Sero-diagnosis of tuberculosis with A60 antigen enzyme-linked immunosorbent assay: failure in HIV-infected individuals in Ghana.
Identifieur interne : 000139 ( Ncbi/Curation ); précédent : 000138; suivant : 000140Sero-diagnosis of tuberculosis with A60 antigen enzyme-linked immunosorbent assay: failure in HIV-infected individuals in Ghana.
Auteurs : T S Van Der Werf [Ghana] ; P K Das ; D. Van Soolingen ; S. Yong ; T W Van Der Mark ; R. Van Den AkkerSource :
- Medical microbiology and immunology [ 0300-8584 ] ; 1992.
Descripteurs français
- KwdFr :
- Adulte, Anticorps anti-VIH (sang), Anticorps antibactériens (sang), Antigènes bactériens, Erreurs de diagnostic, Humains, Immunoglobuline G (sang), Infections à VIH (), Infections à VIH (immunologie), Sensibilité et spécificité, Test ELISA (), Tuberculose pulmonaire (), Tuberculose pulmonaire (diagnostic), Tuberculose pulmonaire (immunologie), VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (immunologie), VIH-2 (Virus de l'Immunodéficience Humaine de type 2) (immunologie).
- MESH :
- diagnostic : Tuberculose pulmonaire.
- immunologie : Infections à VIH, Tuberculose pulmonaire, VIH-1 (Virus de l'Immunodéficience Humaine de type 1), VIH-2 (Virus de l'Immunodéficience Humaine de type 2).
- sang : Anticorps anti-VIH, Anticorps antibactériens, Immunoglobuline G.
- Adulte, Antigènes bactériens, Erreurs de diagnostic, Humains, Infections à VIH, Sensibilité et spécificité, Test ELISA, Tuberculose pulmonaire.
English descriptors
- KwdEn :
- Adult, Antibodies, Bacterial (blood), Antigens, Bacterial, Diagnostic Errors, Enzyme-Linked Immunosorbent Assay (methods), Enzyme-Linked Immunosorbent Assay (statistics & numerical data), HIV Antibodies (blood), HIV Infections (complications), HIV Infections (immunology), HIV-1 (immunology), HIV-2 (immunology), Humans, Immunoglobulin G (blood), Sensitivity and Specificity, Tuberculosis, Pulmonary (complications), Tuberculosis, Pulmonary (diagnosis), Tuberculosis, Pulmonary (immunology).
- MESH :
- chemical , blood : Antibodies, Bacterial, HIV Antibodies, Immunoglobulin G.
- complications : HIV Infections, Tuberculosis, Pulmonary.
- diagnosis : Tuberculosis, Pulmonary.
- immunology : HIV Infections, HIV-1, HIV-2, Tuberculosis, Pulmonary.
- methods : Enzyme-Linked Immunosorbent Assay.
- statistics & numerical data : Enzyme-Linked Immunosorbent Assay.
- Adult, Antigens, Bacterial, Diagnostic Errors, Humans, Sensitivity and Specificity.
Abstract
In order to assess the diagnostic usefulness of the A60 (ANDA Biological, Strassbourg, France) sero-diagnostic enzyme-linked immunosorbent assay (ELISA) kit for tuberculosis in Africa, sera of 53 pulmonary smear-positive tuberculosis (TB) patients, 30 apparently healthy control subjects and 6 AIDS suspects were sampled in Agogo Hospital in the forest area of Ghana. These sera were analyzed for antibodies to HIV-1 and HIV-2, and IgG-antibodies to the A60 BCG-antigen, while the non-HIV individuals were tested for total IgG levels. One healthy control subject, all of 6 AIDS suspects and 7 of the TB patients has HIV infections. In the non-HIV TB group, the sensitivity and specifity of the A60 ELISA was 78% and 86%, respectively, which was much poorer than expected from published reports about the A60 test. The A60 test failed, completely however, to discriminate between TB and non-TB in the HIV-positive group. In the non-HIV groups, total IgG levels were significantly higher in TB patients than in controls. It seems that the usefulness of the A60 ELISA test to diagnose tuberculosis is very limited in this high-incidence area, and that it seems to be of no value in patients infected with HIV.
PubMed: 1406459
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Yong</name></author><author><name sortKey="Van Der Mark, T W" sort="Van Der Mark, T W" uniqKey="Van Der Mark T" first="T W" last="Van Der Mark">T W Van Der Mark</name></author><author><name sortKey="Van Den Akker, R" sort="Van Den Akker, R" uniqKey="Van Den Akker R" first="R" last="Van Den Akker">R. 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Van Soolingen</name></author><author><name sortKey="Yong, S" sort="Yong, S" uniqKey="Yong S" first="S" last="Yong">S. Yong</name></author><author><name sortKey="Van Der Mark, T W" sort="Van Der Mark, T W" uniqKey="Van Der Mark T" first="T W" last="Van Der Mark">T W Van Der Mark</name></author><author><name sortKey="Van Den Akker, R" sort="Van Den Akker, R" uniqKey="Van Den Akker R" first="R" last="Van Den Akker">R. Van Den Akker</name></author></analytic><series><title level="j">Medical microbiology and immunology</title><idno type="ISSN">0300-8584</idno><imprint><date when="1992" type="published">1992</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Antibodies, Bacterial (blood)</term><term>Antigens, Bacterial</term><term>Diagnostic Errors</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Enzyme-Linked Immunosorbent Assay (statistics & numerical data)</term><term>HIV Antibodies (blood)</term><term>HIV Infections (complications)</term><term>HIV Infections (immunology)</term><term>HIV-1 (immunology)</term><term>HIV-2 (immunology)</term><term>Humans</term><term>Immunoglobulin G (blood)</term><term>Sensitivity and Specificity</term><term>Tuberculosis, Pulmonary (complications)</term><term>Tuberculosis, Pulmonary (diagnosis)</term><term>Tuberculosis, Pulmonary (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Anticorps anti-VIH (sang)</term><term>Anticorps antibactériens (sang)</term><term>Antigènes bactériens</term><term>Erreurs de diagnostic</term><term>Humains</term><term>Immunoglobuline G (sang)</term><term>Infections à VIH ()</term><term>Infections à VIH (immunologie)</term><term>Sensibilité et spécificité</term><term>Test ELISA ()</term><term>Tuberculose pulmonaire ()</term><term>Tuberculose pulmonaire (diagnostic)</term><term>Tuberculose pulmonaire (immunologie)</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (immunologie)</term><term>VIH-2 (Virus de l'Immunodéficience Humaine de type 2) (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Bacterial</term><term>HIV Antibodies</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" qualifier="complications" xml:lang="en"><term>HIV Infections</term><term>Tuberculosis, Pulmonary</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Tuberculosis, Pulmonary</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Tuberculose pulmonaire</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Infections à VIH</term><term>Tuberculose pulmonaire</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term><term>VIH-2 (Virus de l'Immunodéficience Humaine de type 2)</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>HIV Infections</term><term>HIV-1</term><term>HIV-2</term><term>Tuberculosis, Pulmonary</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps anti-VIH</term><term>Anticorps antibactériens</term><term>Immunoglobuline G</term></keywords><keywords scheme="MESH" qualifier="statistics & numerical data" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Antigens, Bacterial</term><term>Diagnostic Errors</term><term>Humans</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Antigènes bactériens</term><term>Erreurs de diagnostic</term><term>Humains</term><term>Infections à VIH</term><term>Sensibilité et spécificité</term><term>Test ELISA</term><term>Tuberculose pulmonaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In order to assess the diagnostic usefulness of the A60 (ANDA Biological, Strassbourg, France) sero-diagnostic enzyme-linked immunosorbent assay (ELISA) kit for tuberculosis in Africa, sera of 53 pulmonary smear-positive tuberculosis (TB) patients, 30 apparently healthy control subjects and 6 AIDS suspects were sampled in Agogo Hospital in the forest area of Ghana. These sera were analyzed for antibodies to HIV-1 and HIV-2, and IgG-antibodies to the A60 BCG-antigen, while the non-HIV individuals were tested for total IgG levels. One healthy control subject, all of 6 AIDS suspects and 7 of the TB patients has HIV infections. In the non-HIV TB group, the sensitivity and specifity of the A60 ELISA was 78% and 86%, respectively, which was much poorer than expected from published reports about the A60 test. The A60 test failed, completely however, to discriminate between TB and non-TB in the HIV-positive group. In the non-HIV groups, total IgG levels were significantly higher in TB patients than in controls. It seems that the usefulness of the A60 ELISA test to diagnose tuberculosis is very limited in this high-incidence area, and that it seems to be of no value in patients infected with HIV.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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