Infectious DNA clone of HIV type 1 A/G recombinant (CRF02_AG) replicable in peripheral blood mononuclear cells.
Identifieur interne : 000042 ( Ncbi/Curation ); précédent : 000041; suivant : 000043Infectious DNA clone of HIV type 1 A/G recombinant (CRF02_AG) replicable in peripheral blood mononuclear cells.
Auteurs : M. Takahoko [Japon] ; M. Tobiume ; K. Ishikawa ; W. Ampofo ; N. Yamamoto ; M. Matsuda ; M. TatsumiSource :
- AIDS research and human retroviruses [ 0889-2229 ] ; 2001.
Descripteurs français
- KwdFr :
- ADN recombiné (génétique), ADN recombiné (isolement et purification), Adulte, Agranulocytes (virologie), Analyse de séquence d'ADN, Cellules cancéreuses en culture, Clonage moléculaire, Données de séquences moléculaires, Humains, Infections à VIH (génétique), Infections à VIH (transmission), Infections à VIH (virologie), Mâle, Phylogénie, Réplication virale, Transfection, VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique).
- MESH :
- génétique : ADN recombiné, Infections à VIH, VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
- isolement et purification : ADN recombiné.
- virologie : Agranulocytes, Infections à VIH.
- transmission : Adulte, Analyse de séquence d'ADN, Cellules cancéreuses en culture, Clonage moléculaire, Données de séquences moléculaires, Humains, Infections à VIH, Mâle, Phylogénie, Réplication virale, Transfection.
English descriptors
- KwdEn :
- Adult, Cloning, Molecular, DNA, Recombinant (genetics), DNA, Recombinant (isolation & purification), HIV Infections (genetics), HIV Infections (transmission), HIV Infections (virology), HIV-1 (genetics), Humans, Leukocytes, Mononuclear (virology), Male, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Transfection, Tumor Cells, Cultured, Virus Replication.
- MESH :
- chemical , genetics : DNA, Recombinant.
- chemical , isolation & purification : DNA, Recombinant.
- genetics : HIV Infections, HIV-1.
- transmission : HIV Infections.
- virology : HIV Infections, Leukocytes, Mononuclear.
- Adult, Cloning, Molecular, Humans, Male, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Transfection, Tumor Cells, Cultured, Virus Replication.
Abstract
We constructed an infectious DNA clone of the HIV-1 A/G recombinant 97GH-AG2, which was isolated in Ghana in 1997 and was classified originally as subtype A. By phylogenetic and recombination breakpoint analyses, p97GH-AG2 was grouped in the circulating form of A/G recombinants (CRF02_AG) and was found to contain the least amount of subtype G-derived region among the known CRF02_AG HIV-1 DNAs. This result suggests that CRF02_AG may be a predominant form in Ghana. Virions produced by transfection of p97GH-AG2 into 293T cells grew in phytohemagglutinin-stimulated peripheral blood mononuclear cells (PBMCs). 97GH-AG2 also replicated efficiently in CCR5-expressing HeLa cells, MAGIC5, but only weakly in the parent MAGI cells, indicating that 97GH-AG2 uses mostly CCR5 as a coreceptor. Isolation of the first HIV-1 (CRF02_AG) DNA clone that replicates in PBMCs will accelerate the molecular analysis of this subtype.
DOI: 10.1089/088922201300343771
PubMed: 11485626
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pubmed:11485626Le document en format XML
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Tobiume</name></author><author><name sortKey="Ishikawa, K" sort="Ishikawa, K" uniqKey="Ishikawa K" first="K" last="Ishikawa">K. Ishikawa</name></author><author><name sortKey="Ampofo, W" sort="Ampofo, W" uniqKey="Ampofo W" first="W" last="Ampofo">W. Ampofo</name></author><author><name sortKey="Yamamoto, N" sort="Yamamoto, N" uniqKey="Yamamoto N" first="N" last="Yamamoto">N. Yamamoto</name></author><author><name sortKey="Matsuda, M" sort="Matsuda, M" uniqKey="Matsuda M" first="M" last="Matsuda">M. Matsuda</name></author><author><name sortKey="Tatsumi, M" sort="Tatsumi, M" uniqKey="Tatsumi M" first="M" last="Tatsumi">M. Tatsumi</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2001">2001</date><idno type="RBID">pubmed:11485626</idno><idno type="pmid">11485626</idno><idno type="doi">10.1089/088922201300343771</idno><idno type="wicri:Area/PubMed/Corpus">000661</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000661</idno><idno type="wicri:Area/PubMed/Curation">000661</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000661</idno><idno type="wicri:Area/PubMed/Checkpoint">000661</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000661</idno><idno type="wicri:Area/Ncbi/Merge">000042</idno><idno type="wicri:Area/Ncbi/Curation">000042</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Infectious DNA clone of HIV type 1 A/G recombinant (CRF02_AG) replicable in peripheral blood mononuclear cells.</title><author><name sortKey="Takahoko, M" sort="Takahoko, M" uniqKey="Takahoko M" first="M" last="Takahoko">M. Takahoko</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathology, Research Institute, International Medical Center of Japan, Tokyo 162-8644, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, Research Institute, International Medical Center of Japan, Tokyo 162-8644</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Tobiume, M" sort="Tobiume, M" uniqKey="Tobiume M" first="M" last="Tobiume">M. Tobiume</name></author><author><name sortKey="Ishikawa, K" sort="Ishikawa, K" uniqKey="Ishikawa K" first="K" last="Ishikawa">K. Ishikawa</name></author><author><name sortKey="Ampofo, W" sort="Ampofo, W" uniqKey="Ampofo W" first="W" last="Ampofo">W. Ampofo</name></author><author><name sortKey="Yamamoto, N" sort="Yamamoto, N" uniqKey="Yamamoto N" first="N" last="Yamamoto">N. Yamamoto</name></author><author><name sortKey="Matsuda, M" sort="Matsuda, M" uniqKey="Matsuda M" first="M" last="Matsuda">M. Matsuda</name></author><author><name sortKey="Tatsumi, M" sort="Tatsumi, M" uniqKey="Tatsumi M" first="M" last="Tatsumi">M. Tatsumi</name></author></analytic><series><title level="j">AIDS research and human retroviruses</title><idno type="ISSN">0889-2229</idno><imprint><date when="2001" type="published">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Cloning, Molecular</term><term>DNA, Recombinant (genetics)</term><term>DNA, Recombinant (isolation & purification)</term><term>HIV Infections (genetics)</term><term>HIV Infections (transmission)</term><term>HIV Infections (virology)</term><term>HIV-1 (genetics)</term><term>Humans</term><term>Leukocytes, Mononuclear (virology)</term><term>Male</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term><term>Transfection</term><term>Tumor Cells, Cultured</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN recombiné (génétique)</term><term>ADN recombiné (isolement et purification)</term><term>Adulte</term><term>Agranulocytes (virologie)</term><term>Analyse de séquence d'ADN</term><term>Cellules cancéreuses en culture</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Infections à VIH (génétique)</term><term>Infections à VIH (transmission)</term><term>Infections à VIH (virologie)</term><term>Mâle</term><term>Phylogénie</term><term>Réplication virale</term><term>Transfection</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Recombinant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA, Recombinant</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>HIV Infections</term><term>HIV-1</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN recombiné</term><term>Infections à VIH</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ADN recombiné</term></keywords><keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>HIV Infections</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Agranulocytes</term><term>Infections à VIH</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>HIV Infections</term><term>Leukocytes, Mononuclear</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Cloning, Molecular</term><term>Humans</term><term>Male</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term><term>Transfection</term><term>Tumor Cells, Cultured</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="transmission" xml:lang="fr"><term>Adulte</term><term>Analyse de séquence d'ADN</term><term>Cellules cancéreuses en culture</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Infections à VIH</term><term>Mâle</term><term>Phylogénie</term><term>Réplication virale</term><term>Transfection</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We constructed an infectious DNA clone of the HIV-1 A/G recombinant 97GH-AG2, which was isolated in Ghana in 1997 and was classified originally as subtype A. By phylogenetic and recombination breakpoint analyses, p97GH-AG2 was grouped in the circulating form of A/G recombinants (CRF02_AG) and was found to contain the least amount of subtype G-derived region among the known CRF02_AG HIV-1 DNAs. This result suggests that CRF02_AG may be a predominant form in Ghana. Virions produced by transfection of p97GH-AG2 into 293T cells grew in phytohemagglutinin-stimulated peripheral blood mononuclear cells (PBMCs). 97GH-AG2 also replicated efficiently in CCR5-expressing HeLa cells, MAGIC5, but only weakly in the parent MAGI cells, indicating that 97GH-AG2 uses mostly CCR5 as a coreceptor. Isolation of the first HIV-1 (CRF02_AG) DNA clone that replicates in PBMCs will accelerate the molecular analysis of this subtype.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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