HBV genotypes and drug resistance mutations in antiretroviral treatment-naïve and treatment-experienced HBV-HIV co-infected patients
Identifieur interne : 000195 ( Main/Merge ); précédent : 000194; suivant : 000196HBV genotypes and drug resistance mutations in antiretroviral treatment-naïve and treatment-experienced HBV-HIV co-infected patients
Auteurs : Timothy N. A. Archampong [Ghana] ; Ceejay L. Boyce [États-Unis] ; Margaret Lartey [Ghana] ; Kwamena W. Sagoe [Ghana] ; Adjoa Obo-Akwa [Ghana] ; Ernest Kenu [Ghana] ; Jason T. Blackard [États-Unis] ; Awewura Kwara [États-Unis]Source :
- Antiviral therapy [ 1359-6535 ] ; 2016.
Abstract
The presence of hepatitis B virus (HBV) resistance mutations upon initiation or during antiretroviral therapy (ART) in HIV co-infected patients is an important determinant of treatment response. The main objective of the study was to determine the prevalence of HBV resistance mutations in antiretroviral treatment-naïve and treatment-experienced HBV-HIV co-infected Ghanaian patients with detectable HBV viremia.
HBV-HIV co-infected patients who were ART-naïve or had received at least 9 months of lamivudine (3TC)-containing ART were enrolled in a cross-sectional study. Demographic and clinical data were collected and HBV DNA quantified. Partial HBV sequences were amplified by PCR and sequenced bi-directionally to obtain a 2.1-2.2 kb fragment for phylogenetic analysis of HBV genotypes and evaluation of drug resistance mutations.
Of the 100 HBV-HIV co-infected study patients, 75 were successfully PCR-amplified, and 63 were successfully sequenced. Of these 63 patients, 27 (42.9%) were ART-experienced, and 58 (92.1%) had HBV genotype E. No resistance mutations were observed in the 36 ART-naïve patients, while 21 (77.8%) of 27 treatment-experienced patients had resistance mutations. All patients with resistance mutations had no tenofovir (TDF) in their regimens, and 80% of them had HIV RNA < 40 copies/mL. The 3TC resistance mutations rtL180M and rtM204V were observed in 10 (47.6%) of the 21 patients, while 5 patients (23.8%) had rtV173, rtL80I, and rtM204V mutations.
A high proportion of HBV-HIV co-infected patients with detectable viremia on 3TC-containing ART had resistance mutations despite good ART adherence as determined by HIV RNA suppression. This study emphasizes the need for dual therapy as part of a fully suppressive ART in all HBV-HIV co-infected patients in Ghana.
Url:
DOI: 10.3851/IMP3055
PubMed: 27167598
PubMed Central: 5106338
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000385
- to stream Pmc, to step Curation: 000384
- to stream Pmc, to step Checkpoint: 000093
- to stream PubMed, to step Corpus: 000465
- to stream PubMed, to step Curation: 000465
- to stream PubMed, to step Checkpoint: 000465
- to stream Ncbi, to step Merge: 000977
- to stream Ncbi, to step Curation: 000977
- to stream Ncbi, to step Checkpoint: 000977
Links to Exploration step
PMC:5106338Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">HBV genotypes and drug resistance mutations in antiretroviral treatment-naïve and treatment-experienced HBV-HIV co-infected patients</title>
<author><name sortKey="Archampong, Timothy N A" sort="Archampong, Timothy N A" uniqKey="Archampong T" first="Timothy N. A." last="Archampong">Timothy N. A. Archampong</name>
<affiliation wicri:level="4"><nlm:aff id="A1">Department of Medicine and Therapeutics, School of Medicine and Dentistry, College of Health Sciences, University of Ghana, Accra, Ghana</nlm:aff>
<country xml:lang="fr">Ghana</country>
<wicri:regionArea>Department of Medicine and Therapeutics, School of Medicine and Dentistry, College of Health Sciences, University of Ghana, Accra</wicri:regionArea>
<placeName><settlement type="city">Accra</settlement>
<region nuts="2">Région du Grand Accra</region>
</placeName>
<orgName type="university">Université du Ghana</orgName>
</affiliation>
<affiliation wicri:level="3"><nlm:aff id="A2">Korle-Bu Teaching Hospital, Accra, Ghana</nlm:aff>
<country xml:lang="fr">Ghana</country>
<wicri:regionArea>Korle-Bu Teaching Hospital, Accra</wicri:regionArea>
<placeName><settlement type="city">Accra</settlement>
<region nuts="2">Région du Grand Accra</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Boyce, Ceejay L" sort="Boyce, Ceejay L" uniqKey="Boyce C" first="Ceejay L." last="Boyce">Ceejay L. Boyce</name>
<affiliation wicri:level="2"><nlm:aff id="A3">Division of Digestive Disease, University of Cincinnati College of Medicine, Cincinnati, OH, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Division of Digestive Disease, University of Cincinnati College of Medicine, Cincinnati, OH</wicri:regionArea>
<placeName><region type="state">Ohio</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lartey, Margaret" sort="Lartey, Margaret" uniqKey="Lartey M" first="Margaret" last="Lartey">Margaret Lartey</name>
<affiliation wicri:level="4"><nlm:aff id="A1">Department of Medicine and Therapeutics, School of Medicine and Dentistry, College of Health Sciences, University of Ghana, Accra, Ghana</nlm:aff>
<country xml:lang="fr">Ghana</country>
<wicri:regionArea>Department of Medicine and Therapeutics, School of Medicine and Dentistry, College of Health Sciences, University of Ghana, Accra</wicri:regionArea>
<placeName><settlement type="city">Accra</settlement>
<region nuts="2">Région du Grand Accra</region>
</placeName>
<orgName type="university">Université du Ghana</orgName>
<placeName><settlement type="city">Accra</settlement>
<settlement type="town">Legon (Ghana)</settlement>
<region type="region">Région du Grand Accra</region>
</placeName>
<orgName type="university" n="3">Université du Ghana</orgName>
</affiliation>
<affiliation wicri:level="3"><nlm:aff id="A2">Korle-Bu Teaching Hospital, Accra, Ghana</nlm:aff>
<country xml:lang="fr">Ghana</country>
<wicri:regionArea>Korle-Bu Teaching Hospital, Accra</wicri:regionArea>
<placeName><settlement type="city">Accra</settlement>
<region nuts="2">Région du Grand Accra</region>
</placeName>
<placeName><settlement type="city">Accra</settlement>
<settlement type="town">Legon (Ghana)</settlement>
<region type="region">Région du Grand Accra</region>
</placeName>
<orgName type="university" n="3">Université du Ghana</orgName>
</affiliation>
</author>
<author><name sortKey="Sagoe, Kwamena W" sort="Sagoe, Kwamena W" uniqKey="Sagoe K" first="Kwamena W." last="Sagoe">Kwamena W. Sagoe</name>
<affiliation wicri:level="4"><nlm:aff id="A4">Department of Medical Microbiology, School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, Accra, Ghana</nlm:aff>
<country xml:lang="fr">Ghana</country>
<wicri:regionArea>Department of Medical Microbiology, School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, Accra</wicri:regionArea>
<placeName><settlement type="city">Accra</settlement>
<region nuts="2">Région du Grand Accra</region>
</placeName>
<orgName type="university">Université du Ghana</orgName>
</affiliation>
</author>
<author><name sortKey="Obo Akwa, Adjoa" sort="Obo Akwa, Adjoa" uniqKey="Obo Akwa A" first="Adjoa" last="Obo-Akwa">Adjoa Obo-Akwa</name>
<affiliation wicri:level="4"><nlm:aff id="A1">Department of Medicine and Therapeutics, School of Medicine and Dentistry, College of Health Sciences, University of Ghana, Accra, Ghana</nlm:aff>
<country xml:lang="fr">Ghana</country>
<wicri:regionArea>Department of Medicine and Therapeutics, School of Medicine and Dentistry, College of Health Sciences, University of Ghana, Accra</wicri:regionArea>
<placeName><settlement type="city">Accra</settlement>
<region nuts="2">Région du Grand Accra</region>
</placeName>
<orgName type="university">Université du Ghana</orgName>
</affiliation>
</author>
<author><name sortKey="Kenu, Ernest" sort="Kenu, Ernest" uniqKey="Kenu E" first="Ernest" last="Kenu">Ernest Kenu</name>
<affiliation wicri:level="3"><nlm:aff id="A2">Korle-Bu Teaching Hospital, Accra, Ghana</nlm:aff>
<country xml:lang="fr">Ghana</country>
<wicri:regionArea>Korle-Bu Teaching Hospital, Accra</wicri:regionArea>
<placeName><settlement type="city">Accra</settlement>
<region nuts="2">Région du Grand Accra</region>
</placeName>
</affiliation>
<affiliation wicri:level="4"><nlm:aff id="A5">School of Public Health, College of Health Sciences, University of Ghana</nlm:aff>
<country>Ghana</country>
<placeName><settlement type="city">Accra</settlement>
<settlement type="town">Legon (Ghana)</settlement>
<region type="region">Région du Grand Accra</region>
</placeName>
<orgName type="university">Université du Ghana</orgName>
</affiliation>
</author>
<author><name sortKey="Blackard, Jason T" sort="Blackard, Jason T" uniqKey="Blackard J" first="Jason T." last="Blackard">Jason T. Blackard</name>
<affiliation wicri:level="2"><nlm:aff id="A3">Division of Digestive Disease, University of Cincinnati College of Medicine, Cincinnati, OH, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Division of Digestive Disease, University of Cincinnati College of Medicine, Cincinnati, OH</wicri:regionArea>
<placeName><region type="state">Ohio</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kwara, Awewura" sort="Kwara, Awewura" uniqKey="Kwara A" first="Awewura" last="Kwara">Awewura Kwara</name>
<affiliation wicri:level="2"><nlm:aff id="A6">Warren Alpert Medical School of Brown University, Providence, RI, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Warren Alpert Medical School of Brown University, Providence, RI</wicri:regionArea>
<placeName><region type="state">Rhode Island</region>
</placeName>
</affiliation>
<affiliation wicri:level="2"><nlm:aff id="A7">The Miriam Hospital, Providence, RI, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>The Miriam Hospital, Providence, RI</wicri:regionArea>
<placeName><region type="state">Rhode Island</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">27167598</idno>
<idno type="pmc">5106338</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5106338</idno>
<idno type="RBID">PMC:5106338</idno>
<idno type="doi">10.3851/IMP3055</idno>
<date when="2016">2016</date>
<idno type="wicri:Area/Pmc/Corpus">000385</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000385</idno>
<idno type="wicri:Area/Pmc/Curation">000384</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000384</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000093</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000093</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="wicri:Area/PubMed/Corpus">000465</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000465</idno>
<idno type="wicri:Area/PubMed/Curation">000465</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000465</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000465</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000465</idno>
<idno type="wicri:Area/Ncbi/Merge">000977</idno>
<idno type="wicri:Area/Ncbi/Curation">000977</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000977</idno>
<idno type="wicri:doubleKey">1359-6535:2016:Archampong T:hbv:genotypes:and</idno>
<idno type="wicri:Area/Main/Merge">000195</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">HBV genotypes and drug resistance mutations in antiretroviral treatment-naïve and treatment-experienced HBV-HIV co-infected patients</title>
<author><name sortKey="Archampong, Timothy N A" sort="Archampong, Timothy N A" uniqKey="Archampong T" first="Timothy N. A." last="Archampong">Timothy N. A. Archampong</name>
<affiliation wicri:level="4"><nlm:aff id="A1">Department of Medicine and Therapeutics, School of Medicine and Dentistry, College of Health Sciences, University of Ghana, Accra, Ghana</nlm:aff>
<country xml:lang="fr">Ghana</country>
<wicri:regionArea>Department of Medicine and Therapeutics, School of Medicine and Dentistry, College of Health Sciences, University of Ghana, Accra</wicri:regionArea>
<placeName><settlement type="city">Accra</settlement>
<region nuts="2">Région du Grand Accra</region>
</placeName>
<orgName type="university">Université du Ghana</orgName>
</affiliation>
<affiliation wicri:level="3"><nlm:aff id="A2">Korle-Bu Teaching Hospital, Accra, Ghana</nlm:aff>
<country xml:lang="fr">Ghana</country>
<wicri:regionArea>Korle-Bu Teaching Hospital, Accra</wicri:regionArea>
<placeName><settlement type="city">Accra</settlement>
<region nuts="2">Région du Grand Accra</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Boyce, Ceejay L" sort="Boyce, Ceejay L" uniqKey="Boyce C" first="Ceejay L." last="Boyce">Ceejay L. Boyce</name>
<affiliation wicri:level="2"><nlm:aff id="A3">Division of Digestive Disease, University of Cincinnati College of Medicine, Cincinnati, OH, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Division of Digestive Disease, University of Cincinnati College of Medicine, Cincinnati, OH</wicri:regionArea>
<placeName><region type="state">Ohio</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lartey, Margaret" sort="Lartey, Margaret" uniqKey="Lartey M" first="Margaret" last="Lartey">Margaret Lartey</name>
<affiliation wicri:level="4"><nlm:aff id="A1">Department of Medicine and Therapeutics, School of Medicine and Dentistry, College of Health Sciences, University of Ghana, Accra, Ghana</nlm:aff>
<country xml:lang="fr">Ghana</country>
<wicri:regionArea>Department of Medicine and Therapeutics, School of Medicine and Dentistry, College of Health Sciences, University of Ghana, Accra</wicri:regionArea>
<placeName><settlement type="city">Accra</settlement>
<region nuts="2">Région du Grand Accra</region>
</placeName>
<orgName type="university">Université du Ghana</orgName>
<placeName><settlement type="city">Accra</settlement>
<settlement type="town">Legon (Ghana)</settlement>
<region type="region">Région du Grand Accra</region>
</placeName>
<orgName type="university" n="3">Université du Ghana</orgName>
</affiliation>
<affiliation wicri:level="3"><nlm:aff id="A2">Korle-Bu Teaching Hospital, Accra, Ghana</nlm:aff>
<country xml:lang="fr">Ghana</country>
<wicri:regionArea>Korle-Bu Teaching Hospital, Accra</wicri:regionArea>
<placeName><settlement type="city">Accra</settlement>
<region nuts="2">Région du Grand Accra</region>
</placeName>
<placeName><settlement type="city">Accra</settlement>
<settlement type="town">Legon (Ghana)</settlement>
<region type="region">Région du Grand Accra</region>
</placeName>
<orgName type="university" n="3">Université du Ghana</orgName>
</affiliation>
</author>
<author><name sortKey="Sagoe, Kwamena W" sort="Sagoe, Kwamena W" uniqKey="Sagoe K" first="Kwamena W." last="Sagoe">Kwamena W. Sagoe</name>
<affiliation wicri:level="4"><nlm:aff id="A4">Department of Medical Microbiology, School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, Accra, Ghana</nlm:aff>
<country xml:lang="fr">Ghana</country>
<wicri:regionArea>Department of Medical Microbiology, School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, Accra</wicri:regionArea>
<placeName><settlement type="city">Accra</settlement>
<region nuts="2">Région du Grand Accra</region>
</placeName>
<orgName type="university">Université du Ghana</orgName>
</affiliation>
</author>
<author><name sortKey="Obo Akwa, Adjoa" sort="Obo Akwa, Adjoa" uniqKey="Obo Akwa A" first="Adjoa" last="Obo-Akwa">Adjoa Obo-Akwa</name>
<affiliation wicri:level="4"><nlm:aff id="A1">Department of Medicine and Therapeutics, School of Medicine and Dentistry, College of Health Sciences, University of Ghana, Accra, Ghana</nlm:aff>
<country xml:lang="fr">Ghana</country>
<wicri:regionArea>Department of Medicine and Therapeutics, School of Medicine and Dentistry, College of Health Sciences, University of Ghana, Accra</wicri:regionArea>
<placeName><settlement type="city">Accra</settlement>
<region nuts="2">Région du Grand Accra</region>
</placeName>
<orgName type="university">Université du Ghana</orgName>
</affiliation>
</author>
<author><name sortKey="Kenu, Ernest" sort="Kenu, Ernest" uniqKey="Kenu E" first="Ernest" last="Kenu">Ernest Kenu</name>
<affiliation wicri:level="3"><nlm:aff id="A2">Korle-Bu Teaching Hospital, Accra, Ghana</nlm:aff>
<country xml:lang="fr">Ghana</country>
<wicri:regionArea>Korle-Bu Teaching Hospital, Accra</wicri:regionArea>
<placeName><settlement type="city">Accra</settlement>
<region nuts="2">Région du Grand Accra</region>
</placeName>
</affiliation>
<affiliation wicri:level="4"><nlm:aff id="A5">School of Public Health, College of Health Sciences, University of Ghana</nlm:aff>
<country>Ghana</country>
<placeName><settlement type="city">Accra</settlement>
<settlement type="town">Legon (Ghana)</settlement>
<region type="region">Région du Grand Accra</region>
</placeName>
<orgName type="university">Université du Ghana</orgName>
</affiliation>
</author>
<author><name sortKey="Blackard, Jason T" sort="Blackard, Jason T" uniqKey="Blackard J" first="Jason T." last="Blackard">Jason T. Blackard</name>
<affiliation wicri:level="2"><nlm:aff id="A3">Division of Digestive Disease, University of Cincinnati College of Medicine, Cincinnati, OH, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Division of Digestive Disease, University of Cincinnati College of Medicine, Cincinnati, OH</wicri:regionArea>
<placeName><region type="state">Ohio</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kwara, Awewura" sort="Kwara, Awewura" uniqKey="Kwara A" first="Awewura" last="Kwara">Awewura Kwara</name>
<affiliation wicri:level="2"><nlm:aff id="A6">Warren Alpert Medical School of Brown University, Providence, RI, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Warren Alpert Medical School of Brown University, Providence, RI</wicri:regionArea>
<placeName><region type="state">Rhode Island</region>
</placeName>
</affiliation>
<affiliation wicri:level="2"><nlm:aff id="A7">The Miriam Hospital, Providence, RI, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>The Miriam Hospital, Providence, RI</wicri:regionArea>
<placeName><region type="state">Rhode Island</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Antiviral therapy</title>
<idno type="ISSN">1359-6535</idno>
<idno type="eISSN">2040-2058</idno>
<imprint><date when="2016">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><sec id="S1"><title>Background</title>
<p id="P1">The presence of hepatitis B virus (HBV) resistance mutations upon initiation or during antiretroviral therapy (ART) in HIV co-infected patients is an important determinant of treatment response. The main objective of the study was to determine the prevalence of HBV resistance mutations in antiretroviral treatment-naïve and treatment-experienced HBV-HIV co-infected Ghanaian patients with detectable HBV viremia.</p>
</sec>
<sec id="S2"><title>Methods</title>
<p id="P2">HBV-HIV co-infected patients who were ART-naïve or had received at least 9 months of lamivudine (3TC)-containing ART were enrolled in a cross-sectional study. Demographic and clinical data were collected and HBV DNA quantified. Partial HBV sequences were amplified by PCR and sequenced bi-directionally to obtain a 2.1-2.2 kb fragment for phylogenetic analysis of HBV genotypes and evaluation of drug resistance mutations.</p>
</sec>
<sec id="S3"><title>Results</title>
<p id="P3">Of the 100 HBV-HIV co-infected study patients, 75 were successfully PCR-amplified, and 63 were successfully sequenced. Of these 63 patients, 27 (42.9%) were ART-experienced, and 58 (92.1%) had HBV genotype E. No resistance mutations were observed in the 36 ART-naïve patients, while 21 (77.8%) of 27 treatment-experienced patients had resistance mutations. All patients with resistance mutations had no tenofovir (TDF) in their regimens, and 80% of them had HIV RNA < 40 copies/mL. The 3TC resistance mutations rtL180M and rtM204V were observed in 10 (47.6%) of the 21 patients, while 5 patients (23.8%) had rtV173, rtL80I, and rtM204V mutations.</p>
</sec>
<sec id="S4"><title>Discussion</title>
<p id="P4">A high proportion of HBV-HIV co-infected patients with detectable viremia on 3TC-containing ART had resistance mutations despite good ART adherence as determined by HIV RNA suppression. This study emphasizes the need for dual therapy as part of a fully suppressive ART in all HBV-HIV co-infected patients in Ghana.</p>
</sec>
</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/SidaGhanaV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000195 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 000195 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= SidaGhanaV1 |flux= Main |étape= Merge |type= RBID |clé= PMC:5106338 |texte= HBV genotypes and drug resistance mutations in antiretroviral treatment-naïve and treatment-experienced HBV-HIV co-infected patients }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:27167598" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \ | NlmPubMed2Wicri -a SidaGhanaV1
![]() | This area was generated with Dilib version V0.6.31. | ![]() |