Modified tetrazolium-based colorimetric method for determining the activities of anti-HIV compounds.
Identifieur interne : 000858 ( PubMed/Checkpoint ); précédent : 000857; suivant : 000859Modified tetrazolium-based colorimetric method for determining the activities of anti-HIV compounds.
Auteurs : N K Ayisi [Ghana] ; S V Gupta ; L F QualtiereSource :
- Journal of virological methods [ 0166-0934 ] ; 1991.
Descripteurs français
- KwdFr :
- Acide phosphono-acétique (analogues et dérivés), Acide phosphono-acétique (pharmacologie), Antiviraux (pharmacologie), Colorimétrie (), Didéoxyinosine (pharmacologie), Foscarnet, Humains, Lignée cellulaire, Sels de tétrazolium, Thymidine (pharmacologie), VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (), Zalcitabine (pharmacologie), Zidovudine (pharmacologie).
- MESH :
- analogues et dérivés : Acide phosphono-acétique.
- pharmacologie : Acide phosphono-acétique, Antiviraux, Didéoxyinosine, Thymidine, Zalcitabine, Zidovudine.
- Colorimétrie, Foscarnet, Humains, Lignée cellulaire, Sels de tétrazolium, VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
English descriptors
- KwdEn :
- Antiviral Agents (pharmacology), Cell Line, Colorimetry (methods), Didanosine (pharmacology), Foscarnet, HIV-1 (drug effects), Humans, Phosphonoacetic Acid (analogs & derivatives), Phosphonoacetic Acid (pharmacology), Tetrazolium Salts, Thymidine (pharmacology), Zalcitabine (pharmacology), Zidovudine (pharmacology).
- MESH :
- chemical , analogs & derivatives : Phosphonoacetic Acid.
- chemical , pharmacology : Antiviral Agents, Didanosine, Phosphonoacetic Acid, Thymidine, Zalcitabine, Zidovudine.
- drug effects : HIV-1.
- methods : Colorimetry.
- Cell Line, Foscarnet, Humans, Tetrazolium Salts.
Abstract
A modified tetrazolium-based colorimetric assay was used to determine the anti-HIV activities of ddAzThd, ddCyd, ddIno, and PFA. In this assay, poly-1-lysine-coated plates were used to attach the MT-2 cells to the bottom of the plates. A fixed amount of virus (50 TCID50) was used in each well. A modified version of the formula published by Pauwels et al. (1988) was used for calculating the percentage cell protection from virus infection. Using CC10/EC90 to calculate the selective indices, the decreasing order of selectivity against HIV-1 strain A87SF, was: ddAzThd greater than PFA greater than ddCyd greater than ddIno. Against HIV-1 strain A79SK-1 the decreasing order of selectivity was: PFA greater than ddIno greater than AzThd greater than ddCyd. The modified formula showed lack of anti-HIV activity for thymidine at non-toxic concentrations.
PubMed: 1664434
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Legon.</nlm:affiliation><orgName type="university">Université du Ghana</orgName><country>Ghana</country><placeName><settlement type="city">Accra</settlement><settlement type="town">Legon (Ghana)</settlement><region type="region">Région du Grand Accra</region></placeName></affiliation></author><author><name sortKey="Gupta, S V" sort="Gupta, S V" uniqKey="Gupta S" first="S V" last="Gupta">S V Gupta</name></author><author><name sortKey="Qualtiere, L F" sort="Qualtiere, L F" uniqKey="Qualtiere L" first="L F" last="Qualtiere">L F Qualtiere</name></author></analytic><series><title level="j">Journal of virological methods</title><idno type="ISSN">0166-0934</idno><imprint><date when="1991" type="published">1991</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antiviral Agents (pharmacology)</term><term>Cell Line</term><term>Colorimetry (methods)</term><term>Didanosine (pharmacology)</term><term>Foscarnet</term><term>HIV-1 (drug effects)</term><term>Humans</term><term>Phosphonoacetic Acid (analogs & derivatives)</term><term>Phosphonoacetic Acid (pharmacology)</term><term>Tetrazolium Salts</term><term>Thymidine (pharmacology)</term><term>Zalcitabine (pharmacology)</term><term>Zidovudine (pharmacology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acide phosphono-acétique (analogues et dérivés)</term><term>Acide phosphono-acétique (pharmacologie)</term><term>Antiviraux (pharmacologie)</term><term>Colorimétrie ()</term><term>Didéoxyinosine (pharmacologie)</term><term>Foscarnet</term><term>Humains</term><term>Lignée cellulaire</term><term>Sels de tétrazolium</term><term>Thymidine (pharmacologie)</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) ()</term><term>Zalcitabine (pharmacologie)</term><term>Zidovudine (pharmacologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Phosphonoacetic Acid</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antiviral Agents</term><term>Didanosine</term><term>Phosphonoacetic Acid</term><term>Thymidine</term><term>Zalcitabine</term><term>Zidovudine</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Acide phosphono-acétique</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>HIV-1</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Colorimetry</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Acide phosphono-acétique</term><term>Antiviraux</term><term>Didéoxyinosine</term><term>Thymidine</term><term>Zalcitabine</term><term>Zidovudine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Line</term><term>Foscarnet</term><term>Humans</term><term>Tetrazolium Salts</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Colorimétrie</term><term>Foscarnet</term><term>Humains</term><term>Lignée cellulaire</term><term>Sels de tétrazolium</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A modified tetrazolium-based colorimetric assay was used to determine the anti-HIV activities of ddAzThd, ddCyd, ddIno, and PFA. In this assay, poly-1-lysine-coated plates were used to attach the MT-2 cells to the bottom of the plates. A fixed amount of virus (50 TCID50) was used in each well. A modified version of the formula published by Pauwels et al. (1988) was used for calculating the percentage cell protection from virus infection. Using CC10/EC90 to calculate the selective indices, the decreasing order of selectivity against HIV-1 strain A87SF, was: ddAzThd greater than PFA greater than ddCyd greater than ddIno. Against HIV-1 strain A79SK-1 the decreasing order of selectivity was: PFA greater than ddIno greater than AzThd greater than ddCyd. The modified formula showed lack of anti-HIV activity for thymidine at non-toxic concentrations.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">1664434</PMID><DateCreated><Year>1992</Year><Month>03</Month><Day>19</Day></DateCreated><DateCompleted><Year>1992</Year><Month>03</Month><Day>19</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0166-0934</ISSN><JournalIssue CitedMedium="Print"><Volume>33</Volume><Issue>3</Issue><PubDate><Year>1991</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Journal of virological methods</Title><ISOAbbreviation>J. Virol. Methods</ISOAbbreviation></Journal><ArticleTitle>Modified tetrazolium-based colorimetric method for determining the activities of anti-HIV compounds.</ArticleTitle><Pagination><MedlinePgn>335-44</MedlinePgn></Pagination><Abstract><AbstractText>A modified tetrazolium-based colorimetric assay was used to determine the anti-HIV activities of ddAzThd, ddCyd, ddIno, and PFA. In this assay, poly-1-lysine-coated plates were used to attach the MT-2 cells to the bottom of the plates. A fixed amount of virus (50 TCID50) was used in each well. A modified version of the formula published by Pauwels et al. (1988) was used for calculating the percentage cell protection from virus infection. Using CC10/EC90 to calculate the selective indices, the decreasing order of selectivity against HIV-1 strain A87SF, was: ddAzThd greater than PFA greater than ddCyd greater than ddIno. Against HIV-1 strain A79SK-1 the decreasing order of selectivity was: PFA greater than ddIno greater than AzThd greater than ddCyd. The modified formula showed lack of anti-HIV activity for thymidine at non-toxic concentrations.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ayisi</LastName><ForeName>N K</ForeName><Initials>NK</Initials><AffiliationInfo><Affiliation>Noguchi Memorial Institute for Medical Research, University of Ghana, Legon.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gupta</LastName><ForeName>S V</ForeName><Initials>SV</Initials></Author><Author ValidYN="Y"><LastName>Qualtiere</LastName><ForeName>L F</ForeName><Initials>LF</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Virol Methods</MedlineTA><NlmUniqueID>8005839</NlmUniqueID><ISSNLinking>0166-0934</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000998">Antiviral Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013778">Tetrazolium Salts</NameOfSubstance></Chemical><Chemical><RegistryNumber>364P9RVW4X</RegistryNumber><NameOfSubstance UI="D017245">Foscarnet</NameOfSubstance></Chemical><Chemical><RegistryNumber>4B9XT59T7S</RegistryNumber><NameOfSubstance UI="D015215">Zidovudine</NameOfSubstance></Chemical><Chemical><RegistryNumber>6L3XT8CB3I</RegistryNumber><NameOfSubstance UI="D016047">Zalcitabine</NameOfSubstance></Chemical><Chemical><RegistryNumber>K3GDH6OH08</RegistryNumber><NameOfSubstance UI="D016049">Didanosine</NameOfSubstance></Chemical><Chemical><RegistryNumber>N919E46723</RegistryNumber><NameOfSubstance UI="D010746">Phosphonoacetic 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MajorTopicYN="N">Foscarnet</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015497" MajorTopicYN="N">HIV-1</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010746" MajorTopicYN="N">Phosphonoacetic Acid</DescriptorName><QualifierName UI="Q000031" MajorTopicYN="N">analogs & derivatives</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013778" MajorTopicYN="Y">Tetrazolium Salts</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013936" MajorTopicYN="N">Thymidine</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016047" MajorTopicYN="N">Zalcitabine</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015215" MajorTopicYN="N">Zidovudine</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1991</Year><Month>8</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1991</Year><Month>8</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1991</Year><Month>8</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">1664434</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Ghana</li></country><region><li>Région du Grand Accra</li></region><settlement><li>Accra</li><li>Legon (Ghana)</li></settlement><orgName><li>Université du Ghana</li></orgName></list><tree><noCountry><name sortKey="Gupta, S V" sort="Gupta, S V" uniqKey="Gupta S" first="S V" last="Gupta">S V Gupta</name><name sortKey="Qualtiere, L F" sort="Qualtiere, L F" uniqKey="Qualtiere L" first="L F" last="Qualtiere">L F Qualtiere</name></noCountry><country name="Ghana"><region name="Région du Grand Accra"><name sortKey="Ayisi, N K" sort="Ayisi, N K" uniqKey="Ayisi N" first="N K" last="Ayisi">N K Ayisi</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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