliation ref="#struct-90936"></affiliation> </author> <editor role="depositor"> <persName> <forename>Alain</forename> <surname>PERIGNON</surname> </persName> <email type="md5">0915a8bc0b5a494fc86eb9724296445c</email> <email type="domain">cnrs.fr</email> </editor> </titleStmt> <editionStmt> <edition n="v1" type="current"> <date type="whenSubmitted">2014-08-12 10:16:13</date> <date type="whenModified">2018-04-15 20:40:17</date> <date type="whenReleased">2014-08-12 10:16:13</date> <date type="whenProduced">2008-10-01</date> <ref type="externalLink" target="https://api.istex.fr/ark:/67375/WNG-LRHKWRLG-S/fulltext.pdf?sid=hal"></ref> </edition> <respStmt> <resp>contributor</resp> <name key="108746"> <persName> <forename>Alain</forename> <surname>PERIGNON</surname> </persName> <email type="md5">0915a8bc0b5a494fc86eb9724296445c</email> <email type="domain">cnrs.fr</email> </name> </respStmt> </editionStmt> <publicationStmt> <distributor>CCSD</distributor> <idno type="halId">hal-01055260</idno> <idno type="halUri">https://hal.archives-ouvertes.fr/hal-01055260</idno> <idno type="halBibtex">topp:hal-01055260</idno> <idno type="halRefHtml">Journal of Comparative Neurology, John Wiley & Sons, 2008, 510 (4), pp.422-39. ⟨10.1002/cne.21802⟩</idno> <idno type="halRef">Journal of Comparative Neurology, John Wiley & Sons, 2008, 510 (4), pp.422-39. ⟨10.1002/cne.21802⟩</idno> </publicationStmt> <seriesStmt></seriesStmt> <notesStmt> <note type="audience" n="2">International</note> <note type="popular" n="0">No</note> <note type="peer" n="1">Yes</note> </notesStmt> <sourceDesc> <biblStruct> <analytic> <title xml:lang="en">Fgf signaling in the zebrafish adult brain: association of Fgf activity with ventricular zones but not cell proliferation.</title> <author role="aut"> <persName> <forename type="first">Stefanie</forename> <surname>Topp</surname> </persName> <idno type="halauthorid">514202</idno> <affiliation ref="#struct-90936"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Christian</forename> <surname>Stigloher</surname> </persName> <idno type="halauthorid">514186</idno> <affiliation ref="#struct-90936"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Anna Z</forename> <surname>Komisarczuk</surname> </persName> <idno type="halauthorid">228914</idno> <affiliation ref="#struct-51800"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Birgit</forename> <surname>Adolf</surname> </persName> <idno type="halauthorid">228927</idno> <affiliation ref="#struct-90936"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Thomas S</forename> <surname>Becker</surname> </persName> <idno type="halauthorid">228930</idno> <affiliation ref="#struct-51800"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Laure</forename> <surname>Bally-Cuif</surname> </persName> <idno type="halauthorid">395856</idno> <affiliation ref="#struct-90936"></affiliation> </author> </analytic> <monogr> <idno type="halJournalId" status="VALID">15260</idno> <idno type="issn">0092-7317</idno> <idno type="eissn">1550-7130</idno> <title level="j">Journal of Comparative Neurology</title> <imprint> <publisher>John Wiley & Sons</publisher> <biblScope unit="volume">510</biblScope> <biblScope unit="issue">4</biblScope> <biblScope unit="pp">422-39</biblScope> <date type="datePub">2008-10-01</date> </imprint> </monogr> <idno type="doi">10.1002/cne.21802</idno> <idno type="pubmed">18666124</idno> </biblStruct> </sourceDesc> <profileDesc> <langUsage> <language ident="en">English</language> </langUsage> <textClass> <classCode scheme="mesh">Animals</classCode> <classCode scheme="mesh">Animals, Genetically Modified</classCode> <classCode scheme="mesh">Immunohistochemistry</classCode> <classCode scheme="mesh">In Situ Hybridization</classCode> <classCode scheme="mesh">Mesencephalon</classCode> <classCode scheme="mesh">Receptors, Fibroblast Growth Factor</classCode> <classCode scheme="mesh">Rhombencephalon</classCode> <classCode scheme="mesh">Signal Transduction</classCode> <classCode scheme="mesh">Zebrafish</classCode> <classCode scheme="mesh">Brain</classCode> <classCode scheme="mesh">Cell Division</classCode> <classCode scheme="mesh">Cerebral Ventricles</classCode> <classCode scheme="mesh">Fibroblast Growth Factors</classCode> <classCode scheme="mesh">Genes, Reporter</classCode> <classCode scheme="mesh">Glial Fibrillary Acidic Protein</classCode> <classCode scheme="mesh">Green Fluorescent Proteins</classCode> <classCode scheme="mesh">Hypothalamus</classCode> <classCode scheme="halDomain" n="sdv.neu">Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]</classCode> <classCode scheme="halTypology" n="ART">Journal articles</classCode> </textClass> <abstract xml:lang="en"> <p>The zebrafish adult brain contains numerous neural progenitors and is a good model to approach the general mechanisms of adult neural stem cell maintenance and neurogenesis. Here we use this model to test for a correlation between Fgf signaling and cell proliferation in adult progenitor zones. We report expression of Fgf signals (fgf3,4,8a,8b,17b), receptors (fgfr1-4), and targets (erm, pea3, dusp6, spry1,2,4, and P-ERK) and document that genes of the embryonic fgf8 synexpression group acquire strikingly divergent patterns in the adult brain. We further document the specific expression of fgf3, fgfr1-3, dusp6, and P-ERK in ventricular zones, which contain neural progenitors. In these locations, however, a comparison at the single-cell level of fgfr/P-ERK expression with bromo-deoxy-uridine (BrdU) incorporation and the proliferation marker MCM5 indicates that Fgf signaling is not specifically associated with proliferating progenitors. Rather, it correlates with the ventricular radial glia state, some of which only are progenitors. Together these results stress the importance of Fgf signaling in the adult brain and establish the basis to study its function in zebrafish, in particular in relation to adult neurogenesis.</p> </abstract> </profileDesc> </hal></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Hal/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000152 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Hal/Checkpoint/biblio.hfd -nk 000152 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Hal
|étape= Checkpoint
|type= RBID
|clé= Hal:hal-01055260
|texte= Fgf signaling in the zebrafish adult brain: association of Fgf activity with ventricular zones but not cell proliferation.
}}
| This area was generated with Dilib version V0.6.33. |  |