Infectious DNA clone of HIV type 1 A/G recombinant (CRF02_AG) replicable in peripheral blood mononuclear cells.
Identifieur interne : 000042 ( Ncbi/Merge ); précédent : 000041; suivant : 000043Infectious DNA clone of HIV type 1 A/G recombinant (CRF02_AG) replicable in peripheral blood mononuclear cells.
Auteurs : M. Takahoko [Japon] ; M. Tobiume ; K. Ishikawa ; W. Ampofo ; N. Yamamoto ; M. Matsuda ; M. TatsumiSource :
- AIDS research and human retroviruses [ 0889-2229 ] ; 2001.
Descripteurs français
- KwdFr :
- ADN recombiné (génétique), ADN recombiné (isolement et purification), Adulte, Agranulocytes (virologie), Analyse de séquence d'ADN, Cellules cancéreuses en culture, Clonage moléculaire, Données de séquences moléculaires, Humains, Infections à VIH (génétique), Infections à VIH (transmission), Infections à VIH (virologie), Mâle, Phylogénie, Réplication virale, Transfection, VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique).
- MESH :
- génétique : ADN recombiné, Infections à VIH, VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
- isolement et purification : ADN recombiné.
- virologie : Agranulocytes, Infections à VIH.
- transmission : Adulte, Analyse de séquence d'ADN, Cellules cancéreuses en culture, Clonage moléculaire, Données de séquences moléculaires, Humains, Infections à VIH, Mâle, Phylogénie, Réplication virale, Transfection.
English descriptors
- KwdEn :
- Adult, Cloning, Molecular, DNA, Recombinant (genetics), DNA, Recombinant (isolation & purification), HIV Infections (genetics), HIV Infections (transmission), HIV Infections (virology), HIV-1 (genetics), Humans, Leukocytes, Mononuclear (virology), Male, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Transfection, Tumor Cells, Cultured, Virus Replication.
- MESH :
- chemical , genetics : DNA, Recombinant.
- chemical , isolation & purification : DNA, Recombinant.
- genetics : HIV Infections, HIV-1.
- transmission : HIV Infections.
- virology : HIV Infections, Leukocytes, Mononuclear.
- Adult, Cloning, Molecular, Humans, Male, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Transfection, Tumor Cells, Cultured, Virus Replication.
Abstract
We constructed an infectious DNA clone of the HIV-1 A/G recombinant 97GH-AG2, which was isolated in Ghana in 1997 and was classified originally as subtype A. By phylogenetic and recombination breakpoint analyses, p97GH-AG2 was grouped in the circulating form of A/G recombinants (CRF02_AG) and was found to contain the least amount of subtype G-derived region among the known CRF02_AG HIV-1 DNAs. This result suggests that CRF02_AG may be a predominant form in Ghana. Virions produced by transfection of p97GH-AG2 into 293T cells grew in phytohemagglutinin-stimulated peripheral blood mononuclear cells (PBMCs). 97GH-AG2 also replicated efficiently in CCR5-expressing HeLa cells, MAGIC5, but only weakly in the parent MAGI cells, indicating that 97GH-AG2 uses mostly CCR5 as a coreceptor. Isolation of the first HIV-1 (CRF02_AG) DNA clone that replicates in PBMCs will accelerate the molecular analysis of this subtype.
DOI: 10.1089/088922201300343771
PubMed: 11485626
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000661
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pubmed:11485626Le document en format XML
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By phylogenetic and recombination breakpoint analyses, p97GH-AG2 was grouped in the circulating form of A/G recombinants (CRF02_AG) and was found to contain the least amount of subtype G-derived region among the known CRF02_AG HIV-1 DNAs. This result suggests that CRF02_AG may be a predominant form in Ghana. Virions produced by transfection of p97GH-AG2 into 293T cells grew in phytohemagglutinin-stimulated peripheral blood mononuclear cells (PBMCs). 97GH-AG2 also replicated efficiently in CCR5-expressing HeLa cells, MAGIC5, but only weakly in the parent MAGI cells, indicating that 97GH-AG2 uses mostly CCR5 as a coreceptor. Isolation of the first HIV-1 (CRF02_AG) DNA clone that replicates in PBMCs will accelerate the molecular analysis of this subtype.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11485626</PMID><DateCreated><Year>2001</Year><Month>08</Month><Day>03</Day></DateCreated><DateCompleted><Year>2001</Year><Month>10</Month><Day>18</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0889-2229</ISSN><JournalIssue CitedMedium="Print"><Volume>17</Volume><Issue>11</Issue><PubDate><Year>2001</Year><Month>Jul</Month><Day>20</Day></PubDate></JournalIssue><Title>AIDS research and human retroviruses</Title><ISOAbbreviation>AIDS Res. Hum. Retroviruses</ISOAbbreviation></Journal><ArticleTitle>Infectious DNA clone of HIV type 1 A/G recombinant (CRF02_AG) replicable in peripheral blood mononuclear cells.</ArticleTitle><Pagination><MedlinePgn>1083-7</MedlinePgn></Pagination><Abstract><AbstractText>We constructed an infectious DNA clone of the HIV-1 A/G recombinant 97GH-AG2, which was isolated in Ghana in 1997 and was classified originally as subtype A. By phylogenetic and recombination breakpoint analyses, p97GH-AG2 was grouped in the circulating form of A/G recombinants (CRF02_AG) and was found to contain the least amount of subtype G-derived region among the known CRF02_AG HIV-1 DNAs. This result suggests that CRF02_AG may be a predominant form in Ghana. Virions produced by transfection of p97GH-AG2 into 293T cells grew in phytohemagglutinin-stimulated peripheral blood mononuclear cells (PBMCs). 97GH-AG2 also replicated efficiently in CCR5-expressing HeLa cells, MAGIC5, but only weakly in the parent MAGI cells, indicating that 97GH-AG2 uses mostly CCR5 as a coreceptor. Isolation of the first HIV-1 (CRF02_AG) DNA clone that replicates in PBMCs will accelerate the molecular analysis of this subtype.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Takahoko</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Pathology, Research Institute, International Medical Center of Japan, Tokyo 162-8644, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tobiume</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Ishikawa</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Ampofo</LastName><ForeName>W</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Yamamoto</LastName><ForeName>N</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Matsuda</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Tatsumi</LastName><ForeName>M</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>AB052867</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>AIDS Res Hum Retroviruses</MedlineTA><NlmUniqueID>8709376</NlmUniqueID><ISSNLinking>0889-2229</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004274">DNA, Recombinant</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CitationSubset>X</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004274" MajorTopicYN="N">DNA, Recombinant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015658" MajorTopicYN="N">HIV Infections</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000635" MajorTopicYN="N">transmission</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015497" MajorTopicYN="N">HIV-1</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007963" MajorTopicYN="N">Leukocytes, Mononuclear</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014162" MajorTopicYN="N">Transfection</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014407" MajorTopicYN="N">Tumor Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014779" MajorTopicYN="N">Virus Replication</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2001</Year><Month>8</Month><Day>4</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2001</Year><Month>10</Month><Day>19</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2001</Year><Month>8</Month><Day>4</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11485626</ArticleId><ArticleId IdType="doi">10.1089/088922201300343771</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><noCountry><name sortKey="Ampofo, W" sort="Ampofo, W" uniqKey="Ampofo W" first="W" last="Ampofo">W. Ampofo</name><name sortKey="Ishikawa, K" sort="Ishikawa, K" uniqKey="Ishikawa K" first="K" last="Ishikawa">K. Ishikawa</name><name sortKey="Matsuda, M" sort="Matsuda, M" uniqKey="Matsuda M" first="M" last="Matsuda">M. Matsuda</name><name sortKey="Tatsumi, M" sort="Tatsumi, M" uniqKey="Tatsumi M" first="M" last="Tatsumi">M. Tatsumi</name><name sortKey="Tobiume, M" sort="Tobiume, M" uniqKey="Tobiume M" first="M" last="Tobiume">M. Tobiume</name><name sortKey="Yamamoto, N" sort="Yamamoto, N" uniqKey="Yamamoto N" first="N" last="Yamamoto">N. Yamamoto</name></noCountry><country name="Japon"><region name="Région de Kantō"><name sortKey="Takahoko, M" sort="Takahoko, M" uniqKey="Takahoko M" first="M" last="Takahoko">M. Takahoko</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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