Antibody screening tests variably overestimate the prevalence of hepatitis C virus infection among HIV-infected adults in Ghana.
Identifieur interne : 000393 ( Main/Merge ); précédent : 000392; suivant : 000394Antibody screening tests variably overestimate the prevalence of hepatitis C virus infection among HIV-infected adults in Ghana.
Auteurs : S. King [Royaume-Uni] ; K. Adjei-Asante ; L. Appiah ; D. Adinku ; A. Beloukas ; M. Atkins ; S F Sarfo ; D. Chadwick ; R O Phillips ; A M GerettiSource :
- Journal of viral hepatitis [ 1365-2893 ] ; 2015.
Descripteurs français
- KwdFr :
- ARN viral (sang), Adulte, Adulte d'âge moyen, Analyse de séquence d'ADN, Anticorps de l'hépatite C (sang), Dépistage systématique, Femelle, Ghana (épidémiologie), Génotype, Humains, Hépatite C (diagnostic), Hépatite C (épidémiologie), Infections à VIH (), Mâle, Protéines du core viral (génétique), Protéines virales non structurales (génétique), Techniques de génotypage, Tests sérologiques, Études séroépidémiologiques.
- MESH :
- diagnostic : Hépatite C.
- génétique : Protéines du core viral, Protéines virales non structurales.
- sang : ARN viral, Anticorps de l'hépatite C.
- épidémiologie : Ghana, Hépatite C.
- Adulte, Adulte d'âge moyen, Analyse de séquence d'ADN, Dépistage systématique, Femelle, Génotype, Humains, Infections à VIH, Mâle, Techniques de génotypage, Tests sérologiques, Études séroépidémiologiques.
- Wicri :
- geographic : Ghana.
English descriptors
- KwdEn :
- Adult, Female, Genotype, Genotyping Techniques, Ghana (epidemiology), HIV Infections (complications), Hepatitis C (diagnosis), Hepatitis C (epidemiology), Hepatitis C Antibodies (blood), Humans, Male, Mass Screening, Middle Aged, RNA, Viral (blood), Sequence Analysis, DNA, Seroepidemiologic Studies, Serologic Tests, Viral Core Proteins (genetics), Viral Nonstructural Proteins (genetics).
- MESH :
- chemical , blood : Hepatitis C Antibodies, RNA, Viral.
- geographic , epidemiology : Ghana.
- complications : HIV Infections.
- diagnosis : Hepatitis C.
- epidemiology : Hepatitis C.
- chemical , genetics : Viral Core Proteins, Viral Nonstructural Proteins.
- Adult, Female, Genotype, Genotyping Techniques, Humans, Male, Mass Screening, Middle Aged, Sequence Analysis, DNA, Seroepidemiologic Studies, Serologic Tests.
Abstract
HIV coinfection with HCV has been poorly studied in sub-Saharan Africa, and the reliability of available seroprevalence estimates remains uncertain. The study aim was to determine HCV RNA prevalence in HIV-infected subjects receiving care in Kumasi, Ghana, and relate the findings to HCV antibody detection. From a population of 1520 HIV-infected adults, all HBsAg-positive subjects (n = 236) and a random subset of HBsAg-negative subject (n = 172) were screened for HCV RNA using pooled plasma; positive samples were genotyped by core and NS5B sequencing. HCV antibodies were detected by three commercial screening assays and confirmed by the line immunoassay. HCV RNA was detected in 4/408 subjects (1.0%, 95% confidence interval 0.0-1.9%), comprising 3/236 (1.3%; 0.0-2.8%) HBsAg-positive and 1/172 (0.6%; 0.0-1.8%) HBsAg-negative subjects. HCV RNA-positive subjects showed reactivity in all three antibody screening assays. Among HCV RNA-negative subjects, 5/67 (7.5%), 5/67 (7.5%) and 19/67 (28.4%) showed antibody reactivity by each screening assay, respectively, including two (3.0%) with reactivity by all three assays. Only one sample (1.5%) had confirmed antibody reactivity by line immunoassay indicating past HCV infection. HCV-positive subjects (three males, two females) were aged 30-46 years, by questionnaire-based interview reported surgical procedures and blood transfusion as risk factors for infection. HCV genotypes were 2 (subtypes 2j, 2l, 2k/unassigned) and 1 (subtype unassigned). Without further testing, HCV antibody screening assays variably overestimated HCV prevalence among HIV-infected subjects in Ghana. These findings inform the interpretation of previous seroprevalence estimates based upon screening assays alone.
DOI: 10.1111/jvh.12354
PubMed: 25394987
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pubmed:25394987Le document en format XML
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King</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Infection and Global Health, University of Liverpool, Liverpool, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Institute of Infection and Global Health, University of Liverpool, Liverpool</wicri:regionArea><wicri:noRegion>Liverpool</wicri:noRegion></affiliation></author><author><name sortKey="Adjei Asante, K" sort="Adjei Asante, K" uniqKey="Adjei Asante K" first="K" last="Adjei-Asante">K. Adjei-Asante</name></author><author><name sortKey="Appiah, L" sort="Appiah, L" uniqKey="Appiah L" first="L" last="Appiah">L. Appiah</name></author><author><name sortKey="Adinku, D" sort="Adinku, D" uniqKey="Adinku D" first="D" last="Adinku">D. Adinku</name></author><author><name sortKey="Beloukas, A" sort="Beloukas, A" uniqKey="Beloukas A" first="A" last="Beloukas">A. Beloukas</name></author><author><name sortKey="Atkins, M" sort="Atkins, M" uniqKey="Atkins M" first="M" last="Atkins">M. 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The study aim was to determine HCV RNA prevalence in HIV-infected subjects receiving care in Kumasi, Ghana, and relate the findings to HCV antibody detection. From a population of 1520 HIV-infected adults, all HBsAg-positive subjects (n = 236) and a random subset of HBsAg-negative subject (n = 172) were screened for HCV RNA using pooled plasma; positive samples were genotyped by core and NS5B sequencing. HCV antibodies were detected by three commercial screening assays and confirmed by the line immunoassay. HCV RNA was detected in 4/408 subjects (1.0%, 95% confidence interval 0.0-1.9%), comprising 3/236 (1.3%; 0.0-2.8%) HBsAg-positive and 1/172 (0.6%; 0.0-1.8%) HBsAg-negative subjects. HCV RNA-positive subjects showed reactivity in all three antibody screening assays. Among HCV RNA-negative subjects, 5/67 (7.5%), 5/67 (7.5%) and 19/67 (28.4%) showed antibody reactivity by each screening assay, respectively, including two (3.0%) with reactivity by all three assays. Only one sample (1.5%) had confirmed antibody reactivity by line immunoassay indicating past HCV infection. HCV-positive subjects (three males, two females) were aged 30-46 years, by questionnaire-based interview reported surgical procedures and blood transfusion as risk factors for infection. HCV genotypes were 2 (subtypes 2j, 2l, 2k/unassigned) and 1 (subtype unassigned). Without further testing, HCV antibody screening assays variably overestimated HCV prevalence among HIV-infected subjects in Ghana. These findings inform the interpretation of previous seroprevalence estimates based upon screening assays alone.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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