Modified tetrazolium-based colorimetric method for determining the activities of anti-HIV compounds.
Identifieur interne : 000876 ( PubMed/Corpus ); précédent : 000875; suivant : 000877Modified tetrazolium-based colorimetric method for determining the activities of anti-HIV compounds.
Auteurs : N K Ayisi ; S V Gupta ; L F QualtiereSource :
- Journal of virological methods [ 0166-0934 ] ; 1991.
English descriptors
- KwdEn :
- Antiviral Agents (pharmacology), Cell Line, Colorimetry (methods), Didanosine (pharmacology), Foscarnet, HIV-1 (drug effects), Humans, Phosphonoacetic Acid (analogs & derivatives), Phosphonoacetic Acid (pharmacology), Tetrazolium Salts, Thymidine (pharmacology), Zalcitabine (pharmacology), Zidovudine (pharmacology).
- MESH :
- chemical , analogs & derivatives : Phosphonoacetic Acid.
- chemical , pharmacology : Antiviral Agents, Didanosine, Phosphonoacetic Acid, Thymidine, Zalcitabine, Zidovudine.
- drug effects : HIV-1.
- methods : Colorimetry.
- Cell Line, Foscarnet, Humans, Tetrazolium Salts.
Abstract
A modified tetrazolium-based colorimetric assay was used to determine the anti-HIV activities of ddAzThd, ddCyd, ddIno, and PFA. In this assay, poly-1-lysine-coated plates were used to attach the MT-2 cells to the bottom of the plates. A fixed amount of virus (50 TCID50) was used in each well. A modified version of the formula published by Pauwels et al. (1988) was used for calculating the percentage cell protection from virus infection. Using CC10/EC90 to calculate the selective indices, the decreasing order of selectivity against HIV-1 strain A87SF, was: ddAzThd greater than PFA greater than ddCyd greater than ddIno. Against HIV-1 strain A79SK-1 the decreasing order of selectivity was: PFA greater than ddIno greater than AzThd greater than ddCyd. The modified formula showed lack of anti-HIV activity for thymidine at non-toxic concentrations.
PubMed: 1664434
Links to Exploration step
pubmed:1664434Le document en format XML
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<author><name sortKey="Ayisi, N K" sort="Ayisi, N K" uniqKey="Ayisi N" first="N K" last="Ayisi">N K Ayisi</name>
<affiliation><nlm:affiliation>Noguchi Memorial Institute for Medical Research, University of Ghana, Legon.</nlm:affiliation>
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<author><name sortKey="Gupta, S V" sort="Gupta, S V" uniqKey="Gupta S" first="S V" last="Gupta">S V Gupta</name>
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<author><name sortKey="Qualtiere, L F" sort="Qualtiere, L F" uniqKey="Qualtiere L" first="L F" last="Qualtiere">L F Qualtiere</name>
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<author><name sortKey="Ayisi, N K" sort="Ayisi, N K" uniqKey="Ayisi N" first="N K" last="Ayisi">N K Ayisi</name>
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<author><name sortKey="Gupta, S V" sort="Gupta, S V" uniqKey="Gupta S" first="S V" last="Gupta">S V Gupta</name>
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<author><name sortKey="Qualtiere, L F" sort="Qualtiere, L F" uniqKey="Qualtiere L" first="L F" last="Qualtiere">L F Qualtiere</name>
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<series><title level="j">Journal of virological methods</title>
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<imprint><date when="1991" type="published">1991</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antiviral Agents (pharmacology)</term>
<term>Cell Line</term>
<term>Colorimetry (methods)</term>
<term>Didanosine (pharmacology)</term>
<term>Foscarnet</term>
<term>HIV-1 (drug effects)</term>
<term>Humans</term>
<term>Phosphonoacetic Acid (analogs & derivatives)</term>
<term>Phosphonoacetic Acid (pharmacology)</term>
<term>Tetrazolium Salts</term>
<term>Thymidine (pharmacology)</term>
<term>Zalcitabine (pharmacology)</term>
<term>Zidovudine (pharmacology)</term>
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<keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Phosphonoacetic Acid</term>
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<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antiviral Agents</term>
<term>Didanosine</term>
<term>Phosphonoacetic Acid</term>
<term>Thymidine</term>
<term>Zalcitabine</term>
<term>Zidovudine</term>
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<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>HIV-1</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Colorimetry</term>
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<keywords scheme="MESH" xml:lang="en"><term>Cell Line</term>
<term>Foscarnet</term>
<term>Humans</term>
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<front><div type="abstract" xml:lang="en">A modified tetrazolium-based colorimetric assay was used to determine the anti-HIV activities of ddAzThd, ddCyd, ddIno, and PFA. In this assay, poly-1-lysine-coated plates were used to attach the MT-2 cells to the bottom of the plates. A fixed amount of virus (50 TCID50) was used in each well. A modified version of the formula published by Pauwels et al. (1988) was used for calculating the percentage cell protection from virus infection. Using CC10/EC90 to calculate the selective indices, the decreasing order of selectivity against HIV-1 strain A87SF, was: ddAzThd greater than PFA greater than ddCyd greater than ddIno. Against HIV-1 strain A79SK-1 the decreasing order of selectivity was: PFA greater than ddIno greater than AzThd greater than ddCyd. The modified formula showed lack of anti-HIV activity for thymidine at non-toxic concentrations.</div>
</front>
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<Title>Journal of virological methods</Title>
<ISOAbbreviation>J. Virol. Methods</ISOAbbreviation>
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<ArticleTitle>Modified tetrazolium-based colorimetric method for determining the activities of anti-HIV compounds.</ArticleTitle>
<Pagination><MedlinePgn>335-44</MedlinePgn>
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<Abstract><AbstractText>A modified tetrazolium-based colorimetric assay was used to determine the anti-HIV activities of ddAzThd, ddCyd, ddIno, and PFA. In this assay, poly-1-lysine-coated plates were used to attach the MT-2 cells to the bottom of the plates. A fixed amount of virus (50 TCID50) was used in each well. A modified version of the formula published by Pauwels et al. (1988) was used for calculating the percentage cell protection from virus infection. Using CC10/EC90 to calculate the selective indices, the decreasing order of selectivity against HIV-1 strain A87SF, was: ddAzThd greater than PFA greater than ddCyd greater than ddIno. Against HIV-1 strain A79SK-1 the decreasing order of selectivity was: PFA greater than ddIno greater than AzThd greater than ddCyd. The modified formula showed lack of anti-HIV activity for thymidine at non-toxic concentrations.</AbstractText>
</Abstract>
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<MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName>
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